Human neutrophil defensins selectively chemoattract naive T and immature dendritic cells.

Authors:
Yang D, Chen Q, Chertov O, Oppenheim JJ.
In:
Source: J Leukoc Biol
Publication Date: (2000)
Issue: 68(1): 9-14
Research Area:
Basic Research
Cells used in publication:
Dendritic cell (NHDC), human
Species: human
Tissue Origin: blood
CD34+ cell, human
Species: human
Tissue Origin: blood
Monocyte, human
Species: human
Tissue Origin: blood
T cell, human cord blood unstim.
Species: human
Tissue Origin: blood
Culture Media:
Abstract
Defensins, a family of cationic, structurally related, antimicrobial peptides, contribute to host defense by disrupting the cytoplasmic membrane of microbes. Here we show that human neutrophil defensins selectively induce the migration of human CD4+/CD45RA+ naive and CD8+, but not CD4+/CD45RO+ memory, T cells. Moreover, human neutrophil defensins are chemotactic for immature human dendritic cells derived from either CD34+ progenitors or peripheral blood monocytes. Upon maturation induced by treatment with tumor necrosis factor alpha (TNF-alpha), dendritic cells lose their responsiveness to human neutrophil defensins. The chemotactic effect of human neutrophil defensins on both T and dendritic cells is pertussis toxin-sensitive, suggesting that a G(ialpha) protein-coupled receptor is responsible. Human neutrophil defensins are also chemotactic for immature murine dendritic cells. These data suggest that, in addition to their antimicrobial role, human neutrophil defensins also contribute to adaptive immunity by mobilizing T cells and dendritic cells.