Data Type
Cell Information
(858)
Citations
(4969)
FAQ
(515)
Culture Media
(61)
Category
Primary Cells and Media
(200)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin and Pyrogen Testing
(54)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(135)
Cancer Research/Cell Biology
(77)
Immunotherapy / Hematology
(63)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(22)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(14)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
515 results sorted by
relevance
alphabetical
newest first
oldest first
W
h
a
t
i
s
t
h
e
p
r
o
o
f
t
h
a
t
D
N
A
e
n
t
e
r
s
t
h
e
n
u
c
l
e
u
s
d
u
r
i
n
g
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
There are two facts that prove that the DNA is directly transported into the nucleus: 1.) Using the Nucleofector® Technology, transfection of non-dividing cells is possible. With conventional non-viral transfection methods cell division is a...
H
o
w
c
a
n
I
r
e
m
o
v
e
t
h
e
c
e
l
l
s
f
r
o
m
t
h
e
1
0
0
u
L
N
u
c
l
e
o
c
u
v
e
t
t
e
®
V
e
s
s
e
l
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
I
s
t
h
e
r
e
a
n
y
a
l
t
e
r
n
a
t
i
v
e
?
We recommend using the plastic single use pipettes provided in the kits. These pipettes prevent any damage to the cells and ensure a good cell recovery.
W
h
a
t
i
s
t
h
e
b
a
s
i
c
p
r
i
n
c
i
p
l
e
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
?
The Nucleofector® Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell-type...
I
s
y
o
u
r
p
m
a
x
G
F
P
c
o
n
t
r
o
l
v
e
c
t
o
r
s
u
p
p
l
i
e
d
w
i
t
h
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
K
i
t
s
s
u
i
t
a
b
l
e
f
o
r
s
t
a
b
l
e
e
x
p
r
e
s
s
i
o
n
?
No. This vector is only supplied in our Nucleofector® Kits as a positive control and cannot be used for selection because it does not contain a mammalian selection marker. The only resistance gene expressed by this vector is kanamycin which is...
W
h
y
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
T
e
c
h
n
o
l
o
g
y
i
d
e
a
l
f
o
r
p
r
i
m
a
r
y
c
e
l
l
s
a
n
d
d
i
f
f
i
c
u
l
t
-
t
o
-
t
r
a
n
s
f
e
c
t
c
e
l
l
l
i
n
e
s
?
There are several reasons to choose the Nucleofector® Technology for your gene transfer experiments. One is the direct transfer of DNA to the cell nucleus. This makes gene expression independent from cell division. Therefore, the technique is the...
D
o
y
o
u
h
a
v
e
a
n
y
r
e
c
o
m
m
e
n
d
e
d
m
e
t
h
o
d
s
f
o
r
d
e
t
a
c
h
i
n
g
h
u
m
a
n
m
o
n
o
c
y
t
e
s
f
r
o
m
t
h
e
p
l
a
t
e
f
o
r
a
s
s
a
y
i
n
g
o
r
b
e
f
o
r
e
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
We have had good results by incubating the cells in ice cold PBS for 10 minutes and then rinsing the plates. Alternatively, the cells can be detached without a medium change by gently pipetting the cell suspension up and down. Our experience has also...
H
o
w
d
o
y
o
u
r
e
c
o
m
m
e
n
d
t
h
a
t
w
e
i
s
o
l
a
t
e
s
p
l
e
n
i
c
l
y
m
p
h
o
c
y
t
e
s
f
r
o
m
m
o
u
s
e
s
p
l
e
e
n
s
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
I
s
e
r
y
t
h
r
o
c
y
t
e
l
y
s
i
s
r
e
q
u
i
r
e
d
?
For the preparation of mouse spleen cells we recommend cutting the spleen once and passing the tissue through a 100µM cell strainer or steel mesh using a plunger. The cells are flushed into a petri dish containing PBS. In order to remove fat, cell...
D
o
y
o
u
r
e
c
o
m
m
e
n
d
p
o
s
i
t
i
v
e
s
e
l
e
c
t
i
o
n
o
r
d
e
p
l
e
t
i
o
n
f
o
r
t
h
e
p
u
r
i
f
i
c
a
t
i
o
n
o
f
H
u
m
a
n
m
o
n
o
c
y
t
e
s
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
We only recommend the depletion method. For Example, the RosetteSep™ Isolation Kit for human monocytes [Stem Cell Technologies, Cat. No 15028]. The advantage of depletion is that the monocytes are left untouched by antibodies during the process.
C
a
n
c
a
l
c
i
u
m
i
n
f
l
u
x
i
s
s
u
e
s
a
f
f
e
c
t
v
i
a
b
i
l
i
t
y
o
r
d
i
f
f
e
r
e
n
t
i
a
t
i
o
n
i
n
S
t
e
m
C
e
l
l
s
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
When cells are transfected by Nucleofection®, transient pores are generated in the cell membrane. Generally, these pores disappear within 15 minutes of transfection, but if cells are plated immediately after Nucleofection® in medias that contain...
D
o
e
s
i
t
m
a
t
t
e
r
i
f
t
h
e
P
B
S
u
s
e
d
f
o
r
m
o
n
o
c
y
t
e
e
n
r
i
c
h
m
e
n
t
c
o
n
t
a
i
n
s
c
a
l
c
i
u
m
a
n
d
m
a
g
n
e
s
i
u
m
,
i
f
t
h
e
c
e
l
l
s
s
h
o
u
l
d
b
e
u
s
e
d
l
a
t
e
r
i
n
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
The PBS should be calcium and magnesium free to prevent clumping of cells. We routinely use PBS without calcium and magnesium for example Lonza Cat. No. BEBP17-516Q
PREVIOUS
PAGE
1
PAGE
2
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
NEXT