Dendritic cell (NHDC), human

Immature or mature dendritic cells; monocyte-derived. DCs are antigen presenting cells for T cells and are typically found in the skin and mucosal membranes.

Cell Type:
Dendritic cell (DC)
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

TheraPEAK® X-VIVO® Media is a high-performing media series providing nutritionally adequate and balanced environments for a variety of hematopoietic cells including: 

  • T Cells
  • CAR-T cells
  • Peripheral blood lymphocytes (PBL)
  • Tumor infiltrating lymphocytes (TIL)
  • Human monocytes and macrophages
  • Lymphokine-activated killer cells (LAK)
  • Hematopoietic stem cells (HSCs)
  • Dendritic cells (DCs) 

Our scalable media are widely cited in scientific publications and proven to reliably work in many cell therapy applications around the world, from research stages to clinical trials to FDA-approved cell therapies. TheraPEAK® Products are manufactured according to GMP standards and can be safely and confidently used in your clinical processes. Our manufacturing sites are FDA registered with an ISO 13485 certified quality management system. 


There are two main versions of this media.

TheraPEAK® X-VIVO® 10 Media are  optimized for slower growing, less mature cells, such as hematopoietic stem cells

TheraPEAK® X-VIVO® 15 Media are most appropriate for rapidly growing cells, specifically cells of the immune system

TheraPEAK® X-VIVO® 10 Media and 15 Media are also available with recombinant transferrin instead of native human transferrin

Please note: X-VIVO™ 20 is currently available as an RUO grade catalog item. 

Storage: 2-8C, protect from light.

Catalog Offerings:

TheraPEAK® X-VIVO® Medium with native transferrin

Cat. No. NA

Cat.CCat. No. EU

Product

Size

BP04-743Q                            

 

           BEBP04-743Q

TheraPEAK® X-VIVO® 10 Medium without gentamicin and phenol red, contains native transferrin

1 L bottle

BP04-744Q

 

BEBP04-744Q

TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin

1 L bottle

08-879H

08-879P1

 

BE08-879H

BE08-879P1

TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin

5 L bag

1 L bag

TheraPEAK™ X-VIVO™ Medium with recombinant transferrin

Cat. No. NA

Cat. No. EU

Product

Size

N/A

BEBP02-055Q

TheraPEAK® X-VIVO® 10 Medium with L-glutamine, without gentamicin or phenol red, contains recombinant transferrin

1 L bottle

BP02-054Q

BP02-054P1

BEBP02-054Q

BEBP02-054P1

TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains recombinant transferrin

1 L bottle

1 L bag

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
DC, human U-002 36-62% 71% Plasmid (general) 5 µg 100 µl I/II/2b
P3 CB-150 4e5 57-81% 82-86% mRNA 0.5 µg 20 µl 4D X-Unit
P3 CB-150 4e5 42-66% 79-89% mRNA 0.5 µg 20 µl 4D X-Unit
P3 CB-150 4e5 33-57% 77-81% mRNA 0.5 µg 20 µl 4D X-Unit
P3 EH-104 4e5 82-84% 51-57% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 CB-150 2e5 28-36% 96-98% mRNA 0.5 µg 20 µl 4D X-Unit
P3 CB-150 2e5 17-23% 96-98% mRNA 0.5 µg 20 µl 4D X-Unit
P3 EH-104 2e5 87% 84% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 EH-104 2e5 93% 71-81% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (59)

