cells

T lymphocytes, isolated from mouse spleen.

Cell Type:

T Cell

Tissue Origin:

blood

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

TheraPEAK® X-VIVO®

TheraPEAK® X-VIVO® Media is a high-performing media series providing nutritionally adequate and balanced environments for a variety of hematopoietic cells including:

T Cells
CAR-T cells
Peripheral blood lymphocytes (PBL)
Tumor infiltrating lymphocytes (TIL)
Human monocytes and macrophages
Lymphokine-activated killer cells (LAK)
Hematopoietic stem cells (HSCs)
Dendritic cells (DCs)

Our scalable media are widely cited in scientific publications and proven to reliably work in many cell therapy applications around the world, from research stages to clinical trials to FDA-approved cell therapies. TheraPEAK® Products are manufactured according to GMP standards and can be safely and confidently used in your clinical processes. Our manufacturing sites are FDA registered with an ISO 13485 certified quality management system.

There are two main versions of this media.

TheraPEAK® X-VIVO® 10 Media are optimized for slower growing, less mature cells, such as hematopoietic stem cells

TheraPEAK® X-VIVO® 15 Media are most appropriate for rapidly growing cells, specifically cells of the immune system

TheraPEAK® X-VIVO® 10 Media and 15 Media are also available with recombinant transferrin instead of native human transferrin

Please note: X-VIVO™ 20 is currently available as an RUO grade catalog item.

Storage: 2-8C, protect from light.

Catalog Offerings:

TheraPEAK® X-VIVO® Medium with native transferrin
Cat. No. NA	Cat.CCat. No. EU	Product	Size
BP04-743Q	BEBP04-743Q	TheraPEAK® X-VIVO® 10 Medium without gentamicin and phenol red, contains native transferrin	1 L bottle
BP04-744Q	BEBP04-744Q	TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin	1 L bottle
08-879H 08-879P1	BE08-879H BE08-879P1	TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin	5 L bag 1 L bag
TheraPEAK™ X-VIVO™ Medium with recombinant transferrin
Cat. No. NA	Cat. No. EU	Product	Size
N/A	BEBP02-055Q	TheraPEAK® X-VIVO® 10 Medium with L-glutamine, without gentamicin or phenol red, contains recombinant transferrin	1 L bottle
BP02-054Q BP02-054P1	BEBP02-054Q BEBP02-054P1	TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains recombinant transferrin	1 L bottle 1 L bag

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
P3	96-DN-100	2e6	40-50%	27-37%	Plasmid (general)	0.5 µg	20 µl	96-well
T cell, mouse	X-001	5e6	33-41%	52-58%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
T cell, mouse	X-001	5e6	23-41%	27-51%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
T cell, mouse	X-001	5e6	32-46%	33-37%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
T cell, mouse	X-001	5e6	37-51%	15-21%	Plasmid (general)	2.5 µg	100 µl	I/II/2b
P3	DN-100	2e6	40-50%	27-37%	Plasmid (general)	0.5 µg	20 µl	4D X-Unit

Citations (17)

Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.

Categories:

Primary Cells and Media, Transfection

Authors:

Oh SA, Seki A, Rutz S.

In:

Curr Protoc Immunol (2019) 124(1): e69

Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.

Categories:

Primary Cells and Media, Transfection

Authors:

Seki A, Rutz S.

