Induced Pluripotent Stem Cell (iPS), human

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Pluripotent stem cell artificially derived from a non-pluripotent cell, typically an adult somatic cell, by inducing a forced expression of certain genes.

Cell Type:
Misc. Somatic SC
Tissue Origin:
Species:
human
Research Area:
Dermatology/Tissue Engineering
Stem Cells
Basic Research
Regenerative medicine
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform


2e5 50-75% 50-75% Plasmid (general) 0.4 µg 20 µl 96-well


2e5 50-75% 50-75% Plasmid (general) 0.4 µg 20 µl 4D X-Unit


8e5 50-60% 50-75% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (69)

Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways
Categories:
Transfection 
Authors:
Schimmel J, Muñoz-Subirana N, Kool H, van Schendel R, van der Vlies S, Kamp JA, de Vrij FMS, Kushner SA, Smith GCM, Boulton SJ, Tijsterman M. 
In:
Cell Rep (2023) 42(2): 112019 
Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells
Categories:
Transfection 
Authors:
Wu Y, Sidharta M, Zhong A, Persily B, Li M, Zhou T 
In:
STAR Protoc (2023) 4(4): 102583 
Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells
Categories:
Primary Cells and Media 
Authors:
Rong Li , Yang Zhao , Jeffrey J Yourick , Robert L Sprando , Xiugong Gao  
In:
Biomed Res Int. (2022) 16: 43 
Generation of Complex Syngeneic Liver Organoids from Induced Pluripotent Stem Cells to Model Human Liver Pathophysiology
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Claudia Raggi , Silvia Selleri , Marie-Agnes M'Callum , Massimiliano Paganelli  
In:
Curr Protoc Immunol (2022) 2: e389 
A reference human induced pluripotent stem cell line for large-scale collaborative studies
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, Santiana M, Faghri F, Nalls MA, Vitale D, Ballard S, Qi YA, Ramos DM, Anderson KM, Stadler J, Narayan P, Papademetriou J, Reilly L, Nelson MP, Aggarwal S, Rosen LU, Kirwan P, Pisupati V, Coon SL, Scholz SW, Priebe T, Öttl M, Dong J, Meijer M, Janssen LJM, Lourenco VS, van der Kant R, Crusius D, Paquet D, Raulin AC, Bu G, Held A, Wainger BJ, Gabriele RMC, Casey JM, Wray S, Abu-Bonsrah D, Parish CL, Beccari MS, Cleveland DW, Li E, Rose IVL, Kampmann M, Calatayud Aristoy C, Verstreken P, Heinrich L, Chen MY, Schüle B, Dou D, Holzbaur ELF, Zanellati MC, Basundra R, Deshmukh M, Cohen S, Khanna R, Raman M, Nevin ZS, Matia M, Van Lent J, Timmerman V, Conklin BR, Johnson Chase K, Zhang K, Funes S, Bosco DA, Erlebach L, Welzer M, Kronenberg-Versteeg D, Lyu G, Arenas E, Coccia E, Sarrafha L, Ahfeldt T, Marioni JC, Skarnes WC, Cookson MR, Ward ME, Merkle FT 
In:
Cell Stem Cell (2022) 29(12): 1685-1702.e22 
Modeling retinitis pigmentosa through patient-derived retinal organoids
Categories:
Transfection 
Authors:
Yan-Ping Li , Wen-Li Deng , Zi-Bing Jin  
In:
STAR Protoc (2021) 2(2): 100438 
Phenotypic manifestation of a-synuclein strains derived from Parkinson's disease and multiple system atrophy in human dopaminergic neurons
Categories:
Transfection 
Authors:
Tanudjojo B, Shaikh SS, Fenyi A, Bousset L, Agarwal D, Marsh J, Zois C, Heman-Ackah S, Fischer R, Sims D, Melki R, Tofaris GK 
In:
Nat Commun. (2021) 12 (1): 3817 
Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells
Categories:
