Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function

Authors:
Kubach J, Lutter P, Bopp T, Stoll S, Becker C, Huter E, Richter C, Weingarten P, Warger T, Knop J, Mullner S, Wijdenes J, Schild H, Schmitt E, Jonuleit H
In:
Source: Blood
Publication Date: (2007)
Issue: 110(5): 1550-8
Research Area:
Immunotherapy / Hematology
Cells used in publication:
T cell, human peripheral blood unstim.
Species: human
Tissue Origin: blood
Culture Media:
Platform:
Nucleofectorâ„¢ I/II/2b
Experiment

Isolation of CD4+CD25+ regulatory T-cells from PBMCs using X-VIVO15. 

Abstract

CD4(+)CD25(+)Foxp3(+) regulatory T cells (CD25(+) Tregs) direct the maintenance of immunological self-tolerance by active suppression of autoaggressive T cell populations. However, the molecules mediating the anergic state and regulatory function of CD25(+) Tregs are still elusive. Using differential proteomics, we identified galectin-10, a member of the lectin family, as constitutively expressed in human CD25(+) Tregs while nearly absent in resting and activated CD4(+) T cells. These data were confirmed on mRNA and protein level. Single cell staining and flow cytometry showed a strictly intracellular expression of galectin-10 in CD25(+) Tregs. Specific inhibition of galectin-10 restored the proliferative capacity of CD25(+) Tregs and abrogated their suppressive function. Notably, first identified here as expressed in human T lymphocytes, galectin-10 is essential for the functional properties of CD25(+) Tregs.