Bone Marrow, Human, Unprocessed

Unprocessed Human Bone Marrow Cells

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
bone marrow
Species:
human
Research Area:
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Suspension

Recommended Media

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and human or recombinant transferrin.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations (34)

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Primary Cells and Media 
Authors:
Selvamurugan N1, He Z2, Rifkin D3, Dabovic B3, Partridge NC2 
In:
Stem Cells Intl (2017) : 2450327 
Categories:
3D Cell Culture , Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kumar D, Lyness A, Gerges I, Lenardi C, Forsyth NR, Liu Y. 
In:
Cell Transplant (2016) 25(12): 2213-2220 
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Primary Cells and Media 
Authors:
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In:
Stem Cells (2015) 33(6): 1839-49 
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Authors:
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In:
Stem Cell Res Ther (2015) 6(25): 1-14 
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Primary Cells and Media 
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Pacheco LM, Gomez LA, Dias J, Ziebarth NM, Howard GA, Schiller PC 
In:
Other (2014) 6(12): 1049-63 
Categories:
Primary Cells and Media 
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Keats EC, Dominguez JM, Grant MB, Khan ZA 
In:
Stem Cells (2014) 32(6): 1649-60 
Categories:
Primary Cells and Media 
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Clin Cancer Res (2014) 20(8): 2092-103 
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Primary Cells and Media 
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Blood (2014) 123(12): 1897-904 
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Primary Cells and Media 
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PLoS ONE (2014) 9(2): ePub 
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Br J of Haematol (2013) 163(1): 81-92 
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Primary Cells and Media 
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FASEB J (2013) 27(9): 3446-54 
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Authors:
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BioResearch Open Access (2013) 2(3): 179-85 
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Primary Cells and Media 
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Other (2013) 1(2): 1000113 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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In:
PLoS ONE (2013) 8(4): e60823 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Anthony D. Kang, Stephen C. Cosenza,Marie Bonagura,Manoj Manair,M. V. Ramana Reddy, E. Premkumar Reddy 
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PLoS ONE (2013) 3: e58355 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Akram KM, Samad S, Spiteri MA, Forsyth NR. 
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Respir Res (2013) 14: 9 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Respir Res (2013) 14: 9 
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Primary Cells and Media 
Authors:
Pösel C, Möller K, Fröhlich W, Schulz I, Boltze J, Wagner DC. 
In:
PLoS ONE (2012) 7(12): e50293 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
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Liu Q, Xi Y, Terry T, So SP, Mohite A, Zhang J, Wu G, Liu X, Cheng J, Ruan KH, Willerson JT, Dixon RA. 
In:
J Cell Physiol (2012) 22(7): 2907-16 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Keats E, Khan ZA 
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PLoS ONE (2012) 7(6): e38752 
Categories:
Primary Cells and Media 
Authors:
Ginis I, Weinreb M, Abramov N, Shinar D, Merchav S, Schwartz A, Shirvan M. 
In:
BioResearch Open Access (2012) 1(2): 69-78 
Categories:
Primary Cells and Media 
Authors:
Park SS, Caballero S, Bauer G, Shibata B, Roth A, Fitzgerald PG, Forward KI, Zhou P, McGee J, Telander DG, Grant MB, Nolta JA. 
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Invest Ophthalmol Vis Sci (2012) 53(2): 986-94 
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Primary Cells and Media 
Authors:
Wara AK, Croce K, Foo S, Sun X, Icli B, Tesmenitsky Y, Esen F, Rosenzweig A, Feinberg MW. 
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Blood (2011) 118(24): 6461-4 
Categories:
Primary Cells and Media 
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Mol Med (2011) 17(7-8): 790-8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Gong Z, Niklason LE 
In:
Methods Mol Biol (2011) 698: 279-94 
Categories:
Primary Cells and Media 
Authors:
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In:
Other (2010) 31(8): 1488-92 
Categories:
Primary Cells and Media 
Authors:
Curtis KM, Gomez LA, Rios C, Garbayo E, Raval AP, Perez-Pinzon MA, Schiller PC 
In:
BMC Mol Biol (2010) 11: 61 
Categories:
Primary Cells and Media 
Authors:
Chase LG, Lakshmipathy U, Solchaga LA, Rao MS, Vemuri MC. 
In:
Stem Cell Res Ther (2010) 1(1): 8 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
van Beem RT, Verloop RE, Kleijer M, Noort WA, Loof N, Koolwijk P, van der Schoot CE, van Hinsbergh VW, Zwaginga JJ. 
In:
J Thromb Haemost (2009) 7(1): 217-226 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gong Z, Niklason LE. 
In:
FASEB J (2008) 22(6): 1635-48 
Categories:
Primary Cells and Media 
Authors:
Povsic TJ, Zavodni KL, Kelly FL, Zhu S, Goldschmidt-Clermont PJ, Dong C, Peterson ED. 
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J Am Coll Cardiol (2007) 50(23): 2243-8 
Categories:
Transfection 
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In:
Blood (2006) 107(4): 1555-1563 
Categories:
Primary Cells and Media 
Authors:
Kaplan A1,2, Sackett K3, Sumstad D4, Kadidlo D4, McKenna DH3,4 
In:
Transfusion () 9: 2216-2219 
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