3D Cell Culture , Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Aditya Mithal, Amalia Capilla,Dar Heinze, Andrew Berical, Carlos Villacorta-Martin, Marall Vedaie, Anjali Jacob, Kristine Abo, Aleksander Szymaniak, Megan Peasley, Alexander Stuffer, John Mahoney, Darrell N. Kotton, Finn Hawkins, and Gustavo Mostoslavsky 
In:
Nat Commun. (2020) 11: 215 
Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Bin Ramli MN, Lim YS, Koe CT, Demircioglu D, Tng W, Uy Gonzales KA, Tan CP, Szczerbinska I, Liang H, Soe EL, Lu Z, Ariyachet C, Yu KM, Koh SH, Yaw LP, Binte Jumat NH, Yew Lim JS, Wright G, Shabbir A, Dan YY, Ng H-H, Chan Y-S 
In:
World J Gastroenterol (2020) 159(4): 1471-1486 
Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells
Categories:
Primary Cells and Media, 3D Cell Culture , Serum-free and Speciality Media, Transfection 
Authors:
Kazuki Takeishi, Alexandra Collin de l’Hortet, Yang Wang, Kan Handa, Jorge Guzman-Lepe, Kentaro Matsubara, Kazutoyo Morita, Sae Jang, Nils Haep, Rodrigo M. Florentino, Fangchao Yuan, Ken Fukumitsu, Kimimasa Tobita, Wendell Sun, Jonatha Franks, Evan R. Delgado, Erik M. Shapiro, Nicolas A. Fraunhoffer, Andrew W. Duncan, Hiroshi Yagi, Tomoji Mashimo, Ira J. Fox, and Alejandro Soto-Gutierrez  
In:
Cell Rep (2020) 31(9): 107711 
Comparison of commercially available media for hepatic differentiation and hepatocyte maintenance
Categories:
Primary Cells and Media 
Authors:
Toba Y, Deguchi S, Mimura N, Sakamoto A, Harada K, Hirata K, Takayama K, Mizuguchi H. 
In:
PLoS ONE (2020) 15: e0229654  
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
Categories:
Transfection 
Authors:
Wienert B, Nguyen DN, Guenther A, Feng SJ, Locke MN, Wyman SK, Shin J, Kazane KR, Gregory GL, Carter MAM, Wright F, Conklin BR, Marson A, Richardson CD, Corn JE 
In:
Nat Commun. (2020) 11(1): 2109 
Generation of CCR5-ablated Human Induced Pluripotent Stem Cells as a Therapeutic Approach for Immune-mediated Diseases
Categories:
Transfection 
Authors:
Negin Hosseini Rouzbahani , Saeid Kaviani , Mohammad Vasei , Masoud Soleimani , Kayhan Azadmanesh , Mohammad Hossein Nicknam  
In:
Allergy Asthma Immunol Res. (2019) 18(3): 310-319 
Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders.
Categories:
Primary Cells and Media, Transfection 
Authors:
Sauter EJ, Kutsche LK, Klapper SD, Busskamp V. 
In:
Methods Mol Biol (2019) 1942: 101-121 
Human blood vessel organoids as a model of diabetic vasculopathy
Categories:
Primary Cells and Media, Transfection 
Authors:
Wimmer RA, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, Taubenschmid J, Hämmerle M, Esk C, Bagley JA, Lindenhofer D, Chen G, Boehm M, Agu CA, Yang F, Fu B, Zuber J, Knoblich JA, Kerjaschki D, Penninger JM. 
In:
Nature (2019) 565 (7740): 505-510 
Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
Categories:
Transfection 
Authors:
Paredes BD, Martins GLS, Azevedo CM, Sampaio GLA, Nonaka CKV, Silva KND, Soares MBP, Santos RRD, Souza BSF 
In:
Stem Cell Res (2019) 38: 101454 
Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells
Categories:
Transfection 
Authors:
Madsen RR, Semple RK 
In:
(2019) 11;4: 37 
Efficient CRISPR/Cas9-mediated editing of trinucleotide repeat expansion in myotonic dystrophy patient-derived iPS and myogenic cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Dastidar S, Ardui S, Singh K, Majumdar D, Nair N, Fu Y, Reyon D, Samara E, Gerli MFM, Klein AF, De Schrijver W, Tipanee J, Seneca S, Tulalamba W, Wang H, Chai YC, In't Veld P, Furling D, Tedesco FS, Vermeesch JR, Joung JK, Chuah MK, VandenDriessche T.  
