Dendritic cell (NHDC), human

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Immature or mature dendritic cells; monocyte-derived. DCs are antigen presenting cells for T cells and are typically found in the skin and mucosal membranes.

Cell Type:
Dendritic cell (DC)
Tissue Origin:
blood
Species:
human
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Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Lymphocyte Growth Medium-3
LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
DC, human U-002 36-62% 71% Plasmid (general) 5 µg 100 µl I/II/2b
P3 CB-150 4e5 57-81% 82-86% mRNA 0.5 µg 20 µl 4D X-Unit
P3 CB-150 4e5 42-66% 79-89% mRNA 0.5 µg 20 µl 4D X-Unit
P3 CB-150 4e5 33-57% 77-81% mRNA 0.5 µg 20 µl 4D X-Unit
P3 EH-104 4e5 82-84% 51-57% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 CB-150 2e5 28-36% 96-98% mRNA 0.5 µg 20 µl 4D X-Unit
P3 CB-150 2e5 17-23% 96-98% mRNA 0.5 µg 20 µl 4D X-Unit
P3 EH-104 2e5 87% 84% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 EH-104 2e5 93% 71-81% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (59)

CRISPR-based functional genomics in human dendritic cells
Categories:
Transfection 
Authors:
Marco Jost, Amy N. Jacobson, Jeffrey A. Hussmann, Giana Cirolia,  Michael A. Fischbach,  Jonathan S. Weissman 
In:
BioResearch Open Access (2020) 1: 1-76 
Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9.
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Freund EC, Lock JY, Oh J, Maculins T, Delamarre L, Bohlen CJ, Haley B, Murthy A. 
In:
J Exp Med (2020) 217(7): e20191692 
Fast-track development of an in vitro 3D lung/immune cell model to study Aspergillus infections.
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Chandorkar P, Posch W, Zaderer V, Blatzer M, Steger M, Ammann CG, Binder U, Hermann M, Hörtnagl P, Lass-Flörl C, Wilflingseder D 
In:
Scientific Reports (2017) 7(1): 10.1038/s41598-017-11271-4 
Going non-viral: the Sleeping Beauty transposon system breaks on through to the clinical side.
Categories:
Transfection 
Authors:
Hudecek M1, Izsvák Z2, Johnen S3, Renner M4, Thumann G5, Ivics Z4. 
In:
Clin Exp Immunol (2017) 52(4): 355(80) 
Mincle Activation and the Syk/Card9 Signaling Axis are Central to the Development of the Autoimmune Disease of the Eye
Categories:
Serum-free and Speciality Media 
Authors:
Ellen J. Lee, Brieanna R. Brown, Emily E. Vance, Paige E. Snow, Phyllis B. Silver, David Heinrichs, Xin Lin, Yoichiro Iwakura, Christine A. Wells, Rachel R. Caspi and Holly L. Rosenzweig 
In:
J Immunol (2016) 196: 7 
Dendritic cell SIRT1–HIF1a axis programs the differentiation of CD4+ T cells through IL-12 and TGF-ß1
Categories:
Primary Cells and Media 
Authors:
Liu G1, Bi Y2, Xue L3, Zhang Y4, Yang H4, Chen X4, Lu Y4, Zhang Z4, Liu H4, Wang X4, Wang R5, Chu Y4, Yang R2. 
In:
Proc Natl Acad Sci USA (2015) 112(9): E957-65 
HBD3 regulates matrix metalloproteinase production in human myeloid dendritic cells exposed to Porphyromonas gingivalis hemagglutinin B
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
Authors:
Raina M 
In:
Other (2014) : Thesis 
Differential cytotoxicity of long-chain bases for human oral keratinocytes, fibroblasts, dendritic and oral squamous cell carcinoma cell lines
Categories:
 
Authors:
Poulsen C 
In:
Other (2014) 0:0: Thesis 
c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Micheva-Viteva SN, Shou Y, Nowak-Lovato KL, Rector KD, Hong-Geller E 
In:
BMC Microbiol. (2013) 13: 249 
Sphingoid bases induce dose-dependent cytotoxicity and cytokine responses in human myeloid dendritic cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mehalick LA 
In:
Other (2013) 0:0: Thesis 
Defensin DEFB103 bidirectionally regulates chemokine and cytokine responses to a pro-inflammatory stimulus.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
Authors:
Harvey LE, Kohlgraf KG, Mehalick LA, Raina M, Recker EN, Radhakrishnan S, Prasad SA, Vidva R, Progulske-Fox A, Cavanaugh JE, Vali S, Brogden KA 
In:
Other (2013) 3: 1232 
Development of an Alternative Test for Skin Sensitization Using a Three-dimensional Human Skin Model Consisting of Dendritic Cells, Keratinocytes and Fibroblasts .
