T cell, mouse - C57BL/6

T lymphocytes isolated from mouse spleen.

Cell Type:
T Cell
Tissue Origin:
blood
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

TheraPEAK® X-VIVO® Media is a high-performing media series providing nutritionally adequate and balanced environments for a variety of hematopoietic cells including: 

  • T Cells
  • CAR-T cells
  • Peripheral blood lymphocytes (PBL)
  • Tumor infiltrating lymphocytes (TIL)
  • Human monocytes and macrophages
  • Lymphokine-activated killer cells (LAK)
  • Hematopoietic stem cells (HSCs)
  • Dendritic cells (DCs) 

Our scalable media are widely cited in scientific publications and proven to reliably work in many cell therapy applications around the world, from research stages to clinical trials to FDA-approved cell therapies. TheraPEAK® Products are manufactured according to GMP standards and can be safely and confidently used in your clinical processes. Our manufacturing sites are FDA registered with an ISO 13485 certified quality management system. 


There are two main versions of this media.

TheraPEAK® X-VIVO® 10 Media are  optimized for slower growing, less mature cells, such as hematopoietic stem cells

TheraPEAK® X-VIVO® 15 Media are most appropriate for rapidly growing cells, specifically cells of the immune system

TheraPEAK® X-VIVO® 10 Media and 15 Media are also available with recombinant transferrin instead of native human transferrin

Please note: X-VIVO™ 20 is currently available as an RUO grade catalog item. 

Storage: 2-8C, protect from light.

Catalog Offerings:

TheraPEAK® X-VIVO® Medium with native transferrin

Cat. No. NA

Cat.CCat. No. EU

Product

Size

BP04-743Q                            

 

           BEBP04-743Q

TheraPEAK® X-VIVO® 10 Medium without gentamicin and phenol red, contains native transferrin

1 L bottle

BP04-744Q

 

BEBP04-744Q

TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin

1 L bottle

08-879H

08-879P1

 

BE08-879H

BE08-879P1

TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin

5 L bag

1 L bag

TheraPEAK™ X-VIVO™ Medium with recombinant transferrin

Cat. No. NA

Cat. No. EU

Product

Size

N/A

BEBP02-055Q

TheraPEAK® X-VIVO® 10 Medium with L-glutamine, without gentamicin or phenol red, contains recombinant transferrin

1 L bottle

BP02-054Q

BP02-054P1

BEBP02-054Q

BEBP02-054P1

TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains recombinant transferrin

1 L bottle

1 L bag

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3

DN-100

2e6 35-45% 25-30% Plasmid (general) 0.5 µg 20 µl 4D X-Unit
T cell, mouse X-001 5e6 21-33% 42-48% Plasmid (general) 2.5 µg 100 µl I/II/2b
T cell, mouse X-001 5e6 21-35% 21-29% Plasmid (general) 2.5 µg 100 µl I/II/2b
T cell, mouse X-001 5e6 18-28% 35-47% Plasmid (general) 2.5 µg 100 µl I/II/2b
T cell, mouse X-001 5e6 15-25% 12-21% Plasmid (general) 2.5 µg 100 µl I/II/2b
P3 96-DN-100 2e6 34-52% 14-32% Plasmid (general) 0.5 µg 20 µl 96-well

Citations (33)

