Mesenchymal stem cell (MSC), human

Bone marrow derived stem cells with the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. Generally considered a subpopulation of bone marrow stromal cells, although often the terms are used interchangeably.

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
bone marrow
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Adherent

Recommended Media

MSCGM was developed for growing large numbers of mesenchymal stem cells without inducing diferentiation.

Medium for differentiating human MSCs into chondrocytes.

TGF-beta 3 (PT-4124) is required as a growth factor for this medium.

Media for differentiating MSC into osteoblasts

Media for the differentiation of hMSC cells into adipocytes.

This kit consists of two media:
hMSC Adipogenic Induction Medium (consisting of  basal medium (PT-3102B) and Singlequots (PT-4135)
hMSC Adipogenic Maintenance Medium (consisting of basal medium (PT-3102A) and SingleQuots (PT-4122)

TheraPEAK™ MSCGM™ Mesenchymal Stem Cell Growth Medium, Serum-free (with L-glutamine, without phenol red and antibiotics).

TheraPEAK™MSCGM™ is a serum free medium for the growth of human Mesenchymal Stem Cells. TheraPEAK™ MSCGM™ is optimized for the multiple passage expansion of all types of hMSCs while still allowing for differentiation into the several desired lineages. Cells can be directly transitioned from serum-containing medium to TheraPEAK™ MSCGM™ with little to no adaptation time. For peak performance, it is recommended to use TheraPEAK™ MSCGM™ with Fibronectin coated plates

ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) is universally suitable for cryopreserving many cell types in the absence of fetal bovine serum (FBS), which is particularly advantageous for freezing of cells cultured in a serum-free and animal component-free environment.
This protein-free freezing medium contains no animal derived components, insulin, or hydrolysate, and maintains high cell viability upon recovery from frozen storage.

ProFreeze™ Medium requires the addition of 15% reagent or spectrophotometric grade dimethylsulfoxide (DMSO) at time of use.

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P1 96-FF-104 5e4 73-83% 53-81% Plasmid (general) 0.4 µg 20 µl 96-well
P1

FF-104

5e4 72-80% 56-75% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
MSC, human U-023 5e5 35-59% 72-84% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (133)

