B cell, human

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One of the two major classes of lymphocytes, B lymphocytes are white blood cells of the immune system that are derived from the bone marrow and spleen.

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
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Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Lymphocyte Growth Medium-3
LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 EO-117-AA 1e6 30-39% 70% Plasmid (general) 0.4 µg 20 µl 384-well
P3 EO-117-AA 1e6 30-39% 70% Plasmid (general) 0.4 µg 20 µl 384-well
B cell, human U-015 1e6 25-47% 84-92% Plasmid (general) 5 µg 100 µl I/II/2b
P3 96-EO-117 1e6 22-34% 63-77% Plasmid (general) 0.4 µg 20 µl 96-well
P3

EO-117

 1e6 22-34% 63-77% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (29)

Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC 
In:
Nat Biomed Eng (2023) 5: 647-660 
Highly efficient CRISPR-Cas9-mediated geneknockout in primary human B cells forfunctional genetic studies of Epstein-Barrvirus infection
Categories:
Transfection 
Authors:
Akidil E, Albanese M, Buschle A, Ruhle A, Pich D, Keppler OT, Hammerschmidt W.  
In:
PLoS Pathog (2021) 17(4): e1009117 
HIV-specific humoral immune responses by CRISPR/Cas9-edited B cells.
Categories:
Transfection 
Authors:
Hartweger H, McGuire AT, Horning M, Taylor JJ, Dosenovic P, Yost D, Gazumyan A, Seaman MS, Stamatatos L, Jankovic M, Nussenzweig MC. 
In:
J Exp Med (2019) 216 (6): 1301-1310 
CRISPR-Mediated Editing of the B Cell Receptor in Primary Human B Cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Greiner V, Bou Puerto R, Liu S, Herbel C, Carmona EM, Goldberg MS. 
In:
iScience (2019) 12: 369-378 
RNAi Transfection Optimized in Primary Naïve B Cells for the Targeted Analysis of Human Plasma Cell Differentiation
Categories:
Transfection 
Authors:
Shih T, De S, Barnes BJ 
In:
Frontiers in Immunology (2019) 10: 1652 
Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Larman HB, Scott ER, Wogan M, Oliveira G, Torkamani A, Schultz PG 
In:
Nucleic Acids Res (2014) 42(4): 9146-57 
Alterations in cholesterol metabolism restrict HIV-1 trans infection in nonprogressors
Categories:
Transfection 
Authors:
Rappocciolo G, Jais M, Piazza P, Reinhart TA, Berendam SJ, Garcia-Exposito L, Gupta P, Rinaldo CR 
In:
mBio (2014) 5(3): 1-11 
Congenital B cell lymphocytosis explained by novel germline CARD11 mutations.
Categories:
Transfection 
Authors:
Snow AL, Xiao W, Stinson JR, Lu W, Chaigne-Delalande B, Zheng L, Pittaluga S, Matthews HF, Schmitz R, Jhavar S, Kuchen S, Kardava L, Wang W, Lamborn IT, Jing H, Raffeld M, Moir S, Fleisher TA, Staudt LM, Su HC, Lenardo MJ. 
In:
J Exp Med (2012) 209(12): 2247-2261 
Attenuation of HIV-associated human B cell exhaustion by siRNA downregulation of inhibitory receptors.
Categories:
Transfection 
Authors:
Kardava L, Moir S, Wang W, Ho J, Buckner CM, Posada JG, O'Shea MA, Roby G, Chen J, Sohn HW, Chun TW, Pierce SK, Fauci AS. 
In:
J Clin Invest (2011) 121(7): 2614-24 
Selection of the alternative exon 1 from the cd5 gene down-regulates membrane level of the protein in B lymphocytes
Categories:
Transfection 
Authors:
Garaud S, Le Dantec C, Berthou C, Lydyard PM, Youinou P, Renaudineau Y 
In:
J Immunol (2008) 181(3): 2010-8 
RNA-loaded CD40-activated B cells stimulate antigen-specific T-cell responses in dogs with spontaneous lymphoma
Categories:
Transfection 
Authors:
Mason NJ, Coughlin CM, Overley B, Cohen JN, Mitchell EL, Colligon TA, Clifford CA, Zurbriggen A, Sorenmo KU, Vonderheide RH 
In:
Gene Ther (2008) 15(13): 955-65 
Nuclear CD40 interacts with c-Rel and enhances proliferation in aggressive B cell Lymphoma
Categories:
Transfection 
Authors:
Zhou HJ, Pham LV, Tamayo AT, Lin-Lee YC, Fu L, Yoshimura LC, Ford RJ 
In:
Blood (2007) 110(6): 2121-7 
Efficient nucleofection of primary human B cells and B-CLL cells induces apoptosis, which depends on the microenvironment and on the structure of transfected nucleic acids
Categories:
Transfection 
Authors:
Seiffert M, Stilgenbauer S, Döhner H, Lichter P 
In:
Leukemia (2007) 21(9): 1977-83 
Decreased expression of Kruppel-like factors in memory B cells induces the rapid response typical of secondary antibody responses
Categories:
Transfection 
Authors:
Good KL, Tangye SG 
In:
Proc Natl Acad Sci USA (2007) 104(33): 13420-5 
TACI regulates IgA production by APRIL in collaboration with HSPG
Categories:
Transfection 
Authors:
Sakurai D, Hase H, Kanno Y, Kojima H, Okumura K, Kobata T 
In:
Blood (2007) 109(7): 2961-7 
c-Abl Expression in Chronic Lymphocytic Leukemia Cells: Clinical and Therapeutic Implications
Categories:
Transfection 
Authors:
Lin K, Glenn MA, Harris RJ, Duckworth AD, Dennett S, Cawley JC, Zuzel M, Slupsky JR 
In:
Cancer Res (2006) 66(15): 7801-9 
Nuclear localization in the biology of the CD40 receptor in normal and neoplastic human B lymphocytes
Categories:
Transfection 
Authors:
Lin-Lee YC, Pham LV, Tamayo AT, Fu L, Zhou HJ, Yoshimura LC, Decker GL, Ford RJ 
In:
J Biol Chem (2006) 281(27): 18878-87 
High Mda-7 expression promotes malignant cell survival and p38 MAP kinase activation in chronic lymphocytic leukemia
Categories:
Transfection 
Authors:
Sainz-Perez A, Gary-Gouy H, Portier A, Davi F, Merle-Beral H, Galanaud P, Dalloul A 
In:
Leukemia (2006) 20(3): 498-504 
Regulation of the germinal center gene program by interferon (IFN) regulatory factor 8/IFN consensus sequence-binding protein
Categories:
Transfection 
Authors:
Lee CH, Melchers M, Wang H, Torrey TA, Slota R, Qi CF, Kim JY, Lugar P, Kong HJ, Farrington L, van der Zouwen B, Zhou JX, Lougaris V, Lipsky PE, Grammer AC and Morse HC 3rd 
In:
J Exp Med (2006) 203(1): 63-72 
Constitutive NF-kappaB and NFAT activation in aggressive B-cell lymphomas synergistically activates the CD154 gene and maintains lymphoma cell survival
Categories:
Transfection 
Authors:
Pham LV, Tamayo AT, Yoshimura LC, Lin-Lee YC and Ford RJ 
In:
Blood (2005) 106(12): 3940-3947 
Haploinsufficiency, rather than the effect of an excessive production of soluble CD95 (CD95TM), is the basis for ALPS Ia in a family with duplicated 3' splice site AG in CD95 intron 5 on one allele
Categories:
Transfection 
Authors:
Roesler J, Izquierdo JM, Ryser M, Rosen-Wolff A, Gahr M, Valcarcel J, Lenardo MJ and Zheng L 
In:
Blood (2005) 106(5): 1652-1659 
Chronic lymphocytic leukemia cells induce changes in gene expression of CD4 and CD8 T cells
Categories:
Transfection 
Authors:
Gorgun G, Holderried TA, Zahrieh D, Neuberg D and Gribben JG 
In:
J Clin Invest (2005) 115(7): 1797-1805 
Terminal differentiation into plasma cells initiates the replicative cycle of Epstein-Barr virus in vivo
Categories:
Transfection 
Authors:
Laichalk LL and Thorley-Lawson DA 
In:
J Virol (2005) 79(2): 1296-1307 
E47, IRF-4 and PU.1 synergize to induce B cell-specific activation of the Class II Transactivator promoter III (CIITA-PIII)
Categories:
Transfection 
Authors:
Van der Stoep N, Quinten E, Rezende MM and Van den Elsen PJ 
In:
Blood (2004) 104(9): 2849-2857 
Jak3-independent trafficking of the common gamma chain receptor subunit: chaperone function of Jaks revisited
Categories:
Transfection 
Authors:
Hofmann SR, Lam AQ, Frank S, Zhou YJ, Ramos HL, Kanno Y, Agnello D, Youle RJ and O'Shea JJ 
In:
Mol Cell Biol (2004) 24(11): 5039-5049 
New non-viral method for gene transfer into primary cells
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
RNA-transfected CD40-activated B cells induce functional T-cell responses against viral and tumor antigen targets: implications for pediatric immunotherapy
Categories:
Transfection 
Authors:
Coughlin CM, Vance BA, Grupp SA and Vonderheide RH 
In:
Blood (2004) 103: 2046-2054 
NF-kappaB regulates the expression of human complement receptor 2 gene
Categories:
Transfection 
Authors:
Tolnay M, Vereshchagina LA and Tsokos GC 
In:
J Immunol (2002) 169: 6236-6243 
RGS13 regulates germinal center B lymphocytes responsiveness to CXC chemokine ligand (CXCL)12 and CXCL13
Categories:
Transfection 
Authors:
Shi GX, Harrison K, Wilson GL, Moratz C and Kehrl JH 
In:
J Immunol (2002) 169(5): 2507-2515 
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