In:
Nucleic Acids Res (2018) 46(16): 8275-8298 
Generation of integration-free iPS cell lines from three sickle cell disease patients from the state of Bahia, Brazil
Categories:
Transfection 
Authors:
Martins GLS, Paredes BD, Azevedo CM, Sampaio GLA, Nonaka CKV, Cavalcante BRR, Da Silva KN, Pereira CSE, Soares MBP, Dos Santos RR, Souza BSF 
In:
Stem Cell Res (2018) 33: 10-14 
FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson’s Disease Modeling
Categories:
Transfection 
Authors:
Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, Nickels SL, Zaehres H, Schöler HR, Schwamborn JC 
In:
Stem Cell Reports (2017) 14;9(5): 1423-1431 
CRISPR-Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice.
Categories:
Transfection 
Authors:
Zhang Y, Long C, Li H, McAnally JR, Baskin KK1,2,3, Shelton JM4, Bassel-Duby R, Olson EN, 
In:
Sci Adv. (2017) 12, 3(4): 1-11 
A Genome-wide Analysis of Human Pluripotent Stem Cell-Derived Endothelial Cells in 2D or 3D Culture.
Categories:
3D Cell Culture  
Authors:
Zhang J, Schwartz MP, Hou Z, Bai Y, Ardalani H, Swanson S, Steill J, Ruotti V, Elwell A, Nguyen BK, Bolin J, Stewart R, Thomson JA, Murphy WL 
In:
Stem Cell Reports (2017) 8(4): 907-918 
Generation of human pluripotent stem cell-derived hepatocyte-like cells for drug toxicity screening.
Categories:
3D Cell Culture  
Authors:
Takayama K, Mizuguchi H 
In:
Drug Metab Pharmacokinet (2017) 32(1): 12-20 
Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies.
Categories:
Transfection 
Authors:
Wang G, Yang L, Grishin D, Rios X, Ye LY, Hu Y, Li K, Zhang D, Church GM, Pu WT. 
In:
Nat Protocols (2017) 12(1): 88-103 
Rhesus iPSC Safe Harbor Gene-Editing Platform for Stable Expression of Transgenes in Differentiated Cells of All Germ Layers.
Categories:
Transfection 
Authors:
Hong SG, Yada RC, Choi K, Carpentier A, Liang TJ, Merling RK, Sweeney CL, Malech HL, Jung M, Corat MAF, AlJanahi AA, Lin Y, Liu H, Tunc I, Wang X, Palisoc M, Pittaluga S, Boehm M, Winkler T, Zou J, Dunbar CE. 
In:
Mol Ther (2017) 25(1): 44-53 
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chal J, Al Tanoury Z, Hestin M, Gobert B, Aivio S, Hick A, Cherrier T, Nesmith AP, Parker KK, Pourquié O 
In:
Nat Protocols (2016) 11(10): 1833-50 
Combining single-strand oligodeoxynucleotides and CRISPR/Cas9 to correct gene mutations in ß-thalassemia-induced Pluripotent Stem Cells
Categories:
Transfection 
Authors:
Xiaohua Niu, Wenyin He, Bing Song, Zhanhui Ou, Di Fan, Yuchang Chen, Yong Fan, Xiaofang Sun 
In:
J Biol Chem (2016) 291(32): 16576-85 
Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific homozygous mutation in CHMP2B.
Categories:
Transfection 
Authors:
Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, Freude KK. 
In:
Stem Cell Res (2016) 17(1): 151-153 
Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells.
Categories:
Primary Cells and Media 
Authors:
Peters DT, Henderson CA, Warren CR, Friesen M, Xia F, Becker CE, Musunuru K, Cowan CA 
In:
Development (2016) 143(9): 1475-1481 
A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells.
Categories:
Transfection 
Authors:
Young CS, Hicks MR, Ermolova NV, Nakano H, Jan M, Younesi S, Karumbayaram S, Kumagai-Cresse C, Wang D, Zack JA, Kohn DB, Nakano A, Nelson SF, Miceli MC, Spencer MJ, Pyle AD. 
