Bone Marrow, Human, Unprocessed

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Unprocessed Human Bone Marrow Cells

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
bone marrow
Species:
human
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Research Area:
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Lymphocyte Growth Medium-3
LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations (34)

Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-ß Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation.
Categories:
Primary Cells and Media 
Authors:
Selvamurugan N1, He Z2, Rifkin D3, Dabovic B3, Partridge NC2 
In:
Stem Cells Intl (2017) : 2450327 
Stem Cell Delivery With Polymer Hydrogel for Treatment of Intervertebral Disc Degeneration: From 3D Culture to Design of the Delivery Device for Minimally Invasive Therapy.
Categories:
3D Cell Culture , Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kumar D, Lyness A, Gerges I, Lenardi C, Forsyth NR, Liu Y. 
In:
Cell Transplant (2016) 25(12): 2213-2220 
Cellular Reprogramming Allows Generation of Autologous Hematopoietic Progenitors From AML Patients That Are Devoid of Patient-Specific Genomic Aberrations.
Categories:
Primary Cells and Media 
Authors:
Salci KR, Lee JH, Laronde S, Dingwall S, Kushwah R, Fiebig-Comyn A, Leber B, Foley R, Dal Cin A, Bhatia M 
In:
Stem Cells (2015) 33(6): 1839-49 
Therapeutic potential of ixmyelocel-T, an expanded autologous multicellular therapy for treatment of ischemic cardiovascular diseases.
Categories:
 
Authors:
Ledford KJ, Murphy N, Zeigler F, Bartel RL, Tubo R 
In:
Stem Cell Res Ther (2015) 6(25): 1-14 
Progerin expression disrupts critical adult stem cell functions involved in tissue repair.
Categories:
Primary Cells and Media 
Authors:
Pacheco LM, Gomez LA, Dias J, Ziebarth NM, Howard GA, Schiller PC 
In:
Other (2014) 6(12): 1049-63 
Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.
Categories:
Primary Cells and Media 
Authors:
Keats EC, Dominguez JM, Grant MB, Khan ZA 
In:
Stem Cells (2014) 32(6): 1649-60 
Antagonism of SET using OP449 enhances the efficacy of tyrosine kinase inhibitors and overcomes drug resistance in myeloid leukemia.
Categories:
Primary Cells and Media 
Authors:
Agarwal A, MacKenzie RJ, Pippa R, Eide CA, Oddo J, Tyner JW, Sears R, Vitek MP, Odero MD, Christensen DJ, Druker BJ 
In:
Clin Cancer Res (2014) 20(8): 2092-103 
Distinct functional programming of human fetal and adult monocytes.
Categories:
Primary Cells and Media 
Authors:
Krow-Lucal ER, Kim CC, Burt TD, McCune JM 
In:
Blood (2014) 123(12): 1897-904 
Pharmacokinetics of natural and engineered secreted factors delivered by mesenchymal stromal cells.
Categories:
Primary Cells and Media 
Authors:
Elman JS, Murray RC, Wang F, Shen K, Gao S, Conway KE, Yarmush ML, Tannous BA, Weissleder R4, Parekkadan B 
In:
PLoS ONE (2014) 9(2): ePub 
Cytokine production by bone marrow mononuclear cells in inherited bone marrow failure syndromes
Categories:
Primary Cells and Media 
Authors:
Matsui K, Giri N, Alter BP, Pinto LA 
In:
Br J of Haematol (2013) 163(1): 81-92 
Adenosine regulates bone metabolism via A1, A2A, and A2B receptors in bone marrow cells from normal humans and patients with multiple myeloma.
Categories:
Primary Cells and Media 
Authors:
He W, Mazumder A, Wilder T, Cronstein BN 
In:
FASEB J (2013) 27(9): 3446-54 
Extending human hematopoietic stem cell survival in vitro with adipocytes.
Categories:
 
