PBMC, human

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Primary cells isolated from peripheral blood.

Cell Type:
PBMC
Tissue Origin:
blood
Species:
human
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Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

Lymphocyte Growth Medium-3
LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T cell, human U-014 1e6 50% Plasmid (general) µg 100 µl I/II/2b
T cell, human U-017 5e5 50% 50% Plasmid (general) µg 100 µl I/II/2b

Citations (62)

Hepatitis Delta Virus Acts as an Immunogenic Adjuvant in Hepatitis B Virus-Infected Hepatocytes
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Christine Y.L. Tham, Janine Kah, Anthony T. Tan, ..., Marc L€utgehetmann, Maura Dandri, Antonio Bertoletti 
In:
Cell Rep (2020) 1 (4): 100060 
CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell Therapy
Categories:
Transfection 
Authors:
Zelda Odé , Jose Condori , Nicolas Peterson , Sheng Zhou , Giedre Krenciute  
In:
Cancers (2020) 12(6): 1704 
Establishment of induced pluripotent stem cell line CSUASOi004-A by reprogramming peripheral blood mononuclear cells of a PRPF6-related dominant retinitis pigmentosa patient
Categories:
Transfection 
Authors:
Yalan Zhou, Yutong Jing, Shengru Mao, Jian Liu, Zekai Cui, Yini Wang, Juan Chen, Hon Fai Chan, Shibo Tang, Jiansu Chen 
In:
Stem Cell Res (2020) 45: 101793 
An integration-free iPSC line (SDQLCHi017-A) derived from a patient with nemaline myopathy-2 disease carrying compound heterozygote mutations in NEB gene
Categories:
Transfection 
Authors:
Yanyan Ma, Haiyan Zhang, Xiaomei Li, Xiaomeng Yang, Yue Li, Jingyun Guan, Yuqiang Lv, Zhongtao Gai, Yi Liu 
In:
Stem Cell Res (2020) 43: 101729 
Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune-Oncology Studies Evaluating New Therapeutic Agents
Categories:
Primary Cells and Media 
Authors:
Bhavna Verma , Amy Wesa  
In:
Curr Protoc Immunol (2020) 89: 1 
Reprogramming of human Peripheral Blood Mononuclear Cell (PBMC) from a Chinese patient suffering Duchenne muscular dystrophy to iPSC line (SDQLCHi007-A) carrying deletion of 49-50 exons in the DMD gene
Categories:
Transfection 
Authors:
Jingyun Guan, Xinnong Liu, Haiyan Zhang, Xiaomeng Yang, Yanyan Ma, Yue Li, Zhongtao Gai, Yi Liu 
In:
Stem Cell Res (2020) 42: 101666 
Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
Categories:
Transfection 
Authors:
Paredes BD, Martins GLS, Azevedo CM, Sampaio GLA, Nonaka CKV, Silva KND, Soares MBP, Santos RRD, Souza BSF 
In:
Stem Cell Res (2019) 38: 101454 
Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function.
Categories:
Transfection 
Authors:
Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME,  Ashworth A, Marson A.  
In:
Cell (2018) 175(7): 1985-1971 
Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR
Categories:
Primary Cells and Media, Transfection 
Authors:
Hamada M1, Nishio N2, Okuno Y3, Suzuki S3, Kawashima N1, Muramatsu H1, Tsubota S4, Wilson MH5, Morita D6, Kataoka S1, Ichikawa D1, Murakami N1, Taniguchi R1, Suzuki K1, Kojima D1, Sekiya Y1, Nishikawa E1, Narita A1, Hama A1, Kojima S1, Nakazawa Y6, Takahashi Y7. 
In:
EBioMedicine (2018) 2352:  
Nucleofection with Plasmid DNA for CRISPR/Cas9-Mediated Inactivation of Programmed Cell Death Protein 1 in CD133-Specific CAR T Cells
Categories:
Transfection 
Authors:
Hu B, Zou Y, Zhang L, Tang J, Niedermann G, Firat E, Huang X, Zhu X.  
In:
Hum Gene Ther (2018) :  
Generation of integration-free iPS cell lines from three sickle cell disease patients from the state of Bahia, Brazil
Categories:
Transfection 
Authors:
Martins GLS, Paredes BD, Azevedo CM, Sampaio GLA, Nonaka CKV, Cavalcante BRR, Da Silva KN, Pereira CSE, Soares MBP, Dos Santos RR, Souza BSF 
In:
Stem Cell Res (2018) 33: 10-14 
Quantitative analysis of the CD4+ T cell response to therapeutic antibodies in healthy donors using a novel T cell:PBMC assay.
