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What is the purpose of DNAse I in the "Procedure for thawing Poietics cells"?

During cryopreservation, cells may lyse leaching DNA into the culture suspension. The sticky DNA, if not treated, will bind cells together causing clumping. DNase I functions to remove the leached DNA and prevent clumping of culture cells, and...

When culturing Lonzas normal human astrocytes, will glial fibrillary acidic protein (GFAP) continue to be expressed throughout serial passages?

Lonza Research & Development and Quality Control have seen that glial fibrillary acidic protein (GFAP) expression decreases rapidly with passaging. Lonza tests GFAP expression in the first passage from cryopreservation and can therefore only...

Are Lonzas melanocytes classified/categorized by the donors skin tone?

Lonza’s melanocytes, both adult and neonatal, are currently only classified by the donor’s race. Lonza designates race as Caucasian (C), Black/African American (B), Asian (A), Hispanic (H), Other (O) (which can include Indian, Native American,...

Why are Lonzas adult melanocytes cultured with Endothelin-3 (ET-3) while the neonatal melanocytes are not cultured with this reagent?

Adult melanocytes typically have a very slow doubling time, especially in comparison to neonatal melanocytes. Endothelin-3 (ET-3) is added to adult melanocytes only to improve the doubling time and, in the process, help to maintain the cells as...

How many mononuclear cells are typically found per milliliter (mL) of whole, unprocessed bone marrow?

Whole, unprocessed bone marrow typically contains 4 million to 10 million mononuclear cells per milliliter (mL).

Are Lonzas human mammary epithelial cells tested for estrogen receptor response?

Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial cells being isolated in the HMEC product.

What are the suggested culturing conditions for CD34+ cells?

Lonza's CD34+ cells should be thawed according to the "Procedure for thawing Poietics™ cells". Upon thaw, we typically suggest that CD34+ cells be established in culture at a density of 20,000 to 200,000 cells per ml of Lonza’s HPGM (or a comparable...

Are Lonzas human mammary epithelial cells luminal or basal in origin?

Unfortunately, Lonza has not thoroughly investigated the origin of the mammary epithelial cells being isolated in the HMEC product. Luminal mammary epithelial cells have been specifically isolated in the past, however, luminal mammary epithelial...

Is the Human Leukocyte Antigen (HLA) type information available for any of Lonza's cell products?

Lonza does not obtain the Human Leukocyte Antigen (HLA) type information for any of our standard research grade products. If you require cells of a specific HLA type donor, please contact Lonza's Cells on Demand (cellsondemand@lonza.com) to inquire...

How are Lonza's CD34+ cells isolated?

Lonza's CD34+ cells are isolated first by obtaining mononuclear cells from bone marrow or cord blood via density gradient centrifugation. The CD34+ cells are then isolated form the mononuclear cell population using positive immunomagnetic selection.
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