Induced Pluripotent Stem Cell (iPS), human

Pluripotent stem cell artificially derived from a non-pluripotent cell, typically an adult somatic cell, by inducing a forced expression of certain genes.

Cell Type:
Misc. Somatic SC
Tissue Origin:
Species:
human
Research Area:
Dermatology/Tissue Engineering
Stem Cells
Basic Research
Regenerative medicine
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform


2e5 50-75% 50-75% Plasmid (general) 0.4 µg 20 µl Shuttle


2e5 50-75% 50-75% Plasmid (general) 0.4 µg 20 µl 4D X-Unit


8e5 50-60% 50-75% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (48)

Categories:
Primary Cells and Media, Transfection 
Authors:
Dastidar S, Ardui S, Singh K, Majumdar D, Nair N, Fu Y, Reyon D, Samara E, Gerli MFM, Klein AF, De Schrijver W, Tipanee J, Seneca S, Tulalamba W, Wang H, Chai YC, In't Veld P, Furling D, Tedesco FS, Vermeesch JR, Joung JK, Chuah MK, VandenDriessche T.  
In:
Nucleic Acids Res (2018) 46(16): 8275-8298 
Categories:
Transfection 
Authors:
Zhang Y, Long C, Li H, McAnally JR, Baskin KK1,2,3, Shelton JM4, Bassel-Duby R, Olson EN, 
In:
Sci Adv. (2017) 12, 3(4): 1-11 
Categories:
3D Cell Culture  
Authors:
Zhang J, Schwartz MP, Hou Z, Bai Y, Ardalani H, Swanson S, Steill J, Ruotti V, Elwell A, Nguyen BK, Bolin J, Stewart R, Thomson JA, Murphy WL 
In:
Stem Cell Reports (2017) 8(4): 907-918 
Categories:
3D Cell Culture  
Authors:
Takayama K, Mizuguchi H 
In:
Drug Metab Pharmacokinet (2017) 32(1): 12-20 
Categories:
Transfection 
Authors:
Wang G, Yang L, Grishin D, Rios X, Ye LY, Hu Y, Li K, Zhang D, Church GM, Pu WT. 
In:
Nat Protocols (2017) 12(1): 88-103 
Categories:
Transfection 
Authors:
Hong SG, Yada RC, Choi K, Carpentier A, Liang TJ, Merling RK, Sweeney CL, Malech HL, Jung M, Corat MAF, AlJanahi AA, Lin Y, Liu H, Tunc I, Wang X, Palisoc M, Pittaluga S, Boehm M, Winkler T, Zou J, Dunbar CE. 
In:
Mol Ther (2017) 25(1): 44-53 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chal J, Al Tanoury Z, Hestin M, Gobert B, Aivio S, Hick A, Cherrier T, Nesmith AP, Parker KK, Pourquié O 
In:
Nat Protocols (2016) 11(10): 1833-50 
Categories:
Transfection 
Authors:
Xiaohua Niu, Wenyin He, Bing Song, Zhanhui Ou, Di Fan, Yuchang Chen, Yong Fan, Xiaofang Sun 
In:
J Biol Chem (2016) 291(32): 16576-85 
Categories:
Transfection 
Authors:
Zhang Y, Schmid B, Nielsen TT, Nielsen JE, Clausen C, Hyttel P, Holst B, Freude KK. 
In:
Stem Cells Res. (2016) 17(1): 151-153 
Categories:
 
Authors:
Peters DT, Henderson CA, Warren CR, Friesen M, Xia F, Becker CE, Musunuru K, Cowan CA 
In:
Development (2016) 143(9): 1475-1481 
Categories:
Transfection 
Authors:
Young CS, Hicks MR, Ermolova NV, Nakano H, Jan M, Younesi S, Karumbayaram S, Kumagai-Cresse C, Wang D, Zack JA, Kohn DB, Nakano A, Nelson SF, Miceli MC, Spencer MJ, Pyle AD. 
In:
Cell Stem Cell (2016) 18(4): 533-40 
Categories:
 