Categories:
Primary Cells and Media 
Authors:
Schultz HS1, Reedtz-Runge SL2, Bäckström BT1, Lamberth K1, Pedersen CR3, Kvarnhammar AM1; ABIRISK consortium. 
In:
PLoS ONE (2017) 12: 5 
Anti-proliferative effects of T cells expressing a ligand-based chimeric antigen receptor against CD116 on CD34(+) cells of juvenile myelomonocytic leukemia.
Categories:
Transfection 
Authors:
Nakazawa Y, Matsuda K, Kurata T, Sueki A, Tanaka M, Sakashita K, Imai C, Wilson MH, Koike K 
In:
J Hematol Oncol (2016) 9 (1): 1-11 
IGF1R- and ROR1-Specific CAR T Cells as a Potential Therapy for High Risk Sarcomas
Categories:
Transfection 
Authors:
Xin Huang, Haein Park, Joseph Greene, James Pao, Erin Mulvey, Sophia X. Zhou, Catherine M. Albert, Fred Moy, Deepali Sachdev, Douglas Yee, Christoph Rader, Carl V. Hamby, David M. Loeb, Mitchell S. Cairo, Xianzheng Zhou 
In:
PLoS ONE (2015) 10(7): e0133152 
Sleeping Beauty Transposition of Chimeric Antigen Receptors Targeting Receptor Tyrosine Kinase-Like Orphan Receptor-1 (ROR1) into Diverse Memory T-Cell Populations.
Categories:
Transfection 
Authors:
Deniger DC, Yu J, Huls MH, Figliola MJ, Mi T, Maiti SN, Widhopf GF 2nd, Hurton LV, Thokala R, Singh H3, Olivares S, Champlin RE, Wierda WG, Kipps TJ, Cooper LJ. 
In:
PLoS Genet (2015) 10(6): e0128151 
A facile method to establish human induced pluripotent stem cells from adult blood cells under feeder-free and xeno-free culture conditions: a clinically compliant approach
Categories:
Transfection 
Authors:
Chou BK, Gu H, Gao Y, Dowey SN, Wang Y, Shi J, Li Y, Ye Z, Cheng T, Cheng L 
In:
STEM CELLS TRANSLATIONALMEDICINE (2015) 4: 320-332 
Pharmacological properties of JTE-052: a novel potent JAK inhibitor that suppresses various inflammatory responses in vitro and in vivo.
Categories:
Primary Cells and Media 
Authors:
Tanimoto A, Ogawa Y, Oki C, Kimoto Y, Nozawa K, Amano W, Noji S, Shiozaki M, Matsuo A, Shinozaki Y, Matsushita M 
In:
Other (2015) 64(1): 41-51 
Characterization of the CD14++CD16+ monocyte population in human bone marrow.
Categories:
Primary Cells and Media 
Authors:
Mandl M, Schmitz S, Weber C, Hristov M 
In:
PLoS ONE (2014) 9(11): ePub 
Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Marie Klinge Brimnes, Bjarke Endel Hansen, Leif Kofoed Nielsen, Morten Hanefeld Dziegiel, Claus Henrik Nielsen 
In:
PLoS ONE (2014) 17(9): e113388 
CD4 T cells require ICOS-mediated PI3K signaling to increase T-Bet expression in the setting of anti-CTLA-4 therapy.
Categories:
Transfection 
Authors:
Chen H1, Fu T, Suh WK, Tsavachidou D, Wen S, Gao J, Ng Tang D, He Q, Sun J, Sharma P. 
In:
Cancer Immunol Immunother (2014) 2(2): 167-76. 
Efficient generation of integration-free ips cells from human adult peripheral blood using BCL-XL together with Yamanaka factors.
Categories:
Transfection 
Authors:
Su RJ, Baylink DJ, Neises A, Kiroyan JB, Meng X, Payne KJ, Tschudy-Seney B, Duan Y, Appleby N, Kearns-Jonker M, Gridley DS, Wang J, Lau KH, Zhang XB. 
In:
PLoS ONE (2013) 8(5): 1-10 
The myeloid transcription factor GATA-2 regulates the viral UL144 gene during human cytomegalovirus latency in an isolate-specific manner.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Poole E, Walther A, Raven K, Benedict CA, Mason GM, Sinclair J. 
In:
J Virol (2013) 87(8): 4261-71 
A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR.
Categories:
Transfection 
Authors:
Torikai H, Reik A, Liu PQ, Zhou Y, Zhang L, Maiti S, Huls H, Miller JC, Kebriaei P, Rabinovitch B, Lee DA, Champlin RE, Bonini C, Naldini L, Rebar EJ, Gregory PD, Holmes MC, Cooper LJ. 
