T cell, human stim.

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Periphal blood lymphocytes; pre-stimulated.

Cell Type:
T Cell
Tissue Origin:
blood
Species:
human
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

X-VIVO

X-VIVO Serum-free Hematopoietic Cell Media provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).These media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are devoid of any protein kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes.

There are three versions of this media:

  • X-VIVO 10  - designed to support the generation of LAK cells in a serum-free environment.
  • X-VIVO 15 - similar in composition to X-VIVO 10 but optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. It also supports the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors, and can be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells as well as the expansion of HUT-78 and related human lymphocytic cell lines.
  • X-VIVO 20 - optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte depleted peripheral blood lymphocytes (PBL) at high density and has also been used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

Storage = 2ºC to 8ºC

04-380Q - X-VIVO 10 with L-glutamine, gentamicin and phenol red
04-743Q - X-VIVO 10 with L-glutamine, but without gentamicin or phenol red
04-418 - X-VIVO 15  with L-glutamine, gentamicin and phenol red
04-744Q - X-VIVO 15 with L-glutamine, but without gentamicin or phenol red
04-448Q - X-VIVO 20  with L-glutamine, gentamicin and phenol red

Lymphocyte Growth Medium-3
LGM-3 is a serum-free medium for lymphocyte and dendritic cell growth
TheraPeak® T-VIVO®

TheraPeak® T-VIVO® Cell Culture Medium is optimized to support cell therapy applications ustilizing human T cells. 

Its novel chemically defined formulation is serum-free and non-animal origin (NAO) to ensure consistency and provide increased process control.  

Unlike other serum-free T-cell culture media, TheraPEAK® T-VIVO®  Cell Culture Medium does not require the addition of human serum (HS) to achieve the desired performance.

It dos not contain cytokines, antibiotics or phenol red

All TheraPEAK® Products are produced according to applicable GMP standards and follow the USP/EP guidance for cell and gene therapy raw materials. TheraPEAK® Media Products are produced at FDA registered manufacturing sites under the auspices of ISO 13485 certified Quality Management Systems.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 EO-115 1e6 63-78% 42-68% Plasmid (general) 0.4 µg 20 µl 384-well
T cell, human T-023 5e6 36-58% Plasmid (general) 1 µg 100 µl I/II/2b
P3

EO-115

 1e6 63-77% 47-72% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 96-EO-115 1e6 63.3-77.3% 47.3-71.3% Plasmid (general) 0.4 µg 20 µl 96-well

Citations (165)

