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Do you offer on-site maintencance services for the 4D-Nucleofector Core, X, Y or LV Unit? 

No, currently we can only offer in-house maintenance services, but we are working on a process for on-site services.

Do you have any instructions for performing a trypan blue stain?

Yes. Please click on the link under related literature.

 What are the main differences between Earle's BSS and Hank's BSS? 

A balanced salt solution (BSS) is a solution made to a physiological pH and salt concentration.  Solutions most commonly include sodium, potassium, calcium, magnesium, and chloride.   Balanced salt solutions are used for washing...

What is HEPES? 

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twelve Good's buffers. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH...

What is the purpose of EDTA in trypsin? 

EDTA is needed with trypsin because it helps bind up the divalent cations in the medium.  Like calcium and magnesium.  Helps to keep the cells from sticking to each other.  If its not there….you would need a higher concentration of...

I am using the acute leukemia HL-60 cell line for CAR-T cells experiments. Do you have a transfection protocol for genome editing?

The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors).Transfection protocols can be found...

Do you have any tips for the culture of primary cells or cell line prior to transfection?

Yes. Please see our Techincal Reference Guide which contains many helpful tips.

Do your ProCHO Media contain hypoxanthine and thymidine?

All of our ProCHO™ media, does not contain Thymidine and Hypoxanthine. So if the customer has a CHO Clone which is dhfr-negativ, he will need to add Thymidine and Hypoxanthine or otherwise the cells will not grow. If the customer has a CHO clone...

What are Silensomes HLM?

Silensomes™ HLM are pooled human liver microsomes (HLM) chemically and irreversibly inactivated for one specific cytochrome P450 (CYP450) activity (1A2, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4) using a mechanism based inhibitor (MBI). Silensomes™ HLM...

What can Silensomes HLM be used for?

Silensomes™ HLM can be used in cytochrome P450 screening and phenotyping assays, to identify enzymes responsible for the metabolism of drug candidates. Silensomes™ HLM can be used to predict directly the in-vitro fm for a particular CYP450 enzyme (fm...