media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

X-VIVO 10 - designed to support the generation of LAK cells in a serum-free environment.
X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15 with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20 with L-glutamine, gentamicin and phenol red

Recommended for Cells:

U-937 ; B cell, human ; T cell, human peripheral blood unstim. ; T cell, human stim. ; Dendritic cell (NHDC), human ; CD34+ cell, human ; Natural killer Cells (NK), human ; T cell, mouse - BALB/c ; Monocyte, human ; T cell, mouse - C57BL/6 ; Mast cell, human ; T cell, mouse, stim ; Granulocyte, human ; Bone Marrow, Human, Unprocessed ; Cytokine induced killer, human (CIK) ; Mononuclear, peripheral blood, human ; T cell, human cord blood unstim.

Categories:

Serum-free and Speciality Media

Research Areas:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Stem Cells

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Citations (99)

Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells

Categories:

3D Cell Culture , Serum-free and Speciality Media

Authors:

Tengku Ibrahim Maulana , Claudia Teufel, Madalena Cipriano , Julia Roosz , Lisa Lazarevski , Francijna E van den Hil , Lukas Scheller , Valeria Orlova , André Koch , Michael Hudecek , Miriam Alb , Peter Loskill

In:

Cell Stem Cell (2024) 31: 7

Human iPSC-derived liver co-culture spheroids to model liver fibrosis

Categories:

3D Cell Culture , Serum-free and Speciality Media

Authors:

Laura Cools , Mina Kazemzadeh Dastjerd , Ayla Smout , Vincent Merens , Yuwei Yang , Hendrik Reynaert , Nouredin Messaoudi , Vincent De Smet , Manoj Kumar , Stefaan Verhulst , Catherine Verfaillie , Leo A van Grunsven

In:

Biofabrication (2024) 16: 4

Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism

Categories:

Transfection

Authors:

Sébastien Levesque , Andrea Cosentino, Archana Verma , Pietro Genovese , Daniel E Bauer

In:

Nat Biotechnol (2024) :

Immunocompetent Intestine-on-Chip Model for Analyzing Gut Mucosal Immune Responses

Categories:

3D Cell Culture , Serum-free and Speciality Media

Authors:

Adrian Feile , Valentin D Wegner , Martin Raasch , Alexander S Mosig

In:

Journal of Visualized Experiments, JoVE (2024) 207:

Prediction of tumor-reactive T cell receptors from scRNA-seq data for personalized T cell therapy

Categories:

Transfection

Authors:

C L Tan , K Lindner, T Boschert , Z Meng , A Rodriguez Ehrenfried , A De Roia , G Haltenhof , A Faenza , F Imperatore, L Bunse , J M Lindner , R P Harbottle , M Ratliff , R Offringa, I Poschke , M Platten , E W Green

In:

Nature (2024) :

A simultaneous knockout knockin genome editingstrategy in HSPCs potently inhibits CCR5- andCXCR4-tropic HIV-1 infection

Categories:

Primary Cells and Media, Transfection

Authors:

Dudek AM, Feist WN, Sasu EJ, Luna SE, Ben-Efraim K, Bak RO, Cepika AM, Porteus MH

In:

Cell Stem Cell (2024) 31(4): 499-518.e6

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy

Categories:

Transfection

Authors:

Terheyden-Keighley D, Hühne M, Berger T, Hiller B, Martins S, Gamerschlag A, Sabour D, Meffert A, Kislat A, Slotta C, Hafezi F, Lichte J, Sudheer S, Tessmer K, Psathaki K, Ader M, Kogler G, Greber B.

In:

STEM CELLS TRANSLATIONALMEDICINE (2024) : szae047

Naturally occurring T cell mutations enhance engineered T cell therapies

Categories:

Serum-free and Speciality Media, Transfection

Authors:

Garcia J, Daniels J, Lee Y, Zhu I, Cheng K, Liu Q, Goodman D, Burnett C, Law C, Thienpont C, Alavi J, Azimi C, Montgomery G, Roybal KT, Choi J

In:

Nature (2024) 626(7999): 626-634

In vivo human T cell engineering with enveloped delivery vehicles

Categories:

Serum-free and Speciality Media, Transfection

Authors:

Hamilton JR, Chen E, Perez BS, Sandoval Espinoza CR, Kang MH, Trinidad M, Ngo W, Doudna JA

In:

Nature (2024) 11: 10.1038/s41587-023-02085

Modular Pooled Discovery of Synthetic Knockin Sequences to Program Durable Cell Therapies

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

F. Blaeschke, Y. Y. Chen, R. Apathy, Z. Li, C. T. Mowery, W.A. Nyberg, A. To, R. Yu, R. Bueno, M. C. Kim, R. Schmidt, D. B. Goodman, T. Feuchtinger, J. Eyquem, C. J. Ye, E. Shifrut, T. L. Roth, A. Marson

