T cell, human stim.

Periphal blood lymphocytes; pre-stimulated.

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and human or recombinant transferrin.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T cell, human T-023 5e6 36-58% Plasmid (general) 1 µg 100 µl I/II/2b
P3

EO-115

1e6 63-77% 47-72% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 96-EO-115 1e6 63.3-77.3% 47.3-71.3% Plasmid (general) 0.4 µg 20 µl Shuttle

Citations (121)

Categories:
Primary Cells and Media, Transfection 
Authors:
Schober K, Müller TR, Gökmen F, Grassmann S, Effenberger M, Poltorak M, Stemberger C, Schumann K, Roth TL, Marson A, Busch DH. 
In:
Nat Biomed Eng (2019) 10: 01 
Categories:
Primary Cells and Media, Transfection 
Authors:
Oh SA, Seki A, Rutz S.  
In:
Curr Protoc Immunol (2019) 124(1): e69 
Categories:
Transfection 
Authors:
Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F. 
In:
Nature (2019) 10(1): 212 
Categories:
Primary Cells and Media, Transfection 
Authors:
Hultquist JF, Hiatt J, Schumann K, McGregor MJ, Roth TL, Haas P, Doudna JA, Marson A, Krogan NJ.  
In:
Nat Protocols (2019) 14(1): 1-27 
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang 
In:
J Med Genetics (2019) 56: 10–17 
Categories:
Transfection 
Authors:
Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME,  Ashworth A, Marson A.  
In:
Cell (2018) 175(7): 1985-1971 
Categories:
Transfection 
Authors:
Ting PY, Parker AE, Lee JS, Trussell C, Sharif O, Luna F, Federe G, Barnes SW, Walker JR, Vance J, Gao MY, Klock HE, Clarkson S, Russ C, Miraglia LJ, Cooke MP, Boitano AE, McNamara P, Lamb J, Schmedt C, Snead JL. 
In:
Nat Methods (2018) 15(11):  
Categories:
Serum-free and Speciality Media 
Authors:
Castiglia S, Adamini A, Rustichelli D, Castello L, Mareschi K, Pinnetta G, Leone M, Mandese A, Ferrero I, Mesiano G, Fagioli F.  
In:
J Transl Med (2018) 16: 237 
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Vakulskas CA, Dever DP, Rettig GR, Turk R, Jacobi AM, Collingwood MA, Bode NM, McNeill MS, Yan S, Camarena J, Lee CM, Park SH, Wiebking V, Bak RO, Gomez-Ospina N, Pavel-Dinu M, Sun W6, Bao G, Porteus MH, Behlke MA 
In:
Nat Med (2018) 24(8): 1216-1224 
Categories:
Primary Cells and Media, Transfection, Laboratory Instrumentation  
Authors:
Roth TL, Puig-Saus C, Yu R, Shifrut E, Carnevale J, Li PJ, Hiatt J, Saco J, Krystofinski P, Li H, Tobin V, Nguyen DN, Lee MR, Putnam AL, Ferris AL, Chen JW, Schickel JN, Pellerin L, Carmody D, Alkorta-Aranburu G, Del Gaudio D, Matsumoto H, Morell M, Mao Y, Cho M, Quadros RM, Gurumurthy CB, Smith B, Haugwitz M, Hughes SH, Weissman JS, Schumann K, Esensten JH, May AP, Ashworth A, Kupfer GM, Greeley SAW, Bacchetta R, Meffre E, Roncarolo MG, Romberg N, Herold KC, Ribas A, Leonetti MD, Marson A. 
In:
Nature (2018) 559: 405-9 
Categories:
Transfection 
Authors:
Hu B, Zou Y, Zhang L, Tang J, Niedermann G, Firat E, Huang X, Zhu X.  
In:
Hum Gene Ther (2018) :  
Categories:
Primary Cells and Media, Transfection 
Authors:
Seki A, Rutz S. 
In:
J Exp Med (2018) 215(3): 985-997 
Categories:
Serum-free and Speciality Media 
Authors:
Medvec AR, Ecker C, Kong H, Winters EA, Glover J, Varela-Rohena A, Riley JL.  