Neoantigen-targeted CD8+ T cell responses with PD-1 blockade therapy
Categories:
Transfection 
Authors:
Cristina Puig-Saus 1 2 3 4, Barbara Sennino 5, Songming Peng 5, Clifford L Wang 5, Zheng Pan 5, Benjamin Yuen 5, Bhamini Purandare 5, Duo An 5, Boi B Quach 5, Diana Nguyen 5, Huiming Xia 6, Sameeha Jilani 7, Kevin Shao 5, Claire McHugh 5, John Greer 5, Phillip Peabody 5, Saparya Nayak 5, Jonathan Hoover 5, Sara Said 5, Kyle Jacoby 5, Olivier Dalmas 5, Susan P Foy 5, Andrew Conroy 5, Michael C Yi 5, Christine Shieh 5, William Lu 5, Katharine Heeringa 5, Yan Ma 5, Shahab Chizari 5, Melissa J Pilling 5, Marc Ting 5, Ramya Tunuguntla 5, Salemiz Sandoval 5, Robert Moot 5, Theresa Hunter 5, Sidi Zhao 6, Justin D Saco 7, Ivan Perez-Garcilazo 7, Egmidio Medina 7, Agustin Vega-Crespo 7, Ignacio Baselga-Carretero 7, Gabriel Abril-Rodriguez 7 8, Grace Cherry 7, Deborah J Wong 7, Jasreet Hundal 6, Bartosz Chmielowski 7 9, Daniel E Speiser 10, Michael T Bethune 5, Xiaoyan R Bao 5, Alena Gros 11, Obi L Griffith 6, Malachi Griffith 6, James R Heath 12, Alex Franzusoff 5, Stefanie J Mandl # 5, Antoni Ribas # 13 14 15 16 
In:
Nature (2023) 8: 2023 
Modular Pooled Discovery of Synthetic Knockin Sequences to Program Durable Cell Therapies
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
F. Blaeschke, Y. Y. Chen, R. Apathy, Z. Li, C. T. Mowery, W.A. Nyberg, A. To, R. Yu, R. Bueno, M. C. Kim, R. Schmidt, D. B. Goodman, T. Feuchtinger, J. Eyquem, C. J. Ye, E. Shifrut, T. L. Roth, A. Marson 
In:
Cell Res (2023) 186(19): :4216-4234.e33 
Improved cytosine base editors generated from TadA variants
Categories:
Transfection 
Authors:
Lam DK, Feliciano PR, Arif A, Bohnuud T, Fernandez TP, Gehrke JM, Grayson P, Lee KD, Ortega MA, Sawyer C, Schwaegerle ND, Peraro L, Young L, Lee SJ, Ciaramella G, Gaudelli NM. 
In:
Nat Cell Biol (2023) 41(5): 686-697 
Clinical Scale Production of Circular mRNA for the Manufacture of Base-Edited CAR T-cell Therapies
Categories:
Transfection 
Authors:
Rosie Woodruff1,2, James Sillibourne1, Martin Pule1,2, Claire Roddie2 
In:
(2023) 01: 01 
Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells
Categories:
Transfection 
Authors:
Glaser V, Flugel C, Kath J, Du W, Drosdek V, Franke C, Stein M, Pruß A, Schmueck-Henneresse M, Volk HD, Reinke P, Wagner DL 
In:
Genome Biology (2023) 24(1): 89 
The CRISPR-Cas12a Platform for Accurate Genome Editing, Gene Disruption, and Efficient Transgene Integration in Human Immune Cells
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Mohr M, Damas N, Gudmand-Høyer J, Zeeberg K, Jedrzejczyk D, Vlassis A, Morera-Gómez M, Pereira-Schoning S, Puš U, Oliver-Almirall A, Lyholm Jensen T, Baumgartner R, Tate Weinert B, Gill RT, Warnecke T 
In:
ACS Nano (2023) 12(2): 375-389 
Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, Allain V, Li Z, Marson A, Eyquem J, Wilson RC 
In:
Nat Biomed Eng (2023) 5: 647-660 
Epitope base editing CD45 in hematopoietic cells enables universal blood cancer immune therapy
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Wellhausen N, O'Connell RP, Lesch S, Engel NW, Rennels AK, Gonzales D, Herbst F, Young RM, Garcia KC, Weiner D, June CH, Gill SI. 
In:
Science (2023) eadi: 1145 
Mitigation of chromosome loss in clinical CRISPR Cas9-engineered T cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Tsuchida CA, Brandes N, Bueno R, Trinidad M, Mazumder T, Yu B, Hwang B, Chang C, Liu J, Sun Y, Hopkins CR, Parker KR, Qi Y, Satpathy AT, Stadtmauer EA, Cate JHD, Eyquem J, Fraietta JA, June CH, Chang HY, Ye CJ, Doudna JA 
In:
Cell Rep (2023) 03.22: 533709 
An evolved AAV variant enables efficient genetic engineering of murine T cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Nyberg WA, Ark J, To A, Clouden S, Reeder G, Muldoon JJ, Chung JY, Xie WH, Allain V, Steinhart Z, Chang C, Talbot A, Kim S, Rosales A, Havlik LP, Pimentel H, Asokan A, Eyquem J 
In:
Cell (2023) 186(2): 446-460 
Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression
Categories:
Transfection 
Authors:
Mueller KP, Piscopo NJ, Forsberg MH, Saraspe LA, Das A, Russell B, Smerchansky M, Cappabianca D, Shi L, Shankar K, Sarko L, Khajanchi N, La Vonne Denne N, Ramamurthy A, Ali A, Lazzarotto CR, Tsai SQ, Capitini CM, Saha K 
In:
J Immunother (2022) 10(9): e004446  
Induced expression of CCL19 promotes the anti-tumor ability of CAR-T cells by increasing their infiltration ability
Categories:
Transfection 
Authors:
Jian-Fei Hu , Zu-Wei Wang , Cheng-Yu Liao , Zhi-Wen Chen , Feng-Ping Kang , Cai-Feng Lin , Tian-Sheng Lin , Long Huang , Yi-Feng Tian , Shi Chen  
In:
Frontiers in Immunology (2022) 13: 958960 
Orthotopic T-cell receptor replacement in primary human T cells using CRISPR-Cas9-mediatedhomology-directed repair
Categories:
Transfection 
Authors:
Carolin Moosmann,* Thomas R. Mueller, Dirk H. Busch, and Kilian Schober 
In:
STAR Protoc (2022) 3: 101031 
CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells
Categories:
Transfection 
Authors:
Petri K, Zhang W, Ma J, Schmidts A, Lee H, Horng JE, Kim DY, Kurt IC, Clement K, Hsu JY, Pinello L, Maus MV, Joung JK, Yeh JJ 
In:
Nat Biotechnol (2022) 40(2): 189-193 
CRISPR-Cas9 based gene editing of the immune checkpoint NKG2A enhances NK cell mediated cytotoxicity against multiple myeloma
Categories:
Transfection 
Authors:
Bexte T, Alzubi J, Reindl LM, Wendel P, Schubert R, Salzmann-Manrique E, von Metzler I, Cathomen T, Ullrich E 
In:
Oncol Rep (2022) 31;11(1): 2081415 
CRISPR-Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Lin-Shiao E, Pfeifer WG, Shy BR, Saffari Doost M, Chen E, Vykunta VS, Hamilton JR, Stahl EC, Lopez DM, Sandoval Espinoza CR, Deyanov AE, Lew RJ, Poirer MG, Marson A, Castro CE, Doudna JA 
In:
Nucleic Acids Res (2022) 50(3): 1256-1268 
High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA
Categories:
Transfection 
Authors:
Oh SA, Senger K, Madireddi S, Akhmetzyanova I, Ishizuka IE, Tarighat S, Lo JH, Shaw D, Haley B, Rutz S 
In:
J Exp Med (2022) 219(5): e20211530 
Pharmacological interventions enhance virus-free generation of TRAC-replaced CAR T cells
Categories:
Transfection 
Authors:
Kath J, Du W, Pruene A, Braun T, Thommandru B, Turk R, Sturgeon ML, Kurgan GL, Amini L, Stein M, Zittel T, Martini S, Ostendorf L, Wilhelm A, Akyüz L, Rehm A, Höpken UE, Pruß A, Künkele A, Jacobi AM, Volk HD, Schmueck-Henneresse M, Stripecke R, Reinke P, Wagner DL 
In:
Mol Ther Methods Clin Dev. (2022) 25: 311-330 
NUDT21 limits CD19 levels through alternativemRNA polyadenylation in B cell acutelymphoblastic leukemia
Categories:
Primary Cells and Media, Transfection 
Authors:
Witkowski MT, Lee S, Wang E, Lee AK, Talbot A, Ma C, Tsopoulidis N, Brumbaugh J, Zhao Y, Roberts KG, Hogg SJ, Nomikou S, Ghebrechristos YE, Thandapani P, Mullighan CG, Hochedlinger K, Chen W, Abdel-Wahab O, Eyquem J, Aifantis I 
In:
Nature (2022) 10: 1424-1432 
Phase 1 clinical trial of CRISPR-engineered CAR19 universal T cells for treatment of children with refractory B cell leukemia
Categories:
Transfection 
Authors:
Ottaviano G, Georgiadis C, Gkazi SA, Syed F, Zhan H, Etuk A, Preece R, Chu J, Kubat A, Adams S, Veys P, Vora A, Rao K, Qasim W; TT52 CRISPR-CAR group. 
In:
Transl Vis Sci Technol (2022) 26;14(668): eabq3010 
Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapy
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Thomas R Müller , Sebastian Jarosch 1, Monika Hammel , Justin Leube , Simon Grassmann  , Bettina Bernard 1, Manuel Effenberger , Immanuel Andrä , M Zeeshan Chaudhry , Theresa Käuferle , Antje Malo , Luka Cicin-Sain , Peter Steinberger , Tobias Feuchtinger , Ulrike Protzer , Kathrin Schumann , Michael Neuenhahn , Kilian Schober , Dirk H Busch  
In:
Cell Rep Med (2021) 2(8): 100374 
Base-edited CAR T Cells for combinational therapy against T cell malignancies
Categories:
Transfection 
Authors:
Christos Georgiadis, Jane Rasaiyaah,  Soragia Athina Gkazi, Roland Preece, Aniekan Etuk, Abraham Christi,  Waseem Qasim 
In:
Leukemia (2021) 35 (12): 3466-3481 
CARAMBA: a first-in-human clinical trial with SLAMF7 CAR-T cells prepared by virus-free Sleeping Beauty gene transfer to treat multiple myeloma
Categories:
Transfection 
Authors:
Sabrina Prommersberger , Michael Reiser , Julia Beckmann , Sophia Danhof , Maximilian Amberger , Patricia Quade-Lyssy , Hermann Einsele , Michael Hudecek , Halvard Bonig , Zoltán Ivics  
In:
Gene Ther (2021) 28: 560-571 
Genome editing of donor-derived T-cells to generate allogenic chimeric antigen receptor-modified T cells: Optimizing aß T cell-depleted haploidentical hematopoietic stem cell transplantation
Categories:
Transfection 
Authors:
Volker Wiebking , Ciaran M Lee , Nathalie Mostrel , Premanjali Lahiri , Rasmus Bak , Gang Bao , Maria Grazia Roncarolo , Alice Bertaina , Matthew H Porteus  
In:
Haematologica (2021) 106(3): 847-858 
CRISPR/Cas9 mediated deletion of the adenosine A2A receptor enhances CAR T cell efficacy
Categories:
Transfection 
Authors:
Giuffrida L, Sek K, Henderson MA, Lai J, Chen AXY, Meyran D, Todd KL, Petley EV, Mardiana S, Mølck C, Stewart GD, Solomon BJ, Parish IA, Neeson PJ, Harrison SJ, Kats LM, House IG, Darcy PK, Beavis PA 
In:
Nat Commun. (2021) 12(1): 3236 
CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
Categories:
Transfection 
Authors:
Kluesner MG, Lahr WS, Lonetree CL, Smeester BA, Qiu X, Slipek NJ, Claudio Vázquez PN, Pitzen SP, Pomeroy EJ, Vignes MJ, Lee SC, Bingea SP, Andrew AA, Webber BR, Moriarity BS 
In:
Nature (2021) 12(1): 2437 
Fast and Efficient Genome Editing of Human FOXP3+ Regulatory T Cells
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Van Zeebroeck L, Arroyo Hornero R, Côrte-Real BF, Hamad I, Meissner TB, Kleinewietfeld M. 
In:
Frontiers in Immunology (2021) 12: 655122 
Phenotype of SARS-CoV-2-specific T-cells in COVID-19 patients with acute respiratory distress syndrome
Categories:
Classical Media and Reagents 
Authors:
Daniela Weiskopf, Katharina S. Schmitz, Matthijs P. Raadsen, Alba Grifoni, Nisreen M.A. Okba, Henrik Endeman, Johannes P.C. van den Akker, Richard Molenkamp, Marion P.G. Koopmans, Eric C.M. van Gorp, Bart L. Haagmans, Rik L. de Swart, Alessandro Sette & Rory D. de Vries 
In:
Science (2020) 5: 48 
CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cells
Categories:
Primary Cells and Media, Transfection 
Authors:
Goodwin M, Lee E, Lakshmanan U, Shipp S, Froessl L, Barzaghi F, Passerini L, Narula M, Sheikali A, Lee CM, Bao G, Bauer CS, Miller HK, Garcia-Lloret M, Butte MJ, Bertaina A, Shah A, Pavel-Dinu M, Hendel A, Porteus M, Roncarolo MG, Bacchetta R. 
In:
Science (2020) 6(19): eaaz0571 
Oncolytic virus-derived type I interferon restricts CAR T cell therapy
Categories:
Transfection 
Authors:
Evgin L, Huff AL, Wongthida P, Thompson J, Kottke T, Tonne J, Schuelke M, Ayasoufi K, Driscoll CB, Shim KG, Reynolds P, Monie DD, Johnson AJ, Coffey M, Young SL, Archer G, Sampson J, Pulido J, Perez LS, Vile R. 
In:
Nat Commun. (2020) Jun 24;11(1): 3187. doi: 10.1038/s41467-020-17011-z 
Detection and Modulation of DNA Translocations During Multi-Gene Genome Editing in T Cells
Categories:
Transfection 
Authors:
Bothmer A, Gareau KW, Abdulkerim HS, Buquicchio F, Cohen L, Viswanathan R, Zuris JA, Marco E, Fernandez CA, Myer VE, Cotta-Ramusino C. 
In:
CRISPR J (2020) Jun;3(3): 177-187 
Abrogation of HLA surface expression using CRISPR/Cas9 genome editing: a step toward universal T cell therapy
Categories:
Transfection 
Authors:
Lee J, Sheen JH, Lim O, Lee Y, Ryu J, Shin D, Kim YY, Kim M. 
In:
Scientific Reports (2020) 20;10(1): :17753 
Functional CRISPR dissection of gene networks controlling human regulatory T cell identity
Categories:
Transfection 
Authors:
Schumann K, Raju SS, Lauber M, Kolb S, Shifrut E, Cortez JT, Skartsis N, Nguyen VQ, Woo JM, Roth TL, Yu R, Nguyen MLT, Simeonov DR, Nguyen DN, Targ S, Gate RE, Tang Q, Bluestone JA, Spitzer MH, Ye CJ, Marson A. 
In:
Nat Immunol (2020) (11): 1456-1466 
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
Categories:
Transfection 
Authors:
Wienert B, Nguyen DN, Guenther A, Feng SJ, Locke MN, Wyman SK, Shin J, Kazane KR, Gregory GL, Carter MAM, Wright F, Conklin BR, Marson A, Richardson CD, Corn JE 
In:
Nat Commun. (2020) 11(1): 2109 
Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies
Categories:
Transfection 
Authors:
Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A 
In:
Cell Res (2020) 181(3): 728-744.e21 
Large-scale GMP-compliant CRISPR-Cas9–mediated deletion of the glucocorticoid receptor in multivirus-specific T cells
Categories:
Transfection 
Authors:
Basar R, Daher M, Uprety N, Gokdemir E, Alsuliman A, Ensley E, Ozcan G, Mendt M, Hernandez Sanabria M, Kerbauy LN, Nunez Cortes AK, Li L, Banerjee PP, Muniz-Feliciano L, Acharya S, Fowlkes NW, Lu J, Li S, Mielke S, Kaplan M, Nandivada V, Bdaiwi M, Kontoyiannis AD, Li Y, Liu E, Ang S, Marin D, Brunetti L, Gundry MC, Turk R, Schubert MS, Rettig GR, McNeill MS, Kurgan G, Behlke MA, Champlin R, Shpall EJ, Rezvani K. 
In:
Blood (2020) 4(14): 3357-3367 
CRISPR-targeted display of functional T cell receptors enables 2 engineering of enhanced specificity and prediction of cross-reactivity
Categories:
Transfection 
Authors:
Rodrigo Vazquez-Lombardi, Johanna S. Jung, Florian Bieberich, Edo Kapetanovic, Erik Aznauryan, Cédric R. Weber and Sai T. Reddy 
In:
BioResearch Open Access (2020) :  
Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency.
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Nguyen DN, Roth TL, Li PJ, Chen PA, Apathy R, Mamedov MR, Vo LT, Tobin VR, Goodman D, Shifrut E, Bluestone JA, Puck JM, Szoka FC, Marson A. 
In:
Nat Biotechnol (2019) 1: 1 
Gene correction for SCID-X1 in long-term hematopoietic stem cells.
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Pavel-Dinu M, Wiebking V, Dejene BT, Srifa W, Mantri S, Nicolas CE, Lee C, Bao G, Kildebeck EJ, Punjya N, Sindhu C, Inlay MA, Saxena N, DeRavin SS, Malech H, Roncarolo MG, Weinberg KI, Porteus MH 
In:
Nat Commun. (2019) 10 (1): 1634 
Orthotopic replacement of T-cell receptor a- and ß-chains with preservation of near-physiological T-cell function.
Categories:
Primary Cells and Media, Transfection 
Authors:
Schober K, Müller TR, Gökmen F, Grassmann S, Effenberger M, Poltorak M, Stemberger C, Schumann K, Roth TL, Marson A, Busch DH. 
In:
Nat Biomed Eng (2019) 10: 01 
Ribonucleoprotein Transfection for CRISPR/Cas9-Mediated Gene Knockout in Primary T Cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Oh SA, Seki A, Rutz S.  
In:
Curr Protoc Immunol (2019) 124(1): e69 
Engineering of CRISPR-Cas12b for human genome editing.
Categories:
Transfection 
Authors:
Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F. 
In:
Nature (2019) 10(1): 212 
c-Jun overexpression in CAR T cells induces exhaustion resistance
Categories:
Transfection 
Authors:
Lynn RC, Weber EW, Sotillo E, Gennert D, Xu P, Good Z, Anbunathan H, Lattin J, Jones R, Tieu V, Nagaraja S, Granja J, de Bourcy CFA, Majzner R, Satpathy AT, Quake SR, Monje M, Chang HY, Mackall CL 
In:
Nature (2019) 576(7786): 293-300 
CRISPR-Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV-host factor interactions.
Categories:
Primary Cells and Media, Transfection 
Authors:
Hultquist JF, Hiatt J, Schumann K, McGregor MJ, Roth TL, Haas P, Doudna JA, Marson A, Krogan NJ.  
In:
Nat Protocols (2019) 14(1): 1-27 
Bacteria-free minicircle DNA system to generate integration-free CAR-T cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Chen Cheng, Na Tang, Jiaxin Li, Shiwei Cao, Tongtong Zhang, Xiaofei Wei, Haoyi Wang 
In:
J Med Genetics (2019) 56: 10–17 
Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Ng MSF, Roth TL, Mendoza VF, Marson A, Burt TD 
In:
Science (2019) 4(41): eaav5947 
Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function.
Categories:
Transfection 
Authors:
Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME,  Ashworth A, Marson A.  
In:
Cell (2018) 175(7): 1985-1971 
Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cells.
Categories:
Transfection 
Authors:
Ting PY, Parker AE, Lee JS, Trussell C, Sharif O, Luna F, Federe G, Barnes SW, Walker JR, Vance J, Gao MY, Klock HE, Clarkson S, Russ C, Miraglia LJ, Cooke MP, Boitano AE, McNamara P, Lamb J, Schmedt C, Snead JL. 
In:
Nat Methods (2018) 15(11):  
 Cytokines induced killer cells produced in good manufacturing practices conditions: identification of the most advantageous and safest expansion method in terms of viability, cellular growth and identity.
Categories:
Serum-free and Speciality Media 
Authors:
Castiglia S, Adamini A, Rustichelli D, Castello L, Mareschi K, Pinnetta G, Leone M, Mandese A, Ferrero I, Mesiano G, Fagioli F.  
In:
J Transl Med (2018) 16: 237 
A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Vakulskas CA, Dever DP, Rettig GR, Turk R, Jacobi AM, Collingwood MA, Bode NM, McNeill MS, Yan S, Camarena J, Lee CM, Park SH, Wiebking V, Bak RO, Gomez-Ospina N, Pavel-Dinu M, Sun W6, Bao G, Porteus MH, Behlke MA 
In:
Nat Med (2018) 24(8): 1216-1224 
Reprogramming human T cell function and specificity with non-viral genome targeting.
Categories:
Primary Cells and Media, Transfection, Laboratory Instrumentation  
Authors:
Roth TL, Puig-Saus C, Yu R, Shifrut E, Carnevale J, Li PJ, Hiatt J, Saco J, Krystofinski P, Li H, Tobin V, Nguyen DN, Lee MR, Putnam AL, Ferris AL, Chen JW, Schickel JN, Pellerin L, Carmody D, Alkorta-Aranburu G, Del Gaudio D, Matsumoto H, Morell M, Mao Y, Cho M, Quadros RM, Gurumurthy CB, Smith B, Haugwitz M, Hughes SH, Weissman JS, Schumann K, Esensten JH, May AP, Ashworth A, Kupfer GM, Greeley SAW, Bacchetta R, Meffre E, Roncarolo MG, Romberg N, Herold KC, Ribas A, Leonetti MD, Marson A. 
In:
Nature (2018) 559: 405-9 
Nucleofection with Plasmid DNA for CRISPR/Cas9-Mediated Inactivation of Programmed Cell Death Protein 1 in CD133-Specific CAR T Cells
Categories:
Transfection 
Authors:
Hu B, Zou Y, Zhang L, Tang J, Niedermann G, Firat E, Huang X, Zhu X.  
In:
Hum Gene Ther (2018) :  
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.
Categories:
Primary Cells and Media, Transfection 
Authors:
Seki A, Rutz S. 
In:
J Exp Med (2018) 215(3): 985-997 
Preclinical modeling highlights the therapeutic potential of hematopoietic stem cell gene editing for correction of SCID-X1
Categories:
Transfection 
Authors:
Schiroli G, Ferrari S, Conway A, Jacob A, Capo V, Albano L, Plati T, Castiello MC, Sanvito F, Gennery AR, Bovolenta C, Palchaudhuri R, Scadden DT, Holmes MC, Villa A, Sitia G, Lombardo A, Genovese P, Naldini L 
In:
Science (2017) 9(411): eaan0820 
Improved Expansion and In Vivo Function of Patient T Cells by a Serum-free Medium.
Categories:
Serum-free and Speciality Media 
Authors:
Medvec AR, Ecker C, Kong H, Winters EA, Glover J, Varela-Rohena A, Riley JL.  
In:
Mol Ther Methods Clin Dev. (2017) 7; 8: 65-74 
Going non-viral: the Sleeping Beauty transposon system breaks on through to the clinical side.
Categories:
Transfection 
Authors:
Hudecek M1, Izsvák Z2, Johnen S3, Renner M4, Thumann G5, Ivics Z4. 
In:
Clin Exp Immunol (2017) 52(4): 355(80) 
CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors.
Categories:
Transfection 
Authors:
Bak RO, Porteus MH. 
In:
Cell Rep (2017) 20(3): 750-756 
CRISPR-Cas9 mediated LAG-3 disruption in CAR-T cells.
Categories:
Transfection 
Authors:
Zhang Y, Zhang X, Cheng C, Mu W, Liu X, Li N, Wei X, Liu X, Xia C, Wang H. 
In:
Frontiers in Immunology (2017) 1: 1-9 
CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells.
Categories:
Transfection 
Authors:
Rupp LJ1, Schumann K, Roybal KT, Gate RE, Ye CJ, Lim WA, Marson A. 
In:
Scientific Reports (2017) 7 (1): 737 
A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, Piechocka-Trocha , Krogan NJ, Marson A, Sabatini DM, Lander ES, Hacohen N, Walker BD. 
In:
Nat Genet (2017) 49(2): 193-203 
A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.
Categories:
Transfection 
Authors:
Hultquist JF, Schumann K, Woo JM, Manganaro L, McGregor MJ, Doudna J, Simon V, Krogan NJ, Marson A. 
In:
Cell Rep (2016) 17(5): 1438-1452 
Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing.
Categories:
Transfection 
Authors:
Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. 
In:
Cell (2016) 167(1): 219-232 
The Activation-Induced Assembly of an RNA/Protein Interactome Centered on the Splicing Factor U2AF2 Regulates Gene Expression in Human CD4 T Cells
Categories:
Transfection 
Authors:
Thomas C. Whisenant, Eigen R. Peralta, Lauren D. Aarreberg, Nina J. Gao, Steven R. Head, Phillip Ordoukhanian, Jamie R. Williamson, Daniel R. Salomon 
In:
PLoS ONE (2015) 10(12): e0144409 
Type 1 diabetes immunotherapy using polyclonal regulatory T cells.
Categories:
Primary Cells and Media, Serum-free and Speciality Media 
Authors:
Bluestone JA1, Buckner JH2, Fitch M3, Gitelman SE4, Gupta S2, Hellerstein MK3, Herold KC5, Lares A6, Lee MR6, Li K7, Liu W6, Long SA2, Masiello LM6, Nguyen V8, Putnam AL6, Rieck M6, Sayre PH9, Tang Q8. 
In:
Med. Sci. (2015) 7: 315 
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Transfection 
Authors:
Ayal Hendel, Rasmus O Bak, Joseph T Clark, Andrew B Kennedy, Daniel E Ryan, Subhadeep Roy, Israel Steinfeld, Benjamin D Lunstad, Robert J Kaiser, Alec B Wilkens, Rosa Bacchetta, Anya Tsalenko, Douglas Dellinger, Laurakay Bruhn, Matthew H Porteus 
In:
Nat Biotechnol (2015) 10.1038: nbt.3290 
Comparison of Lentiviral and Sleeping Beauty Mediated alpha beta T Cell Receptor Gene Transfer
Categories:
Transfection 
Authors:
Anne-Christine Field, Conrad Vink, Richard Gabriel, Roua Al-Subki, Manfred Schmidt, Nicholas Goulden, Hans Stauss, Adrian Thrasher, Emma Morris, Waseem Qasim 
In:
PLoS ONE (2013) 8(6): e68201 
Development of a high-throughput cell-based assay for identification of IL-17 inhibitors
Categories:
 