In:

Cell Res (2023) 186(19): :4216-4234.e33

Therapeutic adenine base editing of human hematopoietic stem cells

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Liao J, Chen S, Hsiao S, Jiang Y, Yang Y, Zhang Y, Wang X, Lai Y, Bauer DE, Wu Y

In:

Nat Commun. (2023) 14(1): 207

Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells

Authors:

Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC

In:

Nat Biomed Eng (2023) 5: 647-660

Mitigation of chromosome loss in clinical CRISPR Cas9-engineered T cells

Categories:

Primary Cells and Media, Transfection

Authors:

Tsuchida CA, Brandes N, Bueno R, Trinidad M, Mazumder T, Yu B, Hwang B, Chang C, Liu J, Sun Y, Hopkins CR, Parker KR, Qi Y, Satpathy AT, Stadtmauer EA, Cate JHD, Eyquem J, Fraietta JA, June CH, Chang HY, Ye CJ, Doudna JA

In:

Cell Rep (2023) 03.22: 533709

Automated production of gene-modified chimeric antigen receptor T cells using the Cocoon Platform

Categories:

Primary Cells and Media, Transfection, Endotoxin Detection

Authors:

Trainor N, Purpura KA, Middleton K, Fargo K, Hails L, Vicentini-Hogan M, McRobie C, Daniels R, Densham P, Gardin P, Fouks M, Brayer H, Malka RG, Rodin A, Ogen T, Besser MJ, Smith T, Leonard D, Bryan A.

In:

Cytotherapy (2023) 12: 1349-1360

An evolved AAV variant enables efficient genetic engineering of murine T cells

Categories:

Primary Cells and Media, Transfection

Authors:

Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, Xie WH, Allain V, Steinhart Z, Chang C, Talbot A, Kim S, Rosales A, Havlik LP, Pimentel H, Asokan A, Eyquem J

In:

Cell (2023) 186(2): 446-460

CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells

Categories:

Primary Cells and Media, Transfection

Authors:

Lin-Shiao E, Pfeifer WG, Shy BR, Saffari Doost M, Chen E, Vykunta VS, Hamilton JR, Stahl EC, Lopez DM, Sandoval Espinoza CR, Deyanov AE, Lew RJ, Poirer MG, Marson A, Castro CE, Doudna JA

In:

Nucleic Acids Res (2022) 50(3): 1256-1268

Pharmacological interventions enhance virus-free generation of TRAC-replaced CAR T cells

Categories:

Transfection

Authors:

Kath J, Du W, Pruene A, Braun T, Thommandru B, Turk R, Sturgeon ML, Kurgan GL, Amini L, Stein M, Zittel T, Martini S, Ostendorf L, Wilhelm A, Akyüz L, Rehm A, Höpken UE, Pruß A, Künkele A, Jacobi AM, Volk HD, Schmueck-Henneresse M, Stripecke R, Reinke P, Wagner DL

In:

Mol Ther Methods Clin Dev. (2022) 25: 311-330

NUDT21 limits CD19 levels through alternativemRNA polyadenylation in B cell acutelymphoblastic leukemia

Categories:

Primary Cells and Media, Transfection

Authors:

Witkowski MT, Lee S, Wang E, Lee AK, Talbot A, Ma C, Tsopoulidis N, Brumbaugh J, Zhao Y, Roberts KG, Hogg SJ, Nomikou S, Ghebrechristos YE, Thandapani P, Mullighan CG, Hochedlinger K, Chen W, Abdel-Wahab O, Eyquem J, Aifantis I

In:

Nature (2022) 10: 1424-1432

A reference human induced pluripotent stem cell line for large-scale collaborative studies

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, Santiana M, Faghri F, Nalls MA, Vitale D, Ballard S, Qi YA, Ramos DM, Anderson KM, Stadler J, Narayan P, Papademetriou J, Reilly L, Nelson MP, Aggarwal S, Rosen LU, Kirwan P, Pisupati V, Coon SL, Scholz SW, Priebe T, Öttl M, Dong J, Meijer M, Janssen LJM, Lourenco VS, van der Kant R, Crusius D, Paquet D, Raulin AC, Bu G, Held A, Wainger BJ, Gabriele RMC, Casey JM, Wray S, Abu-Bonsrah D, Parish CL, Beccari MS, Cleveland DW, Li E, Rose IVL, Kampmann M, Calatayud Aristoy C, Verstreken P, Heinrich L, Chen MY, Schüle B, Dou D, Holzbaur ELF, Zanellati MC, Basundra R, Deshmukh M, Cohen S, Khanna R, Raman M, Nevin ZS, Matia M, Van Lent J, Timmerman V, Conklin BR, Johnson Chase K, Zhang K, Funes S, Bosco DA, Erlebach L, Welzer M, Kronenberg-Versteeg D, Lyu G, Arenas E, Coccia E, Sarrafha L, Ahfeldt T, Marioni JC, Skarnes WC, Cookson MR, Ward ME, Merkle FT

In:

Cell Stem Cell (2022) 29(12): 1685-1702.e22

Fast and Efficient Genome Editing of Human FOXP3+ Regulatory T Cells

Categories:

Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells

Authors:

Van Zeebroeck L, Arroyo Hornero R, Côrte-Real BF, Hamad I, Meissner TB, Kleinewietfeld M.