In:
Mol Ther Methods Clin Dev. (2017) 7; 8: 65-74 
Categories:
Transfection 
Authors:
Hudecek M1, Izsvák Z2, Johnen S3, Renner M4, Thumann G5, Ivics Z4. 
In:
Clin Exp Immunol (2017) 52(4): 355(80) 
Categories:
Transfection 
Authors:
Bak RO, Porteus MH. 
In:
Cell Rep (2017) 20(3): 750-756 
Categories:
Transfection 
Authors:
Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H. 
In:
Frontiers in Immunology (2017) 1: 1-9 
Categories:
Transfection 
Authors:
Rupp LJ1, Schumann K, Roybal KT, Gate RE, Ye CJ, Lim WA, Marson A. 
In:
Scientific Reports (2017) 7 (1): 737 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, Piechocka-Trocha , Krogan NJ, Marson A, Sabatini DM, Lander ES, Hacohen N, Walker BD. 
In:
Nat Genet (2017) 49(2): 193-203 
Categories:
Transfection 
Authors:
Hultquist JF, Schumann K, Woo JM, Manganaro L, McGregor MJ, Doudna J, Simon V, Krogan NJ, Marson A. 
In:
Cell Rep (2016) 17(5): 1438-1452 
Categories:
Transfection 
Authors:
Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. 
In:
Cell (2016) 167(1): 219-232 
Categories:
Transfection 
Authors:
Thomas C. Whisenant, Eigen R. Peralta, Lauren D. Aarreberg, Nina J. Gao, Steven R. Head, Phillip Ordoukhanian, Jamie R. Williamson, Daniel R. Salomon 
In:
PLoS ONE (2015) 10(12): e0144409 
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn, Matthew H Porteus 
In:
Nat Biotechnol (2015) 10.1038: nbt.3290 
Categories:
Transfection 
Authors:
Anne-Christine Field, Conrad Vink, Richard Gabriel, Roua Al-Subki, Manfred Schmidt, Nicholas Goulden, Hans Stauss, Adrian Thrasher, Emma Morris, Waseem Qasim 
In:
PLoS ONE (2013) 8(6): e68201 
Categories:
 
Authors:
Dobritsa SV, Kuok IT, Nguyen H, Webster JC, Spragg AM, Morley T, Carr GJ. 
In:
J Biomol Screen (2013) 18(1): 75-84 
Categories:
Transfection 
Authors:
Torikai H, Reik A, Liu PQ, Zhou Y, Zhang L, Maiti S, Huls H, Miller JC, Kebriaei P, Rabinovitch B, Lee DA, Champlin RE, Bonini C, Naldini L, Rebar EJ, Gregory PD, Holmes MC, Cooper LJ. 
In:
Blood (2012) 119(24): 5697-5705 
Categories:
Serum-free and Speciality Media 
Authors:
Renier J. Brentjens, Isabelle Rivière, Jae H. Park, Marco L. Davila, Xiuyan Wang, Jolanta Stefanski, Clare Taylor, Raymond Yeh, Shirley Bartido, Oriana Borquez-Ojeda, Malgorzata Olszewska, Yvette Bernal, Hollie Pegram, Mark Przybylowski, Daniel Hollyman, Yelena Usachenko, Domenick Pirraglia, James Hosey, Elmer Santos, Elizabeth Halton, Peter Maslak, David Scheinberg, Joseph Jurcic, Mark Heaney, Glenn Heller, Mark Frattini, Michel Sadelain 
In:
Blood (2011) 118 (18): 4817-4828 
Categories:
 
Authors:
Lisboa FA, Warren J, Sulkowski G, Aparicio M, David G, Zudaire E, Dveksler GS 
In:
J Biol Chem (2011) 286(9): 7577-86 
Categories:
Transfection 
Authors:
Nakazawa Y, Huye LE, Dotti G, Foster AE, Vera JF, Manuri PR, June CH, Rooney CM, Wilson MH. 
In:
J Immunother (2009) 32(8): 826-36 
Categories:
 
Authors:
Levy DS, Kahana JA, Kumar R. 
