cells

T lymphocytes isolated from mouse spleen.

Cell Type:

T Cell

Tissue Origin:

blood

Species:

mouse

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

TheraPEAK® X-VIVO®

TheraPEAK® X-VIVO® Media is a high-performing media series providing nutritionally adequate and balanced environments for a variety of hematopoietic cells including:

T Cells
CAR-T cells
Peripheral blood lymphocytes (PBL)
Tumor infiltrating lymphocytes (TIL)
Human monocytes and macrophages
Lymphokine-activated killer cells (LAK)
Hematopoietic stem cells (HSCs)
Dendritic cells (DCs)

Our scalable media are widely cited in scientific publications and proven to reliably work in many cell therapy applications around the world, from research stages to clinical trials to FDA-approved cell therapies. TheraPEAK® Products are manufactured according to GMP standards and can be safely and confidently used in your clinical processes. Our manufacturing sites are FDA registered with an ISO 13485 certified quality management system.

There are two main versions of this media.

TheraPEAK® X-VIVO® 10 Media are optimized for slower growing, less mature cells, such as hematopoietic stem cells

TheraPEAK® X-VIVO® 15 Media are most appropriate for rapidly growing cells, specifically cells of the immune system

TheraPEAK® X-VIVO® 10 Media and 15 Media are also available with recombinant transferrin instead of native human transferrin

Please note: X-VIVO™ 20 is currently available as an RUO grade catalog item.

Storage: 2-8C, protect from light.

Catalog Offerings:

TheraPEAK® X-VIVO® Medium with native transferrin
Cat. No. NA	Cat.CCat. No. EU	Product	Size
BP04-743Q	BEBP04-743Q	TheraPEAK® X-VIVO® 10 Medium without gentamicin and phenol red, contains native transferrin	1 L bottle
BP04-744Q	BEBP04-744Q	TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin	1 L bottle
08-879H 08-879P1	BE08-879H BE08-879P1	TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin	5 L bag 1 L bag
TheraPEAK™ X-VIVO™ Medium with recombinant transferrin
Cat. No. NA	Cat. No. EU	Product	Size
N/A	BEBP02-055Q	TheraPEAK® X-VIVO® 10 Medium with L-glutamine, without gentamicin or phenol red, contains recombinant transferrin	1 L bottle
BP02-054Q BP02-054P1	BEBP02-054Q BEBP02-054P1	TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains recombinant transferrin	1 L bottle 1 L bag

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
P3	DN-100	2e6	35-45%	25-30%	Plasmid (general)	0.5 µg	20 µl	4D X-Unit
T cell, mouse	X-001	5e6	21-33%	42-48%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
T cell, mouse	X-001	5e6	21-35%	21-29%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
T cell, mouse	X-001	5e6	18-28%	35-47%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
T cell, mouse	X-001	5e6	15-25%	12-21%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
P3	96-DN-100	2e6	34-52%	14-32%	Plasmid (general)	0.5 µg	20 µl	96-well

Citations (33)

An evolved AAV variant enables efficient genetic engineering of murine T cells

Categories:

Primary Cells and Media, Transfection

Authors:

Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, Xie WH, Allain V, Steinhart Z, Chang C, Talbot A, Kim S, Rosales A, Havlik LP, Pimentel H, Asokan A, Eyquem J

In:

Cell (2023) 186(2): 446-460

Naïve Primary Mouse CD8+ T Cells Retain In Vivo Immune Responsiveness After Electroporation-Based CRISPR/Cas9 Genetic Engineering

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Pfenninger P, Yerly L, Abe J

In:

Frontiers in Immunology (2022) 13: 777113

Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.

Categories:

Primary Cells and Media, Transfection

Authors:

Oh SA, Seki A, Rutz S.

In:

Curr Protoc Immunol (2019) 124(1): e69

Brain regulatory T cells suppress astrogliosis and potentiate neurological recovery.

Categories:

Primary Cells and Media, Transfection

Authors:

Ito M, Komai K, Mise-Omata S, Iizuka-Koga M, Noguchi Y, Kondo T, Sakai R, Matsuo K, Nakayama T, Yoshie O, Nakatsukasa H, Chikuma S, Shichita T, Yoshimura A.

In:

Nature (2019) 565 (7738): 246-250

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.

Categories:

Primary Cells and Media, Transfection

Authors:

Seki A, Rutz S.

