Data Type
Cell Information
(849)
Citations
(4665)
FAQ
(684)
Culture Media
(93)
Category
Primary Cells and Media
(266)
Transfection
(178)
Laboratory Instrumentation
(68)
Electrophoreses and Analysis
(55)
Bioassay
(48)
3D Cell Culture
(42)
Live Cell Imaging
(34)
Mycoplasma Detection and Prevention
(29)
Endotoxin Detection
(27)
Serum-free and Speciality Media
(11)
Classical Media and Reagents
(10)
Cell Lines and Primary Cancer Cells
(7)
Uncategorized
(7)
+ Show All
Research Area
Uncategorized
(173)
Basic Research
(147)
Cancer Research/Cell Biology
(84)
Immunotherapy / Hematology
(67)
Stem Cells
(54)
Molecular Biology
(40)
Neurobiology
(38)
Respiratory Research
(31)
Toxicology
(29)
Endotoxin Testing
(27)
Cardiovascular
(26)
Regenerative medicine
(24)
Dermatology/Tissue Engineering
(21)
Gene Expression
(13)
Gastroenterology
(4)
Parasitology
(3)
+ Show All
684 results sorted by
relevance
alphabetical
newest first
oldest first
D
o
y
o
u
o
f
f
e
r
o
n
-
s
i
t
e
m
a
i
n
t
e
n
c
a
n
c
e
s
e
r
v
i
c
e
s
f
o
r
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
™
C
o
r
e
,
X
,
Y
o
r
L
V
U
n
i
t
?
No, currently we can only offer in-house maintenance services, but we are working on a process for on-site services.
D
o
y
o
u
h
a
v
e
a
n
y
i
n
s
t
r
u
c
t
i
o
n
s
f
o
r
p
e
r
f
o
r
m
i
n
g
a
t
r
y
p
a
n
b
l
u
e
s
t
a
i
n
?
Yes. Please click on the link under related literature.
W
h
a
t
a
r
e
t
h
e
m
a
i
n
d
i
f
f
e
r
e
n
c
e
s
b
e
t
w
e
e
n
E
a
r
l
e
'
s
B
S
S
a
n
d
H
a
n
k
'
s
B
S
S
?
A balanced salt solution (BSS) is a solution made to a physiological pH and salt concentration. Solutions most commonly include sodium, potassium, calcium, magnesium, and chloride. Balanced salt solutions are used for washing...
W
h
a
t
i
s
H
E
P
E
S
?
HEPES ( 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent ; one of the twelve Good's buffers . HEPES is widely used in cell culture , largely because it is better at maintaining...
I
a
m
u
s
i
n
g
t
h
e
a
c
u
t
e
l
e
u
k
e
m
i
a
H
L
-
6
0
c
e
l
l
l
i
n
e
f
o
r
C
A
R
-
T
c
e
l
l
s
e
x
p
e
r
i
m
e
n
t
s
.
D
o
y
o
u
h
a
v
e
a
t
r
a
n
s
f
e
c
t
i
o
n
p
r
o
t
o
c
o
l
f
o
r
g
e
n
o
m
e
e
d
i
t
i
n
g
?
The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors). Transfection protocols can be...
D
o
y
o
u
h
a
v
e
a
n
y
t
i
p
s
f
o
r
t
h
e
c
u
l
t
u
r
e
o
f
p
r
i
m
a
r
y
c
e
l
l
s
o
r
c
e
l
l
l
i
n
e
p
r
i
o
r
t
o
t
r
a
n
s
f
e
c
t
i
o
n
?
Yes. Please see our Techincal Reference Guide which contains many helpful tips.
H
o
w
d
o
y
o
u
r
e
c
o
m
m
e
n
d
t
h
a
t
w
e
i
s
o
l
a
t
e
s
p
l
e
n
i
c
l
y
m
p
h
o
c
y
t
e
s
f
r
o
m
m
o
u
s
e
s
p
l
e
e
n
s
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
I
s
e
r
y
t
h
r
o
c
y
t
e
l
y
s
i
s
r
e
q
u
i
r
e
d
?
For the preparation of mouse spleen cells we recommend cutting the spleen once and passing the tissue through a 100µM cell strainer or steel mesh using a plunger. The cells are flushed into a petri dish containing PBS. In order to remove fat, cell...
D
o
y
o
u
r
e
c
o
m
m
e
n
d
p
o
s
i
t
i
v
e
s
e
l
e
c
t
i
o
n
o
r
d
e
p
l
e
t
i
o
n
f
o
r
t
h
e
p
u
r
i
f
i
c
a
t
i
o
n
o
f
H
u
m
a
n
m
o
n
o
c
y
t
e
s
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
We only recommend the depletion method. For Example, the RosetteSep™ Isolation Kit for human monocytes [Stem Cell Technologies, Cat. No 15028]. The advantage of depletion is that the monocytes are left untouched by antibodies during the process.
D
o
e
s
i
t
m
a
t
t
e
r
i
f
t
h
e
P
B
S
u
s
e
d
f
o
r
m
o
n
o
c
y
t
e
e
n
r
i
c
h
m
e
n
t
c
o
n
t
a
i
n
s
c
a
l
c
i
u
m
a
n
d
m
a
g
n
e
s
i
u
m
,
i
f
t
h
e
c
e
l
l
s
s
h
o
u
l
d
b
e
u
s
e
d
l
a
t
e
r
i
n
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
The PBS should be calcium and magnesium free to prevent clumping of cells. We routinely use PBS without calcium and magnesium for example Lonza Cat. No. 17-516F
H
o
w
c
a
n
I
d
e
t
e
r
m
i
n
e
t
h
e
c
o
r
r
e
c
t
c
e
n
t
r
i
f
u
g
a
t
i
o
n
s
p
e
e
d
f
o
r
m
y
p
a
r
t
i
c
u
l
a
r
c
e
n
t
r
i
f
u
g
e
a
n
d
r
o
t
o
r
?
The correct rotor speed can be calculated by measuring the maximum radius of your rotor and entering the information into the table found on the website which can be reached by clicking on the Related Link.
PREVIOUS
PAGE
1
PAGE
2
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
NEXT