In:
Blood (2009) 113(8): 1723-9 
Categories:
Transfection 
Authors:
Kirchhof MG, Chau LA, Lemke CD, Vardhana S, Darlington PJ, MÜ¡rquez ME, Taylor R, Rizkalla K, Blanca I, Dustin ML, Madrenas J 
In:
J Immunol (2008) 181(3): 1927-36 
Categories:
Transfection 
Authors:
Fos C, Salles A, Lang V, Carrette F, Audebert S, Pastor S, Ghiotto M, Olive D, Bismuth G, Nunes JA 
In:
J Immunol (2008) 181(3): 1969-77 
Categories:
Transfection 
Authors:
Oliere S, Arguello M, Mesplede T, Tumilasci V, Nakhaei P, Stojdl D, Sonenberg N, Bell J, Hiscott J 
In:
J Virol (2008) 82(12): 5735-49 
Categories:
Transfection 
Authors:
Doi Y, Oki S, Ozawa T, Hohjoh H, Miyake S, Yamamura T 
In:
Proc Natl Acad Sci USA (2008) 105(24): 8381-6 
Categories:
Transfection 
Authors:
Garaude J, Farras R, Bossis G, Charni S, Piechaczyk M, Hipskind RA, Villalba M 
In:
J Immunol (2008) 180(9): 5983-90 
Categories:
Transfection 
Authors:
Huang X, Guo H, Kang J, Choi S, Zhou TC, Tammana S, Lees CJ, Li ZZ, Milone M, Levine BL, Tolar J, June CH, Scott McIvor R, Wagner JE, Blazar BR, Zhou X 
In:
Mol Ther (2008) 16(3): 580-9 
Categories:
Transfection 
Authors:
van Grevenynghe J, Procopio FA, He Z, Chomont N, Riou C, Zhang Y, Gimmig S, Boucher G, Wilkinson P, Shi Y, Yassine-Diab B, Said EA, Trautmann L, El Far M, Balderas RS, Boulassel MR, Routy JP, Haddad EK, Sekaly RP 
In:
Nat Med (2008) 14(3): 266-74 
Categories:
Transfection 
Authors:
Lioudyno MI, Kozak JA, Penna A, Safrina O, Zhang SL, Sen D, Roos J, Stauderman KA, Cahalan MD 
In:
Proc Natl Acad Sci USA (2008) 105(6): 2011-6 
Categories:
Transfection 
Authors:
Kaminuma O, Kitamura F, Kitamura N, Hiroi T, Miyoshi H, Miyawaki A, Miyatake S 
In:
J Immunol (2008) 180(1): 319-26 
Categories:
Transfection 
Authors:
Real E, Faure S, Donnadieu E, Delon J 
In:
J Immunol (2007) 179(9): 5649-52 
Categories:
Transfection 
Authors:
Juang YT, Sumibcay L, Tolnay M, Wang Y, Kyttaris VC, Tsokos GC 
In:
J Immunol (2007) 179(7): 4884-9 
Categories:
Transfection 
Authors:
Ruppelt A, Mosenden R, Grönholm M, Aandahl EM, Tobin D, Carlson CR, Abrahamsen H, Herberg FW, Carpén O, Taskén K 
In:
J Immunol (2007) 179(8): 5159-68 
Categories:
Transfection 
Authors:
Gorelik G, Fang JY, Wu A, Sawalha AH, Richardson B 
In:
J Immunol (2007) 179(8): 5553-63 
Categories:
Transfection 
Authors:
Giri B, Dixit VD, Ghosh MC, Collins GD, Khan IU, Madara K, Weeraratna AT, Taub DD 
In:
Eur J Immunol (2007) 37(8): 2104-16 
Categories:
Transfection 
Authors:
Nie Z, Bren GD, Vlahakis SR, Schimnich AA, Brenchley JM, Trushin SA, Warren S, Schnepple DJ, Kovacs CM, Loutfy MR, Douek DC, Badley AD 
In:
J Virol (2007) 81(13): 6947-56 
Categories:
Transfection 
Authors:
Srivastava S, Banerjee H, Chaudhry A, Khare A, Sarin A, George A, Bal V, Durdik JM, Rath S 
In:
J Immunol (2007) 179(2): 797-803 
Categories:
Transfection 
Authors:
Bopp T, Becker C, Klein M, Klein-Heßling S, Palmetshofer A, Serfling E, Heib V, Becker M, Kubach J, Schmitt S, Stoll S, Schild H, Staege MS, Stassen M, Jonuleit H, Schmitt E 
