Lymphocyte Growth Medium-3

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth
Shelf Life:
12 months
Recommended for Cells:
THP-1 ; B cell, human ; T cell, human peripheral blood unstim. ; T cell, human stim. ; Dendritic cell (NHDC), human ; Natural killer Cells (NK), human ; PBMC, human ; Bone Marrow, Human, Unprocessed ; Mononuclear, bone marrow, human ; Mononuclear, cord blood, human ; Mononuclear, peripheral blood, human ; Mononuclear, bone marrow, dog ; Mononuclear, bone marrow, baboon ; Mononuclear, bone marrow, mouse
Categories:
Primary Cells and Media
Research Areas:
Immunotherapy / Hematology
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Citations (24)

Bacillus anthracis spores germinate extracellularly at air-liquid interface in an in vitro lung model under serum-free conditions.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Powell JD, Hutchison JR, Hess BM, Straub TM 
In:
Other (2015) 119(3): 711-23 
A vaccine strategy with multiple prostatic acid phosphatase-fused cytokines for prostate cancer treatment
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fujio K, Watanabe M, Ueki H, Li SA1, Kinoshita R, Ochiai K, Futami J, Watanabe T, Nasu Y, Kumon H. 
In:
Oncol Rep (2015) 4: 1585-92 
Uptake and Presentation of Myelin Basic Protein by Normal Human B Cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Marie Klinge Brimnes, Bjarke Endel Hansen, Leif Kofoed Nielsen, Morten Hanefeld Dziegiel, Claus Henrik Nielsen 
In:
PLoS ONE (2014) 17(9): e113388 
Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Maeß MB, Wittig B, Lorkowski S 
In:
J Vis Exp (2014) 91: 1-9 
Peptide Immunization Against ERG and Immunogenic Mutations to Treat Prostate Cancer
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Haydn T Kissick 
In:
Other (2014) : 1-56 
Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Larman HB, Scott ER, Wogan M, Oliveira G, Torkamani A, Schultz PG 
In:
Nucleic Acids Res (2014) 42(4): 9146-57 
HBD3 regulates matrix metalloproteinase production in human myeloid dendritic cells exposed to Porphyromonas gingivalis hemagglutinin B
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
Authors:
Raina M 
In:
Other (2014) : Thesis 
c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Micheva-Viteva SN, Shou Y, Nowak-Lovato KL, Rector KD, Hong-Geller E 
In:
BMC Microbiol. (2013) 13: 249 
Enhanced rho-associated protein kinase activation in patients with systemic lupus erythematosus.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Isgro J, Gupta S, Jacek E, Pavri T, Duculan R, Kim M, Kirou KA, Salmon JE, Pernis AB 
In:
Arthritis Rheum (2013) 65(6): 1592-602 
Sphingoid bases induce dose-dependent cytotoxicity and cytokine responses in human myeloid dendritic cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mehalick LA 
In:
Other (2013) 0:0: Thesis 
Defensin DEFB103 bidirectionally regulates chemokine and cytokine responses to a pro-inflammatory stimulus.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
Authors:
Harvey LE, Kohlgraf KG, Mehalick LA, Raina M, Recker EN, Radhakrishnan S, Prasad SA, Vidva R, Progulske-Fox A, Cavanaugh JE, Vali S, Brogden KA 
In:
Other (2013) 3: 1232 
EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Maddigan A, Truitt L, Arsenault R, Freywald T, Allonby O, Dean J, Narendran A, Xiang J, Weng A, Napper S, Freywald A 
In:
J Immunol (2011) 187(11): 5983-94 
A Role for Rab7 in the Movement of Secretory Granules in Cytotoxic T Lymphocytes
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Daniele T, Hackmann Y, Ritter AT, Wenham M, Booth S, Bossi G, Schintler M, Auer-Grumbach M, Griffiths GM. 
In:
Traffic (2011) 7: 902-911 
Development of an Alternative Test for Skin Sensitization Using a Three-dimensional Human Skin Model Consisting of Dendritic Cells, Keratinocytes and Fibroblasts .
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uchino T, Takezawa T, Ikarashi Y, Nishimura T 
In:
AATEX (2011) 16(1): 1-8 
Histatin 5 attenuates IL-8 dendritic cell response to gingivalis Hemagglutinin B
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Borgwardt DS 
In:
Other (2011) 0:0: Thesis 
MicroRNA-21 and microRNA-148a contribute to DNA hypomethylation in lupus CD4+ T cells by directly and indirectly targeting DNA methyltransferase 1.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Pan W, Zhu S, Yuan M, Cui H, Wang L, Luo X, Li J, Zhou H, Tang Y, Shen N. 
In:
J Immunol (2010) 184(12): 6773-81 
Immunological aspects of REIC/Dkk-3 in monocyte differentiation and tumor regression
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Watanabe M, Kashiwakura Y, Huang P, Ochiai K, Futami J, Li SA, Takaoka M, Nasu Y, Sakaguchi M, Huh NH, Kumon H. 
In:
Int J Oncol. (2009) 3: 657-63 
The Role of Phospholipase D (PLD) and Grb2 in Chemotaxis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Knapek KJ 
In:
Other (2008) 0:0: Thesis 
Construction of a Three-Dimensional Human Skin Model Consisting of Keratinocytes, Dendritic Cells and Fibroblasts and Application of This Model for Alternative Animal Testing of Immune-Sensitizing Compounds
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uchino T, Ikarashi Y, Tokunaga H 
In:
Other (2007) 41(4): 246-253 
Tyrosine kinase Syk associates with toll-like receptor 4 and regulates signaling in human monocytic cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chaudhary A, Fresquez TM, Naranjo MJ. 
In:
Immunol Cell Biol (2007) 85(3): 249-56 
Immunosuppressive effects of polyunsaturated fatty acids on antigen presentation by human leukocyte antigen class I molecules.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Shaikh SR, Edidin M 
In:
J Lipid Res (2007) 48(1): 127-38 
Replication of chimeric yellow fever virus-dengue serotype 1-4 virus vaccine strains in dendritic and hepatic cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Brandler S, Brown N, Ermak TH, Mitchell F, Parsons M, Zhang Z, Lang J, Monath TP, Guirakhoo F. 
In:
Am. J. Trop. Med (2005) 72(1): 74-81 
Extracellular Nef protein targets CD4+ T cells for apoptosis by interacting with CXCR4 surface receptors.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC 
In:
J Virol (2004) 78(6): 3099-109 
Induction of megakaryocytic differentiation in primary human erythroblasts: a physiological basis for leukemic lineage plasticity
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Goldfarb AN, Wong D, Racke FK 
In:
Am J Pathol (2001) 158(4): 1191-8 
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