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What is the recommended media for culturing Lonza's bone marrow stromal cells?

The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.

With regards to mesenchymal stem cells, what are "Dexter cultures"?

Dexter culture refers to the method of isolating stem cells proposed by Dexter et al in their 1977 paper: Dexter TM, Allen TD, Lajtha LG. Conditions controlling the proliferation of haemopoietic stem cells in vitro. JCell Physiol 1977; 91:...

What cells make up the population of cells in Lonza's bone marrow stromal cells?

The stromal layer is composed of many cell types including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells, and macrophages.

What is the recommended culturing protocol for Lonza's bone marrow stromal cells (2M-302)?

Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm sq in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...

What is the component labeled "MCGS" in the mesenchymal stem cell growth and differentiation media?

Mesenchymal Cell Growth Supplement (MCGS) is 100% fetal bovine serum prescreened to neither induce nor-prevent differentiation of human bone marrow derived stem cells.

How can I delete the data from the CytoSMART cloud?

It is not possible to delete data. You can download the data to a local drive or server, but there is no way to delete them from the cloud.

What is the difference between the various mesenchymal stem cell growth and differentiation basal media?

The basal media portions of the MSCGM growth and differentiation media are exactly the same except for the volume and the concentration of glucose. The glucose concentration in the growth (PT-3238) and osteogenic differentiation (PT-3924) basal media...

What is the CytoSMART System?

The CytoSMART™ System is a mini microscope that is designed to be placed inside a CO2 incubator to monitor cell cultures. Images are almost real time analyzed using image analysis algorithms to provide insights into the cell confluency.

How does the CytoSMART Lux 10X System work?

The CytoSMART™ Lux 10x System consists of three parts: a mini microscope, a tablet and online data storage and analysis (CytoSMART Connect Cloud). The mini microscope is placed inside the CO2 incubator. It is connected, through a cable that goes...

Do mesenchymal stem cells cultured in TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium (MSCGM-CD) require an extracellular matrix/plate coating?

While, cells may be able to be cultured in TheraPEAK™ Chemically Defined Mesenchymal Stem Cell Growth Medium (MSCGM-CD™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative...
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