In:
J Exp Med (2007) 204(6): 1303-1310 
Categories:
Transfection 
Authors:
Schulte M, Reiss K, Lettau M, Maretzky T, Ludwig A, Hartmann D, de Strooper B, Janssen O, Saftig P 
In:
Cell Death Differ (2007) 14(5): 1040-9 
Categories:
Transfection 
Authors:
Mummidi S, Adams LM, Vancompernolle SE, Kalkonde M, Camargo JF, Kulkarni H, Bellinger AS, Bonello G, Tagoh H, Ahuja SS, Unutmaz D, Ahuja SK 
In:
J Immunol (2007) 178(9): 5668-81 
Categories:
Transfection 
Authors:
Nakayamada S, Saito K, Nakano K, Tanaka Y 
In:
Arthritis Rheum (2007) 56(5): 1559-1568 
Categories:
Transfection 
Authors:
Oliveira JB, Bidère N, Niemela JE, Zheng L, Sakai K, Nix CP, Danner RL, Barb J, Munson PJ, Puck JM, Dale J, Straus SE, Fleisher TA, Lenardo MJ 
In:
Proc Natl Acad Sci USA (2007) 104(21): 8953-8958 
Categories:
Transfection 
Authors:
Nakata Y, Shetzline S, Sakashita C, Kalota A, Rallapalli R, Rudnick SI, Zhang Y, Emerson SG, Gewirtz AM 
In:
Mol Cell Biol (2007) 27(6): 2048-58 
Categories:
Transfection 
Authors:
Wu B, Crampton SP, Hughes CC 
In:
Immunity (2007) 26(2): 227-39 
Categories:
Transfection 
Authors:
Choy JC, Wang Y, Tellides G, Pober JS 
In:
Proc Natl Acad Sci USA (2007) 104(4): 1313-8 
Categories:
Transfection 
Authors:
Simonson WT, Franco SJ, Huttenlocher A 
In:
J Immunol (2006) 177(11): 7707-14 
Categories:
Transfection 
Authors:
Collins S, Wolfraim LA, Drake CG, Horton MR, Powell JD 
In:
J Immunol (2006) 177(12): 8301-5 
Categories:
Transfection 
Authors:
Campello S, Lacalle RA, Bettella M, Manes S, Scorrano L, Viola A 
In:
J Exp Med (2006) 203(13): 2879-86 
Categories:
Transfection 
Authors:
Srivastava S, Li Z, Ko K, Choudhury P, Albaqumi M, Johnson AK, Yan Y, Backer JM, Unutmaz D, Coetzee WA, Skolnik EY 
In:
Mol Cell (2006) 24(5): 665-75 
Categories:
Transfection 
Authors:
Avota E, Harms H, Schneider-Schaulies S 
In:
Cell Microbiol (2006) 8(11): 1826-39 
Categories:
Transfection 
Authors:
Venzke S, Michel N, Allespach I, Fackler OT, Keppler OT 
In:
J Virol (2006) 80(22): 11141-52 
Categories:
Transfection 
Authors:
Zimmerman ES, Sherman MP, Blackett JL, Neidleman JA, Kreis C, Mundt P, Williams SA, Warmerdam M, Kahn J, Hecht FM, Grant RM, de Noronha CM, Weyrich AS, Greene WC, Planelles V 
In:
J Virol (2006) 80(21): 10407-18 
Categories:
Transfection 
Authors:
Tavano R, Contento RL, Baranda SJ, Soligo M, Tuosto L, Manes S, Viola A 
In:
Nat Cell Biol (2006) 8(11): 1270-6 
Categories:
Transfection 
Authors:
Valk E, Leung R, Kang H, Kaneko K, Rudd CE, Schneider H 
In:
Immunity (2006) 25(5): 807-821 
Categories:
Transfection 
Authors:
Kunzmann S, Wright JR, Steinhilber W, Kramer BW, Blaser K, Speer CP, Schmidt-Weber C 
In:
Am J Physiol Lung Cell Mol Physiol (2006) 291(4): L747-56 
Categories:
Transfection 
Authors:
Lowenberg M, Verhaar AP, Bilderbeek J, Marle JV, Buttgereit F, Peppelenbosch MP, van Deventer SJ, Hommes DW 
