Mononuclear, peripheral blood, human

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Human Peripheral Blood Mononuclear Cells

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
blood
Species:
human
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Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and human or recombinant transferrin.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

HL-1

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

Lymphocyte Growth Medium-3
LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations (39)

Co-culture of outgrowth endothelial cells with human mesenchymal stem cells in silk fibroin hydrogels promotes angiogenesis.
Categories:
 
Authors:
Sun W, Motta A, Shi Y, Seekamp A, Schmidt H, Gorb SN, Migliaresi C, Fuchs S. 
In:
Biomaterials (2016) 11(3): doi: 10.1088/1748-6041/11/3/035009 
Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Cantoni S, Bianchi F, Galletti M, Olivi E, Alviano F, Galiè N, Ventura C 
In:
Stem Cells (2015) 2015: 943671 
Mechanisms of vasculogenesis in 3D fibrin matrices mediated by the interaction of adipose-derived stem cells and endothelial cells
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rohringer S, Hofbauer P, Schneider KH, Husa AM, Feichtinger G, Peterbauer-Scherb A, Redl H, Holnthoner W. 
In:
Angiogenesis (2014) 17(4): 921-33 
Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Masuda H, Tanaka R, Fujimura S, Ishikawa M, Akimaru H, Shizuno T, Sato A, Okada Y, Iida Y, Itoh J, Itoh Y, Kamiguchi H, Kawamoto A, Asahara T 
In:
J Am Heart Assoc (2014) 3(3): e000743 
Linkage between dendritic and T cell commitments in human circulating hematopoietic progenitors.
Categories:
Primary Cells and Media 
Authors:
Kyoizumi S, Kubo Y, Kajimura J, Yoshida K, Hayashi T, Nakachi K, Young LF, Moore MA, van den Brink MR, Kusunoki Y 
In:
J Immunol (2014) 192(12): 5749-60 
Advanced Glycation End Products (AGEs) Cause Pro-atherogenic Changes in Cholesterol Transport: A Possible Mechanism for Cardiovascular Risk in Diabetes
Categories:
Primary Cells and Media 
Authors:
Gamez, JD, Voloshyna I, Littlefield MJ, Castro-Magana M, Reiss AB 
In:
Other (2014) S11:005: ePub 
HBD3 regulates matrix metalloproteinase production in human myeloid dendritic cells exposed to Porphyromonas gingivalis hemagglutinin B
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
Authors:
Raina M 
In:
Other (2014) : Thesis 
Superior Potential of CD34-Positive Cells Compared to Total Mononuclear Cells for Healing of Nonunion Following Bone Fracture.
Categories:
Primary Cells and Media 
Authors:
Fukui T, Mifune Y, Matsumoto T, Shoji T, Kawakami Y, Kawamoto A, Ii M, Akimaru H, Kuroda T, Horii M, Yokoyama A, Alev C, Kuroda R, Kurosaka M, Asahara T 
In:
Cell Transplant (2014) ePub: ePub 
CD4 T cells require ICOS-mediated PI3K signaling to increase T-Bet expression in the setting of anti-CTLA-4 therapy.
Categories:
Transfection 
Authors:
Chen H1, Fu T, Suh WK, Tsavachidou D, Wen S, Gao J, Ng Tang D, He Q, Sun J, Sharma P. 
In:
Cancer Immunol Immunother (2014) 2(2): 167-76. 
Advanced Glycation End Products Promote Pro-Atherogenic Changes in Cholesterol Transport: A Possible Mechanism for Cardiovascular Risk in Diabetes
Categories:
Primary Cells and Media 
Authors:
Voloshyna I, Gamez-Godoy J, Littlefield MJ, De Leon J, Castro-Magana M, Reiss AB 
In:
Other (2014) ePub: ePub 
Association study of circulating endothelial progenitor cells and plasma prostacyclin levels in pulmonary hypertension rats.
Categories:
 
