T cell, human cord blood unstim.

Cord Blood CD4+ T cells which express the cell surface marker CD45RA are defined as naive and are deficient in their ability to assist in antibody production. Such cells are particularly abundant in human cord blood3. CD4+ T cells are commonly used to produce Th0, Th1 and Th2 T cell populations. The Cord Blood CD4+ T cells are isolated from cord blood mononuclear cells using negative immunomagnetic selection.

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and human or recombinant transferrin.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations (7)

Categories:
Primary Cells and Media, Transfection 
Authors:
Farboud B1, Jarvis E2, Roth TL3, Shin J4, Corn JE4, Marson A5, Meyer BJ1, Patel NH6, Hochstrasser ML7. 
In:
J Vis Exp (2018) 2018: 135 
Categories:
Transfection 
Authors:
Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H. 
In:
Frontiers in Immunology (2017) 1: 1-9 
Categories:
Transfection 
Authors:
Prabha Chandrasekaran, Victoria Moore, Monica Buckley, Joshua Spurrier, John H. Kehrl, Sundararajan Venkatesan 
In:
PLoS ONE (2014) 9(1): e86998 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Isgro J, Gupta S, Jacek E, Pavri T, Duculan R, Kim M, Kirou KA, Salmon JE, Pernis AB 
In:
Arthritis Rheum (2013) 65(6): 1592-602 
Categories:
Transfection 
Authors:
Singh H, Figliola MJ, Dawson MJ, Olivares S, Zhang L, Yang G, Maiti S, Manuri P, Senyukov V, Jena B, Kebriaei P, Champlin RE, Huls H, Cooper LJ. 
In:
PLoS ONE (2013) 8(5): 64138 
Categories:
Primary Cells and Media 
Authors:
Votavova H, Grmanova M, Dostalova Merkerova M, Belickova M, Vasikova A, Neuwirtova R, Cermak J 
In:
J Hematol Oncol (2011) 6(4): 1 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yang D, Chen Q, Chertov O, Oppenheim JJ. 
In:
J Leukoc Biol (2000) 68(1): 9-14 
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