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Where are Lonzas aortic adventitial fibroblasts (AoAF) isolated from?

Lonza’s aortic adventitial fibroblasts (AoAF) are isolated from the outer connective tissue layer of the aorta. The aortia is divided into three layers. The tunica intima is the inner endothelial layer, the tunica media is the layers of the smooth...

What is the difference between the EGM and EGM-MV Growth Media? What is the difference between the EGM-2 and EGM-2MV Growth Media?

Lonza endothelial cell media ending with –MV (such as the EGM™-MV and EGM™-2MV Growth Media) are typically designated for use with microvascular endothelial cells (with some exceptions).Lonza endothelial cell media not ending with –MV (such as the...

Where are Lonzas lung fibroblasts (NHLF and DHLF) isolated from?

Lonza’s lung fibroblasts are not taken from any specific area of the lung but instead are taken from an area that appears to be in good shape and is non-hemorrhagic. The pleural membrane is removed and the peripheral tissue is used. Enzymatic...

Which isotype of VEGF is used in the EGM®-2 and EGM®-2MV Growth Media?

The isotype of VEGF is used in the EGM®-2 and EGM®-2MV Growth Media is isotype 165.

Where are Lonzas dermal fibroblasts (NHDF) isolated from?

Lonza’s offers dermal fibroblasts from both adult and neonatal donors. Lonza’s adult dermal fibroblasts (NHDF-Ad) are usually isolated from tissue obtained from elective cosmetic surgical procedures. Generally, these procedures are abdominoplasties...

What is the concentration of sugar in Lonzas endothelial cell media?

While the complete formulation of all of Lonza’s specialty media are proprietary, the EBM™, EBM™-2, and EBM™ phenol red free basal media will all contain D-glucose (dextrose) [CAS number 50-99-7] at exactly 1.0 g/L (5.55 mM). There are no other...

Do I need a special license when using the Nucleofector® Technology for commercial purposes?

Each Nucleofector® Device (4D-Nucleofector® System, Nucleofector® 2b Device, HT Nucleofector® System) comes with a research license. Research license terms are included in the instrument manual. The research license is...

Where is Lonza's fresh bone marrow collected from?

The bone marrow is collected from the iliac crest.

What is the difference between each of the types of HUVECs that Lonza offers?

When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...

What type of heparin is used with fresh bone marrow?

Lonza uses sodium heparin as the anticoagulant at a final concentration of 100 units/mL.