In:
EMBO Rep (2006) 7(10): 1023-9 
Categories:
Transfection 
Authors:
Garcia-Bernal D, Sotillo-Mallo E, Nombela-Arrieta C, Samaniego R, Fukui Y, Stein JV, Teixido J 
In:
J Immunol (2006) 177(8): 5215-25 
Categories:
Transfection 
Authors:
Smith EL, Finney HM, Nesbitt AM, Ramsdell F, Robinson MK 
In:
Immunology (2006) 119(2): 203-11 
Categories:
Transfection 
Authors:
Dong S, Corre B, Foulon E, Dufour E, Veillette A, Acuto O, Michel F 
In:
J Exp Med (2006) 203(11): 2509-18 
Categories:
Transfection 
Authors:
Remans PH, Wijbrandts CA, Sanders ME, Toes RE, Breedveld FC, Tak PP, van Laar JM, Reedquist KA 
In:
Arthritis Rheum (2006) 54(10): 3135-43 
Categories:
Transfection 
Authors:
Jones KS, Fugo K, Petrow-Sadowski C, Huang Y, Bertolette DC, Lisinski I, Cushman SW, Jacobson S, Ruscetti FW 
In:
J Virol (2006) 80(17): 8291-302 
Categories:
Transfection 
Authors:
Kimura H, Morii E, Ikeda JI, Ezoe S, Xu JX, Nakamichi N, Tomita Y, Shibayama H, Kanakura Y, Aozasa K 
In:
Leukemia (2006) 20(9): 1551-6 
Categories:
Transfection 
Authors:
Pesu M, Muul L, Kanno Y, O'shea JJ 
In:
Blood (2006) 108(3): 983-5 
Categories:
Transfection 
Authors:
Cabrero JR, Serrador JM, Barreiro O, Mittelbrunn M, Naranjo-Suarez S, Martin-Cofreces N, Vicente-Manzanares M, Mazitschek R, Bradner JE, Avila J, Valenzuela-Fernandez A, Sanchez-Madrid F 
In:
Mol Biol Cell (2006) 17(8): 3435-3445 
Categories:
Transfection 
Authors:
Hayashi K, Altman A 
In:
J Immunol (2006) 177(3): 1721-8 
Categories:
Transfection 
Authors:
Espert L, Denizot M, Grimaldi M, Robert-Hebmann V, Gay B, Varbanov M, Codogno P, Biard-Piechaczyk M 
In:
J Clin Invest (2006) 116(8): 2161-72 
Categories:
Transfection 
Authors:
Ohta A, Gorelik E, Prasad SJ, Ronchese F, Lukashev D, Wong MK, Huang X, Caldwell S, Liu K, Smith P, Chen JF, Jackson EK, Apasov S, Abrams S, Sitkovsky M 
In:
Proc Natl Acad Sci USA (2006) 103(35): 13132-7 
Categories:
Transfection 
Authors:
Stallwood Y, Briend E, Ray KM, Ward GA, Smith BJ, Nye E, Champion BR, McKenzie GJ 
In:
J Immunol (2006) 177(2): 885-95 
Categories:
Transfection 
Authors:
Granum S, Sundvold-Gjerstad V, Dai KZ, Kolltveit KM, Hildebrand K, Huitfeldt HS, Lea T, Spurkland A 
In:
BMC Immunol (2006) 7: 15 
Categories:
Transfection 
Authors:
Koguchi K, Anderson DE, Yang L, O'connor KC, Kuchroo VK, Hafler DA 
In:
J Exp Med (2006) 203(6): 1413-8 
Categories:
Transfection 
Authors:
von Essen M, Nielsen MW, Bonefeld CM, Boding L, Larsen JM, Leitges M, Baier G, Odum N, Geisler C 
In:
J Immunol (2006) 176(12): 7502-10 
Categories:
Transfection 
Authors:
Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A 
In:
Proc Natl Acad Sci USA (2006) 103(20): 7765-70 
Categories:
Transfection 
Authors:
Lettau M, Qian J, Linkermann A, Latreille M, Larose L, Kabelitz D, Janssen O 
In:
Proc Natl Acad Sci USA (2006) 103(15): 5911-6 
Categories:
Transfection 
Authors:
Gavin MA, Torgerson TR, Houston E, Deroos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY 