In:
Blood (2012) 119(24): 5697-5705 
An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.
Categories:
 
Authors:
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S. 
In:
Stem Cells (2012) 31(3): 458-466 
Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors
Categories:
Transfection 
Authors:
Mack AA, Kroboth S, Rajesh D, Wang WB 
In:
PLoS ONE (2011) 6 (11): e27956 
Human leukocyte antigen I knockdown human embryonic stem cells induce host ignorance and achieve prolonged xenogeneic survival
Categories:
Primary Cells and Media 
Authors:
Deuse T, Seifert M, Phillips N, Fire A, Tyan D, Kay M, Tsao PS, Hua X, Velden J, Eiermann T, Volk HD, Reichenspurner H, Robbins RC, Schrepfer S. 
In:
Circulation (2011) 124(11): ePub 
Genetic analysis of SH2D4A, a novel adapter protein related to T cell-specific adapter and adapter protein in lymphocytes of unknown function, reveals a redundant function in T cells
Categories:
Transfection 
Authors:
Lapinski PE, Oliver JA, Kamen LA, Hughes ED, Saunders TL, King PD 
In:
J Immunol (2008) 181(3): 2019-27 
Galectin-4 controls intestinal inflammation by selective regulation of peripheral and mucosal T cell apoptosis and cell cycle
Categories:
Transfection 
Authors:
Paclik D, Danese S, Berndt U, Wiedenmann B, Dignass A, Sturm A 
In:
PLoS ONE (2008) 3(7): e2629 
Active beta-catenin signaling is an inhibitory pathway of HIV replication in peripheral blood mononuclear cells
Categories:
Transfection 
Authors:
Kumar A, Zloza A, Moon RT, Watts J, Tenorio AR, Al-Harthi L 
In:
J Virol (2008) 82(6): 2813-20 
Protein protein interaction inhibition (2P2I) combining high throughput and virtual screening: Application to the HIV-1 Nef protein
Categories:
Transfection 
Authors:
Betzi S, Restouin A, Opi S, Arold ST, Parrot I, Guerlesquin F, Morelli X, Collette Y 
In:
Proc Natl Acad Sci USA (2007) 104(49): 19256-61 
Zap70 signaling pathway mediates glucocorticoid receptor-dependent transcriptional activation: role in the regulation of annexin 1 expression in T cells
Categories:
Transfection 
Authors:
Ishaq M, Degray G, Mou K, Aguilera A, Yang J, Lempicki RA, Hazen A, Natarajan V 
In:
J Immunol (2007) 179(6): 3851-8 
Apoptosis-inducing factor regulates death in peripheral T cells
Categories:
Transfection 
Authors:
Srivastava S, Banerjee H, Chaudhry A, Khare A, Sarin A, George A, Bal V, Durdik JM, Rath S 
In:
J Immunol (2007) 179(2): 797-803 
Innate immunity and transcription of MGAT-III and Toll-like receptors in Alzheimer's disease patients are improved by bisdemethoxycurcumin
Categories:
Transfection 
Authors:
Fiala M, Liu PT, Espinosa-Jeffrey A, Rosenthal MJ, Bernard G, Ringman JM, Sayre J, Zhang L, Zaghi J, Dejbakhsh S, Chiang B, Hui J, Mahanian M, Baghaee A, Hong P, Cashman J 
In:
Proc Natl Acad Sci USA (2007) 104(31): 12849-54 
Production of Specific mRNA Transcripts, Usage of an Alternate Promoter, and Octamer-Binding Transcription Factors Influence the Surface Expression Levels of the HIV Coreceptor CCR5 on Primary T Cells
Categories:
Transfection 
Authors:
Mummidi S, Adams LM, Vancompernolle SE, Kalkonde M, Camargo JF, Kulkarni H, Bellinger AS, Bonello G, Tagoh H, Ahuja SS, Unutmaz D, Ahuja SK 
In:
J Immunol (2007) 178(9): 5668-81 
Suppression of microRNA-silencing pathway by HIV-1 during virus replication
Categories:
Transfection 
Authors:
Triboulet R, Mari B, Lin YL, Chable-Bessia C, Bennasser Y, Lebrigand K, Cardinaud B, Maurin T, Barbry P, Baillat V, Reynes J, Corbeau P, Jeang KT, Benkirane M 
In:
Science (2007) 315(5818): 1579-82 
Full activation of the T cell receptor requires both clustering and conformational changes at CD3
Categories:
Transfection 
Authors:
Minguet S, Swamy M, Alarcon B, Luescher IF, Schamel WW 
In:
Immunity (2007) 26(1): 43-54 
Filamin A is required for T cell activation mediated by protein kinase C-theta
Categories:
Transfection 
Authors:
Hayashi K, Altman A 
In:
J Immunol (2006) 177(3): 1721-8 