In:

Frontiers in Immunology (2021) 12: 655122

CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cells

Categories:

Primary Cells and Media, Transfection

Authors:

Goodwin M, Lee E, Lakshmanan U, Shipp S, Froessl L, Barzaghi F, Passerini L, Narula M, Sheikali A, Lee CM, Bao G, Bauer CS, Miller HK, Garcia-Lloret M, Butte MJ, Bertaina A, Shah A, Pavel-Dinu M, Hendel A, Porteus M, Roncarolo MG, Bacchetta R.

In:

Science (2020) 6(19): eaaz0571

CRISPR/Cas9-Based Gene Engineering of Human Natural Killer Cells: Protocols for Knockout and Readouts to Evaluate Their Efficacy

Categories:

Serum-free and Speciality Media, Transfection

Authors:

Mélanie Lambert , Caroline Leijonhufvud , Filip Segerberg , J Joseph Melenhorst , Mattias Carlsten

In:

Methods Mol Biol (2020) 2121: 213-219

Successfully Transfected Primary Peripherally Mobilized Human CD34+ Hematopoietic Stem and Progenitor Cells (HSPCs) Demonstrate Increased Susceptibility to Retroviral Infection

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Jeffrey Sebrow , Stephen P Goff, Daniel O Griffin

In:

Virol J (2020) 17: 1

TGF-ß inhibition via CRISPR promotes the long-term efficacy of CAR T cells against solid tumors

Categories:

Transfection

Authors:

Tang N, Cheng C, Zhang X, Qiao M, Li N, Mu W, Wei XF, Han W, Wang H.

In:

(2020) 27;5(4): e133977

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

Categories:

Transfection

Authors:

Wienert B, Nguyen DN, Guenther A, Feng SJ, Locke MN, Wyman SK, Shin J, Kazane KR, Gregory GL, Carter MAM, Wright F, Conklin BR, Marson A, Richardson CD, Corn JE

In:

Nat Commun. (2020) 11(1): 2109

Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies

Categories:

Transfection

Authors:

Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A

In:

Cell Res (2020) 181(3): 728-744.e21

Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency.

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Nguyen DN, Roth TL, Li PJ, Chen PA, Apathy R, Mamedov MR, Vo LT, Tobin VR, Goodman D, Shifrut E, Bluestone JA, Puck JM, Szoka FC, Marson A.

In:

Nat Biotechnol (2019) 1: 1

Gene correction for SCID-X1 in long-term hematopoietic stem cells.

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Pavel-Dinu M, Wiebking V, Dejene BT, Srifa W, Mantri S, Nicolas CE, Lee C, Bao G, Kildebeck EJ, Punjya N, Sindhu C, Inlay MA, Saxena N, DeRavin SS, Malech H, Roncarolo MG, Weinberg KI, Porteus MH

In:

Nat Commun. (2019) 10 (1): 1634

Highly efficient therapeutic gene editing of human hematopoietic stem cells

Categories:

Serum-free and Speciality Media, Transfection

Authors:

Wu Y, Zeng J, Roscoe BP, Liu P, Yao Q, Lazzarotto CR, Clement K, Cole MA, Luk K, Baricordi C, Shen AH, Ren C, Esrick EB, Manis JP, Dorfman DM, Williams DA, Biffi A, Brugnara C, Biasco L, Brendel C, Pinello L, Tsai SQ, Wolfe SA, Bauer DE.

In:

Nat Med (2019) 25(5): 776-783

Immune tolerance in multiple sclerosis and neuromyelitis optica with peptide-loaded tolerogenic dendritic cells in a phase 1b trial.

Categories:

Serum-free and Speciality Media

Authors:

Zubizarreta I, Flórez-Grau G, Vila G, Cabezón R, España C, Andorra M, Saiz A, Llufriu S, Sepulveda M, Sola-Valls N, Martinez-Lapiscina EH, Pulido-Valdeolivas I, Casanova B, Martinez Gines M, Tellez N, Oreja-Guevara C, Español M, Trias E, Cid J, Juan M, Lozano M, Blanco Y, Steinman L, Benitez-Ribas D, Villoslada P

In:

Proc Natl Acad Sci USA (2019) 116(17): 8463-8470

Bacteria-free minicircle DNA system to generate integration-free CAR-T cells

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells

Authors:

Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang

In:

J Med Genetics (2019) 56: 10–17

Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function.

