Proprotein convertase furin is preferentially expressed in T helper 1 cells and regulates interferon-

Pesu M, Muul L, Kanno Y, O'shea JJ
Source: Blood
Publication Date: (2006)
Issue: 108(3): 983-5
Research Area:
Immunotherapy / Hematology
Cells used in publication:
T cell, human peripheral blood unstim.
Species: human
Tissue Origin: blood
T cell, human stim.
Species: human
Tissue Origin: blood
Nucleofectorâ„¢ I/II/2b
Interleukin (IL) -12 is a major inducer of interferon gamma (IFN-gamma) and the principal mediator of T helper (Th)1 differentiation. To identify IL-12-regulated genes, which might contribute to Th1 differentiation and IFNG regulation, we employed microarray analysis. Surprisingly, a ubiquitously expressed proprotein convertase (PC) furin, was one of the most consistently IL-12-induced genes in T cells and among PCs was the only one regulated by this cytokine. Furin was preferentially expressed in differentiated Th1 cells in a Stat4-dependent manner. Expression of furin enhanced IFN-gamma secretion, whereas inhibition of furin interfered with IFN-gamma production. Thus, we conclude that IL-12 induction of furin might represent a new aspect of IFN-gamma regulation and control of Th1 differentiation.