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Can I use the Nucleofector™ Technology for RNAi applications? How do I start?

Yes. The Nucleofector™ Technology can be easily applied for any RNAi substrate (siRNA, shRNA, miRNA). You can use the same conditions described in the cell type specific Optimized Protocol for DNA vectors (cDNA, shRNA or miRNA expressing plasmids) or...

Can I use the Nucleofector™ 2b Solutions and Optimized Protocols with the 4D-Nucleofector™ X-Unit or the 96-well Shuttle™ Device?

No. The 4D-Nucleofector and 96-well Shuttle™ System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle™ Solutions are available for primary cells. For cell lines, three different 96-well...

Can I order any of the Nucleofector™ Kit components separately?

No, we do not offer the components of the Nucleofector Kits separately. The Nucleofector™ Kits are only available as complete kits.

Who is the distributor for my country?

Please click on the link provided to search for your distributor.

Why do you have different Optimized Protocols for the Nucleofection™ of unstimulated and stimulated Human T cells?

We found that the stimulation of T cells not only changes the cells' function but also significantly alters the requirements for successful Nucleofection™. That's why Lonza developed two Optimized Protocols for transfection giving different...

Can I use a Nucleofector™ I or Nucleofector™ 2b Device together with the 96-well Shuttle™ Device?

No, the 96-well Shuttle™ only works in conjunction with the Nucleofector™ IIs Device or our 4D-Nucleofector™ Device.

Is it possible to use frozen primary CD34+ hematopoietic stem cells for Nucleofection™?

Yes. For cryopreserved PBMCs or enriched CD34 populations, we recommend culturing the thawed cells 1-2 hours in culture medium before Nucleofection™. Any further enrichment procedure after thawing is not recommended. Viability of frozen nucleofected...

Is it possible to use different substrates (e.g. siRNA duplexes) with the 96-well Shuttle™ Device?

Yes. Transfection can be done with any substrate AND under exactly the same conditions. For example, the same conditions (96-well Shuttle™ Solution and program) are used for siRNA transfection or shRNA vectors. This greatly facilitates the...

What are the critical steps for successful Nucleofection™ of monocytic cell lines like THP-1, HL60 and U-937?

To achieve optimal results, we strongly recommend following the Optimized Protocols in regard to cell culture conditions (medium, splitting cycle, density before Nucleofection), DNA amount and purity. Please also be sure not to exceed 90xg when...

What is the advantage of the 96-well Shuttle™ Device over common lipofection, especially in a high throughput framework?

Several primary cells of high research relevance can simply not be transfected at relevant efficiencies and viabilities using lipofection. The same holds true for many suspension cell lines which are often used for protein production. These difficult...

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