Nucleofection and adoptive transfer of primary mouse T lymphocytes
Authors:
Li L, Boussiotis V
In:
Source:
Nat Protocols
Publication Date:
(
2006
)
Issue:
epub
:
online
Research Area:
Immunotherapy / Hematology
Cells used in publication:
T cell, mouse - BALB/c
Species: mouse
Tissue Origin: blood
Platform:
Nucleofector® I/II/2b
Experiment
combined the Amaxa nucleofection technology and adoptive transfer, to successfully knock-down endogenous Smad3 protein by using Smad3 shRNA.
Abstract