IFN regulatory factor-1 negatively regulates CD4+ CD25+ regulatory T cell differentiation by repressing Foxp3 expression
Fragale A, Gabriele L, Stellacci E, Borghi P, Perrotti E, Ilari R, Lanciotti A, Remoli AL, Venditti M, Belardelli F, Battistini A
Immunotherapy / Hematology
Cells used in publication:
T cell, human peripheral blood unstim.
Tissue Origin: blood
T cell, mouse - C57BL/6
Tissue Origin: blood
Primary CD4 T cells in PBS supplemented with 0.5% BSA were incubated overnight at 4°C then 2.5 µg of the Foxp3 promoter luciferase reporter vector, 2 µg of IRF-1 expression vector, 0.2 µg of Renilla luciferase control vector, and 0.5 µg of pEGFP plasmid were added to 5x10^6 CD4 T cells resuspended in 100 µl of Nucleofector solution (Amaxa) and electroporated using the U-014 and X-001 program of the Nucleofector specific for untouched human and murine CD4 T cells, respectively. Transfection efficiency was monitored in all samples by FACS analysis of GFP fluorescence and was ranging from 30 to 50% for human cells depending on the donor in three independent experiments and from 15 to 25% for mouse cells. After 24 h, luciferase activity was measured.
Regulatory T (Treg) cells are critical in inducing and maintaining tolerance. Despite progress in understanding the basis of immune tolerance, mechanisms and molecules involved in the generation of Treg cells remain poorly understood. IFN regulatory factor (IRF)-1 is a pleiotropic transcription factor implicated in the regulation of various immune processes. In this study, we report that IRF-1 negatively regulates CD4(+)CD25(+) Treg cell development and function by specifically repressing Foxp3 expression. IRF-1-deficient (IRF-1(-/-)) mice showed a selective and marked increase of highly activated and differentiated CD4(+)CD25(+)Foxp3(+) Treg cells in thymus and in all peripheral lymphoid organs. Furthermore, IRF-1(-/-) CD4(+)CD25(-) T cells showed extremely high bent to differentiate into CD4(+)CD25(+)Foxp3(+) Treg cells, whereas restoring IRF-1 expression in IRF-1(-/-) CD4(+)CD25(-) T cells impaired their differentiation into CD25(+)Foxp3(+) cells. Functionally, both isolated and TGF-beta-induced CD4(+)CD25(+) Treg cells from IRF-1(-/-) mice exhibited more increased suppressive activity than wild-type Treg cells. Such phenotype and functional characteristics were explained at a mechanistic level by the finding that IRF-1 binds a highly conserved IRF consensus element sequence (IRF-E) in the foxp3 gene promoter in vivo and negatively regulates its transcriptional activity. We conclude that IRF-1 is a key negative regulator of CD4(+)CD25(+) Treg cells through direct repression of Foxp3 expression.
Open in PubMed
©2016 Lonza. All rights reserved.