Induction of Lactoferrin Gene Expression in Myeloid or Mammary Gland Cells by Human T-Cell Leukemia Virus Type 1 (HTLV-1) Tax: Implications for Milk-Borne Transmission of HTLV-1
Categories:
Transfection 
Authors:
Moriuchi M, Moriuchi H 
In:
J Virol (2006) 80(14): 7118-26 
HIV-1 GP120-mediated apoptosis of T cells is regulated by the membrane tyrosine phosphatase, CD45
Categories:
Transfection 
Authors:
Anand AR, Ganju RK 
In:
J Biol Chem (2006) 281(18): 12289-99 
Loss of Siglec expression on T lymphocytes during human evolution
Categories:
Transfection 
Authors:
Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A 
In:
Proc Natl Acad Sci USA (2006) 103(20): 7765-70 
Transduction of Primary Lymphocytes with Epstein-Barr Virus (EBV) Latent Membrane Protein-Specific T-Cell Receptor Induces Lysis of Virus-Infected Cells: A Novel Strategy for the Treatment of Hodgkin's Disease and Nasopharyngeal Carcinoma
Categories:
Transfection 
Authors:
Jurgens LA, Khanna R, Weber J, Orentas RJ 
In:
J Clin Immunol (2006) 26(1): 22-32 
Active repression of IFN regulatory factor-1-mediated transactivation by IFN regulatory factor-4
Categories:
Transfection 
Authors:
Yoshida K, Yamamoto K, Kohno T, Hironaka N, Yasui K, Kojima C, Mukae H, Kadota JI, Suzuki S, Honma K, Kohno S and Matsuyama T 
In:
Int Immunol (2005) 17(11): 1463-1471 
The Cyclic AMP Response Element Modulator Regulates Transcription of the TCR -Chain
Categories:
Transfection 
Authors:
Tenbrock K, Kyttaris VC, Ahlmann M, Ehrchen JM, Tolnay M, Melkonyan H, Mawrin C, Roth J, Sorg C, Juang YT and Tsokos GC 
In:
J Immunol (2005) 175(9): 5975-5980 
Fluorescent antigen-transfected target cell cytotoxic T lymphocyte assay for ex vivo detection of antigen-specific cell-mediated cytotoxicity
Categories:
Transfection 
Authors:
van Baalen CA, Kwa D, Verschuren EJ, Reedijk ML, Boon AC, de Mutsert G, Rimmelzwaan GF, Osterhaus AD and Gruters RA 
In:
J Infect Dis (2005) 192(7): 1183-1190 
CTLA-4 up-regulation of lymphocyte function- associated antigen 1 adhesion and clustering as an alternate basis for coreceptor function
Categories:
Transfection 
Authors:
Schneider H, Valk E, da Rocha Dias S, Wei B and Rudd CE 
In:
Proc Natl Acad Sci USA (2005) 102(36): 12861-12866 
Distinct Transcriptional Control Mechanisms of Killer Immunoglobulin-like Receptors in Natural Killer (NK) and in T Cells
Categories:
Transfection 
Authors:
Xu J, Vallejo AN, Jiang Y, Weyand CM and Goronzy JJ 
In:
J Biol Chem (2005) 280(25): 24277-24285 
Control of lymphocyte shape and the chemotactic response by the GTP exchange factor Vav
Categories:
Transfection 
Authors:
Vicente-Manzanares M, Cruz-Adalia A, Martin-Cofreces NB, Cabrero JR, Dosil M, Alvarado-Sanchez B, Bustelo XR, Sanchez-Madrid F 
In:
Blood (2005) 105(8): 3026-3034 
WHIM syndromes with different genetic anomalies are accounted for by impaired CXCR4 desensitization to CXCL12
Categories:
Transfection 
Authors:
Balabanian K, Lagane B, Pablos JL, Laurent L, Planchenault T, Verola O, Lebbe C, Kerob D, Dupuy A, Hermine O, Nicolas JF, Latger-Cannard V, Bensoussan D, Bordigoni P, Baleux F, Le Deist F, Virelizier JL, Arenzana-Seisdedos F and Bachelerie F 
In:
Blood (2005) 105(6): 2449-2457 
CD4 downregulation by HIV-1 and SIV Nef proteins involves both internalization and intracellular retention mechanisms
Categories:
Transfection 
Authors:
Rose JJ, Janvier K, Chandrasekhar S, Sekaly RP, Bonifacino JS and Venkatesan S 
In:
J Biol Chem (2005) 280(9): 7413-7426 
c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription
Categories:
Transfection 
Authors:
Arabi A, Wu S, Ridderstrale K, Bierhoff H, Shiue C, Fatyol K, Fahlen S, Hydbring P, Soderberg O, Grummt I, Larsson LG, Wright APArabi A, Wu S, Ridderstrale K, Bierhoff H, Shiue C, Fatyol K, Fahlen S, Hydbring P, Soderberg O, Grummt I, Larsson LG and Wright AP 
In:
Nat Cell Biol (2005) 7(3): 303-310 
Rodent cells support key functions of the human immunodeficiency virus type 1 pathogenicity factor Nef.
