cells

Pluripotent stem cell artificially derived from a non-pluripotent cell, typically an adult somatic cell, by inducing a forced expression of certain genes.

Cell Type:

Misc. Somatic SC

Tissue Origin:

Species:

human

Research Area:

Dermatology/Tissue Engineering

Stem Cells

Basic Research

Regenerative medicine

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Cells	Efficiency	Viable Cells	Substrate		Format	Platform
2e5	50-75%	50-75%	Plasmid (general)	0.4 µg	20 µl	96-well
2e5	50-75%	50-75%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
8e5	50-60%	50-75%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (82)

An hepatitis B and D virus infection model using human pluripotent stem cell-derived hepatocytes

Categories:

Serum-free and Speciality Media

Authors:

Huanting Chi, Bingqian Qu, Angga Prawira, Talisa Richardt, Lars Maurer, Jungen Hu, Rebecca M Fu , Florian A Lempp, Zhenfeng Zhang , Dirk Grimm, Xianfang Wu, Stephan Urban, Viet Loan Dao Thi

In:

EMBO Rep (2024) 25: 10

Automated CRISPR/Cas9-based genome editing of human pluripotent stem cells using the StemCellFactory

Categories:

Transfection

Authors:

Bastian Nießing, Yannik Breitkreuz , Andreas Elanzew , Marcelo A S de Toledo , Peter Vajs , Marina Nolden , Frederik Erkens , Paul Wanek , Si Wah Christina Au Yeung , Simone Haupt , Niels König , Michael Peitz, Robert H Schmitt , Martin Zenke, Oliver Brüstle

In:

Front Bioeng Biotechnol (2024) 12: 1459273

A High-Throughput Microphysiological Liver Chip System to Model Drug-Induced Liver Injury Using Human Liver Organoids

Categories:

3D Cell Culture , Serum-free and Speciality Media

Authors:

Sophia R. Meyer , Charles J. Zhang , Max A. Garcia, Megan C. Procario , Sanghee Yoo , Amber L. Jolly, Sumin Kim , Jiho Kim , Kyusuk Baek , Roland D. Kersten , Robert J. Fontana , Jonathan Z. Sexton

In:

(2024) :

Long-term HBV infection of engineered cultures of induced pluripotent stem cell-derived hepatocytes

Categories:

Primary Cells and Media

Authors:

Yang Yuan , Vedant V Bodke , Christine Lin , Shang Gao , Jalees Rehman , Jisu Li , Salman R Khetani

In:

Hepatology (2024) 8: e0506

Modeling the Effects of Protracted Cosmic Radiation in a Human Organ-on-Chip Platform

Categories:

3D Cell Culture

Authors:

Daniel Naveed Tavakol , Trevor R Nash , Youngbin Kim Pamela L Graney , Martin Liberman , Sharon Fleischer , Roberta I Lock , Aaron O'Donnell , Leah Andrews , Derek Ning , Keith Yeager , Andrew Harken , Naresh Deoli , Sally A Amundson , Guy Garty , Kam W Leong , David J Brenner , Gordana Vunjak-Novakovic

In:

(2024) :

Human iPSC-derived liver co-culture spheroids to model liver fibrosis

Categories:

3D Cell Culture , Serum-free and Speciality Media

Authors:

Laura Cools , Mina Kazemzadeh Dastjerd , Ayla Smout , Vincent Merens , Yuwei Yang , Hendrik Reynaert , Nouredin Messaoudi , Vincent De Smet , Manoj Kumar , Stefaan Verhulst , Catherine Verfaillie , Leo A van Grunsven

In:

Biofabrication (2024) 16: 4

Hepatocyte-intrinsic SMN deficiency drives metabolic dysfunction and liver steatosis in spinal muscular atrophy

Categories:

Serum-free and Speciality Media

Authors:

Damien Meng-Kiat Leow , Yang Kai Ng , Loo Chien Wang , Hiromi Wl Koh , Tianyun Zhao , Zi Jian Khong , Tommaso Tabaglio , Gunaseelan Narayanan , Richard M Giadone , Radoslaw M Sobota , Shi-Yan Ng , Adrian Kk Teo , Simon H Parson , Lee L Rubin , Wei-Yi Ong , Basil T Darras , Crystal Jj Yeo

