Potentiation of osteoclastogenesis by adipogenic conversion of bone marrow-derived mesenchymal stem cells.

Mori K, Suzuki K, Hozumi A, Goto H, Tomita M, Koseki H, Yamashita S, Osaki M
Source: Biomed Res Int.
Publication Date: (2014)
Issue: 35(2): 153-9
Research Area:
Stem Cells
Basic Research
Cells used in publication:
Mesenchymal stem cell (MSC), human
Species: human
Tissue Origin: bone marrow
Osteoclast precursor (OCP), human
Species: human
Tissue Origin: bone marrow
Bone marrow-derived mesenchymal stem cells (BMSCs) are the indispensable component of the bone marrow, being the common precursors for adipocytes and osteoblasts. We show here that adipogenic differentiation resulted in increase in the production of adipocyte markers, such as adiponectin,fatty-acid binding proteins (FABP4), peroxisome proliferator-activated receptor ? (PPAR?), as well as the receptor activator of nuclear-?B ligand (RANKL). Co-culture of osteoclast precursors (OCPs) with BMSCs-derived adipocytes significantly enhanced osteoclast differentiation with low-dose RANKL, whose levels alone could not promote osteoclastogenesis. These results demonstrate for the first time that adipogenic differentiation of BMSCs plays a pivotal role in maintaining bone homeostasis.