In:
Cell Stem Cell (2016) 18(4): 533-40 
CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs
Categories:
Transfection 
Authors:
Mohammad A. Mandegar, Nathaniel Huebsch, Ekaterina B. Frolov, Edward Shin, Annie Truong, Michael P. Olvera, Amanda H. Chan, Yuichiro Miyaoka, Kristin Holmes, C. Ian Spencer, Luke M. Judge, David E. Gordon, Tilde V. Eskildsen, Jacqueline E. Villalta, Max A. Horlbeck, Luke A. Gilbert, Nevan J. Krogan, Søren P. Sheikh, Jonathan S. Weissman, Lei S. Qi, Po-Lin So, and Bruce R. Conklin 
In:
Cell Stem Cell (2016) 18 (4): 541-53 
Genome Editing of the CYP1A1 Locus in iPSCs as a Platform to Map AHR Expression throughout Human Development
Categories:
Transfection 
Authors:
BrendenW. Smith, Elizabeth A. Stanford, David H. Sherr, and George J.Murphy 
In:
Stem Cells Intl (2016) 2016: 1-11 
Optogenetic stimulation of multiwell MEA plates for neural and cardiac applications
Categories:
Primary Cells and Media 
Authors:
Clements IP, Millard DC, Nicolini AM, Preyer AJ, Grier R, Heckerling A, Blum RA, Tyler P, McSweeney KM, Lu Y,Hall D, Ross JD 
In:
Other (2016) 9690: ePub 
Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H196
Categories:
Transfection 
Authors:
Adele G. Marthaler, Benjamin Schmid, Alisa Tubsuwan, Ulla B. Poulsenc, Alexander F. Engelbrecht,Ulrike A. Mau-Holzmann , Poul Hyttel , Jørgen E. Nielsen, Troels T. Nielsenb, Bjørn Holst 
In:
Stem Cell Res (2016) 16: 162-165 
Generation of Naivetropic Induced Pluripotent Stem Cells from Parkinson's Disease Patients for High-Efficiency Genetic Manipulation and Disease Modeling.
Categories:
Transfection 
Authors:
Hu Z, Pu J, Jiang H, Zhong P, Qiu J, Li F, Wang X, Zhang B, Yan Z, Feng J1. 
In:
Stem Cells Dev (2015) 24(21): 2591-25604 
CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in neurodevelopment
Categories:
Transfection 
Authors:
Ping Wang, Mingyan Lin, Erika Pedrosa, Anastasia Hrabovsky, Zheng Zhang, Wenjun Guo, Herbert M. Lachman,and Deyou Zheng 
In:
Molecular Autism (2015) 6(55): 0.1186/s13229-015-0048-6 
Generation of Isogenic Human iPS Cell Line Precisely Corrected by Genome Editing Using the CRISPR/Cas9 System.
Categories:
Transfection 
Authors:
Grobarczyk B, Franco B, Hanon K, Malgrange B. 
In:
Stem Cells (2015) 11(5): 774-87 
Genomic DISC1 Disruption in hiPSCs Alters Wnt Signaling and Neural Cell Fate
Categories:
Transfection 
Authors:
Priya Srikanth, Karam Han, Dana G. Callahan, Eugenia Makovkina, Christina R. Muratore, Matthew A. Lalli, Honglin Zhou, Justin D. Boyd, Kenneth S. Kosik, Dennis J. Selkoe, Tracy L. Young-Pearse 
In:
Cell Rep (2015) 12: 1-16 
A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production.
Categories:
Transfection 
Authors:
Manzini S, Viiri LE, Marttila S, Aalto-Setälä K 
In:
Stem Cell Reports (2015) epub: 1-9 
Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.
Categories:
Transfection 
Authors:
Firth AL, Menon T, Parker GS, Qualls SJ, Lewis BM, Ke E, Dargitz CT, Wright R1, Khanna A, Gage FH, Verma IM 
In:
Cell Rep (2015) 12(9): 1385-1390 
Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
Categories:
Transfection 
Authors:
Raj Chari, Prashant Mali, Mark Moosburner, George M Church 
In:
Nat Methods (2015) 12(9): 823-6 
Pathogenesis of ELANE-mutant severe neutropenia revealed by induced pluripotent stem cells
Categories:
Transfection 
Authors:
Nayak RC, Trump LR, Aronow BJ, Myers K, Mehta P, Kalfa T, Wellendorf AM, Valencia CA, Paddison PJ, Horwitz MS, Grimes HL, Lutzko C, Cancelas JA 
In:
J Clin Invest (2015) 125(8): 3103-16 
Prediction of drug-induced nephrotoxicity and injury mechanisms with human induced pluripotent stem cell-derived cells and machine learning methods.