In:

(2024) : e173702

Human induced pluripotent stem cells-derived liver organoidsgrown on a Biomimesys®hyaluronic acid-based hydroscaffoldas a new model for studying human lipoprotein metabolism

Categories:

3D Cell Culture , Serum-free and Speciality Media

Authors:

In:

Medical & Bioengineering & Computing (2024) e10659:

GMP-compliant iPS cell lines show widespread plasticity in a new set of differentiation workflows for cell replacement and cancer immunotherapy

Categories:

Transfection

Authors:

Terheyden-Keighley D, Hühne M, Berger T, Hiller B, Martins S, Gamerschlag A, Sabour D, Meffert A, Kislat A, Slotta C, Hafezi F, Lichte J, Sudheer S, Tessmer K, Psathaki K, Ader M, Kogler G, Greber B.

In:

STEM CELLS TRANSLATIONALMEDICINE (2024) : szae047

Liver three-dimensional cellular models for high-throughput chemical testing

Categories:

3D Cell Culture

Authors:

Shu Yang , Masato Ooka , Ryan Jared Margolis , Menghang Xia

In:

Cell Rep (2023) 3: 100432

Modulating mutational outcomes and improving precise gene editing at CRISPR-Cas9-induced breaks by chemical inhibition of end-joining pathways

Categories:

Transfection

Authors:

Schimmel J, Muñoz-Subirana N, Kool H, van Schendel R, van der Vlies S, Kamp JA, de Vrij FMS, Kushner SA, Smith GCM, Boulton SJ, Tijsterman M.

In:

Cell Rep (2023) 42(2): 112019

Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells

Categories:

Transfection

Authors:

Wu Y, Sidharta M, Zhong A, Persily B, Li M, Zhou T

In:

STAR Protoc (2023) 4(4): 102583

Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells

Categories:

Primary Cells and Media

Authors:

Rong Li , Yang Zhao , Jeffrey J Yourick , Robert L Sprando , Xiugong Gao

In:

Biomed Res Int. (2022) 16: 43

Generation of Complex Syngeneic Liver Organoids from Induced Pluripotent Stem Cells to Model Human Liver Pathophysiology

Categories:

Primary Cells and Media, 3D Cell Culture

Authors:

Claudia Raggi , Silvia Selleri , Marie-Agnes M'Callum , Massimiliano Paganelli

In:

Curr Protoc Immunol (2022) 2: e389

A reference human induced pluripotent stem cell line for large-scale collaborative studies

Categories:

Primary Cells and Media, Serum-free and Speciality Media, Transfection

Authors:

Pantazis CB, Yang A, Lara E, McDonough JA, Blauwendraat C, Peng L, Oguro H, Kanaujiya J, Zou J, Sebesta D, Pratt G, Cross E, Blockwick J, Buxton P, Kinner-Bibeau L, Medura C, Tompkins C, Hughes S, Santiana M, Faghri F, Nalls MA, Vitale D, Ballard S, Qi YA, Ramos DM, Anderson KM, Stadler J, Narayan P, Papademetriou J, Reilly L, Nelson MP, Aggarwal S, Rosen LU, Kirwan P, Pisupati V, Coon SL, Scholz SW, Priebe T, Öttl M, Dong J, Meijer M, Janssen LJM, Lourenco VS, van der Kant R, Crusius D, Paquet D, Raulin AC, Bu G, Held A, Wainger BJ, Gabriele RMC, Casey JM, Wray S, Abu-Bonsrah D, Parish CL, Beccari MS, Cleveland DW, Li E, Rose IVL, Kampmann M, Calatayud Aristoy C, Verstreken P, Heinrich L, Chen MY, Schüle B, Dou D, Holzbaur ELF, Zanellati MC, Basundra R, Deshmukh M, Cohen S, Khanna R, Raman M, Nevin ZS, Matia M, Van Lent J, Timmerman V, Conklin BR, Johnson Chase K, Zhang K, Funes S, Bosco DA, Erlebach L, Welzer M, Kronenberg-Versteeg D, Lyu G, Arenas E, Coccia E, Sarrafha L, Ahfeldt T, Marioni JC, Skarnes WC, Cookson MR, Ward ME, Merkle FT

