X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Categories:
Serum-free and Speciality Media
Research Areas:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Stem Cells

Citations (96)

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3D Cell Culture , Serum-free and Speciality Media 
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Primary Cells and Media, Serum-free and Speciality Media, Transfection 
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Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
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Primary Cells and Media, Serum-free and Speciality Media, Transfection 
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Primary Cells and Media, Serum-free and Speciality Media, Transfection 
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Primary Cells and Media, Serum-free and Speciality Media 
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Primary Cells and Media, Serum-free and Speciality Media, Transfection 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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J Immunol (2014) 139(9): 4739-47 
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Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Circ Res (2014) 111(7): 854-862 
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Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
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PLoS ONE (2014) 9 (2): e87210 
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Serum-free and Speciality Media 
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Journal of Neuroinflammation (2014) 11: 130 
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Serum-free and Speciality Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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J Mol Biol (2013) 5(6): 358-68 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Invest Ophthalmol Vis Sci (2013) 54(10): 6745-56 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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PLoS Pathog (2013) 09(5): e1003366 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Haematologica (2013) 98(8): 1291-9 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Infect Immun (2013) 81(8): 2997-3008 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Haematologica (2013) 98(8): 1300-8 
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Classical Media and Reagents, Serum-free and Speciality Media 
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In:
Invest Ophthalmol Vis Sci (2013) 54(7): 5087-96 
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PLoS ONE (2013) 8(6): e68201 
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Kim S1, Park SY, Kim SY, Bae DJ, Pyo JH, Hong M, Kim IS. 
In:
Mol Cell Biol (2012) 32(14): 2698-708 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yeste A1, Nadeau M, Burns EJ, Weiner HL, Quintana FJ. 
In:
Proc Natl Acad Sci USA (2012) 109(28): 11270-5 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tuomela S1, Salo V, Tripathi SK, Chen Z, Laurila K, Gupta B, Äijö T, Oikari L, Stockinger B, Lähdesmäki H, Lahesmaa R. 
In:
Blood (2012) 119(23): e151-60 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Klymiuk N1, van Buerck L, Bähr A, Offers M, Kessler B, Wuensch A, Kurome M, Thormann M, Lochner K, Nagashima H, Herbach N, Wanke R, Seissler J, Wolf E. 
In:
Diabetes (2012) 61(6): 1527-32 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
An HJ1, Gip P, Kim J, Wu S, Park KW, McVaugh CT, Schaffer DV, Bertozzi CR, Lebrilla CB. 
In:
Mol Cell Proteomics (2012) 11(4): M111.010660 
Categories:
Serum-free and Speciality Media 
Authors:
Chatterjee M1, Rauen T, Kis-Toth K, Kyttaris VC, Hedrich CM, Terhorst C, Tsokos GC. 
In:
J Immunol (2012) 188(3): 1206-12 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Desmedt C1, Majjaj S, Kheddoumi N, Singhal SK, Haibe-Kains B, El Ouriaghli F, Chaboteaux C, Michiels S, Lallemand F, Journe F, Duvillier H, Loi S, Quackenbush J, Dekoninck S, Blanpain C, Lagneaux L, Houhou N, Delorenzi M, Larsimont D, Piccart M, Sotiriou C. 
In:
Clin Cancer Res (2012) 18(4): 1004-14 
Categories:
Serum-free and Speciality Media 
Authors:
Renier J. Brentjens, Isabelle Rivière, Jae H. Park, Marco L. Davila, Xiuyan Wang, Jolanta Stefanski, Clare Taylor, Raymond Yeh, Shirley Bartido, Oriana Borquez-Ojeda, Malgorzata Olszewska, Yvette Bernal, Hollie Pegram, Mark Przybylowski, Daniel Hollyman, Yelena Usachenko, Domenick Pirraglia, James Hosey, Elmer Santos, Elizabeth Halton, Peter Maslak, David Scheinberg, Joseph Jurcic, Mark Heaney, Glenn Heller, Mark Frattini, Michel Sadelain 
In:
Blood (2011) 118 (18): 4817-4828 
Categories:
Serum-free and Speciality Media, Transfection 
Authors:
Hook L1, Vives J, Fulton N, Leveridge M, Lingard S, Bootman MD, Falk A, Pollard SM, Allsopp TE, Dalma-Weiszhausz D, Tsukamoto A, Uchida N, Gorba T. 
In:
Neurochemistry International (2011) 59 (2011): 432-444 
Categories:
 
Authors:
Keerthivasan S1, Suleiman R, Lawlor R, Roderick J, Bates T, Minter L, Anguita J, Juncadella I, Nickoloff BJ, Le Poole IC, Miele L, Osborne BA 
In:
J Immunol (2011) 187(2): 692-701 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Poole E, McGregor Dallas SR, Colston J, Joseph RS, Sinclair J 
In:
J Gen Virol (2011) 92(7): 1539-49 
Categories:
Serum-free and Speciality Media 
Authors:
Lisboa FA, Warren J, Sulkowski G, Aparicio M, David G, Zudaire E, Dveksler GS 
In:
J Biol Chem (2011) 286(9): 7577-86 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schmidt S1, Tramsen L, Hanisch M, Latgé JP, Huenecke S, Koehl U, Lehrnbecher T. 
In:
J Infect Dis (2011) 203(3): 430-5 
Categories:
Transfection 
Authors:
Holt N, Wang J, Kim K, Friedman G, Wang X, Taupin V, Crooks GM, Kohn DB, Gregory PD, Holmes MC, Cannon PM. 
In:
Nat Biotechnol (2010) 28: 839-847 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A. 
In:
Blood (2008) 111(3): 1428-36 
Categories:
Serum-free and Speciality Media 
Authors:
Sung Jung Kim, Betty Diamond 
In:
J Immunol Methods (2007) 323: 2007 
Categories:
Serum-free and Speciality Media, Transfection 
Authors:
Kubach J, Lutter P, Bopp T, Stoll S, Becker C, Huter E, Richter C, Weingarten P, Warger T, Knop J, Mullner S, Wijdenes J, Schild H, Schmitt E, Jonuleit H 
In:
Blood (2007) 110(5): 1550-8 
Categories:
Serum-free and Speciality Media 
Authors:
Li H1, Yu JP, Cao S, Wei F, Zhang P, An XM, Huang ZT, Ren XB. 
In:
J Clin Immunol (2007) 27 (3): 317-26 
Categories:
Serum-free and Speciality Media 
Authors:
Chan L, Hardwick N, Darling D, Galea-Lauri J, Gäken J, Devereux S, Kemeny M, Mufti G, Farzaneh F. 
In:
Mol Ther (2005) 11(1): 120-131 
Categories:
Serum-free and Speciality Media 
Authors:
Laport GG, Levine BL, Stadtmauer EA, Schuster SJ, Luger SM, Grupp S, Bunin N,  Strobl FJ, Cotte J, Zheng Z, Gregson B, Rivers P, Vonderheide RH, Liebowitz DN, Porter DL, June CH. 
In:
Blood (2003) 102 (6): 2004-13 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bruserud Ø, Hovland R, Wergeland L, Huang TS, Gjertsen BT. 
In:
Haematologica (2003) 88(4): 416-28 
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