Categories:
Transfection 
Authors:
Marco Jost, Amy N. Jacobson, Jeffrey A. Hussmann, Giana Cirolia,  Michael A. Fischbach,  Jonathan S. Weissman 
In:
BioResearch Open Access (2020) 1: 1-76 
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Freund EC, Lock JY, Oh J, Maculins T, Delamarre L, Bohlen CJ, Haley B, Murthy A. 
In:
J Exp Med (2020) 217(7): e20191692 
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Chandorkar P, Posch W, Zaderer V, Blatzer M, Steger M, Ammann CG, Binder U, Hermann M, Hörtnagl P, Lass-Flörl C, Wilflingseder D 
In:
Scientific Reports (2017) 7(1): 10.1038/s41598-017-11271-4 
Categories:
Transfection 
Authors:
Hudecek M1, Izsvák Z2, Johnen S3, Renner M4, Thumann G5, Ivics Z4. 
In:
Clin Exp Immunol (2017) 52(4): 355(80) 
Categories:
Serum-free and Speciality Media 
Authors:
Ellen J. Lee, Brieanna R. Brown, Emily E. Vance, Paige E. Snow, Phyllis B. Silver, David Heinrichs, Xin Lin, Yoichiro Iwakura, Christine A. Wells, Rachel R. Caspi and Holly L. Rosenzweig 
In:
J Immunol (2016) 196: 7 
Categories:
Primary Cells and Media 
Authors:
Liu G1, Bi Y2, Xue L3, Zhang Y4, Yang H4, Chen X4, Lu Y4, Zhang Z4, Liu H4, Wang X4, Wang R5, Chu Y4, Yang R2. 
In:
Proc Natl Acad Sci USA (2015) 112(9): E957-65 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
Authors:
Raina M 
In:
Other (2014) : Thesis 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Micheva-Viteva SN, Shou Y, Nowak-Lovato KL, Rector KD, Hong-Geller E 
In:
BMC Microbiol. (2013) 13: 249 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mehalick LA 
In:
Other (2013) 0:0: Thesis 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
Authors:
Harvey LE, Kohlgraf KG, Mehalick LA, Raina M, Recker EN, Radhakrishnan S, Prasad SA, Vidva R, Progulske-Fox A, Cavanaugh JE, Vali S, Brogden KA 
In:
Other (2013) 3: 1232 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uchino T, Takezawa T, Ikarashi Y, Nishimura T 
In:
AATEX (2011) 16(1): 1-8 
Categories:
Transfection 
Authors:
Gerdemann U, Vera JF, Rooney CM, Leen AM. 
In:
J Vis Exp (2011) 51: 2736 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Borgwardt DS 
In:
Other (2011) 0:0: Thesis 
Categories:
Transfection 
Authors:
Gerdemann U, Christin AS, Vera JF, Ramos CA, Fujita Y, Liu H, Dilloo D, Heslop HE, Brenner MK, Rooney CM, Leen AM. 
In:
Mol Ther (2009) 17(9): 1616-25 
Categories:
Transfection 
Authors:
Ye L, Liu X, Rout SN, Li Z, Yan Y, Lu L, Kamala T, Nanda NK, Song W, Samal SK, Zhu X 
In:
J Immunol (2008) 181(4): 2572-85 
Categories:
Transfection 
Authors:
Wang FX, Huang J, Zhang H, Ma X, Zhang H 
In:
J Gen Virol (2008) 89(Pt 3): 722-30 
Categories:
Transfection 
Authors:
Sloma I, Zilber MT, Vasselon T, Setterblad N, Cavallari M, Mori L, De Libero G, Charron D, Mooney N, Gelin C 
In:
J Immunol (2008) 180(2): 980-7 
Categories:
Transfection 
Authors:
de Witte L, Bobardt M, Chatterji U, Degeest G, David G, Geijtenbeek TB, Gallay P 
In:
Proc Natl Acad Sci USA (2007) 104(49): 19464-9 
Categories:
Transfection 
Authors:
Landi A, Babiuk LA, van Drunen Littel-van den Hurk S 
In:
J Leukoc Biol (2007) 82(4): 849-60 
Categories:
Transfection 
Authors:
Dong C, Janas AM, Wang JH, Olson WJ, Wu L 
