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What are the suggested culturing conditions for CD34+ cells?

Lonza's CD34+ cells should be cultured according to Lonza's "Technical information and Instructions for Culturing Human CD34+ Cells"Upon thaw, we typically suggest that CD34+ cells be established in culture at a density of 20,000 to 200,000...

Why must Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) be re-differentiated to express collagen?

Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) are differentiated cells which express collagen when they are initially isolated, however, they lose their differentiated phenotype after serial expansion in monolayer culture so that by the 2nd...

Is the Human Leukocyte Antigen (HLA) type information available for any of Lonza's cell products?

Lonza does obtain the Human Leukocyte Antigen (HLA) type information for many (but not all) of the Clonetics® and Poietics® Cell Types we are offering. If you require cells of a specific HLA type donor, please contact Lonza's Scientific Support Team....

Will Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) express collagen Type X? Are Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) hypertrophic following re-differentiation?

Lonza guarantees that the Normal Human Articular Chondrocytes (NHAC-kn) will express collagen Type II and sulfated proteoglycans (can be stained with Alcian Blue) upon re-differentiation. Lonza’s recommended differentiation protocol does induce...

How are Lonza's CD34+ cells isolated?

Lonza's CD34+ cells are isolated first by obtaining mononuclear cells from bone marrow or cord blood via density gradient centrifugation. The CD34+ cells are then isolated form the mononuclear cell population using positive immunomagnetic selection.

What is the purity of Lonza's CD34+ cells?

Cell purity (as determined via FACS analysis for CD34 expression) is guaranteed to be 90% or greater for all Lonza CD34+ cell products. For some Lonza CD34+ cell products, a "budget" version of the product is available. The "budget" versions of the...

What is the distance between the two combs on Lonza's Reliant™ Gel, catalog number 57236?

From the bottom of one well to the top of the next, the distance is 31mm.

Where are Lonza's Bronchial Smooth Muscle Cells isolated from?

Lonza’s Bronchial Smooth Muscle Cells (both normal and diseased) are isolated from the bronchial tubes. The region used is around the bifurcation of the bronchial tubes to the lungs and small sections of the bronchia and trachea just off of the...

Can plasma or serum be run on Lonza's FlashGel™ System without prior purification?

No. The DNA or RNA will need to be extracted and purified before the samples can be loaded and run on the FlashGel™ Cassettes.

How are Lonza's mononuclear cells isolated?

Lonza's mononuclear cells are isolated from bone marrow, cord blood, or peripheral blood via density gradient centrifugation
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