U-937

human histiocytic lymphoma, promonocytic, T cell like

Cell Type:
Monocyte
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and human or recombinant transferrin.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

HL-1™ Serum-free Medium Supplement (100X) is a medium additive that can be used to replace serum or significantly reduce its concentration in a variety of basal media.
It contains less than 30 µg protein/ml when diluted 1:100 in medium and it does not contain bovine serum albumin.

Storage = 15°C to 30°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF

EP-100

2e5 32-39% 85% Plasmid (general) 0.2 µg 20 µl 4D X-Unit
C W-001 1e6 66.9% ±2 84.9% ±1 Plasmid (general) 2 µg 100 µl I/II/2b
SF 96-EP-100 2e5 36% ±3 85% ±4 Plasmid (general) 0.2 µg 20 µl Shuttle

Citations (45)

Categories:
Primary Cells and Media 
Authors:
Wichmann C, Quagliano-Lo Coco I, Yildiz Ö, Chen-Wichmann L, Weber H, Syzonenko T, Döring C, Brendel C, Ponnusamy K, Kinner A, Brandts C, Henschler R, Grez M 
In:
Leukemia (2015) 29(2): 279-89 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lee JH, Sohn JH, Ryu SY, Hong CS, Moon KD, Park JW 
In:
J Cell Mol Med (2013) 17(10): 1271-81 
Categories:
Transfection 
Authors:
Chung J, Serezani CH, Huang SK, Stern JN, Keskin DB, Jagirdar R, Brock TG, Aronoff DM, Peters-Golden M 
In:
J Immunol (2008) 181(8): 5501-9 
Categories:
Transfection 
Authors:
Tachado SD, Li X, Swan K, Patel N, Koziel H 
In:
J Biol Chem (2008) 283(48): 33191-8 
Categories:
Transfection 
Authors:
Dolniak B, Katsoulidis E, Carayol N, Altman JK, Redig AJ, Tallman MS, Ueda T, Watanabe-Fukunaga R, Fukunaga R, Platanias LC 
In:
J Biol Chem (2008) 283(18): 12034-42 
Categories:
Transfection 
Authors:
Beekman JM, van der Poel CE, van der Linden JA, van den Berg DL, van den Berghe PV, van de Winkel JG, Leusen JH 
In:
J Immunol (2008) 180(6): 3938-45 
Categories:
Transfection 
Authors:
Kim K, Kim HH, Kim JH, Choi YH, Kim YH, Cheong J 
In:
J Leukoc Biol (2007) 82(5): 1332-9 
Categories:
Transfection 
Authors:
Geletu M, Balkhi MY, Peer Zada AA, Christopeit M, Anto Pulikkan J, Kumar Trivedi A, Tenen DG, Behre G 
In:
Blood (2007) 110(9): 3301-9 
Categories:
Transfection 
Authors:
Vogel CF, Li W, Sciullo E, Newman J, Hammock B, Reader JR, Tuscano J, Matsumura F 
In:
Am J Pathol (2007) 171(5): 1538-48 
Categories:
Transfection 
Authors:
Gattenloehner S, Chuvpilo S, Langebrake C, Reinhardt D, Muller-Hermelink HK, Serfling E, Vincent A, Marx A 
In:
Blood (2007) 110(6): 2027-33 
Categories:
Transfection 
Authors:
Ray PS, Fox PL 
In:
EMBO J (2007) 26(14): 3360-72 
Categories:
Transfection 
Authors:
Goodall JC, Ellis L, Yeo GS, Gaston JS 
In:
Rheumatology (2007) 46(2): 232-7 
Categories:
Transfection 
Authors:
Kim JS, Eom JI, Cheong JW, Choi AJ, Lee JK, Yang WI, Min YH 
In:
Clin Cancer Res (2007) 13(3): 1019-28 
Categories:
Transfection 
Authors:
Zhang J, Wang S, Kern S, Cui X, Danner RL 
In:
J Biol Chem (2007) 282(2): 1003-9 
Categories:
Transfection 
Authors:
Chen S, Dai Y, Harada H, Dent P, Grant S 
In:
Cancer Res (2007) 67(2): 782-91 
Categories:
Transfection 
Authors:
Kapasi P, Chaudhuri S, Vyas K, Baus D, Komar AA, Fox PL, Merrick WC, Mazumder B 
In:
Mol Cell (2007) 25(1): 113-26 
Categories:
Transfection 
Authors:
Zada AA, Pulikkan JA, Bararia D, Geletu M, Trivedi AK, Balkhi MY, Hiddemann WD, Tenen DG, Behre HM, Behre G 
In:
Leukemia (2006) 20(12): 2137-2146 
Categories:
Transfection 
Authors:
Fiskus W, Pranpat M, Balasis M, Herger B, Rao R, Chinnaiyan A, Atadja P, Bhalla K 
In:
Mol Cancer Ther (2006) 5(12): 3096-104 
Categories:
Transfection 
Authors:
Keeshan K, He Y, Wouters BJ, Shestova O, Xu L, Sai H, Rodriguez CG, Maillard I, Tobias JW, Valk P, Carroll M, Aster JC, Delwel R, Pear WS 
In:
Cancer Cell (2006) 10(5): 401-11 
Categories:
Transfection 
Authors:
Fernandez-Vidal A, Ysebaert L, Didier C, Betous R, De Toni F, Prade-Houdellier N, Demur C, Contour-Galcera MO, Prevost GP, Ducommun B, Payrastre B, Racaud-Sultan C, Manenti S 
In:
Cancer Res (2006) 66(14): 7128-35 
Categories:
Transfection 
Authors:
De Toni F, Racaud-Sultan C, Chicanne G, Mas VM, Cariven C, Mesange F, Salles JP, Demur C, Allouche M, Payrastre B, Manenti S, Ysebaert L 
In:
Oncogene (2006) 25(22): 3113-3122 
Categories:
Transfection 
Authors:
Bellodi C, Kindle K, Bernassola F, Dinsdale D, Cossarizza A, Melino G, Heery D, Salomoni P 
In:
J Biol Chem (2006) 281(20): 14465-14473 
Categories:
Transfection 
Authors:
Sancho P, Fernandez C, Yuste VJ, Amran D, Ramos AM, de Blas E, Susin SA, Aller P 
In:
Apoptosis (2006) 11(5): 673-86 
Categories:
Transfection 
Authors:
Yoshimi R, Yamaji S, Suzuki A, Mishima W, Okamura M, Obana T, Matsuda C, Miwa Y, Ohno S, Ishigatsubo Y 
In:
J Immunol (2006) 176(6): 3611-24 
Categories:
Transfection 
Authors:
Perez R, Balboa MA and Balsinde J 
In:
J Immunol (2006) 176(4): 2555-2561 
Categories:
Transfection 
Authors:
Muthumani K, Choo AY, Zong WX, Madesh M, Hwang DS, Premkumar A, Thieu KP, Emmanuel J, Kumar S, Thompson CB, Weiner DB 
In:
Nat Cell Biol (2006) 8(2): 170-9 
Categories:
Transfection 
Authors:
Gao N, Rahmani M, Shi X, Dent P and Grant S 
In:
Blood (2006) 107(1): 241-249 
Categories:
Transfection 
Authors:
de Bouteiller O, Merck E, Hasan UA, Hubac S, Benguigui B, Trinchieri G, Bates EE and Caux C 
In:
J Biol Chem (2005) 280(46): 38133-38145 
Categories:
Transfection 
Authors:
Dublet B, Ruello A, Pederzoli M, Hajjar E, Courbebaisse M, Canteloup S, Reuter N and Witko-Sarsat V 
In:
J Biol Chem (2005) 80(34): 30242-30253 
Categories:
Transfection 
Authors:
Helbling D, Mueller BU, Timchenko NA, Schardt J, Eyer M, Betts DR, Jotterand M, Meyer-Monard S, Fey MF and Pabst T 
In:
Blood (2005) 106(4): 1369-1375 
Categories:
Transfection 
Authors:
Drakesmith H, Chen N, Ledermann H, Screaton G, Townsend A and Xu XN 
In:
Proc Natl Acad Sci USA (2005) 102(31): 11017-11022 
Categories:
Transfection 
Authors:
Gao N, Rahmani M, Dent and Grant S 
In:
Oncogene (2005) 24(23): 3797-3809 
Categories:
Transfection 
Authors:
Hahn M, Li W, Yu C, Rahmani M, Dent P and Grant S 
In:
Mol Cancer Ther (2005) 4(3): 457-470 
Categories:
Transfection 
Authors:
Rahmani M, Reese E, Dai Y, Bauer C, Payne SG, Dent P, Spiegel S and Grant S 
In:
Cancer Res (2005) 65(6): 2422-2432 
Categories:
Transfection 
Authors:
Dai Y, Rahmani M, Pei XY, Khanna P, Han SI, Mitchell C, Dent P, Grant S 
In:
Blood (2005) 105(4): 1706-1716 
Categories:
Transfection 
Authors:
Marriott HM, Bingle CD, Read RC, Braley KE, Kroemer G, Hellewell PG, Craig RW, Whyte MK and Dockrell DH 
In:
J Clin Invest (2005) 115(2): 359-368 
Categories:
Transfection 
Authors:
Petit I, Goichberg P, Spiegel A, Peled A, Brodie C, Seger R, Nagler A, Alon R and Lapidot T 
In:
J Clin Invest (2005) 115(1): 168-176 
Categories:
Transfection 
Authors:
Zhao KW, Li X, Zhao Q, Huang Y, Li D, Peng ZG, Shen WZ, Zhao J, Zhou Q, Chen Z, Sims PJ, Wiedmer T and Chen GQ 
In:
Blood (2004) 104(12): 3731-3738 
Categories:
Transfection 
Authors:
Barlic J, McDermott DH, Merrell MN, Gonzales J, Via LE and Murphy PM 
In:
J Biol Chem (2004) 279(47): 48520-48534 
Categories:
Transfection 
Authors:
Min YH, Cheong JW, Kim JY, Eom JI, Lee ST, Hahn JS, Ko YW and Lee MH 
In:
Cancer Res (2004) 64(15): 5225-5231 
Categories:
Transfection 
Authors:
Ross SE, Radomska HS, Wu B, Zhang P, Winnay JN, Bajnok L, Wright WS, Schaufele F, Tenen DG and MacDougald OA 
In:
Mol Cell Biol (2004) 24(2): 675-686 
Categories:
Transfection 
Authors:
Martinet W, Schrijvers DM and Kockx MM 
In:
Biotechnol Lett (2003) 25: 1025-1029 
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