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689 results sorted by
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During cryopreservation, cells may lyse leaching DNA into the culture suspension. The sticky DNA, if not treated, will bind cells together causing clumping. DNase I functions to remove the leached DNA and prevent clumping of culture cells, and...
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Lonza Research & Development and Quality Control have seen that glial fibrillary acidic protein (GFAP) expression decreases rapidly with passaging. Lonza tests GFAP expression in the first passage from cryopreservation and can therefore only...
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Lonza’s melanocytes, both adult and neonatal, are currently only classified by the donor’s race. Lonza designates race as Caucasian (C), Black/African American (B), Asian (A), Hispanic (H), Other (O) (which can include Indian, Native American,...
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Adult melanocytes typically have a very slow doubling time, especially in comparison to neonatal melanocytes. Endothelin-3 (ET-3) is added to adult melanocytes only to improve the doubling time and, in the process, help to maintain the cells as...
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Whole, unprocessed bone marrow typically contains 4 million to 10 million mononuclear cells per milliliter (mL).
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Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial cells being isolated in the HMEC product.
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Lonza's CD34+ cells should be thawed according to the "Procedure for thawing Poietics™ cells". Upon thaw, we typically suggest that CD34+ cells be established in culture at a density of 20,000 to 200,000 cells per ml of Lonza’s HPGM (or a comparable...
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Unfortunately, Lonza has not thoroughly investigated the origin of the mammary epithelial cells being isolated in the HMEC product. Luminal mammary epithelial cells have been specifically isolated in the past, however, luminal mammary epithelial...
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Lonza does not obtain the Human Leukocyte Antigen (HLA) type information for any of our standard research grade products. If you require cells of a specific HLA type donor, please contact Lonza's Cells on Demand (cellsondemand@lonza.com) to inquire...
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Lonza's CD34+ cells are isolated first by obtaining mononuclear cells from bone marrow or cord blood via density gradient centrifugation. The CD34+ cells are then isolated form the mononuclear cell population using positive immunomagnetic selection.
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