citations

Evidence of a Hematopoietic Origin of Human Bone Marrow Stromal Cells

Authors:

Milwid JM, Li M,USA LeeM J, Yarmush ML, Parekkadan B

In:

Source: Other
Publication Date: (2013)
Issue: 1(2): 1000113

Research Area:

Basic Research

Cells used in publication:

CD34+ cell, human: Species: human; Tissue Origin: blood
Mesenchymal stem cell (MSC), human: Species: human; Tissue Origin: bone marrow
Bone Marrow, Human, Unprocessed: Species: human; Tissue Origin: bone marrow
Mononuclear, bone marrow, human: Species: human; Tissue Origin: bone marrow

Culture Media:

MEM Alpha Eagle

Experiment

The author appears to have obtained unprocessed bone marrow and bone marrow CD34+ cells from Lonza. Using the fresh bone marrow, the customer isolated mononuclear cells via density gradient centrifugation and then either directly plated the mononuclear cells or selected for CD45+ or CD34+ cells using immunomagnetic separation. The cells were plated in a hMSC medium. The CD34+ surface marker is typically associated with hematapoietic stem cells (HSC), however, this paper seems to imply that a limited number of CD34+ cells plated in mesenchyaml stem cell (MSC) media will adhere and demonstrate mesenchymal stem cell like properties implying a connection between HSCs and MSCs.

Abstract

Bone marrow stromal cells (BMSCs) have been considered to be of an ontological lineage distinct from hematopoietic cells in the bone marrow. Numerous studies have shown that BMSCs in culture exhibit an immunophenotype negative for hematopoietic markers such as CD45, CD34 and CD11b. In this brief report, we discover that human BMSCs can be isolated by positive selection from CD45+ or CD34+ cells of whole bone marrow aspirates, but not CD11b+ fractions. Adherent cells from the CD34+ and CD45+ fractions displayed growth, morphology, surface markers, and in vitro multipotency consistent with BMSCs from whole marrow. These cells were then transplanted subcutaneously in mice and were found to support the formation of hematopoietic tissue in ectopic sites. These results suggest an unidentified link between human hematopoietic cells and BMSCs and may point to the existence of a common progenitor.