Authors:
Mohammad A. Mandegar, Nathaniel Huebsch, Ekaterina B. Frolov, Edward Shin, Annie Truong, Michael P. Olvera, Amanda H. Chan, Yuichiro Miyaoka, Kristin Holmes, C. Ian Spencer, Luke M. Judge, David E. Gordon, Tilde V. Eskildsen, Jacqueline E. Villalta, Max A. Horlbeck, Luke A. Gilbert, Nevan J. Krogan, Søren P. Sheikh, Jonathan S. Weissman, Lei S. Qi, Po-Lin So, and Bruce R. Conklin 
In:
Cell Stem Cell (2016) 18 (4): 541-53 
Categories:
Transfection 
Authors:
BrendenW. Smith, Elizabeth A. Stanford, David H. Sherr, and George J.Murphy 
In:
Stem Cells Intl (2016) 2016: 1-11 
Categories:
Primary Cells and Media 
Authors:
Clements IP, Millard DC, Nicolini AM, Preyer AJ, Grier R, Heckerling A, Blum RA, Tyler P, McSweeney KM, Lu Y,Hall D, Ross JD 
In:
Other (2016) 9690: ePub 
Categories:
Primary Cells and Media 
Authors:
Clements IP, Millard DC, Nicolini AM, Preyer AJ, Grier R, Heckerling A, Blum RA, Tyler P, McSweeney KM, Lu Y,Hall D, Ross JD 
In:
Other (2016) 9690: ePub 
Categories:
Transfection 
Authors:
Adele G. Marthaler, Benjamin Schmid, Alisa Tubsuwan, Ulla B. Poulsenc, Alexander F. Engelbrecht,Ulrike A. Mau-Holzmann , Poul Hyttel , Jørgen E. Nielsen, Troels T. Nielsenb, Bjørn Holst 
In:
Stem Cells Res. (2016) 16: 162-165 
Categories:
Transfection 
Authors:
Hu Z,, Pu J, Jiang H, Zhong P, Qiu J, Li F, Wang X, Zhang B, Yan Z, Feng J1. 
In:
Stem Cells Dev (2015) 24(21): 2591-25604 
Categories:
Transfection 
Authors:
Ping Wang, Mingyan Lin, Erika Pedrosa, Anastasia Hrabovsky, Zheng Zhang, Wenjun Guo, Herbert M. Lachman,and Deyou Zheng 
In:
Molecular Autism (2015) 6(55): 0.1186/s13229-015-0048-6 
Categories:
Transfection 
Authors:
Grobarczyk B, Franco B, Hanon K, Malgrange B. 
In:
Stem Cells (2015) 11(5): 774-87 
Categories:
Transfection 
Authors:
Priya Srikanth, Karam Han, Dana G. Callahan, Eugenia Makovkina, Christina R. Muratore, Matthew A. Lalli, Honglin Zhou, Justin D. Boyd, Kenneth S. Kosik, Dennis J. Selkoe, Tracy L. Young-Pearse 
In:
Cell Rep (2015) 12: 1-16 
Categories:
Transfection 
Authors:
Manzini S, Viiri LE, Marttila S, Aalto-Setälä K 
In:
Stem Cell Reports (2015) epub: 1-9 
Categories:
Transfection 
Authors:
Firth AL, Menon T, Parker GS, Qualls SJ, Lewis BM, Ke E, Dargitz CT, Wright R1, Khanna A, Gage FH, Verma IM 
In:
Cell Rep (2015) 12(9): 1385-1390 
Categories:
Transfection 
Authors:
Raj Chari, Prashant Mali, Mark Moosburner, George M Church 
In:
Nat Methods (2015) 12(9): 823-6 
Categories:
Transfection 
Authors:
Nayak RC, Trump LR, Aronow BJ, Myers K, Mehta P, Kalfa T, Wellendorf AM, Valencia CA, Paddison PJ, Horwitz MS, Grimes HL, Lutzko C, Cancelas JA 
In:
J Clin Invest (2015) 125(8): 3103-16 
Categories:
 