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uchino T, Takezawa T, Ikarashi Y, Nishimura T 
In:
AATEX (2011) 16(1): 1-8 
Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant.
Categories:
Transfection 
Authors:
Gerdemann U, Vera JF, Rooney CM, Leen AM. 
In:
J Vis Exp (2011) 51: 2736 
Histatin 5 attenuates IL-8 dendritic cell response to gingivalis Hemagglutinin B
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Borgwardt DS 
In:
Other (2011) 0:0: Thesis 
Nucleofection of DCs to generate Multivirus-specific T cells for prevention or treatment of viral infections in the immunocompromised host.
Categories:
Transfection 
Authors:
Gerdemann U, Christin AS, Vera JF, Ramos CA, Fujita Y, Liu H, Dilloo D, Heslop HE, Brenner MK, Rooney CM, Leen AM. 
In:
Mol Ther (2009) 17(9): 1616-25 
The MHC class II-associated invariant chain interacts with the neonatal Fc gamma receptor and modulates its trafficking to endosomal/lysosomal compartments
Categories:
Transfection 
Authors:
Ye L, Liu X, Rout SN, Li Z, Yan Y, Lu L, Kamala T, Nanda NK, Song W, Samal SK, Zhu X 
In:
J Immunol (2008) 181(4): 2572-85 
APOBEC3G upregulation by alpha interferon restricts human immunodeficiency virus type 1 infection in human peripheral plasmacytoid dendritic cells
Categories:
Transfection 
Authors:
Wang FX, Huang J, Zhang H, Ma X, Zhang H 
In:
J Gen Virol (2008) 89(Pt 3): 722-30 
Regulation of CD1a surface expression and antigen presentation by invariant chain and lipid rafts
Categories:
Transfection 
Authors:
Sloma I, Zilber MT, Vasselon T, Setterblad N, Cavallari M, Mori L, De Libero G, Charron D, Mooney N, Gelin C 
In:
J Immunol (2008) 180(2): 980-7 
Syndecan-3 is a dendritic cell-specific attachment receptor for HIV-1
Categories:
Transfection 
Authors:
de Witte L, Bobardt M, Chatterji U, Degeest G, David G, Geijtenbeek TB, Gallay P 
In:
Proc Natl Acad Sci USA (2007) 104(49): 19464-9 
High transfection efficiency, gene expression, and viability of monocyte-derived human dendritic cells after nonviral gene transfer
Categories:
Transfection 
Authors:
Landi A, Babiuk LA, van Drunen Littel-van den Hurk S 
In:
J Leukoc Biol (2007) 82(4): 849-60 
Characterization of human immunodeficiency virus type 1 replication in immature and mature dendritic cells reveals dissociable cis- and trans-infection
Categories:
Transfection 
Authors:
Dong C, Janas AM, Wang JH, Olson WJ, Wu L 
In:
J Virol (2007) 81(20): 11352-62 
Generation and maturation of bone marrow-derived DCs under serum-free conditions
Categories:
Serum-free and Speciality Media 
Authors:
Sung Jung Kim, Betty Diamond 
In:
J Immunol Methods (2007) 323: 2007 
Construction of a Three-Dimensional Human Skin Model Consisting of Keratinocytes, Dendritic Cells and Fibroblasts and Application of This Model for Alternative Animal Testing of Immune-Sensitizing Compounds
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Uchino T, Ikarashi Y, Tokunaga H 
In:
Other (2007) 41(4): 246-253 
Robust CD4(+) and CD8(+) T cell responses to SIV using mRNA-transfected DC expressing autologous viral Ag
Categories:
Transfection 
Authors:
Melhem NM, Liu XD, Boczkowski D, Gilboa E, Barratt-Boyes SM 
In:
Eur J Immunol (2007) 37(8): 2164-73 
Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper
Categories:
Transfection 
Authors:
Hamdi H, Godot V, Maillot MC, Prejean MV, Cohen N, Krzysiek R, Lemoine FM, Zou W, Emilie D 
In:
Blood (2007) 110(1): 211-9 
Liver X receptors regulate dendritic cell phenotype and function through blocked induction of the actin-bundling protein fascin
Categories:
Transfection 
Authors:
Geyeregger R, Zeyda M, Bauer W, Kriehuber E, Saemann MD, Zlabinger GJ, Maurer D, Stulnig TM 
In:
Blood (2007) 109(10): 4288-95 
Sensitization to TLR7 Agonist in IFN-beta-Preactivated Dendritic Cells
Categories:
Transfection 
Authors:
Severa M, Remoli ME, Giacomini E, Annibali V, Gafa V, Lande R, Tomai M, Salvetti M, Coccia EM 
In:
J Immunol (2007) 178(10): 6208-16 
Vaccination with NY-ESO-1 protein and CpG in Montanide induces integrated antibody/Th1 responses and CD8 T cells through cross-priming
Categories:
Transfection 
Authors:
Valmori D, Souleimanian NE, Tosello V, Bhardwaj N, Adams S, O'neill D, Pavlick A, Escalon JB, Cruz CM, Angiulli A, Angiulli F, Mears G, Vogel SM, Pan L, Jungbluth AA, Hoffmann EW, Venhaus R, Ritter G, Old LJ, Ayyoub M 
In:
Proc Natl Acad Sci USA (2007) 104(21): 8947-52 
Disparity in IL-12 Release in Dendritic Cells and Macrophages in Response to Mycobacterium tuberculosis Is Due to Use of Distinct TLRs
Categories:
Transfection 
Authors:
Pompei L, Jang S, Zamlynny B, Ravikumar S, McBride A, Hickman SP, Salgame P 
In:
J Immunol (2007) 178(8): 5192-9 
NF-kappaB is required for STAT-4 expression during dendritic cell maturation
Categories:
Transfection 
Authors:
Remoli ME, Ragimbeau J, Giacomini E, Gafa V, Severa M, Lande R, Pellegrini S, Coccia EM 
In:
J Leukoc Biol (2007) 81(1): 355-63 
The Wild-Type Sequence (wt) p5325-35 Peptide Induces HLA-DR7 and HLA-DR11-Restricted CD4+ Th Cells Capable of Enhancing the Ex Vivo Expansion and Function of Anti-wt p53264-272 Peptide CD8+ T Cells
Categories:
Transfection 
Authors:
Ito D, Albers A, Zhao YX, Visus C, Appella E, Whiteside TL, Deleo AB 
In:
J Immunol (2006) 177(10): 6795-803 
Human T-cell responses to vaccinia virus envelope proteins
Categories:
Transfection 
Authors:
Tang J, Murtadha M, Schnell M, Eisenlohr LC, Hooper J, Flomenberg P 
In:
J Virol (2006) 80(20): 10010-20 
A Critical Role for Prostaglandin E2 in Podosome Dissolution and Induction of High-Speed Migration during Dendritic Cell Maturation
Categories:
Transfection 
Authors:
van Helden SF, Krooshoop DJ, Broers KC, Raymakers RA, Figdor CG, van Leeuwen FN 
In:
J Immunol (2006) 177(3): 1567-74 
Small Interfering RNA-Mediated Knockdown of Notch Ligands in Primary CD4+ T Cells and Dendritic Cells Enhances Cytokine Production
Categories:
Transfection 
Authors:
Stallwood Y, Briend E, Ray KM, Ward GA, Smith BJ, Nye E, Champion BR, McKenzie GJ 
In:
J Immunol (2006) 177(2): 885-95 
Processing of Tumor-Associated Antigen by the Proteasomes of Dendritic Cells Controls In vivo T-Cell Responses
Categories:
Transfection 
Authors:
Chapatte L, Ayyoub M, Morel S, Peitrequin AL, Levy N, Servis C, Van den Eynde BJ, Valmori D, Levy F 
In:
Cancer Res (2006) 66(10): 5461-8 
SLAM/SLAM interactions inhibit CD40-induced production of inflammatory cytokines in monocyte derived dendritic cells