Authors:
Dobritsa SV, Kuok IT, Nguyen H, Webster JC, Spragg AM, Morley T, Carr GJ. 
In:
J Biomol Screen (2013) 18(1): 75-84 
Inhibition of HSV-1 by chemoattracted neutrophils: supernatants of corneal epithelial cells (HCE) and macrophages (THP-1) treated with virus components chemoattract neutrophils (PMN), and supernatants of PMN treated with these conditioned media inhibit viral growth
Categories:
 
Authors:
Hayashi K, Hooper LC, Okuno T, Takada Y, Hooks JJ 
In:
Virology (2012) 157(7): 1377-81 
A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR.
Categories:
Transfection 
Authors:
Torikai H, Reik A, Liu PQ, Zhou Y, Zhang L, Maiti S, Huls H, Miller JC, Kebriaei P, Rabinovitch B, Lee DA, Champlin RE, Bonini C, Naldini L, Rebar EJ, Gregory PD, Holmes MC, Cooper LJ. 
In:
Blood (2012) 119(24): 5697-5705 
Saftey and persistence of adoptively transferred autologous CD19-targeted T cells in patients with relapsed or chemotherapy refractory B-cell leukemias
Categories:
Serum-free and Speciality Media 
Authors:
Renier J. Brentjens, Isabelle Rivière, Jae H. Park, Marco L. Davila, Xiuyan Wang, Jolanta Stefanski, Clare Taylor, Raymond Yeh, Shirley Bartido, Oriana Borquez-Ojeda, Malgorzata Olszewska, Yvette Bernal, Hollie Pegram, Mark Przybylowski, Daniel Hollyman, Yelena Usachenko, Domenick Pirraglia, James Hosey, Elmer Santos, Elizabeth Halton, Peter Maslak, David Scheinberg, Joseph Jurcic, Mark Heaney, Glenn Heller, Mark Frattini, Michel Sadelain 
In:
Blood (2011) 118 (18): 4817-4828 
Pregnancy-specific glycoprotein 1 induces endothelial tubulogenesis through interaction with cell surface proteoglycans
Categories:
Serum-free and Speciality Media 
Authors:
Lisboa FA, Warren J, Sulkowski G, Aparicio M, David G, Zudaire E, Dveksler GS 
In:
J Biol Chem (2011) 286(9): 7577-86 
Optimization of the PiggyBac transposon system for the sustained genetic modification of human T lymphocytes
Categories:
Transfection 
Authors:
Nakazawa Y, Huye LE, Dotti G, Foster AE, Vera JF, Manuri PR, June CH, Rooney CM, Wilson MH. 
In:
J Immunother (2009) 32(8): 826-36 
AKT inhibitor, GSK690693, induces growth inhibition and apoptosis in acute lymphoblastic leukemia cell lines
Categories:
 