In:

Cell Stem Cell (2022) 29(12): 1685-1702.e22

Modeling retinitis pigmentosa through patient-derived retinal organoids

Categories:

Transfection

Authors:

Yan-Ping Li , Wen-Li Deng , Zi-Bing Jin

In:

STAR Protoc (2021) 2(2): 100438

Phenotypic manifestation of a-synuclein strains derived from Parkinson's disease and multiple system atrophy in human dopaminergic neurons

Categories:

Transfection

Authors:

Tanudjojo B, Shaikh SS, Fenyi A, Bousset L, Agarwal D, Marsh J, Zois C, Heman-Ackah S, Fischer R, Sims D, Melki R, Tofaris GK

In:

Nat Commun. (2021) 12 (1): 3817

Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells

Categories:

3D Cell Culture , Transfection, Cell Lines and Primary Cancer Cells

Authors:

Aditya Mithal, Amalia Capilla,Dar Heinze, Andrew Berical, Carlos Villacorta-Martin, Marall Vedaie, Anjali Jacob, Kristine Abo, Aleksander Szymaniak, Megan Peasley, Alexander Stuffer, John Mahoney, Darrell N. Kotton, Finn Hawkins, and Gustavo Mostoslavsky

In:

Nat Commun. (2020) 11: 215

Long-Term Expansion of Functional Human Pluripotent Stem Cell-Derived Hepatic Organoids

Categories:

Primary Cells and Media, 3D Cell Culture , Serum-free and Speciality Media

Authors:

Seon Ju Mun, Yeon-Hwa Hong , Hyo-Suk Ahn , Jae-Sung Ryu , Kyung-Sook Chung , Myung Jin Son

In:

(2020) 13: 2

Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease

Categories:

Primary Cells and Media, 3D Cell Culture

Authors:

Bin Ramli MN, Lim YS, Koe CT, Demircioglu D, Tng W, Uy Gonzales KA, Tan CP, Szczerbinska I, Liang H, Soe EL, Lu Z, Ariyachet C, Yu KM, Koh SH, Yaw LP, Binte Jumat NH, Yew Lim JS, Wright G, Shabbir A, Dan YY, Ng H-H, Chan Y-S

In:

World J Gastroenterol (2020) 159(4): 1471-1486

Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells

Categories:

Primary Cells and Media, 3D Cell Culture , Serum-free and Speciality Media, Transfection

Authors:

Kazuki Takeishi, Alexandra Collin de l’Hortet, Yang Wang, Kan Handa, Jorge Guzman-Lepe, Kentaro Matsubara, Kazutoyo Morita, Sae Jang, Nils Haep, Rodrigo M. Florentino, Fangchao Yuan, Ken Fukumitsu, Kimimasa Tobita, Wendell Sun, Jonatha Franks, Evan R. Delgado, Erik M. Shapiro, Nicolas A. Fraunhoffer, Andrew W. Duncan, Hiroshi Yagi, Tomoji Mashimo, Ira J. Fox, and Alejandro Soto-Gutierrez

In:

Cell Rep (2020) 31(9): 107711

Comparison of commercially available media for hepatic differentiation and hepatocyte maintenance

Categories:

Primary Cells and Media

Authors:

Toba Y, Deguchi S, Mimura N, Sakamoto A, Harada K, Hirata K, Takayama K, Mizuguchi H.