In:
Proc Natl Acad Sci USA (2006) 103(17): 6659-64 
Categories:
Transfection 
Authors:
Grant C, Oh U, Fugo K, Takenouchi N, Griffith C, Yao K, Newhook TE, Ratner L, Jacobson S 
In:
PLoS Pathog (2006) 2(4): e33 
Categories:
Transfection 
Authors:
Keppler OT, Tibroni N, Venzke S, Rauch S and Fackler OT 
In:
J Leukoc Biol (2006) 79(3): 616-27 
Categories:
Transfection 
Authors:
Mantel PY, Ouaked N, Ruckert B, Karagiannidis C, Welz R, Blaser K, Schmidt-Weber CB 
In:
J Immunol (2006) 176(6): 3593-602 
Categories:
Transfection 
Authors:
Santos SG, Antoniou AN, Sampaio P, Powis SJ, Arosa FA 
In:
J Immunol (2006) 176(5): 2942-9 
Categories:
Transfection 
Authors:
Muthumani K, Choo AY, Zong WX, Madesh M, Hwang DS, Premkumar A, Thieu KP, Emmanuel J, Kumar S, Thompson CB, Weiner DB 
In:
Nat Cell Biol (2006) 8(2): 170-9 
Categories:
Transfection 
Authors:
Barabitskaja O, Foulke JS Jr, Pati S, Bodor J, Reitz MS Jr 
In:
J Leukoc Biol (2006) 79(2): 378-87 
Categories:
Transfection 
Authors:
McKenzie G, Ward G, Stallwood Y, Briend E, Papadia S, Lennard A, Turner M, Champion B, Hardingham GE 
In:
BMC Cell Biol (2006) 7: 10 
Categories:
Transfection 
Authors:
Poppe D, Tiede I, Fritz G, Becker C, Bartsch B, Wirtz S, Strand D, Tanaka S, Galle PR, Bustelo XR and Neurath MF 
In:
J Immunol (2006) 176(1): 640-651 
Categories:
Transfection 
Authors:
Hu S, Du MQ, Park SM, Alcivar A, Qu L, Gupta S, Tang J, Baens M, Ye H, Lee TH, Marynen P, Riley JL and Yang X 
In:
J Clin Invest (2006) 116(1): 174-181 
Categories:
Transfection 
Authors:
Vincent P, Collette Y, Marignier R, Vuaillat C, Rogemond V, Davoust N, Malcus C, Cavagna S, Gessain A, Machuca-Gayet I, Belin MF, Quach T and Giraudon P 
In:
J Immunol (2005) 175(11): 7650-7660 
Categories:
Transfection 
Authors:
Brenner D, Golks A, Kiefer F, Krammer PH and Arnold R 
In:
EMBO J (2005) 24(24): 4279-4290 
Categories:
Transfection 
Authors:
Sundrud MS, Vancompernolle SE, Eger KA, Bruno TC, Subramaniam A, Mummidi S, Ahuja SK and Unutmaz D 
In:
Blood (2005) 106(10): 3440-3448 
Categories:
Transfection 
Authors:
Yang J, Castle BE, Hanidu A, Stevens L, Yu Y, Li X, Stearns C, Papov V, Rajotte D and Li J 
In:
J Immunol (2005) 175(10): 6580-6588 
Categories:
Transfection 
Authors:
Miyahara Y, Naota H, Wang L, Hiasa A, Goto M, Watanabe M, Kitano S, Okumura S, Takemitsu T, Yuta A, Majima Y, Lemonnier FA, Boon T and Shiku H 
In:
Clin Cancer Res (2005) 11(15): 5581-5589 
Categories:
Transfection 
Authors:
Radons J, Gross C, Stangl S and Multhoff G 
In:
J Immunol Methods (2005) 303(1-2): 135-141 
Categories:
Transfection 
Authors:
Garcia-Bernal D, Wright N, Sotillo-Mallo E, Nombela-Arrieta C, Stein JV, Bustelo XR and Teixido J 
In:
Mol Biol Cell (2005) 16(7): 3223-3235 
Categories:
Transfection 
Authors:
Schmidt-Weber CB, Letarte M, Kunzmann S, Ruckert B, Bernabeu C and Blaser K 
In:
Int Immunol (2005) 17(7): 921-930 
Categories:
Transfection 
Authors:
