B cell, human

One of the two major classes of lymphocytes, B lymphocytes are white blood cells of the immune system that are derived from the bone marrow and spleen.

Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

TheraPEAK® X-VIVO® Media is a high-performing media series providing nutritionally adequate and balanced environments for a variety of hematopoietic cells including: 

  • T Cells
  • CAR-T cells
  • Peripheral blood lymphocytes (PBL)
  • Tumor infiltrating lymphocytes (TIL)
  • Human monocytes and macrophages
  • Lymphokine-activated killer cells (LAK)
  • Hematopoietic stem cells (HSCs)
  • Dendritic cells (DCs) 

Our scalable media are widely cited in scientific publications and proven to reliably work in many cell therapy applications around the world, from research stages to clinical trials to FDA-approved cell therapies. TheraPEAK® Products are manufactured according to GMP standards and can be safely and confidently used in your clinical processes. Our manufacturing sites are FDA registered with an ISO 13485 certified quality management system. 


There are two main versions of this media.

TheraPEAK® X-VIVO® 10 Media are  optimized for slower growing, less mature cells, such as hematopoietic stem cells

TheraPEAK® X-VIVO® 15 Media are most appropriate for rapidly growing cells, specifically cells of the immune system

TheraPEAK® X-VIVO® 10 Media and 15 Media are also available with recombinant transferrin instead of native human transferrin

Please note: X-VIVO™ 20 is currently available as an RUO grade catalog item. 

Storage: 2-8C, protect from light.

Catalog Offerings:

TheraPEAK® X-VIVO® Medium with native transferrin

Cat. No. NA

Cat.CCat. No. EU

Product

Size

BP04-743Q                            

 

           BEBP04-743Q

TheraPEAK® X-VIVO® 10 Medium without gentamicin and phenol red, contains native transferrin

1 L bottle

BP04-744Q

 

BEBP04-744Q

TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin

1 L bottle

08-879H

08-879P1

 

BE08-879H

BE08-879P1

TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin

5 L bag

1 L bag

TheraPEAK™ X-VIVO™ Medium with recombinant transferrin

Cat. No. NA

Cat. No. EU

Product

Size

N/A

BEBP02-055Q

TheraPEAK® X-VIVO® 10 Medium with L-glutamine, without gentamicin or phenol red, contains recombinant transferrin

1 L bottle

BP02-054Q

BP02-054P1

BEBP02-054Q

BEBP02-054P1

TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains recombinant transferrin

1 L bottle

1 L bag

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 EO-117-AA 1e6 30-39% 70% Plasmid (general) 0.4 µg 20 µl 384-well
P3 EO-117-AA 1e6 30-39% 70% Plasmid (general) 0.4 µg 20 µl 384-well
B cell, human U-015 1e6 25-47% 84-92% Plasmid (general) 5 µg 100 µl I/II/2b
P3 96-EO-117 1e6 22-34% 63-77% Plasmid (general) 0.4 µg 20 µl 96-well
P3

EO-117

1e6 22-34% 63-77% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (29)

Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC 
In:
Nat Biomed Eng (2023) 5: 647-660 
Categories:
Transfection 
Authors:
Akidil E, Albanese M, Buschle A, Ruhle A, Pich D, Keppler OT, Hammerschmidt W.  
In:
PLoS Pathog (2021) 17(4): e1009117 
Categories:
Transfection 
Authors:
Hartweger H, McGuire AT, Horning M, Taylor JJ, Dosenovic P, Yost D, Gazumyan A, Seaman MS, Stamatatos L, Jankovic M, Nussenzweig MC. 
In:
J Exp Med (2019) 216 (6): 1301-1310 
Categories:
Primary Cells and Media, Transfection 
Authors:
Greiner V, Bou Puerto R, Liu S, Herbel C, Carmona EM, Goldberg MS. 
In:
iScience (2019) 12: 369-378 
Categories:
Transfection 
Authors:
Shih T, De S, Barnes BJ 
In:
Frontiers in Immunology (2019) 10: 1652 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Larman HB, Scott ER, Wogan M, Oliveira G, Torkamani A, Schultz PG 
In:
Nucleic Acids Res (2014) 42(4): 9146-57 
Categories:
Transfection 
Authors:
Rappocciolo G, Jais M, Piazza P, Reinhart TA, Berendam SJ, Garcia-Exposito L, Gupta P, Rinaldo CR 
In:
mBio (2014) 5(3): 1-11 
Categories:
Transfection 
Authors:
Snow AL, Xiao W, Stinson JR, Lu W, Chaigne-Delalande B, Zheng L, Pittaluga S, Matthews HF, Schmitz R, Jhavar S, Kuchen S, Kardava L, Wang W, Lamborn IT, Jing H, Raffeld M, Moir S, Fleisher TA, Staudt LM, Su HC, Lenardo MJ. 
In:
J Exp Med (2012) 209(12): 2247-2261 
Categories:
Transfection 
Authors:
Kardava L, Moir S, Wang W, Ho J, Buckner CM, Posada JG, O'Shea MA, Roby G, Chen J, Sohn HW, Chun TW, Pierce SK, Fauci AS. 
In:
J Clin Invest (2011) 121(7): 2614-24 
Categories:
Transfection 
Authors:
Garaud S, Le Dantec C, Berthou C, Lydyard PM, Youinou P, Renaudineau Y 
In:
J Immunol (2008) 181(3): 2010-8 
Categories:
Transfection 
Authors:
Mason NJ, Coughlin CM, Overley B, Cohen JN, Mitchell EL, Colligon TA, Clifford CA, Zurbriggen A, Sorenmo KU, Vonderheide RH 
In:
Gene Ther (2008) 15(13): 955-65 
Categories:
Transfection 
Authors:
Zhou HJ, Pham LV, Tamayo AT, Lin-Lee YC, Fu L, Yoshimura LC, Ford RJ 
In:
Blood (2007) 110(6): 2121-7 
Categories:
Transfection 
Authors:
Seiffert M, Stilgenbauer S, Döhner H, Lichter P 
In:
Leukemia (2007) 21(9): 1977-83 
Categories:
Transfection 
Authors:
Good KL, Tangye SG 
In:
Proc Natl Acad Sci USA (2007) 104(33): 13420-5 
Categories:
Transfection 
Authors:
Sakurai D, Hase H, Kanno Y, Kojima H, Okumura K, Kobata T 
In:
Blood (2007) 109(7): 2961-7 
Categories:
Transfection 
Authors:
Lin K, Glenn MA, Harris RJ, Duckworth AD, Dennett S, Cawley JC, Zuzel M, Slupsky JR 
In:
Cancer Res (2006) 66(15): 7801-9 
Categories:
Transfection 
Authors:
Lin-Lee YC, Pham LV, Tamayo AT, Fu L, Zhou HJ, Yoshimura LC, Decker GL, Ford RJ 
In:
J Biol Chem (2006) 281(27): 18878-87 
Categories:
Transfection 
Authors:
Sainz-Perez A, Gary-Gouy H, Portier A, Davi F, Merle-Beral H, Galanaud P, Dalloul A 
In:
Leukemia (2006) 20(3): 498-504 
Categories:
Transfection 
Authors:
Lee CH, Melchers M, Wang H, Torrey TA, Slota R, Qi CF, Kim JY, Lugar P, Kong HJ, Farrington L, van der Zouwen B, Zhou JX, Lougaris V, Lipsky PE, Grammer AC and Morse HC 3rd 
In:
J Exp Med (2006) 203(1): 63-72 
Categories:
Transfection 
Authors:
Pham LV, Tamayo AT, Yoshimura LC, Lin-Lee YC and Ford RJ 
In:
Blood (2005) 106(12): 3940-3947 
Categories:
Transfection 
Authors:
Roesler J, Izquierdo JM, Ryser M, Rosen-Wolff A, Gahr M, Valcarcel J, Lenardo MJ and Zheng L 
In:
Blood (2005) 106(5): 1652-1659 
Categories:
Transfection 
Authors:
Gorgun G, Holderried TA, Zahrieh D, Neuberg D and Gribben JG 
In:
J Clin Invest (2005) 115(7): 1797-1805 
Categories:
Transfection 
Authors:
Laichalk LL and Thorley-Lawson DA 
In:
J Virol (2005) 79(2): 1296-1307 
Categories:
Transfection 
Authors:
Van der Stoep N, Quinten E, Rezende MM and Van den Elsen PJ 
In:
Blood (2004) 104(9): 2849-2857 
Categories:
Transfection 
Authors:
Hofmann SR, Lam AQ, Frank S, Zhou YJ, Ramos HL, Kanno Y, Agnello D, Youle RJ and O'Shea JJ 
In:
Mol Cell Biol (2004) 24(11): 5039-5049 
Categories:
Transfection 
Authors:
Gresch O, Engel FB, Nesic D, Tran TT, England HM, Hickman ES, Korner I, Gan L, Chen S, Castro-Obregon S, Hammermann R, Wolf J, Muller-Hartmann H, Nix M, Siebenkotten G, Kraus G and Lun K 
In:
Methods (2004) 33(2): 151-163 
Categories:
Transfection 
Authors:
Coughlin CM, Vance BA, Grupp SA and Vonderheide RH 
In:
Blood (2004) 103: 2046-2054 
Categories:
Transfection 
Authors:
Tolnay M, Vereshchagina LA and Tsokos GC 
In:
J Immunol (2002) 169: 6236-6243 
Categories:
Transfection 
Authors:
Shi GX, Harrison K, Wilson GL, Moratz C and Kehrl JH 
In:
J Immunol (2002) 169(5): 2507-2515 
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