Data Type
Cell Information
(858)
Citations
(4968)
FAQ
(515)
Culture Media
(61)
Category
Primary Cells and Media
(200)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin Detection
(54)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(135)
Cancer Research/Cell Biology
(77)
Immunotherapy / Hematology
(63)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(22)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(14)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
515 results sorted by
relevance
alphabetical
newest first
oldest first
A
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
,
h
o
w
d
o
y
o
u
d
e
t
e
r
m
i
n
e
c
e
l
l
v
i
a
b
i
l
i
t
y
?
We determine cell viability after Nucleofection® in two ways: 1) FACS determination of viable/dead cells by PROPIDIUM IODIDE STAINING. We normally analyze transfection efficiency in living cells by FACS: We first exclude cellular debris by...
I
s
t
h
e
r
e
a
d
i
f
f
e
r
e
n
c
e
b
e
t
w
e
e
n
,
f
o
r
e
x
a
m
p
l
e
,
t
h
e
p
r
o
g
r
a
m
s
C
-
2
0
a
n
d
C
-
0
2
0
?
No, the programs C-20 and C-020 are identical regarding their electrical parameters. The letter minus a two-digit number is the nomenclature of the Nucleofector™ I. Since we have launched the Nucleofector™ II we changed it to a letter minus a three...
A
r
e
y
o
u
r
N
u
c
l
e
o
f
e
c
t
o
r
®
S
o
l
u
t
i
o
n
s
c
h
e
c
k
e
d
f
o
r
R
N
a
s
e
a
c
t
i
v
i
t
y
d
u
r
i
n
g
y
o
u
r
Q
C
p
r
o
c
e
s
s
?
Yes. We do test the Solutions with a real time RT-PCR based assay. This assay basically measures the quantity of an RNA template after incubation in our solution. In case there is RNAse contamination the template would be digested which would be...
W
h
y
d
o
y
o
u
r
e
c
o
m
m
e
n
d
7
A
A
D
i
n
s
t
e
a
d
o
f
P
I
s
t
a
i
n
i
n
g
f
o
r
c
e
l
l
d
e
t
e
r
m
i
n
a
t
i
o
n
i
n
m
o
u
s
e
m
a
c
r
o
p
h
a
g
e
s
?
The results obtained using 7AAD staining are much clearer than those obtained using propidium iodide (PI). Using flow cytrometry stained and unstained, transfected and non-transfected populations can be separated much more easily.
W
h
a
t
i
s
t
h
e
o
p
t
i
m
a
l
s
i
z
e
o
f
D
N
A
t
h
a
t
y
o
u
r
e
c
o
m
m
e
n
d
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
We routinely use plasmids of 4-7 kb in our laboratories and plasmids up to approximately 20kb should not be a problem. Using plasmids larger than this will most likely result in lower transfection efficiency. Some preliminary results we have also...
H
o
w
l
o
n
g
h
a
v
e
y
o
u
b
e
e
n
a
b
l
e
t
o
o
b
s
e
r
v
e
G
F
P
e
x
p
r
e
s
s
i
o
n
i
n
p
r
i
m
a
r
y
H
u
m
a
n
K
e
r
a
t
i
n
o
c
y
t
e
s
?
GFP expression has been observed for up to one week as measured by light and fluorescence microscopy.
H
o
w
s
h
o
u
l
d
I
p
u
r
i
f
y
m
y
D
N
A
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
o
f
n
e
u
r
o
n
s
?
The quality and the concentration of DNA used for Nucleofection® in general plays a central role for the efficiency of gene transfer. We strongly recommend using endotoxin free prepared DNA. Endotoxin free Kits are available from several...
W
h
a
t
k
i
n
d
o
f
b
u
f
f
e
r
d
o
y
o
u
r
e
c
o
m
m
e
n
d
f
o
r
r
e
s
u
s
p
e
n
d
i
n
g
m
y
s
i
R
N
A
?
Please use the buffer that is recommended by your siRNA manufacturer.
W
h
a
t
s
t
o
c
k
c
o
n
c
e
n
t
r
a
t
i
o
n
o
f
P
r
o
p
i
d
i
u
m
I
o
d
i
d
e
d
o
e
s
L
o
n
z
a
u
s
e
f
o
r
F
A
C
S
a
n
a
l
y
s
i
s
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
We use a stock concentration of 100 µg/ml in PBS. This is diluted 1:300 – 1:400 into the sample.
I
'
v
e
n
o
t
i
c
e
d
t
h
a
t
d
u
r
a
t
i
o
n
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
i
o
n
®
P
r
o
g
r
a
m
s
e
e
m
s
t
o
c
h
a
n
g
e
e
v
e
n
w
h
e
n
I
h
a
v
e
n
'
t
c
h
a
n
g
e
d
t
h
e
p
r
o
g
r
a
m
I
a
m
u
s
i
n
g
.
W
h
y
?
The duration time of a program is dependent on the number of the program. The duration is also dependent on how long the Nucleofector® System has been turned on as well as the number of Nucleofections you have done in the last couple of...
PREVIOUS
PAGE
1
PAGE
2
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
NEXT