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Primary Cells and Media, 3D Cell Culture  
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Cancer Res (2009) 69(23): 8932-40 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media 
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Cell Transplant (2009) 18(2): 297-304 
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Primary Cells and Media 
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Stem Cells (2009) 27(10): 2469-77 
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Primary Cells and Media 
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Nucleic Acids Res (2009) 37(15): 4987-5000 
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Int J Oncol. (2009) 34(3): 619-27 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Van Campenhout A, Moran CS, Parr A, Clancy P, Rush C, Jakubowski H, Golledge J 
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Other (2009) 202(2): 557-566 
Categories:
Primary Cells and Media 
Authors:
Akter R, Rivas D, Geneau G, Drissi H, Duque G. 
In:
J Bone Joint Surg Am (2009) 24(2): 283-93 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
WM Jackson1, AB Aragon1,2, F Djouad1, Y Song1, SM Koehler1, LJ Nesti1,2, and RS Tuan1 
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J Tissue Eng. (2009) 3(2): 139-138 
Categories:
Primary Cells and Media 
Authors:
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In:
Proc Natl Acad Sci USA (2008) 105(52): 20764-9 
Categories:
Transfection 
Authors:
Maucksch C, Aneja MK, Hennen E, Bohla A, Hoffmann F, Elfinger M, Rosenecker J, Rudolph C 
In:
Nucleic Acids Res (2008) epub: online 
Categories:
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Authors:
Cironi L, Riggi N, Provero P, Wolf N, SuvÜ  ML, SuvÜ  D, Kindler V, Stamenkovic I 
In:
PLoS ONE (2008) 3(7): e2634 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Gong Z, Niklason LE. 
In:
FASEB J (2008) 22(6): 1635-48 
Categories:
Transfection 
Authors:
Sheyn D, Pelled G, Zilberman Y, Talasazan F, Frank JM, Gazit D, Gazit Z 
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Stem Cells (2008) 26(4): 1056-64 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sueblinvong V, Loi R, Eisenhauer PL, Bernstein IM, Suratt BT, Spees JL, Weiss DJ. 
In:
Am J Respir Crit Care Med (2008) 177(7): 701-11 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Jamieson WL, Shimizu S, D\'Ambrosio JA, Meucci O, Fatatis A 
In:
Cancer Res (2008) 68(6): 1715-22 
Categories:
Transfection 
Authors:
Yang DC, Tsay HJ, Lin SY, Chiou SH, Li MJ, Chang TJ, Hung SC 
In:
PLoS ONE (2008) 3(2): e1540 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Murdoch AD, Grady LM, Ablett MP, Katopodi T, Meadows RS, Hardingham TE 
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Stem Cells (2007) 25(11): 2786-96 
Categories:
Primary Cells and Media 
Authors:
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FASEB J (2007) 21(11): 2949-60 
Categories:
Transfection 
Authors:
Plotnikov AN, Shlapakova I, Szabolcs MJ, Danilo P, Lorell BH, Potapova IA, Lu Z, Rosen AB, Mathias RT, Brink PR, Robinson RB, Cohen IS, Rosen MR 
In:
Circulation (2007) 116(7): 706-13 
Categories:
Transfection 
Authors:
Ball SG, Shuttleworth CA, Kielty CM 
In:
J Cell Biol (2007) 177(3): 489-500 
Categories:
Transfection 
Authors:
Standal T, Abildgaard N, Fagerli UM, Stordal B, Hjertner O, Borset M, Sundan A 
In:
Blood (2007) 109(7): 3024-30 
Categories:
Transfection 
Authors:
Shih YR, Chen CN, Tsai SW, Wang YJ, Lee OK 
In:
Stem Cells (2006) 24(11): 2391-7 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang M, Crisostomo PR, Herring C, Meldrum KK, Meldrum DR. 
In:
Am J Physiol Regul Integr Comp Physiol (2006) 291(4): R880-4 
Categories:
Transfection 
Authors:
Aslan H, Zilberman Y, Arbeli V, Sheyn D, Matan Y, Liebergall M, Li JZ, Helm GA, Gazit D, Gazit Z 
In:
Tissue Eng (2006) 12(4): 877-89 
