Human CD4+CD25+ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function

Kubach J, Lutter P, Bopp T, Stoll S, Becker C, Huter E, Richter C, Weingarten P, Warger T, Knop J, Mullner S, Wijdenes J, Schild H, Schmitt E, Jonuleit H
Source: Blood
Publication Date: (2007)
Issue: 110(5): 1550-8
Research Area:
Immunotherapy / Hematology
Cells used in publication:
T cell, human peripheral blood unstim.
Species: human
Tissue Origin: blood
Culture Media:
Nucleofector® I/II/2b

Isolation of CD4+CD25+ regulatory T-cells from PBMCs using X-VIVO15. 


CD4(+)CD25(+)Foxp3(+) regulatory T cells (CD25(+) Tregs) direct the maintenance of immunological self-tolerance by active suppression of autoaggressive T cell populations. However, the molecules mediating the anergic state and regulatory function of CD25(+) Tregs are still elusive. Using differential proteomics, we identified galectin-10, a member of the lectin family, as constitutively expressed in human CD25(+) Tregs while nearly absent in resting and activated CD4(+) T cells. These data were confirmed on mRNA and protein level. Single cell staining and flow cytometry showed a strictly intracellular expression of galectin-10 in CD25(+) Tregs. Specific inhibition of galectin-10 restored the proliferative capacity of CD25(+) Tregs and abrogated their suppressive function. Notably, first identified here as expressed in human T lymphocytes, galectin-10 is essential for the functional properties of CD25(+) Tregs.