Monocyte, human

Monocytes are found in the circulating peripheral blood where they make up 10-20% of the mononuclear cells. They play an important role in host defense, both as circulating monocytes and by leaving the circulation and differentiating to tissue macrophages. Monocytes also differentiate into dendritic cells with potent antigen-presenting capability, in vivo and in vitro. Macrophages themselves are difficult to isolate from tissue due to their phenotypic diversity in various tissues. Consequently, monocytes, which are the precursors to tissue macrophages, are a convenient source to use for macrophage research.
Macrophages play key roles in cellular immunity, via phagocytosis and other signaling mechanisms, in addition to tissue remodeling that is essential to development and repair.
Monocytes can be isolated from peripheral blood using the cell surface marker CD14. CD14, the LPS receptor, is expressed on most monocytes, but not on other mononuclear cells in the blood. A second cell surface marker found on most monocytes is CD11b, a subunit of the integrin family.

Cell Type:
Monocyte
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Adherent

Recommended Media

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and human or recombinant transferrin.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 EA-100 5e6 51-77% 67-87% Plasmid (general) 2 µg 100 µl 4D X-Unit
P3

EA-100

1e6 51-77% 67-87% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
Monocyte, human Y-001 3e6 42-70% 71-91% Plasmid (general) 1 µg 100 µl I/II/2b
Monocyte, human Y-001 3e6 34-76% 49-75% Plasmid (general) 1 µg 100 µl I/II/2b
P3 96-EA-100 1e6 51-77% 67-87% Plasmid (general) 0.4 µg 20 µl Shuttle

Citations (51)

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