Categories:

Transfection

Authors:

Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME, Ashworth A, Marson A.

In:

Cell (2018) 175(7): 1985-1971

Cytokines induced killer cells produced in good manufacturing practices conditions: identification of the most advantageous and safest expansion method in terms of viability, cellular growth and identity.

Categories:

Serum-free and Speciality Media

Authors:

Castiglia S, Adamini A, Rustichelli D, Castello L, Mareschi K, Pinnetta G, Leone M, Mandese A, Ferrero I, Mesiano G, Fagioli F.

In:

J Transl Med (2018) 16: 237

A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Vakulskas CA, Dever DP, Rettig GR, Turk R, Jacobi AM, Collingwood MA, Bode NM, McNeill MS, Yan S, Camarena J, Lee CM, Park SH, Wiebking V, Bak RO, Gomez-Ospina N, Pavel-Dinu M, Sun W6, Bao G, Porteus MH, Behlke MA

In:

Nat Med (2018) 24(8): 1216-1224

Clinical grade manufacturing of genetically modified, CAR-expressing NK-92 cells for the treatment of ErbB2-positive malignancies.

Categories:

Serum-free and Speciality Media

Authors:

Nowakowska P, Romanski A, Miller N, Odendahl M, Bonig H, Zhang C, Seifried E, Wels WS, Tonn T

In:

Cancer Immunol Immunother (2018) 67 (1): 25-38

Improved Expansion and In Vivo Function of Patient T Cells by a Serum-free Medium.

Categories:

Serum-free and Speciality Media

Authors:

Medvec AR, Ecker C, Kong H, Winters EA, Glover J, Varela-Rohena A, Riley JL.

In:

Mol Ther Methods Clin Dev. (2017) 7; 8: 65-74

CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors.

Categories:

Transfection

Authors:

Bak RO, Porteus MH.

In:

Cell Rep (2017) 20(3): 750-756

Long-Term Engraftment and Fetal Globin Induction upon BCL11A Gene Editing in Bone-Marrow-Derived CD34+ Hematopoietic Stem and Progenitor Cells

Categories:

Primary Cells and Media, Serum-free and Speciality Media

Authors:

Chang KH, Smith SE, Sullivan T, Chen K, Zhou Q, West JA, Liu M, Liu Y, Vieira BF, Sun C, Hong VP, Zhang M, Yang X, Reik A2, Urnov FD, Rebar EJ, Holmes MC, Danos O, Jiang H, Tan S

In:

Mol Ther Methods Clin Dev. (2017) 4: 137-148

A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, Piechocka-Trocha , Krogan NJ, Marson A, Sabatini DM, Lander ES, Hacohen N, Walker BD.

In:

Nat Genet (2017) 49(2): 193-203

An optimized multiplex flow cytometry protocol for the analysis of intracellular signaling in peripheral blood mononuclear cells.

Categories:

Serum-free and Speciality Media

Authors:

Davies R, Vogelsang P, Jonsson R, Appel S.

In:

J Immunol Methods (2016) 436: 58-63

Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integration

Categories:

Serum-free and Speciality Media

Authors:

Griffin DO, Goff SP

In:

Retrovirology (2016) 13: 14

Type 1 diabetes immunotherapy using polyclonal regulatory T cells.

Categories:

Primary Cells and Media, Serum-free and Speciality Media

Authors:

Bluestone JA1, Buckner JH2, Fitch M3, Gitelman SE4, Gupta S2, Hellerstein MK3, Herold KC5, Lares A6, Lee MR6, Li K7, Liu W6, Long SA2, Masiello LM6, Nguyen V8, Putnam AL6, Rieck M6, Sayre PH9, Tang Q8.

In:

Med. Sci. (2015) 7: 315

Use of Genome Engineering to Create Patient Specific MLL Translocations in Primary Human Hematopoietic Stem and Progenitor Cells.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Breese EH, Buechele C, Dawson C, Cleary ML, Porteus MH.

In:

PLoS ONE (2015) 10(9: e0136644

Pathogenesis of ELANE-mutant severe neutropenia revealed by induced pluripotent stem cells

Categories:

Transfection

Authors:

Nayak RC, Trump LR, Aronow BJ, Myers K, Mehta P, Kalfa T, Wellendorf AM, Valencia CA, Paddison PJ, Horwitz MS, Grimes HL, Lutzko C, Cancelas JA

In:

J Clin Invest (2015) 125(8): 3103-16

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn, Matthew H Porteus

In:

Nat Biotechnol (2015) 10.1038: nbt.3290

Selective inhibition of tumor growth by clonal NK cells expressing an ErbB2/HER2-specific chimeric antigen receptor.