Categories:
Primary Cells and Media 
Authors:
Yokoo T, Fukui A, Ohashi T, Miyazaki Y, Utsunomiya Y, Kawamura T, Hosoya T, Okabe M, Kobayashi E 
In:
J Am Soc Nephrol (2006) 17(4): 1026-34 
Categories:
Primary Cells and Media 
Authors:
Tondreau T, Meuleman N, Delforge A, Dejeneffe M, Leroy R, Massy M, Mortier C, Bron D, Lagneaux L. 
In:
Stem Cells (2005) 23(8): 1105-12 
Categories:
Transfection 
Authors:
Haleem-Smith H, Derfoul A, Okafor C, Tuli R, Olsen D, Hall DJ and Tuan RS 
In:
Mol Biotechnol (2005) 30(1): 9-20 
Categories:
Transfection 
Authors:
Rosen MR, Robinson RB, Brink P, Cohen IS 
In:
Anat Rec A (2004) 280(2): 1046-52 
Categories:
Primary Cells and Media 
Authors:
Kaplan A1,2, Sackett K3, Sumstad D4, Kadidlo D4, McKenna DH3,4 
In:
Transfusion () 9: 2216-2219 
Categories:
Primary Cells and Media 
Authors:
Altmayer NC#1, Galata V#2, Warschburger N1, Keller A2, Meese E#1, Fischer U#1 
In:
OncoTarget () 2: 1803-1812 
Categories:
Primary Cells and Media 
Authors:
Amer MH1, Rose FRAJ1, Shakesheff KM1, White LJ2 
In:
Stem Cell Res Ther () 39:  
Categories:
Primary Cells and Media 
Authors:
Bagheri L1, Pellati A1, Rizzo P1, Aquila G2,3, Massari L1, De Mattei M1, Ongaro A1. 
In:
J Tissue Eng. () 2: 304-315 
Categories:
Primary Cells and Media 
Authors:
Blondiaux E1,2, Pidial L1, Autret G1,3, Rahmi G1, Balvay D1,3, Audureau E4, Wilhelm C5, Guerin CL1, Bruneval P6, Silvestre JS1, Menasché P1,7, Clément O1 
In:
J Tissue Eng. () 12: 3417-3427 
Categories:
Primary Cells and Media 
Authors:
Costa V1, Carina V1, Fontana S2, De Luca A1, Monteleone F2, Pagani S3, Sartori M4, Setti S5, Faldini C6, Alessandro R2, Fini M3,4, Giavaresi G1,3 
In:
J Cell Physiol () 233: 1558-1573 
Categories:
Primary Cells and Media 
Authors:
Dabrowska S1, Del Fattore A2, Karnas E3,4, Frontczak-Baniewicz M5, Kozlowska H6, Muraca M7, Janowski M1,8, Lukomska B1 
In:
Int J Nanomedicine () :  
Categories:
Primary Cells and Media 
Authors:
Deshmukh V1, Hu H2, Barroga C3, Bossard C4, Kc S5, Dellamary L6, Stewart J7, Chiu K8, Ibanez M9, Pedraza M10, Seo T11, Do L12, Cho S13, Cahiwat J14, Tam B15, Tambiah JRS16, Hood J17, Lane NE18, Yazici Y19. 
In:
Osteoarthritis Cartilage () 26: 18-27 
Categories:
Primary Cells and Media 
Authors:
Frith JE1,2, Kusuma GD3, Carthew J3, Li F3, Cloonan N4, Gomez GA5,6, Cooper-White JJ7,8,9. 
In:
Nat Commun. () 9: 257 
Categories:
Primary Cells and Media 
Authors:
Frith JE1,2, Kusuma GD3, Carthew J3, Li F3, Cloonan N4, Gomez GA5,6, Cooper-White JJ7,8,9 
In:
Nat Commun. () 9: 257 
Categories:
Primary Cells and Media 
Authors:
Hanga MP1,2, Murasiewicz H3,4, Pacek AW3, Nienow AW1,3,2, Coopman K1, Hewitt CJ1,2. 
In:
Other () 92: 1577-1589 
Categories:
Primary Cells and Media 
Authors:
Hariri A1, Lemaster J1, Wang J1, Jeevarathinam AS1, Chao DL2, Jokerst JV1,3,4 
In:
Other () 9: 10-20 
Categories:
Primary Cells and Media 
Authors:
Kang YG1, Jang H2, Yang TD1, Notbohm J3, Choi Y1, Park Y2, Kim BM1 
In:
J Bio Med Opt () 23: 1-8 
Categories:
Primary Cells and Media 
Authors:
Kruk JS1, Bermeo S1,2, Skarratt KK1, Fuller SJ1, Duque G1,3,4. 
In:
Other () 25: 43-51 
Categories:
Primary Cells and Media 
Authors:
Liu Y1,2,3,4, Zhang X3,4, Chen J2, Li T2 
In:
Med. Sci. () 24: 264-271 
Categories:
Primary Cells and Media 
Authors:
Simon M1, Major B2, Vácz G1, Kuten O3,4, Hornyák I1,3, Hinsenkamp A1, Kardos D1, Bagó M1, Cseh D5, Sárközi A5, Horvathy D1, Nehrer S4, Lacza Z1,3 
In:
Stem Cells Intl () 2018: 4854619 
Categories:
Primary Cells and Media 
Authors:
Sinclair A1, O'Kelly MB1, Bai T1, Hung HC1, Jain P1, Jiang S1 
In:
Other () 30: e1803087 
Categories:
Primary Cells and Media 
Authors:
Lee JW1, Hoshino A2, Inoue K3, Saitou T4,5, Uehara S6, Kobayashi Y7, Ueha S8, Matsushima K8, Yamaguchi A9, Imai Y3,10,11, Iimura T12,13,14 
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Nat Commun. () 8: 2226 
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