In:

PLoS ONE (2020) 15: e0229654

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

Categories:

Transfection

Authors:

Wienert B, Nguyen DN, Guenther A, Feng SJ, Locke MN, Wyman SK, Shin J, Kazane KR, Gregory GL, Carter MAM, Wright F, Conklin BR, Marson A, Richardson CD, Corn JE

In:

Nat Commun. (2020) 11(1): 2109

Generation of CCR5-ablated Human Induced Pluripotent Stem Cells as a Therapeutic Approach for Immune-mediated Diseases

Categories:

Transfection

Authors:

Negin Hosseini Rouzbahani , Saeid Kaviani , Mohammad Vasei , Masoud Soleimani , Kayhan Azadmanesh , Mohammad Hossein Nicknam

In:

Allergy Asthma Immunol Res. (2019) 18(3): 310-319

Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders.

Categories:

Primary Cells and Media, Transfection

Authors:

Sauter EJ, Kutsche LK, Klapper SD, Busskamp V.

In:

Methods Mol Biol (2019) 1942: 101-121

Human blood vessel organoids as a model of diabetic vasculopathy

Categories:

Primary Cells and Media, Transfection

Authors:

Wimmer RA, Leopoldi A, Aichinger M, Wick N, Hantusch B, Novatchkova M, Taubenschmid J, Hämmerle M, Esk C, Bagley JA, Lindenhofer D, Chen G, Boehm M, Agu CA, Yang F, Fu B, Zuber J, Knoblich JA, Kerjaschki D, Penninger JM.

In:

Nature (2019) 565 (7740): 505-510

Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies

Categories:

Transfection

Authors:

Paredes BD, Martins GLS, Azevedo CM, Sampaio GLA, Nonaka CKV, Silva KND, Soares MBP, Santos RRD, Souza BSF

In:

Stem Cell Res (2019) 38: 101454

Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells

Categories:

Transfection

Authors:

Madsen RR, Semple RK

In:

(2019) 11;4: 37

Efficient CRISPR/Cas9-mediated editing of trinucleotide repeat expansion in myotonic dystrophy patient-derived iPS and myogenic cells.

Categories:

Primary Cells and Media, Transfection

Authors:

Dastidar S, Ardui S, Singh K, Majumdar D, Nair N, Fu Y, Reyon D, Samara E, Gerli MFM, Klein AF, De Schrijver W, Tipanee J, Seneca S, Tulalamba W, Wang H, Chai YC, In't Veld P, Furling D, Tedesco FS, Vermeesch JR, Joung JK, Chuah MK, VandenDriessche T.

In:

Nucleic Acids Res (2018) 46(16): 8275-8298

Generation of integration-free iPS cell lines from three sickle cell disease patients from the state of Bahia, Brazil

Categories:

Transfection

Authors:

Martins GLS, Paredes BD, Azevedo CM, Sampaio GLA, Nonaka CKV, Cavalcante BRR, Da Silva KN, Pereira CSE, Soares MBP, Dos Santos RR, Souza BSF

In:

Stem Cell Res (2018) 33: 10-14

FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson’s Disease Modeling

Categories:

Transfection

Authors:

Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, Nickels SL, Zaehres H, Schöler HR, Schwamborn JC

In:

Stem Cell Reports (2017) 14;9(5): 1423-1431

CRISPR-Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice.

Categories:

Transfection

Authors:

Zhang Y, Long C, Li H, McAnally JR, Baskin KK1,2,3, Shelton JM4, Bassel-Duby R, Olson EN,

In:

Sci Adv. (2017) 12, 3(4): 1-11

A Genome-wide Analysis of Human Pluripotent Stem Cell-Derived Endothelial Cells in 2D or 3D Culture.

Categories:

3D Cell Culture

Authors:

Zhang J, Schwartz MP, Hou Z, Bai Y, Ardalani H, Swanson S, Steill J, Ruotti V, Elwell A, Nguyen BK, Bolin J, Stewart R, Thomson JA, Murphy WL

In:

Stem Cell Reports (2017) 8(4): 907-918

A Drug Screen using Human iPSC-Derived Hepatocyte-like Cells Reveals Cardiac Glycosides as a Potential Treatment for Hypercholesterolemia

Categories:

Primary Cells and Media, Serum-free and Speciality Media

Authors:

Max A Cayo , Sunil K Mallanna, Francesca Di Furio , Ran Jing , Lauren B Tolliver , Matthew Bures , Amanda Urick , Fallon K Noto, Evanthia E Pashos , Matthew D Greseth , Maciej Czarnecki , Paula Traktman , Wenli Yang , Edward E Morrisey, Markus Grompe , Daniel J Rader , Stephen A Duncan

In:

Cell Stem Cell (2017) 20: 4

Generation of human pluripotent stem cell-derived hepatocyte-like cells for drug toxicity screening.