Authors:
Song Y 
In:
ACS Nano (2014) 12(1): 438-44 
A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lee JH, Sohn JH, Ryu SY, Hong CS, Moon KD, Park JW 
In:
J Cell Mol Med (2013) 17(10): 1271-81 
Age-associated changes in the differentiation potentials of human circulating hematopoietic progenitors to T- or NK-lineage cells
Categories:
Primary Cells and Media 
Authors:
Kyoizumi S, Kubo Y, Kajimura J, Yoshida K, Imai K, Hayashi T, Nakachi K, Young LF, Moore MA, van den Brink MR, Kusunoki Y. 
In:
J Immunol (2013) 190(12): 6164-72 
Sphingoid bases induce dose-dependent cytotoxicity and cytokine responses in human myeloid dendritic cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mehalick LA 
In:
Other (2013) 0:0: Thesis 
Defensin DEFB103 bidirectionally regulates chemokine and cytokine responses to a pro-inflammatory stimulus.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Endotoxin Detection 
Authors:
Harvey LE, Kohlgraf KG, Mehalick LA, Raina M, Recker EN, Radhakrishnan S, Prasad SA, Vidva R, Progulske-Fox A, Cavanaugh JE, Vali S, Brogden KA 
In:
Other (2013) 3: 1232 
Preclinical safety and efficacy of human CD34(+) cells transduced with lentiviral vector for the treatment of Wiskott-Aldrich syndrome.
Categories:
Primary Cells and Media 
Authors:
Scaramuzza S, Biasco L, Ripamonti A, Castiello MC, Loperfido M, Draghici E, Hernandez RJ, Benedicenti F, Radrizzani M, Salomoni M, Ranzani M, Bartholomae CC, Vicenzi E, Finocchi A, Bredius R, Bosticardo M, Schmidt M, von Kalle C, Montini E, Biffi A, Roncarolo MG, Naldini L, Villa A, Aiuti A 
In:
Mol Ther (2013) 21(1): 175-84 
Local Transplantation of Granulocyte Colony-Stimulating Factor-Mobilized Human Peripheral Blood Mononuclear Cells for Unhealing Bone Fractures
Categories:
Primary Cells and Media 
Authors:
Fukui T, Matsumoto T, Mifune Y, Shoji T, Kuroda T, Kawakami Y, Kawamoto A, Ii M, Kawamata S, Kurosaka M, Asahara T, Kuroda R. 
In:
Cell Transplant (2012) 21: 707-721 
Resveratrol ameliorates TNFa-mediated suppression of erythropoiesis in human CD34(+) cells via modulation of NF-?B signalling.
Categories:
Primary Cells and Media 
Authors:
Jeong JY, Silver M, Parnes A, Nikiforow S, Berliner N, Vanasse GJ. 
In:
Br J of Haematol (2011) 155(1): 93-101 
Wnt1 is a proangiogenic molecule, enhances human endothelial progenitor function, and increases blood flow to ischemic limbs in a HGF-dependent manner.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gherghe CM, Duan J, Gong J, Rojas M, Klauber-Demore N, Majesky M, Deb A. 
In:
FASEB J (2011) 25(6): 1836-43 
COMP-Ang1 stimulates HIF-1a-mediated SDF-1 overexpression and recovers ischemic injury through BM-derived progenitor cell recruitment.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Youn SW, Lee SW, Lee J, Jeong HK, Suh JW, Yoon CH, Kang HJ, Kim HZ, Koh GY, Oh BH, Park YB, Kim HS. 
In:
Blood (2011) 117(16): 4376-86 
Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures.
Categories:
Transfection 
Authors:
Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA, Tong J, Cheng L. 
In:
Cell Res (2011) 21: 518-529 
Adaptive mutations in the genomes of enterovirus 71 strains following infection of mouse cells expressing human P-selectin glycoprotein ligand-1.
Categories:
Primary Cells and Media 
Authors:
Miyamura K, Nishimura Y, Abo M, Wakita T, Shimizu H 
In:
J Gen Virol (2011) 92(Pt2): 287-91 
Expression pattern of the thioredoxin system in human endothelial progenitor cells and endothelial cells under hypoxic injury.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Park KJ, Kim YJ, Choi EJ, Park NK, Kim GH, Kim SM, Lee SY, Bae JW, Hwang KK, Kim DW, Cho MC. 
In:
Other (2010) 40(12): 651-658 
MicroRNA-21 and microRNA-148a contribute to DNA hypomethylation in lupus CD4+ T cells by directly and indirectly targeting DNA methyltransferase 1.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Pan W, Zhu S, Yuan M, Cui H, Wang L, Luo X, Li J, Zhou H, Tang Y, Shen N. 
In:
J Immunol (2010) 184(12): 6773-81 
The plant hormone abscisic acid stimulates the proliferation of human hemopoietic progenitors through the second messenger cyclic ADP-ribose.
