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How can cells be cured of mycoplasma contamination?

Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells. The MycoZap™ Reagent eliminates...

Are there positive controls for the MycoAlert® Assay?

The MycoAlert® Assay Control Set (LT07-518) is available separately and includes a lyophilized positive control (1 ml) and assay buffer (2 ml) for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls...

How does the performance of the ViaLight kits compare with other assays routinely used in cell proliferation/cytotoxicity assays?  

We have compared the ViaLight kits to all other assays routinely used in cell viability studies and have found the performance to be superior in every case.  This is due to the combination of extreme sensitivity and wide dynamic range of...

Does the MycoALert® Assay recognize the whole spectrum of mycoplasma?

The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95% of...

Is the MycoAlert® Assay validated for use in GMP situations?

No. The MycoAlert® Assay is sold for Research Use Only.

How much sample do you need for accurate results with MycoAlert® Assay? Do you need cells or just supernatant?

The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.

What factors interfere with the ViaLight assay?

Certain compounds will quench the light emission in bioluminescent reactions.  Typically these are strong red dyes (however, the ViaLight assays have been designed to function in all phenol red containing media commonly used in cell...

How do you keep the media at an appropriate level in the Quasiv Vivo QV600 system when using transwell inserts?

It should be noted that in order to maintain the system with two pump heads these will need to operate at the same rate, as otherwise the pumps will either empty of flood the chamber. Setting up the system with a pump at the outlet may make...

Can I store my cryopreserved ampule at 80°C?

No, cryopreserved cells should be stored in liquid Nitrogen. Storing the cells at lower temperatures can irreversibly damage the cells.

What media should be used for spheroid formation with hepatocytes?

Lonza's QC uses HCM (CC-3198) supplemented with 20% FBS and 25 mM Hepes. No plating media is necessary when seeding for spheroids.