Nucleofection and adoptive transfer of primary mouse T lymphocytes

Authors:
Li L, Boussiotis V
In:
Source: Nat Protocols
Publication Date: (2006)
Issue: epub: online
Research Area:
Immunotherapy / Hematology
Cells used in publication:
T cell, mouse - BALB/c
Species: mouse
Tissue Origin: blood
Platform:
Nucleofectorâ„¢ I/II/2b
Experiment
combined the Amaxa nucleofection technology and adoptive transfer, to successfully knock-down endogenous Smad3 protein by using Smad3 shRNA.
Abstract