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Cell Information
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FAQ
(675)
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675 results sorted by
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Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells. The MycoZap™ Reagent eliminates...
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The MycoAlert® Assay Control Set (LT07-518) is available separately and includes a lyophilized positive control (1 ml) and assay buffer (2 ml) for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls...
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We have compared the ViaLight kits to all other assays routinely used in cell viability studies and have found the performance to be superior in every case. This is due to the combination of extreme sensitivity and wide dynamic range of...
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The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95% of...
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No. The MycoAlert® Assay is sold for Research Use Only.
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The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.
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Certain compounds will quench the light emission in bioluminescent reactions. Typically these are strong red dyes (however, the ViaLight assays have been designed to function in all phenol red containing media commonly used in cell...
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6
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It should be noted that in order to maintain the system with two pump heads these will need to operate at the same rate, as otherwise the pumps will either empty of flood the chamber. Setting up the system with a pump at the outlet may make...
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8
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°
C
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No, cryopreserved cells should be stored in liquid Nitrogen. Storing the cells at lower temperatures can irreversibly damage the cells.
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Lonza's QC uses HCM (CC-3198) supplemented with 20% FBS and 25 mM Hepes. No plating media is necessary when seeding for spheroids.
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