Categories:
Transfection 
Authors:
Keppler OT, Allespach I, Schuller L, Fenard D, Greene WC and Fackler OT 
In:
J Virol (2005) 79(3): 1655-1665 
Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton
Categories:
Transfection 
Authors:
Barda-Saad M, Braiman A, Titerence R, Bunnell SC, Barr VA and Samelson LE 
In:
Nat Immunol (2005) 6(1): 80-89 
IL-2/B7.1 (CD80) fusagene transduction of AML blasts by a self-inactivating lentiviral vector stimulates T cell responses in vitro: a strategy to generate whole cell vaccines for AML.
Categories:
Serum-free and Speciality Media 
Authors:
Chan L, Hardwick N, Darling D, Galea-Lauri J, Gäken J, Devereux S, Kemeny M, Mufti G, Farzaneh F. 
In:
Mol Ther (2005) 11(1): 120-131 
Interleukin (IL)-15 and IL-2 Reciprocally Regulate Expression of the Chemokine Receptor CX3CR1 through Selective NFAT1- and NFAT2-dependent Mechanisms
Categories:
Transfection 
Authors:
Barlic J, McDermott DH, Merrell MN, Gonzales J, Via LE and Murphy PM 
In:
J Biol Chem (2004) 279(47): 48520-48534 
Seminal fluid enhances replication of human T-cell leukemia virus type 1: implications for sexual transmission
Categories:
Transfection 
Authors:
Moriuchi M and Moriuchi H 
In:
J Virol (2004) 78(22): 12709-12711 
GA binding protein regulates interleukin 7 receptor alpha-chain gene expression in T cells
Categories:
Transfection 
Authors:
Xue HH, Bollenbacher J, Rovella V, Tripuraneni R, Du YB, Liu CY, Williams A, McCoy JP and Leonard WJ 
In:
Nat Immunol (2004) 5(10): 1036-1044 
Engineering RENTA, a DNA prime-MVA boost HIV vaccine tailored for Eastern and Central Africa
Categories:
Transfection 
Authors:
Nkolola JP, Wee EG, Im EJ, Jewell CP, Chen N, Xu XN, McMichael AJ and Hanke T 
In:
Gene Ther (2004) 11(13): 1068-1080 
Conversion of Bcl-2 from protector to killer by interaction with nuclear orphan receptor Nur77/TR3
Categories:
Transfection 
Authors:
Lin B, Kolluri SK, Lin F, Liu W, Han YH, Cao X, Dawson MI, Reed JC and Zhang XK 
In:
Cell (2004) 116(4): 527-540 
T-cell receptor/CD28 engagement when combined with prostaglandin E2 treatment leads to potent activation of human T-cell leukemia virus type 1
Categories:
Transfection 
Authors:
Dumais N, Pare ME, Mercier S, Bounou S, Marriot SJ, Barbeau B and Tremblay MJ 
In:
J Virol (2003) 77(20): 11170-11179 
Detection of a gene cluster that is dispensable for human herpesvirus 6 replication and latency
Categories:
Transfection 
Authors:
Kondo K, Nozaki H, Shimada K and Yamanishi K 
In:
J Virol (2003) 77(19): 10719-10724 
Characterization and analysis of the proximal Janus Kinase 3 promoter
Categories:
Transfection 
Authors:
Aringer M, Hofmann S, Frucht DM, Chen M, Centola M, Morinobu A, Visconti R, Kastner DL, Smolen JS and O'Shea JJ 
In:
J Immunol (2003) 170(12): 6057-6064 
YY1 transcription factor down-regulates expression of CCR5, a major co-receptor for HIV-1
Categories:
Transfection 
Authors:
Moriuchi M and Moriuchi H 
In:
J Biol Chem (2003) 278(15): 13003-13007 
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