Authors:
Glettig DL, Kaplan DL. 
In:
BioResearch Open Access (2013) 2(3): 179-85 
Evidence of a Hematopoietic Origin of Human Bone Marrow Stromal Cells
Categories:
Primary Cells and Media 
Authors:
Milwid JM, Li M,USA LeeM J, Yarmush ML, Parekkadan B 
In:
Other (2013) 1(2): 1000113 
Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Pezeshkian B, Donnelly C, Tamburo K, Geddes T, Madlambayan GJ 
In:
PLoS ONE (2013) 8(4): e60823 
ON01210.Na (Ex-RADH) Mitigates Radiation Damage through Activation of the AKT Pathway
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Anthony D. Kang, Stephen C. Cosenza,Marie Bonagura,Manoj Manair,M. V. Ramana Reddy, E. Premkumar Reddy 
In:
PLoS ONE (2013) 3: e58355 
Mesenchymal stem cells promote alveolar epithelial cell wound repair in vitro through distinct migratory and paracrine mechanisms.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Akram KM, Samad S, Spiteri MA, Forsyth NR. 
In:
Respir Res (2013) 14: 9 
Mesenchymal stem cells promote alveolar epithelial cell wound repair in vitro through distinct migratory and paracrine mechanisms
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Akram KM, Samad S, Spiteri MA, Forsyth NR 
In:
Respir Res (2013) 14: 9 
Density gradient centrifugation compromises bone marrow mononuclear cell yield.
Categories:
Primary Cells and Media 
Authors:
Pösel C, Möller K, Fröhlich W, Schulz I, Boltze J, Wagner DC. 
In:
PLoS ONE (2012) 7(12): e50293 
Engineered endothelial progenitor cells that overexpress prostacyclin protect vascular cells.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Liu Q, Xi Y, Terry T, So SP, Mohite A, Zhang J, Wu G, Liu X, Cheng J, Ruan KH, Willerson JT, Dixon RA. 
In:
J Cell Physiol (2012) 22(7): 2907-16 
Unique responses of stem cell-derived vascular endothelial and mesenchymal cells to high levels of glucose
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Keats E, Khan ZA 
In:
PLoS ONE (2012) 7(6): e38752 
Bone progenitors produced by direct osteogenic differentiation of the unprocessed bone marrow demonstrate high osteogenic potential in vitro and in vivo
Categories:
Primary Cells and Media 
Authors:
Ginis I, Weinreb M, Abramov N, Shinar D, Merchav S, Schwartz A, Shirvan M. 
In:
BioResearch Open Access (2012) 1(2): 69-78 
Long-term effects of intravitreal injection of GMP-grade bone-marrow-derived CD34+ cells in NOD-SCID mice with acute ischemia-reperfusion injury.
Categories:
Primary Cells and Media 
Authors:
Park SS, Caballero S, Bauer G, Shibata B, Roth A, Fitzgerald PG, Forward KI, Zhou P, McGee J, Telander DG, Grant MB, Nolta JA. 
In:
Invest Ophthalmol Vis Sci (2012) 53(2): 986-94 
Bone marrow-derived CMPs and GMPs represent highly functional proangiogenic cells: implications for ischemic cardiovascular disease
Categories:
Primary Cells and Media 
Authors:
Wara AK, Croce K, Foo S, Sun X, Icli B, Tesmenitsky Y, Esen F, Rosenzweig A, Feinberg MW. 
In:
Blood (2011) 118(24): 6461-4 
Widespread deregulation of phosphorylation-based signaling pathways in multiple myeloma cells: opportunities for therapeutic intervention.
Categories:
Primary Cells and Media 
Authors:
Fuhler GM, Diks SH, Peppelenbosch MP, Kerr WG. 
In:
Mol Med (2011) 17(7-8): 790-8 
Use of human mesenchymal stem cells as alternative source of smooth muscle cells in vessel engineering
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gong Z, Niklason LE 
In:
Methods Mol Biol (2011) 698: 279-94 
The effect of transcatheter injections on cell viability and cytokine release of mononuclear cells.
Categories:
Primary Cells and Media 
Authors:
El Khoury R, Misra V, Sharma S, Cox CS, Walker P, Grotta JC, Gee A, Suzuki S, Savitz SI. 
In:
Other (2010) 31(8): 1488-92 
EF1alpha and RPL13a represent normalization genes suitable for RT-qPCR analysis of bone marrow derived mesenchymal stem cells
Categories:
Primary Cells and Media 
Authors:
Curtis KM, Gomez LA, Rios C, Garbayo E, Raval AP, Perez-Pinzon MA, Schiller PC 
In:
BMC Mol Biol (2010) 11: 61 
A novel serum-free medium for the expansion of human mesenchymal stem cells.
Categories:
Primary Cells and Media 
Authors:
Chase LG, Lakshmipathy U, Solchaga LA, Rao MS, Vemuri MC. 
In:
Stem Cell Res Ther (2010) 1(1): 8 
Activation of signal transducers and activators of transcription 3 and focal adhesion kinase by stromal cell-derived factor 1 is required for migration of human mesenchymal stem cells in response to tumor cell-conditioned medium
Categories:
Primary Cells and Media 
Authors:
Gao H, Priebe W, Glod J, Banerjee D 
In:
Stem Cells (2009) 27(4): 857-65 
Blood outgrowth endothelial cells from cord blood and peripheral blood: angiogenesis-related characteristics in vitro.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
van Beem RT, Verloop RE, Kleijer M, Noort WA, Loof N, Koolwijk P, van der Schoot CE, van Hinsbergh VW, Zwaginga JJ. 
In:
J Thromb Haemost (2009) 7(1): 217-226 
Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs).
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gong Z, Niklason LE. 
In:
FASEB J (2008) 22(6): 1635-48 
Circulating progenitor cells can be reliably identified on the basis of aldehyde dehydrogenase activity.
Categories:
Primary Cells and Media 
Authors:
Povsic TJ, Zavodni KL, Kelly FL, Zhu S, Goldschmidt-Clermont PJ, Dong C, Peterson ED. 
In:
J Am Coll Cardiol (2007) 50(23): 2243-8 
Regulation of survivin expression through Bcr-Abl/MAPK cascade: targeting survivin overcomes imatinib resistance and increases imatinib sensitivity in imatinib-responsive CML cells
Categories:
Transfection 
Authors:
Carter BZ, Mak D, Schober WD, Cabreira-Hansen M, Beran M, McQueen T, Chen W, and Andreeff M 
In:
Blood (2006) 107(4): 1555-1563 
Impact of starting material (fresh versus cryopreserved marrow) on mesenchymal stem cell culture
Categories:
Primary Cells and Media 
Authors:
Kaplan A1,2, Sackett K3, Sumstad D4, Kadidlo D4, McKenna DH3,4 
In:
Transfusion () 9: 2216-2219 
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