Categories:
Primary Cells and Media 
Authors:
Kandasamy K1, Chuah JK1, Su R2, Huang P1, Eng KG1, Xiong S1, Li Y1, Chia CS1, Loo LH3, Zink D1 
In:
Scientific Reports (2015) 27(5): 12337 
Rescue of DNA-PK Signaling and T-Cell Differentiation by Targeted Genome Editing in a prkdc Deficient iPSC Disease Model.
Categories:
Transfection 
Authors:
Rahman SH, Kuehle J, Reimann C, Mlambo T, Alzubi J, Maeder ML, Riedel H, Fisch P, Cantz T, Rudolph C, Mussolino C, Joung JK, Schambach A, Cathomen T 
In:
PLoS Genet (2015) 11(5): e1005239 
Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature.
Categories:
Transfection 
Authors:
Thomas SM, Kagan C, Pavlovic BJ, Burnett J, Patterson K, Pritchard JK, Gilad Y 
In:
PLoS Genet (2015) 11(5): e1005216 
Highly efficient Cas9-mediated transcriptional programming.
Categories:
Transfection 
Authors:
Chavez A1, Scheiman J2, Vora S3, Pruitt BW4, Tuttle M4, P R Iyer E2, Lin S5, Kiani S6, Guzman CD4, Wiegand DJ4, Ter-Ovanesyan D2, Braff JL4, Davidsohn N2, Housden BE7, Perrimon N7, Weiss R8, Aach J9, Collins JJ10, Church GM2. 
In:
Nat Methods (2015) 12(4): 326-8 
Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach
Categories:
Transfection 
Authors:
Debowski K, Warthemann R, Lentes J, Salinas-Riester G, Dressel R, Langenstroth D, Gromoll J, Sasaki E, Behr R. 
In:
PLoS ONE (2015) 10(3): 1-21 
A platform for rapid generation of single and multiplexed reporters in human iPSC lines
Categories:
Transfection 
Authors:
Pei Y, Sierra G, Sivapatham R, Swistowski A, Rao MS, Zeng X 
In:
Scientific Reports (2015) 5: 9205 
Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells
Categories:
Transfection 
Authors:
Susan M. Byrne, Luis Ortiz, Prashant Mali, John Aach, George M. Church 
In:
Nucleic Acids Res (2015) 43(3): e21 
Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines.
Categories:
Transfection 
Authors:
Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J. 
In:
PLoS ONE (2015) 10(1): 1-18 
Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells
Categories:
Transfection 
Authors:
Luhan Yang, Dennis Grishin, Gang Wang, John Aach, Cheng-Zhong Zhang, Raj Chari, Jason Homsy, Xuyu Cai, Yue Zhao, Jian-Bing Fan, Christine Seidman, Jonathan Seidman, William Pu, George Church 
In:
Nat Commun. (2014) 5: 5507 
Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs
Categories:
Transfection 
Authors:
Brian M Wile, Kiwon Ban, Young-Sup Yoon, Gang Bao 
In:
Nat Protocols (2014) 9(10): 2411-24 
Nuclease-mediated double-strand break (DSB) enhancement of small fragment homologous recombination (SFHR) gene modification in human-induced pluripotent stem cells (hiPSCs).
Categories:
Transfection 
Authors:
Sargent RG, Suzuki S, Gruenert DC. 
In:
Methods Mol Biol (2014) 1114: 279-90 
Stable enhanced green fluorescent protein expression after differentiation and transplantation of reporter human induced pluripotent stem cells generated by AAVS1 transcription activator-like effector nucleases.
Categories:
Transfection 
Authors:
Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao MS, Zou J. 
In:
STEM CELLS TRANSLATIONALMEDICINE (2014) 3(7): 821-35 
DICE, an efficient system for iterative genomic editing in human pluripotent stem cells.