Categories:

Serum-free and Speciality Media

Authors:

Schönfeld K, Sahm C, Zhang C, Naundorf S, Brendel C, Odendahl M, Nowakowska P, Bönig H, Köhl U, Kloess S, Köhler S, Holtgreve-Grez H, Jauch A, Schmidt M, Schubert R, Kühlcke K, Seifried E, Klingemann HG, Rieger MA, Tonn T, Grez M, Wels WS.

In:

Mol Ther (2015) 23 (2): 330-8

Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promote

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Cooper AR, Lill GR, Gschweng EH, Kohn DB

In:

Nucleic Acids Res (2015) 43(1): 682-90

A novel method using blinatumomab for efficient, clinical-grade expansion of polyclonal T cells for adoptive immunotherapy.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Golay J, D'Amico A, Borleri G, Bonzi M, Valgardsdottir R, Alzani R, Cribioli S, Albanese C, Pesenti E, Finazzi MC, Quaresmini G, Nagorsen D, Introna M, Rambaldi A.

In:

J Immunol (2014) 139(9): 4739-47

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Maeß MB, Wittig B, Lorkowski S

In:

J Vis Exp (2014) 91: 1-9

Heparin disrupts the CXCR4/SDF-1 axis and impairs the functional capacity of bone marrow-derived mononuclear cells used for cardiovascular repair.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Seeger FH, Rasper T, Fischer A, Muhly-Reinholz M, Hergenreider E, Leistner DM, Sommer K, Manavski Y, Henschler R, Chavakis E, Assmus B, Zeiher AM, Dimmeler S

In:

Circ Res (2014) 111(7): 854-862

Dendritic cell-mediated, DNA-based vaccination against hepatitis C induces the multi-epitope-specific response of humanized, HLA transgenic mice.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Mishra S1, Lavelle BJ1, Desrosiers J2, Ardito MT3, Terry F3, Martin WD3, De Groot AS4, Gregory SH1

In:

PLoS ONE (2014) 9 (2): e87210

Interferon regulatory factor (IRF) 3 is critical for the development of experimental autoimmune encephalomyelitis

Categories:

Serum-free and Speciality Media

Authors:

Fitzgerald DC, O'Brien K, Young A, Fonseca-Kelly Z, Rostami A, Gran B.

In:

Journal of Neuroinflammation (2014) 11: 130

T-cell responses to oncogenic merkel cell polyomavirus proteins distinguish patients with merkel cell carcinoma from healthy donors.

Categories:

Serum-free and Speciality Media

Authors:

Lyngaa R1, Pedersen NW, Schrama D, Thrue CA, Ibrani D, Met O, Thor Straten P, Nghiem P, Becker JC, Hadrup SR.

In:

Clin Cancer Res (2014) 20(7): 1768-78

Human embryonic epidermis contains a diverse Langerhans cell precursor pool.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Schuster C1, Mildner M, Mairhofer M, Bauer W, Fiala C, Prior M, Eppel W, Kolbus A, Tschachler E, Stingl G, Elbe-Bürger A.

In:

Development (2014) 141(4): 807-15

Comparison of angiogenic, cytoprotective, and immunosuppressive properties of human amnion- and chorion-derived mesenchymal stem cells

Categories:

Authors:

Yamahara K, Harada K, Ohshima M, Ishikane S, Ohnishi S, Tsuda H, Otani K, Taguchi A, Soma T, Ogawa H, Katsuragi S, Yoshimatsu J, Harada-Shiba M, Kangawa K, Ikeda T

In:

PLoS ONE (2014) 9(2): e88319

Alloreactive cytotoxic T cells provide means to decipher the immunopeptidome and reveal a plethora of tumor-associated self-epitopes.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Kumari S1, Wälchli S, Fallang LE, Yang W, Lund-Johansen F, Schumacher TN, Olweus J.

In:

Proc Natl Acad Sci USA (2014) 111(1): 403-8

Cbl-b mediates TGFß sensitivity by downregulating inhibitory SMAD7 in primary T cells.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Gruber T1, Hinterleitner R, Hermann-Kleiter N, Meisel M, Kleiter I, Wang CM, Viola A, Pfeifhofer-Obermair C, Baier G.

In:

J Mol Biol (2013) 5(6): 358-68

Phagocytosis of photoreceptor outer segments by transplanted human neural stem cells as a neuroprotective mechanism in retinal degeneration.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Cuenca N1, Fernández-Sánchez L, McGill TJ, Lu B, Wang S, Lund R, Huhn S, Capela A.

In:

Invest Ophthalmol Vis Sci (2013) 54(10): 6745-56

Myeloblastic cell lines mimic some but not all aspects of human cytomegalovirus experimental latency defined in primary CD34+ cell populations.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Albright ER1, Kalejta RF

In:

J Virol (2013) 87(17): 9802-12

Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells

Categories:

Authors:

Rossetto CC, Tarrant-Elorza M, Pari GS

In:

PLoS Pathog (2013) 09(5): e1003366

Differential effects of rapamycin and retinoic acid on expansion, stability and suppressive qualities of human CD4(+)CD25(+)FOXP3(+) T regulatory cell subpopulations.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Scottà C1, Esposito M, Fazekasova H, Fanelli G, Edozie FC, Ali N, Xiao F, Peakman M, Afzali B, Sagoo P, Lechler RI, Lombardi G.