In:

J Exp Med (2018) 215(3): 985-997

Comparison of Lentiviral and Sleeping Beauty Mediated alpha beta T Cell Receptor Gene Transfer

Categories:

Transfection

Authors:

Anne-Christine Field, Conrad Vink, Richard Gabriel, Roua Al-Subki, Manfred Schmidt, Nicholas Goulden, Hans Stauss, Adrian Thrasher, Emma Morris, Waseem Qasim

In:

PLoS ONE (2013) 8(6): e68201

Prostaglandin E2 promotes Th1 differentiation via synergistic amplification of IL-12 signalling by cAMP and PI3-kinase.

Categories:

Transfection

Authors:

Yao C1, Hirata T, Soontrapa K, Ma X, Takemori H, Narumiya S.

In:

Nat Commun. (2013) 4: 1685

Cutting edge: suppression of GM-CSF expression in murine and human T cells by IL-27.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Young A1, Linehan E, Hams E, O'Hara Hall AC, McClurg A, Johnston JA, Hunter CA, Fallon PG, Fitzgerald DC

In:

J Immunol (2012) 189 (5): 2079-83

Protein kinase C delta localizes to secretory lysosomes in CD8+ CTL and directly mediates TCR signals leading to granule exocytosis-mediated cytotoxicity

Categories:

Transfection

Authors:

Ma JS, Haydar TF, Radoja S

In:

J Immunol (2008) 181(7): 4716-22

NFATc1 regulates PD-1 expression upon T cell activation

Categories:

Transfection

Authors:

Oestreich KJ, Yoon H, Ahmed R, Boss JM

In:

J Immunol (2008) 181(7): 4832-9

IFN regulatory factor-1 negatively regulates CD4+ CD25+ regulatory T cell differentiation by repressing Foxp3 expression

Categories:

Transfection

Authors:

Fragale A, Gabriele L, Stellacci E, Borghi P, Perrotti E, Ilari R, Lanciotti A, Remoli AL, Venditti M, Belardelli F, Battistini A

In:

J Immunol (2008) 181(3): 1673-82

Primary peripheral T cells become susceptible to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)-mediated apoptosis in vitro upon activation and in the presence of dendritic cells

Categories:

Transfection

Authors:

Singh NP, Nagarkatti M, Nagarkatti P

In:

Mol Pharmacol (2008) 73(6): 1722-35

SNPs upstream of the minimal promoter control IL-2 expression and are candidates for the autoimmune disease-susceptibility locus Aod2/Idd3/Eae3

Categories:

Transfection

Authors:

Del Rio R, Noubade R, Subramanian M, Saligrama N, Diehl S, Rincon M, Teuscher C

In:

Genes Immun (2008) 9(2): 115-21

Loss of Suppressor of Cytokine Signaling 1 in Helper T Cells Leads to Defective Th17 Differentiation by Enhancing Antagonistic Effects of IFN-gamma on STAT3 and Smads

Categories:

Transfection

Authors:

Tanaka K, Ichiyama K, Hashimoto M, Yoshida H, Takimoto T, Takaesu G, Torisu T, Hanada T, Yasukawa H, Fukuyama S, Inoue H, Nakanishi Y, Kobayashi T, Yoshimura A

In:

J Immunol (2008) 180(6): 3746-56

A peptide that ameliorates lupus up-regulates the diminished expression of early growth response factors 2 and 3

Categories:

Transfection

Authors:

Sela U, Dayan M, Hershkoviz R, Lider O, Mozes E

In:

J Immunol (2008) 180(3): 1584-91

Syndecan-4 Mediates the Coinhibitory Function of DC-HIL on T Cell Activation

Categories:

Transfection

Authors:

Chung JS, Dougherty I, Cruz PD Jr, Ariizumi K

In:

J Immunol (2007) 179(9): 5778-84

A biochemical signature for rapid recall of memory CD4 T cells

Categories:

Transfection

Authors:

Chandok MR, Okoye FI, Ndejembi MP, Farber DL

In:

J Immunol (2007) 179(6): 3689-98

Perforin activity and immune homeostasis: the common A91V polymorphism in perforin results in both pre- and post-synaptic defects in function

Categories:

Transfection

Authors:

Voskoboinik I, Sutton VR, Ciccone A, House C, Chia J, Darcy PK, Yagita H, Trapani JA

In:

Blood (2007) 110(4): 1184-90

Comprehensive epigenetic profiling identifies multiple distal regulatory elements directing transcription of the gene encoding interferon-gamma

Categories:

Transfection

Authors:

Schoenborn JR, Dorschner MO, Sekimata M, Santer DM, Shnyreva M, Fitzpatrick DR, Stamatoyonnapoulos JA, Wilson CB

