Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies

Authors:
Paredes BD, Martins GLS, Azevedo CM, Sampaio GLA, Nonaka CKV, Silva KND, Soares MBP, Santos RRD, Souza BSF
In:
Source: Stem Cell Res
Publication Date: (2019)
Issue: 38: 101454
Research Area:
Immunotherapy / Hematology
Stem Cells
Cells used in publication:
PBMC, human
Species: human
Tissue Origin: blood
Induced Pluripotent Stem Cell (iPS), human
Species: human
Tissue Origin:
Platform:
4D-Nucleofector™ X-Unit
Experiment

Authors used 4D Nucleofector to reprogram erythroblasts into iPS. Episomal vectors from Addgene were nucleofected using P3 Solution and pulse EO-100. 

Abstract

Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders. Previously, we generated iPS cell lines from SCD patients. Here, we generated
iPS cell lines from three age-, ethnicity- and gender-matched healthy individuals as control cell lines. Cell reprogramming was performed using erythroblasts
expanded from PBMC by a non-integrative method. SCD-iPSC controls expressed pluripotency markers, presented a normal karyotype, were able to differentiate into
the three germ layers in embryoid body spontaneous differentiation and confirmed to be integration-free. The cell lines generated here may be used as matched
healthy controls for SCD studies.