In:

Haematologica (2013) 98(8): 1291-9

Haemophilus ducreyi infection induces activation of the NLRP3 inflammasome in nonpolarized but not in polarized human macrophages.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Li W1, Katz BP, Bauer ME, Spinola SM.

In:

Infect Immun (2013) 81(8): 2997-3008

Wiskott-Aldrich syndrome protein-deficient hematopoietic cells can be efficiently mobilized by granulocyte colony-stimulating factor.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Charrier S1, Blundell M, Cédrone G, Louache F, Vainchenker W, Thrasher AJ, Galy A.

In:

Haematologica (2013) 98(8): 1300-8

Subretinal implantation of retinal pigment epithelial cells derived from human embryonic stem cells: improved survival when implanted as a monolayer.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Diniz B1, Thomas P, Thomas B, Ribeiro R, Hu Y, Brant R, Ahuja A, Zhu D, Liu L, Koss M, Maia M, Chader G, Hinton DR, Humayun MS.

In:

Invest Ophthalmol Vis Sci (2013) 54(7): 5087-96

Comparison of Lentiviral and Sleeping Beauty Mediated alpha beta T Cell Receptor Gene Transfer

Categories:

Transfection

Authors:

Anne-Christine Field, Conrad Vink, Richard Gabriel, Roua Al-Subki, Manfred Schmidt, Nicholas Goulden, Hans Stauss, Adrian Thrasher, Emma Morris, Waseem Qasim

In:

PLoS ONE (2013) 8(6): e68201

Interferon-gamma impairs proliferation of hematopoietic stem cells in mice.

Categories:

Serum-free and Speciality Media

Authors:

de Bruin AM, Demirel Ö, Hooibrink B, Brandts CH, Nolte MA.

In:

Blood (2013) 121(18): 3578-85

Peptide library-based evaluation of T-cell receptor breadth detects defects in global and regulatory activation in human immunologic diseases.

Categories:

Serum-free and Speciality Media

Authors:

Barber JS1, Yokomizo LK, Sheikh V, Freeman AF, Garabedian E, van Dijk E, Sokolic R, Candotti F, Weng NP, Sereti I, Milner JD.

In:

Proc Natl Acad Sci USA (2013) 110(20): 8164-9

Transgene-free iPSCs generated from small volume peripheral blood nonmobilized CD34+ cells.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Merling RK1, Sweeney CL, Choi U, De Ravin SS, Myers TG, Otaizo-Carrasquero F, Pan J, Linton G, Chen L, Koontz S, Theobald NL, Malech HL.

In:

Blood (2013) 121(14): e98-107

Fast dendritic cells stimulated with alternative maturation mixtures induce polyfunctional and long-lasting activation of innate and adaptive effector cells with tumor-killing capabilities.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Massa C1, Seliger B.

In:

J Immunol (2013) 190(7): 3328-37

Phase 2 clinical trial of rapamycin-resistant donor CD4+ Th2/Th1 (T-Rapa) cells after low-intensity allogeneic hematopoietic cell transplantation.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Fowler DH1, Mossoba ME, Steinberg SM, Halverson DC, Stroncek D, Khuu HM, Hakim FT, Castiello L, Sabatino M, Leitman SF, Mariotti J, Gea-Banacloche JC, Sportes C, Hardy NM, Hickstein DD, Pavletic SZ, Rowley S, Goy A, Donato M, Korngold R, Pecora A, Levine BL, June CH, Gress RE, Bishop MR.

In:

Blood (2013) 121(15): 2864-74

The myeloid transcription factor GATA-2 regulates the viral UL144 gene during human cytomegalovirus latency in an isolate-specific manner.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Poole E, Walther A, Raven K, Benedict CA, Mason GM, Sinclair J.

In:

J Virol (2013) 87(8): 4261-71

Potent endothelial progenitor cell-conditioned media-related anti-apoptotic, cardiotrophic, and pro-angiogenic effects post-myocardial infarction are mediated by insulin-like growth factor-1.

Categories:

Serum-free and Speciality Media

Authors:

Hynes B1, Kumar AH, O'Sullivan J, Klein Buneker C, Leblond AL, Weiss S, Schmeckpeper J, Martin K, Caplice NM.

In:

Eur J Cardiothorac Surg (2013) 34(10): 782-9

Engineering a BCR-ABL-activated caspase for the selective elimination of leukemic cells.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Kurokawa M1, Ito T, Yang CS, Zhao C, Macintyre AN, Rizzieri DA, Rathmell JC, Deininger MW, Reya T, Kornbluth S.