Categories:
Primary Cells and Media 
Authors:
Scarfì S, Fresia C, Ferraris C, Bruzzone S, Fruscione F, Usai C, Benvenuto F, Magnone M, Podestà M, Sturla L, Guida L, Albanesi E, Damonte G, Salis A, De Flora A, Zocchi E. 
In:
Stem Cells (2009) 27(10): 2469-77 
Release of proinflammatory mediators and expression of proinflammatory adhesion molecules by endothelial progenitor cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Zhang Y, Ingram DA, Murphy MP, Saadatzadeh MR, Mead LE, Prater DN, Rehman J. 
In:
Am J Physiol Heart Circ Physiol (2009) 296(5): H1675-82 
Acceleration of skeletal muscle regeneration in a rat skeletal muscle injury model by local injection of human peripheral blood-derived CD133-positive cells.
Categories:
Primary Cells and Media 
Authors:
Shi M, Ishikawa M, Kamei N, Nakasa T, Adachi N, Deie M, Asahara T, Ochi M. 
In:
Stem Cells (2009) 27(4): 949-60 
O2 level controls hematopoietic circulating progenitor cells differentiation into endothelial or smooth muscle cells.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Berthelemy N, Kerdjoudj H, Schaaf P, Prin-Mathieu C, Lacolley P, Stoltz JF, Voegel JC, Menu P. 
In:
PLoS ONE (2009) 4(5): e5514 
Retrovirally induced CTL degranulation mediated by IL-15 expression and infection of mononuclear phagocytes in patients with HTLV-I-associated neurologic disease.
Categories:
Primary Cells and Media 
Authors:
Enose-Akahata Y, Oh U, Grant C, Jacobson S. 
In:
Blood (2008) 112(6): 2400-10 
Administrations of peripheral blood CD34-positive cells contribute to medial collateral ligament healing via vasculogenesis.
Categories:
Primary Cells and Media 
Authors:
Tei K, Matsumoto T, Mifune Y, Ishida K, Sasaki K, Shoji T, Kubo S, Kawamoto A, Asahara T, Kurosaka M, Kuroda R 
In:
Stem Cells (2008) 26(3): 819-30 
NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A. 
In:
Blood (2008) 111(3): 1428-36 
Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity.
Categories:
Primary Cells and Media 
Authors:
Povsic TJ, Zavodni KL, Kelly FL, Zhu S, Goldschmidt-Clermont PJ, Dong C, Peterson ED. 
In:
J Am Coll Cardiol (2007) 50(23): 2243-8 
Tyrosine kinase Syk associates with toll-like receptor 4 and regulates signaling in human monocytic cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chaudhary A, Fresquez TM, Naranjo MJ. 
In:
Immunol Cell Biol (2007) 85(3): 249-56 
Extracellular Nef protein targets CD4+ T cells for apoptosis by interacting with CXCR4 surface receptors.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
James CO, Huang MB, Khan M, Garcia-Barrio M, Powell MD, Bond VC 
In:
J Virol (2004) 78(6): 3099-109 
Differential regulation of responsiveness to fMLP and C5a upon dendritic cell maturation: correlation with receptor expression.
Categories:
Primary Cells and Media, Classical Media and Reagents 
Authors:
Yang D, Chen Q, Stoll S, Chen X, Howard OM, Oppenheim JJ 
In:
J Immunol (2000) 165(5): 2694-702 
Areca nut extracts mobilize calcium and release pro-inflammatory cytokines from various immune cells
Categories:
Primary Cells and Media 
Authors:
Faouzi M1, Neupane RP2, Yang J3, Williams P2, Penner R4,5 
In:
Scientific Reports () 8: 1075 
Adiponectin Reduces Bone Stiffness: Verified in a Three-Dimensional Artificial Human Bone Model In Vitro.
Categories:
Primary Cells and Media 
Authors:
Haugen S1, He J2, Sundaresan A3, Stunes AK4,5, Aasarød KM4,5, Tiainen H1, Syversen U4,6, Skallerud B2, Reseland JE1 
In:
Other () 9: 236 
Development of a preclinical humanized mouse model to evaluate acute toxicity of an influenza vaccine.
Categories:
Primary Cells and Media 
Authors:
Sasaki E1, Momose H1, Hiradate Y1, Furuhata K1, Mizukami T1, Hamaguchi I1. 
In:
OncoTarget () 9: 25751-25763 
RUNX1 regulates site specificity of DNA demethylation by recruitment of DNA demethylation machineries in hematopoietic cells
Categories:
Primary Cells and Media 
Authors:
Suzuki T1,2,3, Shimizu Y1,2, Furuhata E1,2, Maeda S1,2, Kishima M1,2, Nishimura H1,2, Enomoto S1,3, Hayashizaki Y2,4, Suzuki H1,2 
In:
Other () 1: 1699-1711 
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