cells

resident, immunolgical active cell found in tissues under many body surfaces, rich in granules (histamine, heparin...), role in wound healing, defense against pathogens and allergy

Cell Type:

Granulocyte

Tissue Origin:

Species:

human

Research Area:

Dermatology/Tissue Engineering

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Recommended Media

TheraPEAK® X-VIVO®

TheraPEAK® X-VIVO® Media is a high-performing media series providing nutritionally adequate and balanced environments for a variety of hematopoietic cells including:

T Cells
CAR-T cells
Peripheral blood lymphocytes (PBL)
Tumor infiltrating lymphocytes (TIL)
Human monocytes and macrophages
Lymphokine-activated killer cells (LAK)
Hematopoietic stem cells (HSCs)
Dendritic cells (DCs)

Our scalable media are widely cited in scientific publications and proven to reliably work in many cell therapy applications around the world, from research stages to clinical trials to FDA-approved cell therapies. TheraPEAK® Products are manufactured according to GMP standards and can be safely and confidently used in your clinical processes. Our manufacturing sites are FDA registered with an ISO 13485 certified quality management system.

There are two main versions of this media.

TheraPEAK® X-VIVO® 10 Media are optimized for slower growing, less mature cells, such as hematopoietic stem cells

TheraPEAK® X-VIVO® 15 Media are most appropriate for rapidly growing cells, specifically cells of the immune system

TheraPEAK® X-VIVO® 10 Media and 15 Media are also available with recombinant transferrin instead of native human transferrin

Please note: X-VIVO™ 20 is currently available as an RUO grade catalog item.

Storage: 2-8C, protect from light.

Catalog Offerings:

TheraPEAK® X-VIVO® Medium with native transferrin
Cat. No. NA	Cat.CCat. No. EU	Product	Size
BP04-743Q	BEBP04-743Q	TheraPEAK® X-VIVO® 10 Medium without gentamicin and phenol red, contains native transferrin	1 L bottle
BP04-744Q	BEBP04-744Q	TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin	1 L bottle
08-879H 08-879P1	BE08-879H BE08-879P1	TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains native transferrin	5 L bag 1 L bag
TheraPEAK™ X-VIVO™ Medium with recombinant transferrin
Cat. No. NA	Cat. No. EU	Product	Size
N/A	BEBP02-055Q	TheraPEAK® X-VIVO® 10 Medium with L-glutamine, without gentamicin or phenol red, contains recombinant transferrin	1 L bottle
BP02-054Q BP02-054P1	BEBP02-054Q BEBP02-054P1	TheraPEAK® X-VIVO® 15 Medium with L-glutamine, without gentamicin and phenol red, contains recombinant transferrin	1 L bottle 1 L bag

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Efficiency	Viable Cells	Substrate		Format	Platform
Fibroblast, basic	T-020	67%	48%	Plasmid (general)	2 µg	100 µl	I/II/2b
Fibroblast, basic	T-030	83%	40%	Plasmid (general)	2 µg	100 µl	I/II/2b
Fibroblast, basic	X-001	55.8%	64%	Plasmid (general)	2 µg	100 µl	I/II/2b
Fibroblast, basic	X-005	68%	49%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Pharmacological properties of JTE-052: a novel potent JAK inhibitor that suppresses various inflammatory responses in vitro and in vivo.

Categories:

Primary Cells and Media

Authors:

Tanimoto A, Ogawa Y, Oki C, Kimoto Y, Nozawa K, Amano W, Noji S, Shiozaki M, Matsuo A, Shinozaki Y, Matsushita M

In:

Other (2015) 64(1): 41-51

Mast cell, human

Recommended Media

Transfection Information

Citations