Categories:

3D Cell Culture

Authors:

Takayama K, Mizuguchi H

In:

Drug Metab Pharmacokinet (2017) 32(1): 12-20

Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies.

Categories:

Transfection

Authors:

Wang G, Yang L, Grishin D, Rios X, Ye LY, Hu Y, Li K, Zhang D, Church GM, Pu WT.

In:

Nat Protocols (2017) 12(1): 88-103

Rhesus iPSC Safe Harbor Gene-Editing Platform for Stable Expression of Transgenes in Differentiated Cells of All Germ Layers.

Categories:

Transfection

Authors:

Hong SG, Yada RC, Choi K, Carpentier A, Liang TJ, Merling RK, Sweeney CL, Malech HL, Jung M, Corat MAF, AlJanahi AA, Lin Y, Liu H, Tunc I, Wang X, Palisoc M, Pittaluga S, Boehm M, Winkler T, Zou J, Dunbar CE.

In:

Mol Ther (2017) 25(1): 44-53

Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Chal J, Al Tanoury Z, Hestin M, Gobert B, Aivio S, Hick A, Cherrier T, Nesmith AP, Parker KK, Pourquié O

In:

Nat Protocols (2016) 11(10): 1833-50

Combining single-strand oligodeoxynucleotides and CRISPR/Cas9 to correct gene mutations in ß-thalassemia-induced Pluripotent Stem Cells

Categories:

Transfection

Authors:

Xiaohua Niu, Wenyin He, Bing Song, Zhanhui Ou, Di Fan, Yuchang Chen, Yong Fan, Xiaofang Sun

In:

J Biol Chem (2016) 291(32): 16576-85

Generation of a human induced pluripotent stem cell line via CRISPR-Cas9 mediated integration of a site-specific homozygous mutation in CHMP2B.

Categories:

Transfection

Authors:

Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, Freude KK.

In:

Stem Cell Res (2016) 17(1): 151-153

Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells.

Categories:

Primary Cells and Media

Authors:

Peters DT, Henderson CA, Warren CR, Friesen M, Xia F, Becker CE, Musunuru K, Cowan CA

In:

Development (2016) 143(9): 1475-1481

A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells.

Categories:

Transfection

Authors:

Young CS, Hicks MR, Ermolova NV, Nakano H, Jan M, Younesi S, Karumbayaram S, Kumagai-Cresse C, Wang D, Zack JA, Kohn DB, Nakano A, Nelson SF, Miceli MC, Spencer MJ, Pyle AD.

In:

Cell Stem Cell (2016) 18(4): 533-40

CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs

Categories:

Transfection

Authors:

Mohammad A. Mandegar, Nathaniel Huebsch, Ekaterina B. Frolov, Edward Shin, Annie Truong, Michael P. Olvera, Amanda H. Chan, Yuichiro Miyaoka, Kristin Holmes, C. Ian Spencer, Luke M. Judge, David E. Gordon, Tilde V. Eskildsen, Jacqueline E. Villalta, Max A. Horlbeck, Luke A. Gilbert, Nevan J. Krogan, Søren P. Sheikh, Jonathan S. Weissman, Lei S. Qi, Po-Lin So, and Bruce R. Conklin

In:

Cell Stem Cell (2016) 18 (4): 541-53

Genome Editing of the CYP1A1 Locus in iPSCs as a Platform to Map AHR Expression throughout Human Development

Categories:

Transfection

Authors:

BrendenW. Smith, Elizabeth A. Stanford, David H. Sherr, and George J.Murphy

In:

Stem Cells Intl (2016) 2016: 1-11

Optogenetic stimulation of multiwell MEA plates for neural and cardiac applications

Categories:

Primary Cells and Media

Authors:

Clements IP, Millard DC, Nicolini AM, Preyer AJ, Grier R, Heckerling A, Blum RA, Tyler P, McSweeney KM, Lu Y,Hall D, Ross JD

In:

Other (2016) 9690: ePub

Generation of an isogenic, gene-corrected control cell line of the spinocerebellar ataxia type 2 patient-derived iPSC line H196

Categories:

Transfection

Authors:

Adele G. Marthaler, Benjamin Schmid, Alisa Tubsuwan, Ulla B. Poulsenc, Alexander F. Engelbrecht,Ulrike A. Mau-Holzmann , Poul Hyttel , Jørgen E. Nielsen, Troels T. Nielsenb, Bjørn Holst

In:

Stem Cell Res (2016) 16: 162-165

Generation of Naivetropic Induced Pluripotent Stem Cells from Parkinson's Disease Patients for High-Efficiency Genetic Manipulation and Disease Modeling.

Categories:

Transfection

Authors:

Hu Z, Pu J, Jiang H, Zhong P, Qiu J, Li F, Wang X, Zhang B, Yan Z, Feng J1.

In:

Stem Cells Dev (2015) 24(21): 2591-25604

CRISPR/Cas9-mediated heterozygous knockout of the autism gene CHD8 and characterization of its transcriptional networks in neurodevelopment

Categories:

Transfection

Authors:

Ping Wang, Mingyan Lin, Erika Pedrosa, Anastasia Hrabovsky, Zheng Zhang, Wenjun Guo, Herbert M. Lachman,and Deyou Zheng

In:

Molecular Autism (2015) 6(55): 0.1186/s13229-015-0048-6

Generation of Isogenic Human iPS Cell Line Precisely Corrected by Genome Editing Using the CRISPR/Cas9 System.

Categories:

Transfection

Authors:

Grobarczyk B, Franco B, Hanon K, Malgrange B.

In:

Stem Cells (2015) 11(5): 774-87

Genomic DISC1 Disruption in hiPSCs Alters Wnt Signaling and Neural Cell Fate

Categories:

Transfection

Authors:

Priya Srikanth, Karam Han, Dana G. Callahan, Eugenia Makovkina, Christina R. Muratore, Matthew A. Lalli, Honglin Zhou, Justin D. Boyd, Kenneth S. Kosik, Dennis J. Selkoe, Tracy L. Young-Pearse

In:

Cell Rep (2015) 12: 1-16

A Comparative View on Easy to Deploy non-Integrating Methods for Patient-Specific iPSC Production.

Categories:

Transfection

Authors:

Manzini S, Viiri LE, Marttila S, Aalto-Setälä K

In:

Stem Cell Reports (2015) epub: 1-9

Functional Gene Correction for Cystic Fibrosis in Lung Epithelial Cells Generated from Patient iPSCs.

Categories:

Transfection

Authors:

Firth AL, Menon T, Parker GS, Qualls SJ, Lewis BM, Ke E, Dargitz CT, Wright R1, Khanna A, Gage FH, Verma IM

In:

Cell Rep (2015) 12(9): 1385-1390

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

Categories:

Transfection

Authors:

Raj Chari, Prashant Mali, Mark Moosburner, George M Church

In:

Nat Methods (2015) 12(9): 823-6

Pathogenesis of ELANE-mutant severe neutropenia revealed by induced pluripotent stem cells

Categories:

Transfection

Authors:

Nayak RC, Trump LR, Aronow BJ, Myers K, Mehta P, Kalfa T, Wellendorf AM, Valencia CA, Paddison PJ, Horwitz MS, Grimes HL, Lutzko C, Cancelas JA

In:

J Clin Invest (2015) 125(8): 3103-16

Prediction of drug-induced nephrotoxicity and injury mechanisms with human induced pluripotent stem cell-derived cells and machine learning methods.

Categories:

Primary Cells and Media

Authors:

Kandasamy K1, Chuah JK1, Su R2, Huang P1, Eng KG1, Xiong S1, Li Y1, Chia CS1, Loo LH3, Zink D1

In:

Scientific Reports (2015) 27(5): 12337

Rescue of DNA-PK Signaling and T-Cell Differentiation by Targeted Genome Editing in a prkdc Deficient iPSC Disease Model.