In:

Proc Natl Acad Sci USA (2013) 110(6): 2300-5

Experimental gingivitis induces systemic inflammatory markers in young healthy individuals: a single-subject interventional study.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Eberhard J, Grote K, Luchtefeld M, Heuer W, Schuett H, Divchev D, Scherer R, Schmitz-Streit R, Langfeldt D, Stumpp N, Staufenbiel I, Schieffer B, Stiesch M.

In:

PLoS ONE (2013) 8(2): e55265

Isolation of human CD4/CD8 double-positive, graft-versus-host disease-protective, minor histocompatibility antigen-specific regulatory T cells and of a novel HLA-DR7-restricted HY-specific CD4 clone.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Eljaafari A1, Yuruker O, Ferrand C, Farre A, Addey C, Tartelin ML, Thomas X, Tiberghien P, Simpson E, Rigal D, Scott D.

In:

J Immunol (2013) 190(1): 184-94

SB1578, a novel inhibitor of JAK2, FLT3, and c-Fms for the treatment of rheumatoid arthritis.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Madan B1, Goh KC, Hart S, William AD, Jayaraman R, Ethirajulu K, Dymock BW, Wood JM.

In:

J Immunol (2012) 189(8): 4123-34

Cutting edge: suppression of GM-CSF expression in murine and human T cells by IL-27.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Young A1, Linehan E, Hams E, O'Hara Hall AC, McClurg A, Johnston JA, Hunter CA, Fallon PG, Fitzgerald DC

In:

J Immunol (2012) 189 (5): 2079-83

Context-specific roles for paracrine IL-6 in lymphomagenesis.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Gilbert LA1, Hemann MT.

In:

Genes Dev (2012) 26(15): 1758-68

Cross talk between engulfment receptors stabilin-2 and integrin avß5 orchestrates engulfment of phosphatidylserine-exposed erythrocytes.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Kim S1, Park SY, Kim SY, Bae DJ, Pyo JH, Hong M, Kim IS.

In:

Mol Cell Biol (2012) 32(14): 2698-708

Nanoparticle-mediated codelivery of myelin antigen and a tolerogenic small molecule suppresses experimental autoimmune encephalomyelitis.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Yeste A1, Nadeau M, Burns EJ, Weiner HL, Quintana FJ.

In:

Proc Natl Acad Sci USA (2012) 109(28): 11270-5

Identification of early gene expression changes during human Th17 cell differentiation.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Tuomela S1, Salo V, Tripathi SK, Chen Z, Laurila K, Gupta B, Äijö T, Oikari L, Stockinger B, Lähdesmäki H, Lahesmaa R.

In:

Blood (2012) 119(23): e151-60

Xenografted islet cell clusters from INSLEA29Y transgenic pigs rescue diabetes and prevent immune rejection in humanized mice.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Klymiuk N1, van Buerck L, Bähr A, Offers M, Kessler B, Wuensch A, Kurome M, Thormann M, Lochner K, Nagashima H, Herbach N, Wanke R, Seissler J, Wolf E.

In:

Diabetes (2012) 61(6): 1527-32

Extensive determination of glycan heterogeneity reveals an unusual abundance of high mannose glycans in enriched plasma membranes of human embryonic stem cells.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

An HJ1, Gip P, Kim J, Wu S, Park KW, McVaugh CT, Schaffer DV, Bertozzi CR, Lebrilla CB.

In:

Mol Cell Proteomics (2012) 11(4): M111.010660

Increased expression of SLAM receptors SLAMF3 and SLAMF6 in systemic lupus erythematosus T lymphocytes promotes Th17 differentiation.

Categories:

Serum-free and Speciality Media

Authors:

Chatterjee M1, Rauen T, Kis-Toth K, Kyttaris VC, Hedrich CM, Terhorst C, Tsokos GC.

In:

J Immunol (2012) 188(3): 1206-12

Characterization and clinical evaluation of CD10+ stroma cells in the breast cancer microenvironment.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Desmedt C1, Majjaj S, Kheddoumi N, Singhal SK, Haibe-Kains B, El Ouriaghli F, Chaboteaux C, Michiels S, Lallemand F, Journe F, Duvillier H, Loi S, Quackenbush J, Dekoninck S, Blanpain C, Lagneaux L, Houhou N, Delorenzi M, Larsimont D, Piccart M, Sotiriou C.

