The myeloid transcription factor GATA-2 regulates the viral UL144 gene during human cytomegalovirus latency in an isolate-specific manner.

Poole E, Walther A, Raven K, Benedict CA, Mason GM, Sinclair J.
Source: J Virol
Publication Date: (2013)
Issue: 87(8): 4261-71
Research Area:
Stem Cells
Basic Research
Cells used in publication:
CD34+ cell, human
Species: human
Tissue Origin: blood
Monocyte, human
Species: human
Tissue Origin: blood
PBMC, human
Species: human
Tissue Origin: blood
Culture Media:
It is generally accepted that, following primary infection, human cytomegalovirus (HCMV) establishes lifelong latency in CD34(+) progenitor cells and other derivative cells of the myeloid lineage. In this study, we show that the viral UL144 gene is expressed during latent infection in two cell types of the myeloid lineage, CD34(+) and CD14(+) monocytes, and that the UL144 protein is functional in latently infected monocytes. However, this latency-associated expression of UL144 occurs only in certain isolates of HCMV and depends on the presence of functional GATA-2 transcription factor binding sites in the UL144 promoter, in contrast to the viral latency-associated gene LUNA, which we also show is regulated by GATA-2 but expressed uniformly during latent infection independent of the virus isolate. Taken together, these data suggest that the HCMV latency-associated transcriptome may be virus isolate specific and dependent on the repertoire of transcription factor binding sites in the promoters of latency-associated genes