Categories:
Transfection 
Authors:
Rethi B, Gogolak P, Szatmari I, Veres A, Erdos E, Nagy L, Rajnavolgyi E, Terhorst C, Lanyi A 
In:
Blood (2006) 107(7): 2821-9 
The transcription factor GATA-1 potently represses the expression of the HIV-1 co-receptor CCR5 in human T cells and dendritic cells
Categories:
Transfection 
Authors:
Sundrud MS, Vancompernolle SE, Eger KA, Bruno TC, Subramaniam A, Mummidi S, Ahuja SK and Unutmaz D 
In:
Blood (2005) 106(10): 3440-3448 
Differential effect of agonistic anti-CD40 on human mature and immature dendritic cells: the Janus face of anti-CD40
Categories:
Transfection 
Authors:
de Goer de Herve MG, Durali D, Tran TA, Maigne G, Simonetta F, Leclerc P, Delfraissy JF and Taoufik Y 
In:
Blood (2005) 106(8): 2806-2814 
Restoration by IL-15 of MHC Class I Antigen-Processing Machinery in Human Dendritic Cells Inhibited by Tumor-Derived Gangliosides
Categories:
Transfection 
Authors:
Tourkova IL, Shurin GV, Chatta GS, Perez L, Finke J, Whiteside TL, Ferrone S and Shurin MR 
In:
J Immunol (2005) 175(5): 3045-3052 
Polyunsaturated fatty acids block dendritic cell activation and function independently of NF-B activation
Categories:
Transfection 
Authors:
Zeyda M, Saemann MD, Stuhlmeier KM, Mascher DG, Nowotny PN, Zlabinger GJ, Waldhausl W and Stulnig TM 
In:
J Biol Chem (2005) 280(14): 14293-14301 
The Chemokine Receptor CCR7 Activates in Dendritic Cells Two Signaling Modules That Independently Regulate Chemotaxis and Migratory Speed
Categories:
Transfection 
Authors:
Riol-Blanco L, Sanchez-Sanchez N, Torres A, Tejedor A, Narumiya S, Corbi AL, Sanchez-Mateos P and Rodriguez-Fernandez JL 
In:
J Immunol (2005) 174(7): 4070-4080 
EBV-Induced Gene 3 Transcription Is Induced by TLR Signaling in Primary Dendritic Cells via NF-(kappa)B Activation
Categories:
Transfection 
Authors:
Wirtz S, Becker C, Fantini MC, Nieuwenhuis EE, Tubbe I, Galle PR, Schild HJ, Birkenbach M, Blumberg RS and Neurath MF 
In:
J Immunol (2005) 174(5): 2814-2824 
Depletion of CD25(+) cells from human T-cell enriched fraction eliminates immunodominance during priming with dendritic cells genetically modified to express a secreted protein
Categories:
Transfection 
Authors:
Mincheff M, Zoubak S, Altankova I, Tchakarov S, Pogribnyy P, Makogonenko Y, Botev C and Meryman HT 
In:
Cancer Gene Ther (2005) 12(2): 185-197 
One NY-ESO-1-Derived Epitope That Promiscuously Binds to Multiple HLA-DR and HLA-DP4 Molecules and Stimulates Autologous CD4+ T Cells from Patients with NY-ESO-1-Expressing Melanoma
Categories:
Transfection 
Authors:
Mandic M, Castelli F, Janjic B, Almunia C, Andrade P, Gillet D, Brusic V, Kirkwood JM, Maillere B and Zarour HM 
In:
J Immunol (2005) 174(3): 1751-1759 
A local coupling model and compass parameter for eukaryotic chemotaxis
Categories:
Transfection 
Authors:
Arrieumerlou C and Meyer T 
In:
Dev Cell (2005) 8(2): 215-227 
Replication of chimeric yellow fever virus-dengue serotype 1-4 virus vaccine strains in dendritic and hepatic cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Brandler S, Brown N, Ermak TH, Mitchell F, Parsons M, Zhang Z, Lang J, Monath TP, Guirakhoo F. 