Authors:
Levy DS, Kahana JA, Kumar R. 
In:
Blood (2009) 113(8): 1723-9 
Modulation of T cell activation by stomatin-like protein 2
Categories:
Transfection 
Authors:
Kirchhof MG, Chau LA, Lemke CD, Vardhana S, Darlington PJ, MÜ¡rquez ME, Taylor R, Rizkalla K, Blanca I, Dustin ML, Madrenas J 
In:
J Immunol (2008) 181(3): 1927-36 
ICOS ligation recruits the p50alpha PI3K regulatory subunit to the immunological synapse
Categories:
Transfection 
Authors:
Fos C, Salles A, Lang V, Carrette F, Audebert S, Pastor S, Ghiotto M, Olive D, Bismuth G, Nunes JA 
In:
J Immunol (2008) 181(3): 1969-77 
VSV oncolysis of T lymphocytes requires cell cycle entry and translation initiation
Categories:
Transfection 
Authors:
Oliere S, Arguello M, Mesplede T, Tumilasci V, Nakhaei P, Stojdl D, Sonenberg N, Bell J, Hiscott J 
In:
J Virol (2008) 82(12): 5735-49 
Orphan nuclear receptor NR4A2 expressed in T cells from multiple sclerosis mediates production of inflammatory cytokines
Categories:
Transfection 
Authors:
Doi Y, Oki S, Ozawa T, Hohjoh H, Miyake S, Yamamura T 
In:
Proc Natl Acad Sci USA (2008) 105(24): 8381-6 
SUMOylation Regulates the Transcriptional Activity of JunB in T Lymphocytes
Categories:
Transfection 
Authors:
Garaude J, Farras R, Bossis G, Charni S, Piechaczyk M, Hipskind RA, Villalba M 
In:
J Immunol (2008) 180(9): 5983-90 
Sleeping Beauty Transposon-mediated Engineering of Human Primary T Cells for Therapy of CD19(+) Lymphoid Malignancies
Categories:
Transfection 
Authors:
Huang X, Guo H, Kang J, Choi S, Zhou TC, Tammana S, Lees CJ, Li ZZ, Milone M, Levine BL, Tolar J, June CH, Scott McIvor R, Wagner JE, Blazar BR, Zhou X 
In:
Mol Ther (2008) 16(3): 580-9 
Transcription factor FOXO3a controls the persistence of memory CD4(+) T cells during HIV infection
Categories:
Transfection 
Authors:
van Grevenynghe J, Procopio FA, He Z, Chomont N, Riou C, Zhang Y, Gimmig S, Boucher G, Wilkinson P, Shi Y, Yassine-Diab B, Said EA, Trautmann L, El Far M, Balderas RS, Boulassel MR, Routy JP, Haddad EK, Sekaly RP 
In:
Nat Med (2008) 14(3): 266-74 
Orai1 and STIM1 move to the immunological synapse and are up-regulated during T cell activation
Categories:
Transfection 
Authors:
Lioudyno MI, Kozak JA, Penna A, Safrina O, Zhang SL, Sen D, Roos J, Stauderman KA, Cahalan MD 
In:
Proc Natl Acad Sci USA (2008) 105(6): 2011-6 
Differential contribution of NFATc2 and NFATc1 to TNF-alpha gene expression in T cells
Categories:
Transfection 
Authors:
Kaminuma O, Kitamura F, Kitamura N, Hiroi T, Miyoshi H, Miyawaki A, Miyatake S 
In:
J Immunol (2008) 180(1): 319-26 
4-1BBL Induces TNF Receptor-Associated Factor 1-Dependent Bim Modulation in Human T Cells and Is a Critical Component in the Costimulation-Dependent Rescue of Functionally Impaired HIV-Specific CD8 T Cells
Categories:
Transfection 
Authors:
Wang C, Wen T, Routy JP, Bernard NF, Sekaly RP, Watts TH 
In:
J Immunol (2007) 179(12): 8252-63 
Cutting Edge: Atypical PKCs Regulate T Lymphocyte Polarity and Scanning Behavior
Categories:
Transfection 
Authors:
Real E, Faure S, Donnadieu E, Delon J 
In:
J Immunol (2007) 179(9): 5649-52 
Induction of FOXP3 expression in naive human CD4+FOXP3 T cells by T-cell receptor stimulation is transforming growth factor-beta dependent but does not confer a regulatory phenotype
Categories:
Transfection 
Authors:
Tran DQ, Ramsey H, Shevach EM 
In:
Blood (2007) 110(8): 2983-90 
Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef
Categories:
Transfection 
Authors:
Laforge M, Petit F, Estaquier J, Senik A 
In:
J Virol (2007) 81(20): 11426-40 
Elf-1 Binds to GGAA Elements on the FcR Promoter and Represses Its Expression
Categories:
Transfection 
Authors:
Juang YT, Sumibcay L, Tolnay M, Wang Y, Kyttaris VC, Tsokos GC 
In:
J Immunol (2007) 179(7): 4884-9 
Inhibition of T cell activation by cyclic adenosine 5'-monophosphate requires lipid raft targeting of protein kinase a type I by the a-kinase anchoring protein ezrin
Categories:
Transfection 
Authors:
Ruppelt A, Mosenden R, Grönholm M, Aandahl EM, Tobin D, Carlson CR, Abrahamsen H, Herberg FW, Carpén O, Taskén K 
In:
J Immunol (2007) 179(8): 5159-68 
Impaired T Cell Protein Kinase C{delta} Activation Decreases ERK Pathway Signaling in Idiopathic and Hydralazine-Induced Lupus
Categories:
Transfection 
Authors:
Gorelik G, Fang JY, Wu A, Sawalha AH, Richardson B 
In:
J Immunol (2007) 179(8): 5553-63 
CXCL12-induced partitioning of flotillin-1 with lipid rafts plays a role in CXCR4 function
Categories:
Transfection 
Authors:
Giri B, Dixit VD, Ghosh MC, Collins GD, Khan IU, Madara K, Weeraratna AT, Taub DD 
In:
Eur J Immunol (2007) 37(8): 2104-16 
HIV-1 Protease Cleaves Procaspase 8 In Vivo
Categories:
Transfection 
Authors:
Nie Z, Bren GD, Vlahakis SR, Schimnich AA, Brenchley JM, Trushin SA, Warren S, Schnepple DJ, Kovacs CM, Loutfy MR, Douek DC, Badley AD 
In:
J Virol (2007) 81(13): 6947-56 
Apoptosis-inducing factor regulates death in peripheral T cells
Categories:
Transfection 
Authors:
Srivastava S, Banerjee H, Chaudhry A, Khare A, Sarin A, George A, Bal V, Durdik JM, Rath S 
In:
J Immunol (2007) 179(2): 797-803 
Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression
Categories:
Transfection 
Authors:
Bopp T, Becker C, Klein M, Klein-Heßling S, Palmetshofer A, Serfling E, Heib V, Becker M, Kubach J, Schmitt S, Stoll S, Schild H, Staege MS, Stassen M, Jonuleit H, Schmitt E 
In:
J Exp Med (2007) 204(6): 1303-1310 
ADAM10 regulates FasL cell surface expression and modulates FasL-induced cytotoxicity and activation-induced cell death
Categories:
Transfection 
Authors:
Schulte M, Reiss K, Lettau M, Maretzky T, Ludwig A, Hartmann D, de Strooper B, Janssen O, Saftig P 
In:
Cell Death Differ (2007) 14(5): 1040-9 
Production of Specific mRNA Transcripts, Usage of an Alternate Promoter, and Octamer-Binding Transcription Factors Influence the Surface Expression Levels of the HIV Coreceptor CCR5 on Primary T Cells
Categories:
Transfection 
Authors:
Mummidi S, Adams LM, Vancompernolle SE, Kalkonde M, Camargo JF, Kulkarni H, Bellinger AS, Bonello G, Tagoh H, Ahuja SS, Unutmaz D, Ahuja SK 
In:
J Immunol (2007) 178(9): 5668-81 
Epstein-Barr Virus (EBV) Latent Membrane Protein-1 Down-Regulates Tumor Necrosis Factor- (TNF-) Receptor-1 and Confers Resistance to TNF--Induced Apoptosis in T Cells: Implication for the Progression to T-Cell Lymphoma in EBV-Associated Hemophagocytic Syndrome
Categories:
Transfection 
Authors:
Chuang HC, Lay JD, Chuang SE, Hsieh WC, Chang Y, Su IJ 
In:
Am J Pathol (2007) 170(5): 1607-17 
Activation signal transduction by beta1 integrin in T cells from patients with systemic lupus erythematosus
Categories:
Transfection 
Authors:
Nakayamada S, Saito K, Nakano K, Tanaka Y 
In:
Arthritis Rheum (2007) 56(5): 1559-1568 
NRAS mutation causes a human autoimmune lymphoproliferative syndrome
Categories:
Transfection 
Authors:
Oliveira JB, Bidère N, Niemela JE, Zheng L, Sakai K, Nix CP, Danner RL, Barb J, Munson PJ, Puck JM, Dale J, Straus SE, Fleisher TA, Lenardo MJ 
In:
Proc Natl Acad Sci USA (2007) 104(21): 8953-8958 
c-Myb contributes to G2/M cell cycle transition in human hematopoietic cells by direct regulation of cyclin B1 expression
Categories:
Transfection 
Authors:
Nakata Y, Shetzline S, Sakashita C, Kalota A, Rallapalli R, Rudnick SI, Zhang Y, Emerson SG, Gewirtz AM 
In:
Mol Cell Biol (2007) 27(6): 2048-58 
Wnt signaling induces matrix metalloproteinase expression and regulates T cell transmigration
Categories:
Transfection 
Authors:
Wu B, Crampton SP, Hughes CC 
In:
Immunity (2007) 26(2): 227-39 
Induction of inducible NO synthase in bystander human T cells increases allogeneic responses in the vasculature
Categories:
Transfection 
Authors:
Choy JC, Wang Y, Tellides G, Pober JS 
In:
Proc Natl Acad Sci USA (2007) 104(4): 1313-8 
Talin1 Regulates TCR-Mediated LFA-1 Function
Categories:
Transfection 
Authors:
Simonson WT, Franco SJ, Huttenlocher A 
In:
J Immunol (2006) 177(11): 7707-14 
Cutting Edge: TCR-induced NAB2 enhances T cell function by coactivating IL-2 transcription
Categories:
Transfection 
Authors:
Collins S, Wolfraim LA, Drake CG, Horton MR, Powell JD 
In:
J Immunol (2006) 177(12): 8301-5 
Orchestration of lymphocyte chemotaxis by mitochondrial dynamics
Categories:
Transfection 
Authors:
Campello S, Lacalle RA, Bettella M, Manes S, Scorrano L, Viola A 
In:
J Exp Med (2006) 203(13): 2879-86 