In:
J Virol (2007) 81(20): 11352-62 
Categories:
Serum-free and Speciality Media 
Authors:
Sung Jung Kim, Betty Diamond 
In:
J Immunol Methods (2007) 323: 2007 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uchino T, Ikarashi Y, Tokunaga H 
In:
Other (2007) 41(4): 246-253 
Categories:
Transfection 
Authors:
Melhem NM, Liu XD, Boczkowski D, Gilboa E, Barratt-Boyes SM 
In:
Eur J Immunol (2007) 37(8): 2164-73 
Categories:
Transfection 
Authors:
Hamdi H, Godot V, Maillot MC, Prejean MV, Cohen N, Krzysiek R, Lemoine FM, Zou W, Emilie D 
In:
Blood (2007) 110(1): 211-9 
Categories:
Transfection 
Authors:
Geyeregger R, Zeyda M, Bauer W, Kriehuber E, Saemann MD, Zlabinger GJ, Maurer D, Stulnig TM 
In:
Blood (2007) 109(10): 4288-95 
Categories:
Transfection 
Authors:
Severa M, Remoli ME, Giacomini E, Annibali V, Gafa V, Lande R, Tomai M, Salvetti M, Coccia EM 
In:
J Immunol (2007) 178(10): 6208-16 
Categories:
Transfection 
Authors:
Valmori D, Souleimanian NE, Tosello V, Bhardwaj N, Adams S, O'neill D, Pavlick A, Escalon JB, Cruz CM, Angiulli A, Angiulli F, Mears G, Vogel SM, Pan L, Jungbluth AA, Hoffmann EW, Venhaus R, Ritter G, Old LJ, Ayyoub M 
In:
Proc Natl Acad Sci USA (2007) 104(21): 8947-52 
Categories:
Transfection 
Authors:
Pompei L, Jang S, Zamlynny B, Ravikumar S, McBride A, Hickman SP, Salgame P 
In:
J Immunol (2007) 178(8): 5192-9 
Categories:
Transfection 
Authors:
Remoli ME, Ragimbeau J, Giacomini E, Gafa V, Severa M, Lande R, Pellegrini S, Coccia EM 
In:
J Leukoc Biol (2007) 81(1): 355-63 
Categories:
Transfection 
Authors:
Ito D, Albers A, Zhao YX, Visus C, Appella E, Whiteside TL, Deleo AB 
In:
J Immunol (2006) 177(10): 6795-803 
Categories:
Transfection 
Authors:
Tang J, Murtadha M, Schnell M, Eisenlohr LC, Hooper J, Flomenberg P 
In:
J Virol (2006) 80(20): 10010-20 
Categories:
Transfection 
Authors:
van Helden SF, Krooshoop DJ, Broers KC, Raymakers RA, Figdor CG, van Leeuwen FN 
In:
J Immunol (2006) 177(3): 1567-74 
Categories:
Transfection 
Authors:
Stallwood Y, Briend E, Ray KM, Ward GA, Smith BJ, Nye E, Champion BR, McKenzie GJ 
In:
J Immunol (2006) 177(2): 885-95 
Categories:
Transfection 
Authors:
Chapatte L, Ayyoub M, Morel S, Peitrequin AL, Levy N, Servis C, Van den Eynde BJ, Valmori D, Levy F 
In:
Cancer Res (2006) 66(10): 5461-8 
Categories:
Transfection 
Authors:
Rethi B, Gogolak P, Szatmari I, Veres A, Erdos E, Nagy L, Rajnavolgyi E, Terhorst C, Lanyi A 
In:
Blood (2006) 107(7): 2821-9 
Categories:
Transfection 
Authors:
Sundrud MS, Vancompernolle SE, Eger KA, Bruno TC, Subramaniam A, Mummidi S, Ahuja SK and Unutmaz D 
In:
Blood (2005) 106(10): 3440-3448 
Categories:
Transfection 
Authors:
de Goer de Herve MG, Durali D, Tran TA, Maigne G, Simonetta F, Leclerc P, Delfraissy JF and Taoufik Y 
In:
Blood (2005) 106(8): 2806-2814 
Categories:
Transfection 
Authors:
Tourkova IL, Shurin GV, Chatta GS, Perez L, Finke J, Whiteside TL, Ferrone S and Shurin MR 
In:
J Immunol (2005) 175(5): 3045-3052 
Categories:
Transfection 
Authors:
Zeyda M, Saemann MD, Stuhlmeier KM, Mascher DG, Nowotny PN, Zlabinger GJ, Waldhausl W and Stulnig TM 
In:
J Biol Chem (2005) 280(14): 14293-14301 
Categories:
Transfection 
