T cell, mouse - BALB/c

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T lymphocytes, isolated from mouse spleen.

Cell Type:
T Cell
Tissue Origin:
blood
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 96-DN-100 2e6 40-50% 27-37% Plasmid (general) 0.5 µg 20 µl 96-well
T cell, mouse X-001 5e6 33-41% 52-58% Plasmid (general) 2.5 µg 100 µl I/II/2b
T cell, mouse X-001 5e6 23-41% 27-51% Plasmid (general) 2.5 µg 100 µl I/II/2b
T cell, mouse X-001 5e6 32-46% 33-37% Plasmid (general) 2.5 µg 100 µl I/II/2b
T cell, mouse X-001 5e6 37-51% 15-21% Plasmid (general) 2.5 µg 100 µl I/II/2b
P3

DN-100

 2e6 40-50% 27-37% Plasmid (general) 0.5 µg 20 µl 4D X-Unit

Citations (17)

Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Oh SA, Seki A, Rutz S.  
In:
Curr Protoc Immunol (2019) 124(1): e69 
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Seki A, Rutz S. 
In:
J Exp Med (2018) 215(3): 985-997 
Primary peripheral T cells become susceptible to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)-mediated apoptosis in vitro upon activation and in the presence of dendritic cells
Categories:
Transfection 
Authors:
Singh NP, Nagarkatti M, Nagarkatti P 
In:
Mol Pharmacol (2008) 73(6): 1722-35 
SNPs upstream of the minimal promoter control IL-2 expression and are candidates for the autoimmune disease-susceptibility locus Aod2/Idd3/Eae3
Categories:
Transfection 
Authors:
Del Rio R, Noubade R, Subramanian M, Saligrama N, Diehl S, Rincon M, Teuscher C 
In:
Genes Immun (2008) 9(2): 115-21 
A peptide that ameliorates lupus up-regulates the diminished expression of early growth response factors 2 and 3
Categories:
Transfection 
Authors:
Sela U, Dayan M, Hershkoviz R, Lider O, Mozes E 
In:
J Immunol (2008) 180(3): 1584-91 
Syndecan-4 Mediates the Coinhibitory Function of DC-HIL on T Cell Activation
Categories:
Transfection 
Authors:
Chung JS, Dougherty I, Cruz PD Jr, Ariizumi K 
In:
J Immunol (2007) 179(9): 5778-84 
A biochemical signature for rapid recall of memory CD4 T cells
Categories:
Transfection 
Authors:
Chandok MR, Okoye FI, Ndejembi MP, Farber DL 
In:
J Immunol (2007) 179(6): 3689-98 
OX40 Gene Expression Is Up-Regulated by Chromatin Remodeling in Its Promoter Region Containing Sp1/Sp3, YY1, and NF-B Binding Sites
Categories:
Transfection 
Authors:
Tone Y, Kojima Y, Furuuchi K, Brady M, Yashiro-Ohtani Y, Tykocinski ML, Tone M 
In:
J Immunol (2007) 179(3): 1760-7 
The lymphocyte function-associated antigen-1 receptor costimulates plasma membrane Ras via phospholipase D2
Categories:
Transfection 
Authors:
Mor A, Campi G, Du G, Zheng Y, Foster DA, Dustin ML, Philips MR 
In:
Nat Cell Biol (2007) 9(6): 713-9 
Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression
Categories:
Transfection 
Authors:
Bopp T, Becker C, Klein M, Klein-Heßling S, Palmetshofer A, Serfling E, Heib V, Becker M, Kubach J, Schmitt S, Stoll S, Schild H, Staege MS, Stassen M, Jonuleit H, Schmitt E 
In:
J Exp Med (2007) 204(6): 1303-1310 
Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3
Categories:
Transfection 
Authors:
Hosokawa H, Kimura MY, Shinnakasu R, Suzuki A, Miki T, Koseki H, van Lohuizen M, Yamashita M, Nakayama T 
In:
J Immunol (2006) 177(11): 7656-64 
Nucleofection and adoptive transfer of primary mouse T lymphocytes
Categories:
Transfection 
Authors:
Li L, Boussiotis V 
In:
Nat Protocols (2006) epub: online 
Enrichment of regulatory CD4(+)CD25(+) T cells by inhibition of phospholipase D signaling
Categories:
Transfection 
Authors:
Singh N, Seki Y, Takami M, Baban B, Chandler PR, Khosravi D, Zheng X, Takezaki M, Lee JR, Mellor AL, Bollag WB, Iwashima M 
In:
Nat Methods (2006) 3(8): 629-36 
Absence of the steroid receptor coactivator-3 induces B-cell lymphoma
Categories:
Transfection 
Authors:
Coste A, Antal MC, Chan S, Kastner P, Mark M, O'malley BW, Auwerx J 
In:
EMBO J (2006) 25(11): 2453-64 
Cutting Edge: Itk-Dependent Signals Required for CD4+ T Cells to Exert, but Not Gain, Th2 Effector Function
Categories:
Transfection 
Authors:
Au-Yeung BB, Katzman SD, Fowell DJ 
In:
J Immunol (2006) 176(7): 3895-9 
Coordination of NF-B and NFAT Antagonism by the Forkhead Transcription Factor Foxd1
Categories:
Transfection 
Authors:
Lin L, Peng SL 
In:
J Immunol (2006) 176(8): 4793-803 
Efficient nonviral gene delivery into primary lymphocytes from rats and mice
Categories:
Transfection 
Authors:
Goffinet C and Keppler OT 
In:
FASEB J (2006) 20(3): 500-2 
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