Histidine Phosphorylation of the Potassium Channel KCa3.1 by Nucleoside Diphosphate Kinase B Is Required for Activation of KCa3.1 and CD4 T Cells
Categories:
Transfection 
Authors:
Srivastava S, Li Z, Ko K, Choudhury P, Albaqumi M, Johnson AK, Yan Y, Backer JM, Unutmaz D, Coetzee WA, Skolnik EY 
In:
Mol Cell (2006) 24(5): 665-75 
Measles virus induces expression of SIP110, a constitutively membrane clustered lipid phosphatase, which inhibits T cell proliferation
Categories:
Transfection 
Authors:
Avota E, Harms H, Schneider-Schaulies S 
In:
Cell Microbiol (2006) 8(11): 1826-39 
Expression of Nef Downregulates CXCR4, the Major Coreceptor of Human Immunodeficiency Virus, From the Surface of Target Cells and Thereby Enhances Resistance to Superinfection
Categories:
Transfection 
Authors:
Venzke S, Michel N, Allespach I, Fackler OT, Keppler OT 
In:
J Virol (2006) 80(22): 11141-52 
Human immunodeficiency virus type 1 vpr induces DNA replication stress in vitro and in vivo
Categories:
Transfection 
Authors:
Zimmerman ES, Sherman MP, Blackett JL, Neidleman JA, Kreis C, Mundt P, Williams SA, Warmerdam M, Kahn J, Hecht FM, Grant RM, de Noronha CM, Weyrich AS, Greene WC, Planelles V 
In:
J Virol (2006) 80(21): 10407-18 
CD28 interaction with filamin-A controls lipid raft accumulation at the T-cell immunological synapse
Categories:
Transfection 
Authors:
Tavano R, Contento RL, Baranda SJ, Soligo M, Tuosto L, Manes S, Viola A 
In:
Nat Cell Biol (2006) 8(11): 1270-6 
T Cell Receptor-Interacting Molecule Acts as a Chaperone to Modulate Surface Expression of the CTLA-4 Coreceptor
Categories:
Transfection 
Authors:
Valk E, Leung R, Kang H, Kaneko K, Rudd CE, Schneider H 
In:
Immunity (2006) 25(5): 807-821 
TGF-beta1 in SP-A preparations influences immune suppressive properties of SP-A on human CD4+ T lymphocytes
Categories:
Transfection 
Authors:
Kunzmann S, Wright JR, Steinhilber W, Kramer BW, Blaser K, Speer CP, Schmidt-Weber C 
In:
Am J Physiol Lung Cell Mol Physiol (2006) 291(4): L747-56 
Glucocorticoids cause rapid dissociation of a T-cell-receptor-associated protein complex containing LCK and FYN
Categories:
Transfection 
Authors:
Lowenberg M, Verhaar AP, Bilderbeek J, Marle JV, Buttgereit F, Peppelenbosch MP, van Deventer SJ, Hommes DW 
In:
EMBO Rep (2006) 7(10): 1023-9 
DOCK2 is required for chemokine-promoted human T lymphocyte adhesion under shear stress mediated by the integrin alpha4beta1
Categories:
Transfection 
Authors:
Garcia-Bernal D, Sotillo-Mallo E, Nombela-Arrieta C, Samaniego R, Fukui Y, Stein JV, Teixido J 
In:
J Immunol (2006) 177(8): 5215-25 
Splice variants of human FOXP3 are functional inhibitors of human CD4 T-cell activation
Categories:
Transfection 
Authors:
Smith EL, Finney HM, Nesbitt AM, Ramsdell F, Robinson MK 
In:
Immunology (2006) 119(2): 203-11 
T cell receptor for antigen induces linker for activation of T cell-dependent activation of a negative signaling complex involving Dok-2, SHIP-1, and Grb-2
Categories:
Transfection 
Authors:
Dong S, Corre B, Foulon E, Dufour E, Veillette A, Acuto O, Michel F 
In:
J Exp Med (2006) 203(11): 2509-18 
CTLA-4IG suppresses reactive oxygen species by preventing synovial adherent cell-induced inactivation of Rap1, a Ras family GTPASE mediator of oxidative stress in rheumatoid arthritis T cells
Categories:
Transfection 
Authors:
Remans PH, Wijbrandts CA, Sanders ME, Toes RE, Breedveld FC, Tak PP, van Laar JM, Reedquist KA 
In:
Arthritis Rheum (2006) 54(10): 3135-43 
Human T-Cell Leukemia Virus Type 1 (HTLV-1) and HTLV-2 Use Different Receptor Complexes To Enter T Cells
Categories:
Transfection 
Authors:
Jones KS, Fugo K, Petrow-Sadowski C, Huang Y, Bertolette DC, Lisinski I, Cushman SW, Jacobson S, Ruscetti FW 
In:
J Virol (2006) 80(17): 8291-302 
Role of DNA methylation for expression of novel stem cell marker CDCP1 in hematopoietic cells
Categories:
Transfection 
Authors:
Kimura H, Morii E, Ikeda JI, Ezoe S, Xu JX, Nakamichi N, Tomita Y, Shibayama H, Kanakura Y, Aozasa K 
In:
Leukemia (2006) 20(9): 1551-6 
Proprotein convertase furin is preferentially expressed in T helper 1 cells and regulates interferon-
Categories:
Transfection 
Authors:
Pesu M, Muul L, Kanno Y, O'shea JJ 
In:
Blood (2006) 108(3): 983-5 
Lymphocyte Chemotaxis Is Regulated by Histone Deacetylase 6, Independently of Its Deacetylase Activity
Categories:
Transfection 
Authors:
Cabrero JR, Serrador JM, Barreiro O, Mittelbrunn M, Naranjo-Suarez S, Martin-Cofreces N, Vicente-Manzanares M, Mazitschek R, Bradner JE, Avila J, Valenzuela-Fernandez A, Sanchez-Madrid F 
In:
Mol Biol Cell (2006) 17(8): 3435-3445 
Filamin A is required for T cell activation mediated by protein kinase C-theta
Categories:
Transfection 
Authors:
Hayashi K, Altman A 
In:
J Immunol (2006) 177(3): 1721-8 
Autophagy is involved in T cell death after binding of HIV-1 envelope proteins to CXCR4
Categories:
Transfection 
Authors:
Espert L, Denizot M, Grimaldi M, Robert-Hebmann V, Gay B, Varbanov M, Codogno P, Biard-Piechaczyk M 
In:
J Clin Invest (2006) 116(8): 2161-72 
A2A adenosine receptor protects tumors from antitumor T cells
Categories:
Transfection 
Authors:
Ohta A, Gorelik E, Prasad SJ, Ronchese F, Lukashev D, Wong MK, Huang X, Caldwell S, Liu K, Smith P, Chen JF, Jackson EK, Apasov S, Abrams S, Sitkovsky M 
In:
Proc Natl Acad Sci USA (2006) 103(35): 13132-7 
Small Interfering RNA-Mediated Knockdown of Notch Ligands in Primary CD4+ T Cells and Dendritic Cells Enhances Cytokine Production
Categories:
Transfection 
Authors:
Stallwood Y, Briend E, Ray KM, Ward GA, Smith BJ, Nye E, Champion BR, McKenzie GJ 
In:
J Immunol (2006) 177(2): 885-95 
Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7
Categories:
Transfection 
Authors:
Granum S, Sundvold-Gjerstad V, Dai KZ, Kolltveit KM, Hildebrand K, Huitfeldt HS, Lea T, Spurkland A 
In:
BMC Immunol (2006) 7: 15 
Dysregulated T cell expression of TIM3 in multiple sclerosis
Categories:
Transfection 
Authors:
Koguchi K, Anderson DE, Yang L, O'connor KC, Kuchroo VK, Hafler DA 
In:
J Exp Med (2006) 203(6): 1413-8 
Protein Kinase C (PKC) and PKC Are the Major PKC Isotypes Involved in TCR Down-Regulation
Categories:
Transfection 
Authors:
von Essen M, Nielsen MW, Bonefeld CM, Boding L, Larsen JM, Leitges M, Baier G, Odum N, Geisler C 
In:
J Immunol (2006) 176(12): 7502-10 
Loss of Siglec expression on T lymphocytes during human evolution
Categories:
Transfection 
Authors:
Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A 
In:
Proc Natl Acad Sci USA (2006) 103(20): 7765-70 
The adaptor protein Nck interacts with Fas ligand: Guiding the death factor to the cytotoxic immunological synapse
Categories:
Transfection 
Authors:
Lettau M, Qian J, Linkermann A, Latreille M, Larose L, Kabelitz D, Janssen O 
In:
Proc Natl Acad Sci USA (2006) 103(15): 5911-6 
Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development
Categories:
Transfection 
Authors:
Gavin MA, Torgerson TR, Houston E, Deroos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY 
In:
Proc Natl Acad Sci USA (2006) 103(17): 6659-64 
Foxp3 represses retroviral transcription by targeting both NF-kappaB and CREB pathways
Categories:
Transfection 
Authors:
Grant C, Oh U, Fugo K, Takenouchi N, Griffith C, Yao K, Newhook TE, Ratner L, Jacobson S 
In:
PLoS Pathog (2006) 2(4): e33 
Modulation of specific surface receptors and activation sensitization in primary resting CD4+ T lymphocytes by the Nef protein of HIV-1
Categories:
Transfection 
Authors:
Keppler OT, Tibroni N, Venzke S, Rauch S and Fackler OT 
In:
J Leukoc Biol (2006) 79(3): 616-27 
Molecular Mechanisms Underlying FOXP3 Induction in Human T Cells
Categories:
Transfection 
Authors:
Mantel PY, Ouaked N, Ruckert B, Karagiannidis C, Welz R, Blaser K, Schmidt-Weber CB 
In:
J Immunol (2006) 176(6): 3593-602 
Lack of Tyrosine 320 Impairs Spontaneous Endocytosis and Enhances Release of HLA-B27 Molecules
Categories:
Transfection 
Authors:
Santos SG, Antoniou AN, Sampaio P, Powis SJ, Arosa FA 
In:
J Immunol (2006) 176(5): 2942-9 
The HIV-1 Vpr and glucocorticoid receptor complex is a gain-of-function interaction that prevents the nuclear localization of PARP-1
Categories:
Transfection 
Authors:
Muthumani K, Choo AY, Zong WX, Madesh M, Hwang DS, Premkumar A, Thieu KP, Emmanuel J, Kumar S, Thompson CB, Weiner DB 
In:
Nat Cell Biol (2006) 8(2): 170-9 
Suppression of MIP-1beta transcription in human T cells is regulated by inducible cAMP early repressor (ICER)
