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Can MycoZap Prophlyactic be used in cell cultures expressing a Tet repressor?

One of the MycoZap™ Prophylactic components interacts with the Tet repressor, leading to unwanted expression of genes expressed under the control of a Tet response element in lines. Therefore, MycoZap™ Prophylactic is not suited for cell cultures...

Are your Nucleofector Solutions checked for RNase activity during your QC process?

Yes. We do test the Solutions with a real time RT-PCR based assay. This assay basically measures the quantity of an RNA template after incubation in our solution. In case there is RNAse contamination the template would be digested which would be...

What kind of buffer do you recommend for resuspending my siRNA?

Please use the buffer that is recommended by your siRNA manufacturer.

What stock concentration of Propidium Iodide does Lonza use for FACS analysis?

We use a stock concentration of 100 µg/ml in PBS. This is diluted 1:300 – 1:400 into the sample.

Can I use a Nucleofector I or Nucleofector 2b Device together with the 96-well Shuttle Device?

No, the 96-well Shuttle™ only works in conjunction with the Nucleofector™ IIs Device or our 4D-Nucleofector™ Device.

Is it possible to use different substrates (e.g. siRNA duplexes) with the 96-well Shuttle Device?

Yes. Transfection can be done with any substrate AND under exactly the same conditions. For example, the same conditions (96-well Shuttle™ Solution and program) are used for siRNA transfection or shRNA vectors. This greatly facilitates the...

What is the advantage of the 96-well Shuttle Device over common lipofection, especially in a high throughput framework?

Several primary cells of high research relevance can simply not be transfected at relevant efficiencies and viabilities using lipofection. The same holds true for many suspension cell lines which are often used for protein production. These difficult...

What are the contents of a 96-well Shuttle Kit?

As the system will most likely be used for high-throughput approaches we provide plates which are pre-equipped with 96-well Nucleocuvette™ modules (2x8). In addition to that, the 96-well Shuttle solution, the supplement, and the pmaxGFP™ are also...

What is the advantage of the Nucleofector Technology over common electroporation (systems)?

High cell viability and high transfection efficiency with the Nucleofection™ Technology are the most prominent features. Nucleofection™, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working with non-dividing...