In:

Clin Cancer Res (2012) 18(4): 1004-14

Saftey and persistence of adoptively transferred autologous CD19-targeted T cells in patients with relapsed or chemotherapy refractory B-cell leukemias

Categories:

Serum-free and Speciality Media

Authors:

Renier J. Brentjens, Isabelle Rivière, Jae H. Park, Marco L. Davila, Xiuyan Wang, Jolanta Stefanski, Clare Taylor, Raymond Yeh, Shirley Bartido, Oriana Borquez-Ojeda, Malgorzata Olszewska, Yvette Bernal, Hollie Pegram, Mark Przybylowski, Daniel Hollyman, Yelena Usachenko, Domenick Pirraglia, James Hosey, Elmer Santos, Elizabeth Halton, Peter Maslak, David Scheinberg, Joseph Jurcic, Mark Heaney, Glenn Heller, Mark Frattini, Michel Sadelain

In:

Blood (2011) 118 (18): 4817-4828

Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays

Categories:

Serum-free and Speciality Media, Transfection

Authors:

Hook L1, Vives J, Fulton N, Leveridge M, Lingard S, Bootman MD, Falk A, Pollard SM, Allsopp TE, Dalma-Weiszhausz D, Tsukamoto A, Uchida N, Gorba T.

In:

Neurochemistry International (2011) 59 (2011): 432-444

Notch signaling regulates mouse and human Th17 differentiation

Categories:

Authors:

Keerthivasan S1, Suleiman R, Lawlor R, Roderick J, Bates T, Minter L, Anguita J, Juncadella I, Nickoloff BJ, Le Poole IC, Miele L, Osborne BA

In:

J Immunol (2011) 187(2): 692-701

Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34? progenitors.

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Poole E, McGregor Dallas SR, Colston J, Joseph RS, Sinclair J

In:

J Gen Virol (2011) 92(7): 1539-49

Pregnancy-specific glycoprotein 1 induces endothelial tubulogenesis through interaction with cell surface proteoglycans

Categories:

Serum-free and Speciality Media

Authors:

Lisboa FA, Warren J, Sulkowski G, Aparicio M, David G, Zudaire E, Dveksler GS

In:

J Biol Chem (2011) 286(9): 7577-86

Human natural killer cells exhibit direct activity against Aspergillus fumigatus hyphae, but not against resting conidia

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Schmidt S1, Tramsen L, Hanisch M, Latgé JP, Huenecke S, Koehl U, Lehrnbecher T.

In:

J Infect Dis (2011) 203(3): 430-5

Human hematopoietic stem/progenitor cells modified by zinc-finger nucleases targeted to CCR5 control HIV-1 in vivo

Categories:

Transfection

Authors:

Holt N, Wang J, Kim K, Friedman G, Wang X, Taupin V, Crooks GM, Kohn DB, Gregory PD, Holmes MC, Cannon PM.

In:

Nat Biotechnol (2010) 28: 839-847

NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A.

In:

Blood (2008) 111(3): 1428-36

Generation and maturation of bone marrow-derived DCs under serum-free conditions

Categories:

Serum-free and Speciality Media

Authors:

Sung Jung Kim, Betty Diamond

In:

J Immunol Methods (2007) 323: 2007

Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function

Categories:

Serum-free and Speciality Media, Transfection

Authors:

Kubach J, Lutter P, Bopp T, Stoll S, Becker C, Huter E, Richter C, Weingarten P, Warger T, Knop J, Mullner S, Wijdenes J, Schild H, Schmitt E, Jonuleit H

In:

Blood (2007) 110(5): 1550-8

CD4 +CD25 + regulatory T cells decreased the antitumor activity of cytokine-induced killer (CIK) cells of lung cancer patients.

Categories:

Serum-free and Speciality Media

Authors:

Li H1, Yu JP, Cao S, Wei F, Zhang P, An XM, Huang ZT, Ren XB.

In:

J Clin Immunol (2007) 27 (3): 317-26

IL-2/B7.1 (CD80) fusagene transduction of AML blasts by a self-inactivating lentiviral vector stimulates T cell responses in vitro: a strategy to generate whole cell vaccines for AML.

Categories:

Serum-free and Speciality Media

Authors:

Chan L, Hardwick N, Darling D, Galea-Lauri J, Gäken J, Devereux S, Kemeny M, Mufti G, Farzaneh F.

In:

Mol Ther (2005) 11(1): 120-131

Adoptive transfer of costimulated T cells induces lymphocytosis in patients with relapsed/refractory non-Hodgkin lymphoma following CD34+-selected hematopoietic cell transplantation.

Categories:

Serum-free and Speciality Media

Authors:

Laport GG, Levine BL, Stadtmauer EA, Schuster SJ, Luger SM, Grupp S, Bunin N, Strobl FJ, Cotte J, Zheng Z, Gregson B, Rivers P, Vonderheide RH, Liebowitz DN, Porter DL, June CH.

In:

Blood (2003) 102 (6): 2004-13

Flt3-mediated signaling in human acute myelogenous leukemia (AML) blasts: a functional characterization of Flt3-ligand effects in AML cell populations with and without genetic Flt3 abnormalities.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Bruserud Ø, Hovland R, Wergeland L, Huang TS, Gjertsen BT.

In:

Haematologica (2003) 88(4): 416-28

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