In:
Am. J. Trop. Med (2005) 72(1): 74-81 
Chemokine receptor CCR7 induces intracellular signaling that inhibits apoptosis of mature dendritic cells
Categories:
Transfection 
Authors:
Sanchez-Sanchez N, Riol-Blanco L, de la Rosa G, Puig-Kroger A, Garcia-Bordas J, Martin D, Longo N, Cuadrado A, Cabanas C, Corbi AL, Sanchez-Mateos P and Rodriguez-Fernandez JL 
In:
Blood (2004) 104(3): 619-625 
Toll-like receptor signaling alters the expression of regulator of g protein signaling proteins in dendritic cells: implications for g protein-coupled receptor signaling
Categories:
Transfection 
Authors:
Shi GX, Harrison K, Han SB, Moratz C and Kehrl JH 
In:
J Immunol (2004) 172(9): 5175-5184 
Dendritic cell, the immunotherapeutic cell for cancer
Categories:
Transfection 
Authors:
Nagaraj S, Ziske C and Schmidt-Wolf IGH 
In:
Indian J Med Res (2004) 119: 133-138 
A chimeric adenovirus vector encoding reovirus attachment protein sigma1 targets cells expressing junctional adhesion molecule 1
Categories:
Primary Cells and Media 
Authors:
Mercier GT, Campbell JA, Chappell JD, Stehle T, Dermody TS, Barry MA. 
In:
Proc Natl Acad Sci USA (2004) 101(6): 6188-93 
RNA-transfected CD40-activated B cells induce functional T-cell responses against viral and tumor antigen targets: implications for pediatric immunotherapy
Categories:
Transfection 
Authors:
Coughlin CM, Vance BA, Grupp SA and Vonderheide RH 
In:
Blood (2004) 103: 2046-2054 
Lentiviral vectors pseudotyped with baculovirus gp64 efficiently transduce mouse cells in vivo and show tropism restriction against hematopoietic cell types in vitro
Categories:
Primary Cells and Media 
Authors:
Schauber CA, Tuerk MJ, Pacheco CD, Escarpe PA, Veres G. 
In:
Gene Ther (2004) 11(3): 266-75 
Interleukin 10 regulates cell surface and soluble LIR-2 (CD85d) expression on dendritic cells resulting in T cell hyporesponsiveness in vitro
Categories:
Transfection 
Authors:
Beinhauer BG, McBride JM, Graf P, Pursch E, Bongers M, Rogy M, Korthauer U, de Vries JE, Aversa G and Jung T 
In:
Eur J Immunol (2004) 34: 74-80 
Human dendritic cells genetically engineered to express cytosolically retained fragment of prostate-specific membrane antigen prime cytotoxic T-cell responses to multiple epitopes
Categories:
Transfection 
Authors:
Mincheff M, Zoubak S, Altankova I, Tchakarov S, Makogonenko Y, Botev C, Ignatova I, Dimitrov R, Madarzhieva K, Hammett M, Pomakov Y, Meryman H and Lissitchkov T 
In:
Cancer Gene Ther (2003) 10: 907-917 
The human fascin gene promoter is highly active in mature dendritic cells due to a stage-specific enhancer
Categories:
Transfection 
Authors:
Bros M, Ross XL, Pautz A, Reske-Kunz AB and Ross R 
In:
J Immunol (2003) 171: 1825-1834 
Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells
Categories:
Transfection 
Authors:
Lenz P, Bacot SM, Frazier-Jessen MR and Feldman GM 
In:
FEBS Lett (2003) 538(1-3): 149-154 
Lymphotoxin and lipopolysaccharide induce NF-kappaB-p52 generation by a co-translational mechanism
Categories:
Transfection 
Authors:
Mordmüller B, Krappmann D, Esen M, Wegener E and Scheidereit C 
In:
EMBO Rep (2003) 4(1): 82-87 
Human neutrophil defensins selectively chemoattract naive T and immature dendritic cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yang D, Chen Q, Chertov O, Oppenheim JJ. 
In:
J Leukoc Biol (2000) 68(1): 9-14 
Dendritic cell MST1 inhibits Th17 differentiation.
Categories:
Primary Cells and Media 
Authors:
Li C1,2, Bi Y3, Li Y1,2, Yang H2, Yu Q1, Wang J1,2, Wang Y1,2, Su H1,2, Jia A1, Hu Y1, Han L1, Zhang J1, Li S1, Tao W4, Liu G1,2 
In:
Nat Commun. () :  
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