Chronic lymphocytic leukemia cells induce changes in gene expression of CD4 and CD8 T cells

Authors:
Gorgun G, Holderried TA, Zahrieh D, Neuberg D and Gribben JG
In:
Source: J Clin Invest
Publication Date: (2005)
Issue: 115(7): 1797-1805
Research Area:
Cancer Research/Cell Biology
Cells used in publication:
B cell, human
Species: human
Tissue Origin: blood
B-CLL
Species: human
Tissue Origin: blood
Platform:
Nucleofectorâ„¢ I/II/2b
Abstract
To examine the impact of tumors on the immune system, we compared global gene expression profiles of peripheral blood T cells from previously untreated patients with B cell chronic lymphocytic leukemia (CLL) with those from age-matched healthy donors. Although the cells analyzed were not part of the malignant clone, analysis revealed differentially expressed genes, mainly involved in cell differentiation in CD4 cells and defects in cytoskeleton formation, vesicle trafficking, and cytotoxicity in CD8 cells of the CLL patients. In coculture experiments using CLL cells and T cells from healthy allogeneic donors, similar defects developed in both CD4 and CD8 cells. These changes were induced only with direct contact and were not cytokine mediated. Identification of the specific pathways perturbed in the T cells of cancer-bearing patients will allow us to assess steps to repair these defects, which will likely be required to enhance antitumor immunity.