Data Type
Cell Information
(855)
Citations
(4792)
FAQ
(704)
Culture Media
(92)
Category
Primary Cells and Media
(277)
Transfection
(186)
Laboratory Instrumentation
(70)
Endotoxin Detection
(57)
Electrophoreses and Analysis
(56)
Bioassay
(48)
3D Cell Culture
(42)
Mycoplasma Detection and Prevention
(34)
Serum-free and Speciality Media
(15)
Classical Media and Reagents
(11)
Cell Lines and Primary Cancer Cells
(9)
Uncategorized
(7)
Live Cell Imaging
(6)
Cell Services
(3)
Bioprocess Containers
(1)
Informatics for QC Microbiology
(1)
Protozoa
(1)
+ Show All
Research Area
Uncategorized
(174)
Basic Research
(129)
Cancer Research/Cell Biology
(85)
Immunotherapy / Hematology
(72)
Endotoxin Testing
(55)
Stem Cells
(53)
Molecular Biology
(40)
Neurobiology
(39)
Respiratory Research
(33)
Toxicology
(33)
Cardiovascular
(28)
Regenerative medicine
(25)
Dermatology/Tissue Engineering
(21)
Gene Expression
(16)
Drug Discovery
(7)
Gastroenterology
(4)
Parasitology
(3)
+ Show All
704 results sorted by
relevance
alphabetical
newest first
oldest first
W
h
a
t
s
e
l
e
c
t
i
o
n
m
a
r
k
e
r
s
c
a
n
I
u
s
e
f
o
r
s
t
a
b
l
e
t
r
a
n
s
f
e
c
t
i
o
n
s
?
The most commonly used marker is the neomycin phosphotransferase (neo) gene that confers resistance to G418 to eukaryotic cells. Other markers are Puromycin, Hygromycin, Zeocin, or the HPRT gene that can be used in HPRT-deficient cells.
W
h
a
t
i
s
t
h
e
a
d
v
a
n
t
a
g
e
o
f
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
D
e
v
i
c
e
o
v
e
r
c
o
m
m
o
n
l
i
p
o
f
e
c
t
i
o
n
,
e
s
p
e
c
i
a
l
l
y
i
n
a
h
i
g
h
t
h
r
o
u
g
h
p
u
t
f
r
a
m
e
w
o
r
k
?
Several primary cells of high research relevance can simply not be transfected at relevant efficiencies and viabilities using lipofection. The same holds true for many suspension cell lines which are often used for protein production. These difficult...
W
h
a
t
a
r
e
t
h
e
c
o
n
t
e
n
t
s
o
f
a
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
K
i
t
?
As the system will most likely be used for high-throughput approaches we provide plates which are pre-equipped with 96-well Nucleocuvette™ modules (2x8). In addition to that, the 96-well Shuttle solution, the supplement, and the pmaxGFP™ are also...
A
t
w
h
i
c
h
t
i
m
e
p
o
i
n
t
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
s
h
o
u
l
d
I
s
t
a
r
t
s
e
l
e
c
t
i
o
n
o
f
s
t
a
b
l
e
i
n
t
e
g
r
a
t
i
o
n
?
Antibiotics should be added 24-48 h after Nucleofection.
H
o
w
o
f
t
e
n
s
h
o
u
l
d
I
c
h
a
n
g
e
t
h
e
m
e
d
i
u
m
d
u
r
i
n
g
s
e
l
e
c
t
i
o
n
o
f
s
t
a
b
l
e
c
l
o
n
e
s
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
Every 2-3 days. This eliminates potentially toxic substances produced by dying cells and secondly, it keeps the concentration of the antibiotic at a constant level.
W
h
a
t
i
s
t
h
e
a
d
v
a
n
t
a
g
e
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
™
T
e
c
h
n
o
l
o
g
y
o
v
e
r
c
o
m
m
o
n
e
l
e
c
t
r
o
p
o
r
a
t
i
o
n
(
s
y
s
t
e
m
s
)
?
High cell viability and high transfection efficiency with the Nucleofection™ Technology are the most prominent features. Nucleofection™, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working with non-dividing...
H
o
w
m
a
n
y
s
t
a
b
l
e
c
l
o
n
e
s
w
i
l
l
I
g
e
t
f
r
o
m
o
n
e
t
r
a
n
s
f
e
c
t
i
o
n
?
This depends on the respective cell type (transfection efficiency, viability and integration frequency). If it is important to you to know the frequency of stable integrants before carrying out your actual experiments, the frequency should be...
I
s
i
t
p
o
s
s
i
b
l
e
t
o
p
r
e
-
p
l
a
t
e
s
u
b
s
t
r
a
t
e
s
w
h
e
n
u
s
i
n
g
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
™
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
Yes, pre-plating of substrates like siRNA and subsequent storage at -20°C is possible.
F
o
l
l
o
w
i
n
g
N
u
c
l
e
o
f
e
c
t
i
o
n
™
,
I
c
a
n
n
o
t
o
b
t
a
i
n
s
t
a
b
l
e
c
l
o
n
e
s
f
r
o
m
s
i
n
g
l
e
c
e
l
l
s
,
w
h
a
t
c
o
u
l
d
b
e
t
h
e
r
e
a
s
o
n
?
Some cell lines need certain cell densities to proliferate. For those cells you can try using conditioned medium for setting up limiting dilution or culturing single cells together with feeder cells.
W
h
a
t
k
i
n
d
o
f
p
r
o
t
o
c
o
l
y
o
u
r
e
c
o
m
m
e
n
d
f
o
r
s
t
i
m
u
l
a
t
i
o
n
o
f
h
u
m
a
n
T
c
e
l
l
s
?
For getting the results we present in our datasheet, we recommend the follwing strategy: Cells should be stimulated and cultivated for 3 days before Nucleofection, preferentially on plates coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibody....
PREVIOUS
PAGE
2
PAGE
3
PAGE
4
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
PAGE
10
NEXT