Dendritic cell (NHDC), human

Immature or mature dendritic cells; monocyte-derived. DCs are antigen presenting cells for T cells and are typically found in the skin and mucosal membranes.

Cell Type:
Dendritic cell (DC)
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and human or recombinant transferrin.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
DC, human U-002 36-62% 71% Plasmid (general) 5 µg 100 µl I/II/2b
P3 CB-150 4e5 57-81% 82-86% mRNA 0.5 µg 20 µl 4D X-Unit
P3 CB-150 4e5 42-66% 79-89% mRNA 0.5 µg 20 µl 4D X-Unit
P3 CB-150 4e5 33-57% 77-81% mRNA 0.5 µg 20 µl 4D X-Unit
P3 EH-104 4e5 82-84% 51-57% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 CB-150 2e5 28-36% 96-98% mRNA 0.5 µg 20 µl 4D X-Unit
P3 CB-150 2e5 17-23% 96-98% mRNA 0.5 µg 20 µl 4D X-Unit
P3 EH-104 2e5 87% 84% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 EH-104 2e5 93% 71-81% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (57)

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Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Freund EC, Lock JY, Oh J, Maculins T, Delamarre L, Bohlen CJ, Haley B, Murthy A. 
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J Exp Med (2020) 217(7): e20191692 
Categories:
Primary Cells and Media, 3D Cell Culture , Classical Media and Reagents, Serum-free and Speciality Media 
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Clin Exp Immunol (2017) 52(4): 355(80) 
Categories:
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
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Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
Transfection 
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J Vis Exp (2011) 51: 2736 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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In:
Other (2011) 0:0: Thesis 
Categories:
Transfection 
Authors:
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Mol Ther (2009) 17(9): 1616-25 
Categories:
Transfection 
Authors:
Ye L, Liu X, Rout SN, Li Z, Yan Y, Lu L, Kamala T, Nanda NK, Song W, Samal SK, Zhu X 
In:
J Immunol (2008) 181(4): 2572-85 
Categories:
Transfection 
Authors:
Wang FX, Huang J, Zhang H, Ma X, Zhang H 
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J Gen Virol (2008) 89(Pt 3): 722-30 
Categories:
Transfection 
Authors:
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In:
J Immunol (2008) 180(2): 980-7 
Categories:
Transfection 
Authors:
de Witte L, Bobardt M, Chatterji U, Degeest G, David G, Geijtenbeek TB, Gallay P 
In:
Proc Natl Acad Sci USA (2007) 104(49): 19464-9 
Categories:
Transfection 
Authors:
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In:
J Leukoc Biol (2007) 82(4): 849-60 
Categories:
Transfection 
Authors:
Dong C, Janas AM, Wang JH, Olson WJ, Wu L 
In:
J Virol (2007) 81(20): 11352-62 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uchino T, Ikarashi Y, Tokunaga H 
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Other (2007) 41(4): 246-253 
Categories:
Transfection 
Authors:
Melhem NM, Liu XD, Boczkowski D, Gilboa E, Barratt-Boyes SM 
In:
Eur J Immunol (2007) 37(8): 2164-73 
Categories:
Transfection 
Authors:
Hamdi H, Godot V, Maillot MC, Prejean MV, Cohen N, Krzysiek R, Lemoine FM, Zou W, Emilie D 
In:
Blood (2007) 110(1): 211-9 
Categories:
Transfection 
Authors:
Geyeregger R, Zeyda M, Bauer W, Kriehuber E, Saemann MD, Zlabinger GJ, Maurer D, Stulnig TM 
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Blood (2007) 109(10): 4288-95 
Categories:
Transfection 
Authors:
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J Immunol (2007) 178(10): 6208-16 
Categories:
Transfection 
Authors:
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Proc Natl Acad Sci USA (2007) 104(21): 8947-52 
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Transfection 
Authors:
Pompei L, Jang S, Zamlynny B, Ravikumar S, McBride A, Hickman SP, Salgame P 
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J Immunol (2007) 178(8): 5192-9 
Categories:
Transfection 
Authors:
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J Leukoc Biol (2007) 81(1): 355-63 
Categories:
Transfection 
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Ito D, Albers A, Zhao YX, Visus C, Appella E, Whiteside TL, Deleo AB 
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J Immunol (2006) 177(10): 6795-803 
Categories:
Transfection 
Authors:
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J Virol (2006) 80(20): 10010-20 
Categories:
Transfection 
Authors:
van Helden SF, Krooshoop DJ, Broers KC, Raymakers RA, Figdor CG, van Leeuwen FN 
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J Immunol (2006) 177(3): 1567-74 
Categories:
Transfection 
Authors:
Stallwood Y, Briend E, Ray KM, Ward GA, Smith BJ, Nye E, Champion BR, McKenzie GJ 
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J Immunol (2006) 177(2): 885-95 
Categories:
Transfection 
Authors:
Chapatte L, Ayyoub M, Morel S, Peitrequin AL, Levy N, Servis C, Van den Eynde BJ, Valmori D, Levy F 
In:
Cancer Res (2006) 66(10): 5461-8 
Categories:
Transfection 
Authors:
Rethi B, Gogolak P, Szatmari I, Veres A, Erdos E, Nagy L, Rajnavolgyi E, Terhorst C, Lanyi A 
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Blood (2006) 107(7): 2821-9 
Categories:
Transfection 
Authors:
Sundrud MS, Vancompernolle SE, Eger KA, Bruno TC, Subramaniam A, Mummidi S, Ahuja SK and Unutmaz D 
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Blood (2005) 106(10): 3440-3448 
Categories:
Transfection 
Authors:
de Goer de Herve MG, Durali D, Tran TA, Maigne G, Simonetta F, Leclerc P, Delfraissy JF and Taoufik Y 
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Blood (2005) 106(8): 2806-2814 
Categories:
Transfection 
Authors:
Tourkova IL, Shurin GV, Chatta GS, Perez L, Finke J, Whiteside TL, Ferrone S and Shurin MR 
In:
J Immunol (2005) 175(5): 3045-3052 
Categories:
Transfection 
Authors:
Zeyda M, Saemann MD, Stuhlmeier KM, Mascher DG, Nowotny PN, Zlabinger GJ, Waldhausl W and Stulnig TM 
In:
J Biol Chem (2005) 280(14): 14293-14301 
Categories:
Transfection 
Authors:
Riol-Blanco L, Sanchez-Sanchez N, Torres A, Tejedor A, Narumiya S, Corbi AL, Sanchez-Mateos P and Rodriguez-Fernandez JL 
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J Immunol (2005) 174(7): 4070-4080 
Categories:
Transfection 
Authors:
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J Immunol (2005) 174(5): 2814-2824 
Categories:
Transfection 
Authors:
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Cancer Gene Ther (2005) 12(2): 185-197 
Categories:
Transfection 
Authors:
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J Immunol (2005) 174(3): 1751-1759 
Categories:
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Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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Categories:
Transfection 
Authors:
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Categories:
Transfection 
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Categories:
Transfection 
Authors:
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Categories:
Primary Cells and Media 
Authors:
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Categories:
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Authors:
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Categories:
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Categories:
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