Categories:

Transfection

Authors:

Rahman SH, Kuehle J, Reimann C, Mlambo T, Alzubi J, Maeder ML, Riedel H, Fisch P, Cantz T, Rudolph C, Mussolino C, Joung JK, Schambach A, Cathomen T

In:

PLoS Genet (2015) 11(5): e1005239

Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature.

Categories:

Transfection

Authors:

Thomas SM, Kagan C, Pavlovic BJ, Burnett J, Patterson K, Pritchard JK, Gilad Y

In:

PLoS Genet (2015) 11(5): e1005216

Highly efficient Cas9-mediated transcriptional programming.

Categories:

Transfection

Authors:

Chavez A1, Scheiman J2, Vora S3, Pruitt BW4, Tuttle M4, P R Iyer E2, Lin S5, Kiani S6, Guzman CD4, Wiegand DJ4, Ter-Ovanesyan D2, Braff JL4, Davidsohn N2, Housden BE7, Perrimon N7, Weiss R8, Aach J9, Collins JJ10, Church GM2.

In:

Nat Methods (2015) 12(4): 326-8

Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach

Categories:

Transfection

Authors:

Debowski K, Warthemann R, Lentes J, Salinas-Riester G, Dressel R, Langenstroth D, Gromoll J, Sasaki E, Behr R.

In:

PLoS ONE (2015) 10(3): 1-21

A platform for rapid generation of single and multiplexed reporters in human iPSC lines

Categories:

Transfection

Authors:

Pei Y, Sierra G, Sivapatham R, Swistowski A, Rao MS, Zeng X

In:

Scientific Reports (2015) 5: 9205

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

Categories:

Transfection

Authors:

Susan M. Byrne, Luis Ortiz, Prashant Mali, John Aach, George M. Church

In:

Nucleic Acids Res (2015) 43(3): e21

Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines.

Categories:

Transfection

Authors:

Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J.

In:

PLoS ONE (2015) 10(1): 1-18

Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells

Categories:

Transfection

Authors:

Luhan Yang, Dennis Grishin, Gang Wang, John Aach, Cheng-Zhong Zhang, Raj Chari, Jason Homsy, Xuyu Cai, Yue Zhao, Jian-Bing Fan, Christine Seidman, Jonathan Seidman, William Pu, George Church

In:

Nat Commun. (2014) 5: 5507

Molecular beacon-enabled purification of living cells by targeting cell type-specific mRNAs

Categories:

Transfection

Authors:

Brian M Wile, Kiwon Ban, Young-Sup Yoon, Gang Bao

In:

Nat Protocols (2014) 9(10): 2411-24

Nuclease-mediated double-strand break (DSB) enhancement of small fragment homologous recombination (SFHR) gene modification in human-induced pluripotent stem cells (hiPSCs).

Categories:

Transfection

Authors:

Sargent RG, Suzuki S, Gruenert DC.

In:

Methods Mol Biol (2014) 1114: 279-90

Stable enhanced green fluorescent protein expression after differentiation and transplantation of reporter human induced pluripotent stem cells generated by AAVS1 transcription activator-like effector nucleases.

Categories:

Transfection

Authors:

Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao MS, Zou J.

In:

STEM CELLS TRANSLATIONALMEDICINE (2014) 3(7): 821-35

DICE, an efficient system for iterative genomic editing in human pluripotent stem cells.

Categories:

Transfection

Authors:

Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schüle B, Chen-Tsai Y, Calos MP.

In:

Proc Natl Acad Sci USA (2014) 42(5): e34

Nanog Overexpression Increases Neural Marker Expression in Adipose Derived Stem Cells

Categories:

Primary Cells and Media

Authors:

Kimmel J, Sugaya K

In:

Other (2014) 4(1): 2210-2973

Genome Editing in Human Stem Cells

Categories:

Transfection

Authors:

Susan M. Byrne, Prashant Mali, and George M. Church

In:

Methods Enzymol (2014) 546: 119-37

Generation of Human Induced Pluripotent Stem Cells Using Epigenetic Regulators Reveals a Germ Cell-Like Identity in Partially Reprogrammed Colonies

Categories:

Transfection

Authors:

Goyal A, Chavez SL, Reijo Pera RA.