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Primary Cells and Media, Transfection 
Authors:
Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, Xie WH, Allain V, Steinhart Z, Chang C, Talbot A, Kim S, Rosales A, Havlik LP, Pimentel H, Asokan A, Eyquem J 
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Cell (2023) 186(2): 446-460 
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Transfection, Cell Lines and Primary Cancer Cells 
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Frontiers in Immunology (2022) 13: 777113 
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Primary Cells and Media, Transfection 
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Curr Protoc Immunol (2019) 124(1): e69 
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Primary Cells and Media, Transfection 
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Nature (2019) 565 (7738): 246-250 
Categories:
Primary Cells and Media, Transfection 
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J Exp Med (2018) 215(3): 985-997 
Categories:
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PLoS ONE (2013) 8(6): e68201 
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Nat Commun. (2013) 4: 1685 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
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Categories:
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J Immunol (2008) 181(7): 4832-9 
Categories:
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J Immunol (2008) 181(3): 1673-82 
Categories:
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Mol Pharmacol (2008) 73(6): 1722-35 
Categories:
Transfection 
Authors:
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Categories:
Transfection 
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J Immunol (2008) 180(6): 3746-56 
Categories:
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Authors:
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In:
J Immunol (2008) 180(3): 1584-91 
Categories:
Transfection 
Authors:
Chung JS, Dougherty I, Cruz PD Jr, Ariizumi K 
In:
J Immunol (2007) 179(9): 5778-84 
Categories:
Transfection 
Authors:
Chandok MR, Okoye FI, Ndejembi MP, Farber DL 
In:
J Immunol (2007) 179(6): 3689-98 
Categories:
Transfection 
Authors:
Voskoboinik I, Sutton VR, Ciccone A, House C, Chia J, Darcy PK, Yagita H, Trapani JA 
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Blood (2007) 110(4): 1184-90 
Categories:
Transfection 
Authors:
Schoenborn JR, Dorschner MO, Sekimata M, Santer DM, Shnyreva M, Fitzpatrick DR, Stamatoyonnapoulos JA, Wilson CB 
In:
Nat Immunol (2007) 8(7): 732-42 
Categories:
Transfection 
Authors:
Sasaki K, Zhu X, Vasquez C, Nishimura F, Dusak JE, Huang J, Fujita M, Wesa A, Potter DM, Walker PR, Storkus WJ, Okada H 
In:
Cancer Res (2007) 67(13): 6451-8 
Categories:
Transfection 
Authors:
Bopp T, Becker C, Klein M, Klein-Heßling S, Palmetshofer A, Serfling E, Heib V, Becker M, Kubach J, Schmitt S, Stoll S, Schild H, Staege MS, Stassen M, Jonuleit H, Schmitt E 
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J Exp Med (2007) 204(6): 1303-1310 
Categories:
Transfection 
Authors:
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In:
J Immunol (2007) 178(12): 7814-21 
Categories:
Transfection 
Authors:
Hosokawa H, Kimura MY, Shinnakasu R, Suzuki A, Miki T, Koseki H, van Lohuizen M, Yamashita M, Nakayama T 
In:
J Immunol (2006) 177(11): 7656-64 
Categories:
Transfection 
Authors:
Sabbagh L, Srokowski CC, Pulle G, Snell LM, Sedgmen BJ, Liu Y, Tsitsikov EN, Watts TH 
In:
Proc Natl Acad Sci USA (2006) 103(49): 18703-8 
Categories:
Transfection 
Authors:
Huan C, Kelly ML, Steele R, Shapira I, Gottesman SR, Roman CA 
In:
Nat Immunol (2006) 7(10): 1082-1091 
Categories:
Transfection 
Authors:
Singh N, Seki Y, Takami M, Baban B, Chandler PR, Khosravi D, Zheng X, Takezaki M, Lee JR, Mellor AL, Bollag WB, Iwashima M 
In:
Nat Methods (2006) 3(8): 629-36 
Categories:
Transfection 
Authors:
Coste A, Antal MC, Chan S, Kastner P, Mark M, O'malley BW, Auwerx J 
In:
EMBO J (2006) 25(11): 2453-64 
Categories:
Transfection 
Authors:
Au-Yeung BB, Katzman SD, Fowell DJ 
In:
J Immunol (2006) 176(7): 3895-9 
Categories:
Transfection 
Authors:
Lin L, Peng SL 
In:
J Immunol (2006) 176(8): 4793-803 
Categories:
Transfection 
Authors:
Marti F, Garcia GG, Lapinski PE, Macgregor JN and King PD 
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J Exp Med (2006) 203(2): 281-7 
Categories:
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Authors:
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EMBO J (2006) 25(4): 774-84 
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EMBO J (2005) 24(20): 3555-3564 
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