Categories:
Transfection 
Authors:
Barabitskaja O, Foulke JS Jr, Pati S, Bodor J, Reitz MS Jr 
In:
J Leukoc Biol (2006) 79(2): 378-87 
Cellular Notch responsiveness is defined by phosphoinositide 3-kinase-dependent signals
Categories:
Transfection 
Authors:
McKenzie G, Ward G, Stallwood Y, Briend E, Papadia S, Lennard A, Turner M, Champion B, Hardingham GE 
In:
BMC Cell Biol (2006) 7: 10 
Azathioprine Suppresses Ezrin-Radixin-Moesin-Dependent T Cell-APC Conjugation through Inhibition of Vav Guanosine Exchange Activity on Rac Proteins
Categories:
Transfection 
Authors:
Poppe D, Tiede I, Fritz G, Becker C, Bartsch B, Wirtz S, Strand D, Tanaka S, Galle PR, Bustelo XR and Neurath MF 
In:
J Immunol (2006) 176(1): 640-651 
cIAP2 is a ubiquitin protein ligase for BCL10 and is dysregulated in mucosa-associated lymphoid tissue lymphomas
Categories:
Transfection 
Authors:
Hu S, Du MQ, Park SM, Alcivar A, Qu L, Gupta S, Tang J, Baens M, Ye H, Lee TH, Marynen P, Riley JL and Yang X 
In:
J Clin Invest (2006) 116(1): 174-181 
A role for the neuronal protein collapsin response mediator protein 2 in T lymphocyte polarization and migration
Categories:
Transfection 
Authors:
Vincent P, Collette Y, Marignier R, Vuaillat C, Rogemond V, Davoust N, Malcus C, Cavagna S, Gessain A, Machuca-Gayet I, Belin MF, Quach T and Giraudon P 
In:
J Immunol (2005) 175(11): 7650-7660 
Activation or suppression of NFkappaB by HPK1 determines sensitivity to activation-induced cell death
Categories:
Transfection 
Authors:
Brenner D, Golks A, Kiefer F, Krammer PH and Arnold R 
In:
EMBO J (2005) 24(24): 4279-4290 
The transcription factor GATA-1 potently represses the expression of the HIV-1 co-receptor CCR5 in human T cells and dendritic cells
Categories:
Transfection 
Authors:
Sundrud MS, Vancompernolle SE, Eger KA, Bruno TC, Subramaniam A, Mummidi S, Ahuja SK and Unutmaz D 
In:
Blood (2005) 106(10): 3440-3448 
Sphingosine Kinase 1 Is a Negative Regulator of CD4+ Th1 Cells
Categories:
Transfection 
Authors:
Yang J, Castle BE, Hanidu A, Stevens L, Yu Y, Li X, Stearns C, Papov V, Rajotte D and Li J 
In:
J Immunol (2005) 175(10): 6580-6588 
Determination of Cellularly Processed HLA-A2402-Restricted Novel CTL Epitopes Derived from Two Cancer Germ Line Genes, MAGE-A4 and SAGE
Categories:
Transfection 
Authors:
Miyahara Y, Naota H, Wang L, Hiasa A, Goto M, Watanabe M, Kitano S, Okumura S, Takemitsu T, Yuta A, Majima Y, Lemonnier FA, Boon T and Shiku H 
In:
Clin Cancer Res (2005) 11(15): 5581-5589 
Nucleofection of non-B cells with mini-Epstein-Barr virus DNA
Categories:
Transfection 
Authors:
Radons J, Gross C, Stangl S and Multhoff G 
In:
J Immunol Methods (2005) 303(1-2): 135-141 
Vav1 and Rac Control Chemokine-promoted T Lymphocyte Adhesion Mediated by the Integrin 41
Categories:
Transfection 
Authors:
Garcia-Bernal D, Wright N, Sotillo-Mallo E, Nombela-Arrieta C, Stein JV, Bustelo XR and Teixido J 
In:
Mol Biol Cell (2005) 16(7): 3223-3235 
TGF- signaling of human T cells is modulated by the ancillary TGF- receptor endoglin
Categories:
Transfection 
Authors:
Schmidt-Weber CB, Letarte M, Kunzmann S, Ruckert B, Bernabeu C and Blaser K 
In:
Int Immunol (2005) 17(7): 921-930 
Defective Vav expression and impaired F-actin reorganization in a subset of patients with common variable immunodeficiency characterized by T-cell defects
Categories:
Transfection 
Authors:
Paccani SR, Boncristiano M, Patrussi L, Ulivieri C, Wack A, Valensin S, Hirst TR, Amedei A, Del Prete G, Telford JL, D'Elios MM and Baldari CT 
In:
Blood (2005) 106(2): 626-634 
Highly efficient endogenous human gene correction using designed zinc-finger nucleases
Categories:
Transfection 
Authors:
Urnov FD, Miller JC, Lee YL, Beausejour CM, Rock JM, Augustus S, Jamieson AC, Porteus MH, Gregory PD and Holmes MC 
In:
Nature (2005) 435(7042): 646-651 
Regulation of lck degradation and refractory state in CD8+ cytotoxic T lymphocytes
Categories:
Transfection 
Authors:
Uhlin M, Masucci MG and Levitsky V 
In:
Proc Natl Acad Sci USA (2005) 102(26): 9264-9269 
Lymphocyte arrest requires instantaneous induction of an extended LFA-1 conformation mediated by endothelium-bound chemokines
Categories:
Transfection 
Authors:
Shamri R, Grabovsky V, Gauguet JM, Feigelson S, Manevich E, Kolanus W, Robinson MK, Staunton DE, von Andrian UH and Alon R 
In:
Nat Immunol (2005) 6(5): 497-506 
A novel E3 ubiquitin ligase TRAC-1 positively regulates T cell activation
Categories:
Transfection 
Authors:
Zhao H, Li CC, Pardo J, Chu PC, Liao CX, Huang J, Dong JG, Zhou X, Huang Q, Huang B, Bennett MK, Molineaux SM, Lu H, Daniel-Issakani S, Payan DG and Masuda ES 
In:
J Immunol (2005) 174(9): 5288-5297 
Virus-induced dysfunction of CD4(+)CD25(+) T cells in patients with HTLV-I-associated neuroimmunological disease
Categories:
Transfection 
Authors:
Yamano Y, Takenouchi N, Li HC, Tomaru U, Yao K, Grant CW, Maric DA and Jacobson S 
In:
J Clin Invest (2005) 115(5): 1361-1368 
Role of GATA-3 in IL-5 gene transcription by CD4+ T cells of asthmatic patients
Categories:
Transfection 
Authors:
Kaminuma O, Mori A, Kitamura N, Hashimoto T, Kitamura F, Inokuma S, Miyatake S 
In:
Int Arch Allergy Immunol (2005) 137 Suppl 1: 55-9 
Systemic lupus erythematosus serum IgG increases CREM binding to the IL-2 promoter and suppresses IL-2 production through CaMKIV
Categories:
Transfection 
Authors:
Juang YT, Wang Y, Solomou EE, Li Y, Mawrin C, Tenbrock K, Kyttaris VC and Tsokos GC 
In:
J Clin Invest (2005) 115(4): 996-1005 
Rodent cells support key functions of the human immunodeficiency virus type 1 pathogenicity factor Nef.
Categories:
Transfection 
Authors:
Keppler OT, Allespach I, Schuller L, Fenard D, Greene WC and Fackler OT 
In:
J Virol (2005) 79(3): 1655-1665 
Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton
Categories:
Transfection 
Authors:
Barda-Saad M, Braiman A, Titerence R, Bunnell SC, Barr VA and Samelson LE 
In:
Nat Immunol (2005) 6(1): 80-89 
Interleukin-12-induced IFN-gamma production by human peripheral blood T cells is regulated by mammalian target of rapamycin (mTOR)
Categories:
Transfection 
Authors:
Kusaba H, Ghosh P, Derin R, Buchholz M, Sasaki C, Madara K and Longo DL 
In:
J Biol Chem (2005) 280(2): 1037-1043 
Short interfering RNA (siRNA) targeting the Lyn kinase induces apoptosis in primary, and drug-resistant, BCR-ABL1(+) leukemia cells
Categories:
Transfection 
Authors:
Ptasznik A, Nakata Y, Kalota A, Emerson SG and Gewirtz AM 
In:
Nat Med (2004) 10(11): 1187-1189 
AILIM/ICOS signaling induces T-cell migration/polarization of memory/effector T-cells
Categories:
Transfection 
Authors:
Okamoto N, Nukada Y, Tezuka K, Ohashi K, Mizuno K and Tsuji T 
In:
Int Immunol (2004) 16(10): 1515-1522 
Human cytokine-induced killer cells have enhanced in vitro cytolytic activity via non-viral interleukin-2 gene transfer
Categories:
Transfection 
Authors:
Nagaraj S, Ziske C and Schmidt-Wolf IG 
In:
Genet Vaccines Ther (2004) 2(1): 12 
Ligand-independent redistribution of Fas (CD95) into lipid rafts mediates clonotypic T cell death
Categories:
Transfection 
Authors:
Muppidi JR and Siegel RM 
In:
Nat Immunol (2004) 5(2): 182-189 
Munc13-4 is essential for cytolytic granules fusion and is mutated in a form of familial hemophagocytic lymphohistiocytosis (FHL3)
Categories:
Transfection 
Authors:
Feldmann J, Callebaut I, Raposo G, Certain S, Bacq D, Dumont C, Lambert N, Ouachee-Chardin M, Chedeville G, Tamary H, Minard-Colin V, Vilmer E, Blanche S, Le Deist F, Fischer A and de Saint Basile G 
In:
Cell (2003) 115(4): 461-473 
Adoptive transfer of costimulated T cells induces lymphocytosis in patients with relapsed/refractory non-Hodgkin lymphoma following CD34+-selected hematopoietic cell transplantation.
Categories:
Serum-free and Speciality Media 
Authors:
Laport GG, Levine BL, Stadtmauer EA, Schuster SJ, Luger SM, Grupp S, Bunin N,  Strobl FJ, Cotte J, Zheng Z, Gregson B, Rivers P, Vonderheide RH, Liebowitz DN, Porter DL, June CH. 
In:
Blood (2003) 102 (6): 2004-13 
Functional CRISPR dissection of gene networks controlling human regulatory T cell identity
Categories:
Transfection 
Authors:
Schumann K, Raju SS, Lauber M, Kolb S, Shifrut E, Cortez JT, Skartsis N, Nguyen VQ, Woo JM, Roth TL, Yu R, Nguyen MLT, Simeonov DR, Nguyen DN, Targ S, Gate RE, Tang Q, Bluestone JA, Spitzer MH, Ye CJ, Marson A 
In:
Nat Immunol () 11: 1456-1466 
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