Authors:
Kandasamy K1, Chuah JK1, Su R2, Huang P1, Eng KG1, Xiong S1, Li Y1, Chia CS1, Loo LH3, Zink D1 
In:
Scientific Reports (2015) 27(5): 12337 
Categories:
Transfection 
Authors:
Rahman SH, Kuehle J, Reimann C, Mlambo T, Alzubi J, Maeder ML, Riedel H, Fisch P, Cantz T, Rudolph C, Mussolino C, Joung JK, Schambach A, Cathomen T 
In:
PLoS Genet (2015) 11(5): e1005239 
Categories:
Transfection 
Authors:
Thomas SM, Kagan C, Pavlovic BJ, Burnett J, Patterson K, Pritchard JK, Gilad Y 
In:
PLoS Genet (2015) 11(5): e1005216 
Categories:
Transfection 
Authors:
Chavez A1, Scheiman J2, Vora S3, Pruitt BW4, Tuttle M4, P R Iyer E2, Lin S5, Kiani S6, Guzman CD4, Wiegand DJ4, Ter-Ovanesyan D2, Braff JL4, Davidsohn N2, Housden BE7, Perrimon N7, Weiss R8, Aach J9, Collins JJ10, Church GM2. 
In:
Nat Methods (2015) 12(4): 326-8 
Categories:
Transfection 
Authors:
Debowski K, Warthemann R, Lentes J, Salinas-Riester G, Dressel R, Langenstroth D, Gromoll J, Sasaki E, Behr R. 
In:
PLoS ONE (2015) 10(3): 1-21 
Categories:
Transfection 
Authors:
Pei Y, Sierra G, Sivapatham R, Swistowski A, Rao MS, Zeng X 
In:
Scientific Reports (2015) 5: 9205 
Categories:
Transfection 
Authors:
Susan M. Byrne, Luis Ortiz, Prashant Mali, John Aach, George M. Church 
In:
Nucleic Acids Res (2015) 43(3): e21 
Categories:
Transfection 
Authors:
Luhan Yang, Dennis Grishin, Gang Wang, John Aach, Cheng-Zhong Zhang, Raj Chari, Jason Homsy, Xuyu Cai, Yue Zhao, Jian-Bing Fan, Christine Seidman, Jonathan Seidman, William Pu, George Church 
In:
Nat Commun. (2014) 5: 5507 
Categories:
Transfection 
Authors:
Brian M Wile, Kiwon Ban, Young-Sup Yoon, Gang Bao 
In:
Nat Protocols (2014) 9(10): 2411-24 
Categories:
Transfection 
Authors:
Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao MS, Zou J. 
In:
STEM CELLS TRANSLATIONALMEDICINE (2014) 3(7): 821-35 
Categories:
Transfection 
Authors:
Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schüle B, Chen-Tsai Y, Calos MP. 
In:
Proc Natl Acad Sci USA (2014) 42(5): e34 
Categories:
Primary Cells and Media 
Authors:
Kimmel J, Sugaya K 
In:
Other (2014) 4(1): 2210-2973 
Categories:
Transfection 
Authors:
Susan M. Byrne, Prashant Mali, and George M. Church 
In:
Methods Enzymol (2014) 546: 119-37 
Categories:
Transfection 
Authors:
Goyal A, Chavez SL, Reijo Pera RA. 
In:
PLoS ONE (2013) 8(12): e82838 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Goh PA, Caxaria S, Casper C, Rosales C, Warner TT, Coffey PJ, Nathwani AC 
In:
PLoS ONE (2013) 8(11): e81622 
Categories:
Transfection 
Authors:
Yang L, Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang PY, Daley G, Church G. 
In:
Nucleic Acids Res (2013) 41(19): 9049-61 
Categories:
Transfection 
Authors:
Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM 
In:
Science (2013) 339(6121): 823-826 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Karumbayaram S, Lee P, Azghadi SF, Cooper AR, Patterson M, Kohn DB, Pyle A, Clark A, Byrne J, Zack JA, Plath K, Lowry WE 
In:
STEM CELLS TRANSLATIONALMEDICINE (2012) 1(1): 36-43 
Categories:
 
Authors:
Tulloch NL, Muskheli V, Razumova MV, Korte FS, Regnier M, Hauch KD, Pabon L, Reinecke H, Murry CE. 
In:
Circ Res (2011) 109(1): 47-59 
Categories:
Primary Cells and Media 
Authors:
Ye Z, Zhan H, Mali P, Dowey S, Williams DM, Jang YY, Dang CV, Spivak JL, Moliterno AR, Cheng L 
In:
Blood (2009) 114(27): 5473-80 
Categories:
Transfection 
Authors:
Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L. 
In:
Stem Cells (2009) 5(1): 97-110 
Categories:
Transfection 
Authors:
Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. 
In:
Science (2009) 324(5928): 797-801 
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