In:

J Exp Med (2018) 215(3): 985-997

Primary peripheral T cells become susceptible to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)-mediated apoptosis in vitro upon activation and in the presence of dendritic cells

Categories:

Transfection

Authors:

Singh NP, Nagarkatti M, Nagarkatti P

In:

Mol Pharmacol (2008) 73(6): 1722-35

SNPs upstream of the minimal promoter control IL-2 expression and are candidates for the autoimmune disease-susceptibility locus Aod2/Idd3/Eae3

Categories:

Transfection

Authors:

Del Rio R, Noubade R, Subramanian M, Saligrama N, Diehl S, Rincon M, Teuscher C

In:

Genes Immun (2008) 9(2): 115-21

A peptide that ameliorates lupus up-regulates the diminished expression of early growth response factors 2 and 3

Categories:

Transfection

Authors:

Sela U, Dayan M, Hershkoviz R, Lider O, Mozes E

In:

J Immunol (2008) 180(3): 1584-91

Syndecan-4 Mediates the Coinhibitory Function of DC-HIL on T Cell Activation

Categories:

Transfection

Authors:

Chung JS, Dougherty I, Cruz PD Jr, Ariizumi K

In:

J Immunol (2007) 179(9): 5778-84

A biochemical signature for rapid recall of memory CD4 T cells

Categories:

Transfection

Authors:

Chandok MR, Okoye FI, Ndejembi MP, Farber DL

In:

J Immunol (2007) 179(6): 3689-98

OX40 Gene Expression Is Up-Regulated by Chromatin Remodeling in Its Promoter Region Containing Sp1/Sp3, YY1, and NF-B Binding Sites

Categories:

Transfection

Authors:

Tone Y, Kojima Y, Furuuchi K, Brady M, Yashiro-Ohtani Y, Tykocinski ML, Tone M

In:

J Immunol (2007) 179(3): 1760-7

The lymphocyte function-associated antigen-1 receptor costimulates plasma membrane Ras via phospholipase D2

Categories:

Transfection

Authors:

Mor A, Campi G, Du G, Zheng Y, Foster DA, Dustin ML, Philips MR

In:

Nat Cell Biol (2007) 9(6): 713-9

Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression

Categories:

Transfection

Authors:

Bopp T, Becker C, Klein M, Klein-Heßling S, Palmetshofer A, Serfling E, Heib V, Becker M, Kubach J, Schmitt S, Stoll S, Schild H, Staege MS, Stassen M, Jonuleit H, Schmitt E

In:

J Exp Med (2007) 204(6): 1303-1310

Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3

Categories:

Transfection

Authors:

Hosokawa H, Kimura MY, Shinnakasu R, Suzuki A, Miki T, Koseki H, van Lohuizen M, Yamashita M, Nakayama T

In:

J Immunol (2006) 177(11): 7656-64

Nucleofection and adoptive transfer of primary mouse T lymphocytes

Categories:

Transfection

Authors:

Li L, Boussiotis V

In:

Nat Protocols (2006) epub: online

Enrichment of regulatory CD4(+)CD25(+) T cells by inhibition of phospholipase D signaling

Categories:

Transfection

Authors:

Singh N, Seki Y, Takami M, Baban B, Chandler PR, Khosravi D, Zheng X, Takezaki M, Lee JR, Mellor AL, Bollag WB, Iwashima M

In:

Nat Methods (2006) 3(8): 629-36

Absence of the steroid receptor coactivator-3 induces B-cell lymphoma

Categories:

Transfection

Authors:

Coste A, Antal MC, Chan S, Kastner P, Mark M, O'malley BW, Auwerx J

In:

EMBO J (2006) 25(11): 2453-64

Cutting Edge: Itk-Dependent Signals Required for CD4+ T Cells to Exert, but Not Gain, Th2 Effector Function

Categories:

Transfection

Authors:

Au-Yeung BB, Katzman SD, Fowell DJ

In:

J Immunol (2006) 176(7): 3895-9

Coordination of NF-B and NFAT Antagonism by the Forkhead Transcription Factor Foxd1

Categories:

Transfection

Authors:

Lin L, Peng SL

In:

J Immunol (2006) 176(8): 4793-803

Efficient nonviral gene delivery into primary lymphocytes from rats and mice

Categories:

Transfection

Authors:

Goffinet C and Keppler OT

In:

FASEB J (2006) 20(3): 500-2

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T cell, mouse - BALB/c

Recommended Media

Transfection Information

Citations (17)