Authors:
Riol-Blanco L, Sanchez-Sanchez N, Torres A, Tejedor A, Narumiya S, Corbi AL, Sanchez-Mateos P and Rodriguez-Fernandez JL 
In:
J Immunol (2005) 174(7): 4070-4080 
Categories:
Transfection 
Authors:
Wirtz S, Becker C, Fantini MC, Nieuwenhuis EE, Tubbe I, Galle PR, Schild HJ, Birkenbach M, Blumberg RS and Neurath MF 
In:
J Immunol (2005) 174(5): 2814-2824 
Categories:
Transfection 
Authors:
Mincheff M, Zoubak S, Altankova I, Tchakarov S, Pogribnyy P, Makogonenko Y, Botev C and Meryman HT 
In:
Cancer Gene Ther (2005) 12(2): 185-197 
Categories:
Transfection 
Authors:
Mandic M, Castelli F, Janjic B, Almunia C, Andrade P, Gillet D, Brusic V, Kirkwood JM, Maillere B and Zarour HM 
In:
J Immunol (2005) 174(3): 1751-1759 
Categories:
Transfection 
Authors:
Arrieumerlou C and Meyer T 
In:
Dev Cell (2005) 8(2): 215-227 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Brandler S, Brown N, Ermak TH, Mitchell F, Parsons M, Zhang Z, Lang J, Monath TP, Guirakhoo F. 
In:
Am. J. Trop. Med (2005) 72(1): 74-81 
Categories:
Transfection 
Authors:
Sanchez-Sanchez N, Riol-Blanco L, de la Rosa G, Puig-Kroger A, Garcia-Bordas J, Martin D, Longo N, Cuadrado A, Cabanas C, Corbi AL, Sanchez-Mateos P and Rodriguez-Fernandez JL 
In:
Blood (2004) 104(3): 619-625 
Categories:
Transfection 
Authors:
Shi GX, Harrison K, Han SB, Moratz C and Kehrl JH 
In:
J Immunol (2004) 172(9): 5175-5184 
Categories:
Transfection 
Authors:
Nagaraj S, Ziske C and Schmidt-Wolf IGH 
In:
Indian J Med Res (2004) 119: 133-138 
Categories:
Primary Cells and Media 
Authors:
Mercier GT, Campbell JA, Chappell JD, Stehle T, Dermody TS, Barry MA. 
In:
Proc Natl Acad Sci USA (2004) 101(6): 6188-93 
Categories:
Transfection 
Authors:
Coughlin CM, Vance BA, Grupp SA and Vonderheide RH 
In:
Blood (2004) 103: 2046-2054 
Categories:
Primary Cells and Media 
Authors:
Schauber CA, Tuerk MJ, Pacheco CD, Escarpe PA, Veres G. 
In:
Gene Ther (2004) 11(3): 266-75 
Categories:
Transfection 
Authors:
Beinhauer BG, McBride JM, Graf P, Pursch E, Bongers M, Rogy M, Korthauer U, de Vries JE, Aversa G and Jung T 
In:
Eur J Immunol (2004) 34: 74-80 
Categories:
Transfection 
Authors:
Mincheff M, Zoubak S, Altankova I, Tchakarov S, Makogonenko Y, Botev C, Ignatova I, Dimitrov R, Madarzhieva K, Hammett M, Pomakov Y, Meryman H and Lissitchkov T 
In:
Cancer Gene Ther (2003) 10: 907-917 
Categories:
Transfection 
Authors:
Bros M, Ross XL, Pautz A, Reske-Kunz AB and Ross R 
In:
J Immunol (2003) 171: 1825-1834 
Categories:
Transfection 
Authors:
Lenz P, Bacot SM, Frazier-Jessen MR and Feldman GM 
In:
FEBS Lett (2003) 538(1-3): 149-154 
Categories:
Transfection 
Authors:
Mordmüller B, Krappmann D, Esen M, Wegener E and Scheidereit C 
In:
EMBO Rep (2003) 4(1): 82-87 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yang D, Chen Q, Chertov O, Oppenheim JJ. 
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J Leukoc Biol (2000) 68(1): 9-14 
Categories:
Primary Cells and Media 
Authors:
Li C1,2, Bi Y3, Li Y1,2, Yang H2, Yu Q1, Wang J1,2, Wang Y1,2, Su H1,2, Jia A1, Hu Y1, Han L1, Zhang J1, Li S1, Tao W4, Liu G1,2 
In:
Nat Commun. () :  
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