Paccani SR, Boncristiano M, Patrussi L, Ulivieri C, Wack A, Valensin S, Hirst TR, Amedei A, Del Prete G, Telford JL, D'Elios MM and Baldari CT 
In:
Blood (2005) 106(2): 626-634 
Categories:
Transfection 
Authors:
Urnov FD, Miller JC, Lee YL, Beausejour CM, Rock JM, Augustus S, Jamieson AC, Porteus MH, Gregory PD and Holmes MC 
In:
Nature (2005) 435(7042): 646-651 
Categories:
Transfection 
Authors:
Uhlin M, Masucci MG and Levitsky V 
In:
Proc Natl Acad Sci USA (2005) 102(26): 9264-9269 
Categories:
Transfection 
Authors:
Shamri R, Grabovsky V, Gauguet JM, Feigelson S, Manevich E, Kolanus W, Robinson MK, Staunton DE, von Andrian UH and Alon R 
In:
Nat Immunol (2005) 6(5): 497-506 
Categories:
Transfection 
Authors:
Zhao H, Li CC, Pardo J, Chu PC, Liao CX, Huang J, Dong JG, Zhou X, Huang Q, Huang B, Bennett MK, Molineaux SM, Lu H, Daniel-Issakani S, Payan DG and Masuda ES 
In:
J Immunol (2005) 174(9): 5288-5297 
Categories:
Transfection 
Authors:
Yamano Y, Takenouchi N, Li HC, Tomaru U, Yao K, Grant CW, Maric DA and Jacobson S 
In:
J Clin Invest (2005) 115(5): 1361-1368 
Categories:
Transfection 
Authors:
Kaminuma O, Mori A, Kitamura N, Hashimoto T, Kitamura F, Inokuma S, Miyatake S 
In:
Int Arch Allergy Immunol (2005) 137 Suppl 1: 55-9 
Categories:
Transfection 
Authors:
Juang YT, Wang Y, Solomou EE, Li Y, Mawrin C, Tenbrock K, Kyttaris VC and Tsokos GC 
In:
J Clin Invest (2005) 115(4): 996-1005 
Categories:
Transfection 
Authors:
Keppler OT, Allespach I, Schuller L, Fenard D, Greene WC and Fackler OT 
In:
J Virol (2005) 79(3): 1655-1665 
Categories:
Transfection 
Authors:
Barda-Saad M, Braiman A, Titerence R, Bunnell SC, Barr VA and Samelson LE 
In:
Nat Immunol (2005) 6(1): 80-89 
Categories:
Transfection 
Authors:
Kusaba H, Ghosh P, Derin R, Buchholz M, Sasaki C, Madara K and Longo DL 
In:
J Biol Chem (2005) 280(2): 1037-1043 
Categories:
Transfection 
Authors:
Ptasznik A, Nakata Y, Kalota A, Emerson SG and Gewirtz AM 
In:
Nat Med (2004) 10(11): 1187-1189 
Categories:
Transfection 
Authors:
Okamoto N, Nukada Y, Tezuka K, Ohashi K, Mizuno K and Tsuji T 
In:
Int Immunol (2004) 16(10): 1515-1522 
Categories:
Transfection 
Authors:
Nagaraj S, Ziske C and Schmidt-Wolf IG 
In:
Genet Vaccines Ther (2004) 2(1): 12 
Categories:
Transfection 
Authors:
Muppidi JR and Siegel RM 
In:
Nat Immunol (2004) 5(2): 182-189 
Categories:
Transfection 
Authors:
Feldmann J, Callebaut I, Raposo G, Certain S, Bacq D, Dumont C, Lambert N, Ouachee-Chardin M, Chedeville G, Tamary H, Minard-Colin V, Vilmer E, Blanche S, Le Deist F, Fischer A and de Saint Basile G 
In:
Cell (2003) 115(4): 461-473 
Categories:
Serum-free and Speciality Media 
Authors:
Laport GG, Levine BL, Stadtmauer EA, Schuster SJ, Luger SM, Grupp S, Bunin N,  Strobl FJ, Cotte J, Zheng Z, Gregson B, Rivers P, Vonderheide RH, Liebowitz DN, Porter DL, June CH. 
In:
Blood (2003) 102 (6): 2004-13 
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