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What are SingleQuots Supplements and Growth Factors?

SingleQuots™ Supplements and Growth Factors contain several vials that may include growth factors, FBS, and antibiotics in pre-measured aliquots designed to be added to a bottle of basal medium. The concentrations of these factors are proprietary....

Is it possible to store reconstituted MycoAlert/MycoAlert PLUS Reagent and Substrate?

For optimal assay conditions, reconstituted reagent and substrate should be used fresh within 2 hours after reconstitution. During this time they can be kept at room temperature. For the MycoAlert™ Kit: Unused, reconstituted components can be...

Do I need to add antibiotics to your Clonetics Media? What is GA-1000?

For most Clonetics™ Media, gentamicin-amphotericin-B mixture is provided at a final concentration of 30 µg/ml gentamicin and 15 ng/ml amphotericin-B. This antibiotic mixture is provided in a red-capped vial labeled GA-1000. Antibiotics are not...

What type of sample do I need for the MycoALert Assay? Can it be stored?

The MycoAlert™ Assay requires the use of cell free supernatant only! Ideally you should sample your cell cultures and test immediately. If needed you can store the supernatant at room temperature for 8 hours, 2°C-8°C for 1 week. The MycoAlert™...

Can I use a medium other than the one you offer to culture Clonetics Cells?

We have an optimized medium for each Clonetics™ Cell type. The cells are grown and tested in their specific medium system. We cannot guarantee cell performance if our recommended medium is not used.

How much sample do you need for accurate results with MycoAlert Assay? Do you need cells or just supernatant?

The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.

What is the difference between the EGM BulletKit [CC-3124] and the EGM-2 BulletKit [CC-3162]?

The primary difference between the two media types is in the growth factors. The EGM™ BulletKit™ contains: BBE, hEGF, hydrocortisone, FBS, and GA-1000. In the EGM™-2 BulletKit™ we have removed the BBE and replaced it with known growth factors: VEGF,...

Is it possible to quantify the mycoplasma with the MycoAlert Assay?

No, the MycoAlert™ Assay is not quantitative; it has been designed to give a simple Yes or No answer, pertaining to the presence of Mycoplasma.

What kit sizes are available for the 4D-Nucleofector X Unit?

We offer two different kit sizes for the 100 µl Nucleocuvette™ Format (12 rxn and 24 rxn) and one size for the 20 µl 16-well Nucleocuvette™ Strip Format (32 rxn). Additionally there are volume discounts availble.

When performing the MycoAlert Assay, why is it necessary to spin out the cells?

It is highly recommended that the samples are spun down prior to testing. If cells are present in the assay they will contribute to the initial reading producing a higher background. This in turn can decrease the difference between the background and...
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