Categories:
Transfection 
Authors:
Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schüle B, Chen-Tsai Y, Calos MP. 
In:
Proc Natl Acad Sci USA (2014) 42(5): e34 
Nanog Overexpression Increases Neural Marker Expression in Adipose Derived Stem Cells
Categories:
Primary Cells and Media 
Authors:
Kimmel J, Sugaya K 
In:
Other (2014) 4(1): 2210-2973 
Genome Editing in Human Stem Cells
Categories:
Transfection 
Authors:
Susan M. Byrne, Prashant Mali, and George M. Church 
In:
Methods Enzymol (2014) 546: 119-37 
Generation of Human Induced Pluripotent Stem Cells Using Epigenetic Regulators Reveals a Germ Cell-Like Identity in Partially Reprogrammed Colonies
Categories:
Transfection 
Authors:
Goyal A, Chavez SL, Reijo Pera RA. 
In:
PLoS ONE (2013) 8(12): e82838 
A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific induced pluripotent stem (iPS) cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Goh PA, Caxaria S, Casper C, Rosales C, Warner TT, Coffey PJ, Nathwani AC 
In:
PLoS ONE (2013) 8(11): e81622 
Optimization of scarless human stem cell genome editing.
Categories:
Transfection 
Authors:
Yang L, Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang PY, Daley G, Church G. 
In:
Nucleic Acids Res (2013) 41(19): 9049-61 
RNA-guided human genome engineering via Cas9
Categories:
Transfection 
Authors:
Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM 
In:
Science (2013) 339(6121): 823-826 
From skin biopsy to neurons through a pluripotent intermediate under Good Manufacturing Practice protocols
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Karumbayaram S, Lee P, Azghadi SF, Cooper AR, Patterson M, Kohn DB, Pyle A, Clark A, Byrne J, Zack JA, Plath K, Lowry WE 
In:
STEM CELLS TRANSLATIONALMEDICINE (2012) 1(1): 36-43 
Growth of engineered human myocardium with mechanical loading and vascular coculture.
Categories:
Primary Cells and Media 
Authors:
Tulloch NL, Muskheli V, Razumova MV, Korte FS, Regnier M, Hauch KD, Pabon L, Reinecke H, Murry CE. 
In:
Circ Res (2011) 109(1): 47-59 
Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders.
Categories:
Primary Cells and Media 
Authors:
Ye Z, Zhan H, Mali P, Dowey S, Williams DM, Jang YY, Dang CV, Spivak JL, Moliterno AR, Cheng L 
In:
Blood (2009) 114(27): 5473-80 
Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells.
Categories:
Transfection 
Authors:
Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L. 
In:
Stem Cells (2009) 5(1): 97-110 
Human induced pluripotent stem cells free of vector and transgene sequences.
Categories:
Transfection 
Authors:
Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. 
In:
Science (2009) 324(5928): 797-801 
Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media
Categories:
Primary Cells and Media, 3D Cell Culture , Serum-free and Speciality Media 
Authors:
Keisuke Sekine , Shimpei Ogawa, Syusaku Tsuzuki, Tatsuya Kobayashi , Kazuki Ikeda, Noriko Nakanishi , Kenta Takeuchi , Eriko Kanai , Yugo Otake , Satoshi Okamoto , Tsuyoshi Kobayashi , Takanori Takebe , Hideki Taniguchi  
In:
Scientific Reports () 10: 17937 
Standard operating protocol of hepatic organoid differentiation from human induced pluripotent stem cells 
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Min Jung Kim, Jaeseo Lee , Seon Ju Mun, Myung Jin Son, Jung-Hyun Kim 
In:
Other () 2: e5 
High temporal resolution proteome and phosphoproteome profiling of stem cell-derived hepatocyte development
Categories:
Primary Cells and Media, Serum-free and Speciality Media 
Authors:
Johannes Krumm, Keisuke Sekine , Patroklos Samaras, Agnieska Brazovskaja , Markus Breunig , Ryota Yasui , Alexander Kleger , Hideki Taniguchi , Mathias Wilhelm , Barbara Treutlein , J Gray Camp , Bernhard Kuster  
In:
Cell Rep () 38: 110604 
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