In:

PLoS ONE (2013) 8(12): e82838

A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific induced pluripotent stem (iPS) cells

Categories:

Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Goh PA, Caxaria S, Casper C, Rosales C, Warner TT, Coffey PJ, Nathwani AC

In:

PLoS ONE (2013) 8(11): e81622

Optimization of scarless human stem cell genome editing.

Categories:

Transfection

Authors:

Yang L, Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang PY, Daley G, Church G.

In:

Nucleic Acids Res (2013) 41(19): 9049-61

RNA-guided human genome engineering via Cas9

Categories:

Transfection

Authors:

Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM

In:

Science (2013) 339(6121): 823-826

From skin biopsy to neurons through a pluripotent intermediate under Good Manufacturing Practice protocols

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Karumbayaram S, Lee P, Azghadi SF, Cooper AR, Patterson M, Kohn DB, Pyle A, Clark A, Byrne J, Zack JA, Plath K, Lowry WE

In:

STEM CELLS TRANSLATIONALMEDICINE (2012) 1(1): 36-43

Growth of engineered human myocardium with mechanical loading and vascular coculture.

Categories:

Primary Cells and Media

Authors:

Tulloch NL, Muskheli V, Razumova MV, Korte FS, Regnier M, Hauch KD, Pabon L, Reinecke H, Murry CE.

In:

Circ Res (2011) 109(1): 47-59

Highly efficient generation of human hepatocyte–like cells from induced pluripotent stem cells

Categories:

Authors:

Karim Si-Tayeb , Fallon K Noto, Masato Nagaoka, Jixuan Li, Michele A Battle, Christine Duris, Paula E North, Stephen Dalton, Stephen A Duncan

In:

Hepatology (2010) 1: 297

Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders.

Categories:

Primary Cells and Media

Authors:

Ye Z, Zhan H, Mali P, Dowey S, Williams DM, Jang YY, Dang CV, Spivak JL, Moliterno AR, Cheng L

In:

Blood (2009) 114(27): 5473-80

Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells.

Categories:

Transfection

Authors:

Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L.

In:

Stem Cells (2009) 5(1): 97-110

Human induced pluripotent stem cells free of vector and transgene sequences.

Categories:

Transfection

Authors:

Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA.

In:

Science (2009) 324(5928): 797-801

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

Categories:

Primary Cells and Media, 3D Cell Culture , Serum-free and Speciality Media

Authors:

Keisuke Sekine , Shimpei Ogawa, Syusaku Tsuzuki, Tatsuya Kobayashi , Kazuki Ikeda, Noriko Nakanishi , Kenta Takeuchi , Eriko Kanai , Yugo Otake , Satoshi Okamoto , Tsuyoshi Kobayashi , Takanori Takebe , Hideki Taniguchi

In:

Scientific Reports () 10: 17937

Standard operating protocol of hepatic organoid differentiation from human induced pluripotent stem cells

Categories:

Primary Cells and Media, 3D Cell Culture

Authors:

Min Jung Kim, Jaeseo Lee , Seon Ju Mun, Myung Jin Son, Jung-Hyun Kim

In:

Other () 2: e5

High temporal resolution proteome and phosphoproteome profiling of stem cell-derived hepatocyte development

Categories:

Primary Cells and Media, Serum-free and Speciality Media

Authors:

Johannes Krumm, Keisuke Sekine , Patroklos Samaras, Agnieska Brazovskaja , Markus Breunig , Ryota Yasui , Alexander Kleger , Hideki Taniguchi , Mathias Wilhelm , Barbara Treutlein , J Gray Camp , Bernhard Kuster

In:

Cell Rep () 38: 110604

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Induced Pluripotent Stem Cell (iPS), human

Transfection Information

Citations (82)