In:

Nat Immunol (2007) 8(7): 732-42

Preferential expression of very late antigen-4 on type 1 CTL cells plays a critical role in trafficking into central nervous system tumors

Categories:

Transfection

Authors:

Sasaki K, Zhu X, Vasquez C, Nishimura F, Dusak JE, Huang J, Fujita M, Wesa A, Potter DM, Walker PR, Storkus WJ, Okada H

In:

Cancer Res (2007) 67(13): 6451-8

Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression

Categories:

Transfection

Authors:

Bopp T, Becker C, Klein M, Klein-Heßling S, Palmetshofer A, Serfling E, Heib V, Becker M, Kubach J, Schmitt S, Stoll S, Schild H, Staege MS, Stassen M, Jonuleit H, Schmitt E

In:

J Exp Med (2007) 204(6): 1303-1310

Protein Kinase C Regulates Antigen Receptor-Induced Lytic Granule Polarization in Mouse CD8+ CTL

Categories:

Transfection

Authors:

Ma JS, Monu N, Shen DT, Mecklenbräuker I, Radoja N, Haydar TF, Leitges M, Frey AB, Vukmanovic S, Radoja S

In:

J Immunol (2007) 178(12): 7814-21

Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3

Categories:

Transfection

Authors:

Hosokawa H, Kimura MY, Shinnakasu R, Suzuki A, Miki T, Koseki H, van Lohuizen M, Yamashita M, Nakayama T

In:

J Immunol (2006) 177(11): 7656-64

A critical role for TNF receptor-associated factor 1 and Bim down-regulation in CD8 memory T cell survival

Categories:

Transfection

Authors:

Sabbagh L, Srokowski CC, Pulle G, Snell LM, Sedgmen BJ, Liu Y, Tsitsikov EN, Watts TH

In:

Proc Natl Acad Sci USA (2006) 103(49): 18703-8

Transcription factors TFE3 and TFEB are critical for CD40 ligand expression and thymus-dependent humoral immunity

Categories:

Transfection

Authors:

Huan C, Kelly ML, Steele R, Shapira I, Gottesman SR, Roman CA

In:

Nat Immunol (2006) 7(10): 1082-1091

Enrichment of regulatory CD4(+)CD25(+) T cells by inhibition of phospholipase D signaling

Categories:

Transfection

Authors:

Singh N, Seki Y, Takami M, Baban B, Chandler PR, Khosravi D, Zheng X, Takezaki M, Lee JR, Mellor AL, Bollag WB, Iwashima M

In:

Nat Methods (2006) 3(8): 629-36

Absence of the steroid receptor coactivator-3 induces B-cell lymphoma

Categories:

Transfection

Authors:

Coste A, Antal MC, Chan S, Kastner P, Mark M, O'malley BW, Auwerx J

In:

EMBO J (2006) 25(11): 2453-64

Cutting Edge: Itk-Dependent Signals Required for CD4+ T Cells to Exert, but Not Gain, Th2 Effector Function

Categories:

Transfection

Authors:

Au-Yeung BB, Katzman SD, Fowell DJ

In:

J Immunol (2006) 176(7): 3895-9

Coordination of NF-B and NFAT Antagonism by the Forkhead Transcription Factor Foxd1

Categories:

Transfection

Authors:

Lin L, Peng SL

In:

J Immunol (2006) 176(8): 4793-803

Essential role of the T cell-specific adapter protein in the activation of LCK in peripheral T cells

Categories:

Transfection

Authors:

Marti F, Garcia GG, Lapinski PE, Macgregor JN and King PD

In:

J Exp Med (2006) 203(2): 281-7

Recruitment and activation of PLCgamma1 in T cells: a new insight into old domains

Categories:

Transfection

Authors:

Braiman A, Barda-Saad M, Sommers CL, Samelson LE

In:

EMBO J (2006) 25(4): 774-84

Transcriptional repressor DREAM regulates T-lymphocyte proliferation and cytokine gene expression

Categories:

Transfection

Authors:

Savignac M, Pintado B, Gutierrez-Adan A, Palczewska M, Mellstrom B and Naranjo JR

In:

EMBO J (2005) 24(20): 3555-3564

c-Rel-Dependent Priming of Naive T Cells by Inflammatory Cytokines

Categories:

Transfection

Authors:

Banerjee D, Liou HC and Sen R

In:

Immunity (2005) 23(4): 445-458

+ Show All

T cell, mouse - C57BL/6

Recommended Media

Transfection Information

Citations (33)