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703 results sorted by
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No, cryopreserved cells should be stored in liquid Nitrogen. Storing the cells at lower temperatures can irreversibly damage the cells.
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A population doubling is the total number of population doublings of a cell type since its initiation in vitro. A population doubling is calculated using the following equations:Cell count at day of passage /Cell count 24 hours after seeding =...
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The doubling time is the number of hours it takes for a cell population to double in number. Doubling time is calculated using the following equation:Number of hours from 24 hours after seeding until passage/Population Doublings = Doubling Time...
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No. Serum is toxic to the SAECs.
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There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process. However, the ciliated cells do not persist and are usually gone by the...
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Yes, see this list of part codes for the different types of transport boxes: Part code: AAK-1001Items: 4D-Nucleofector™ Demo Suitcase (2 units)Suited for: Core + X or Core + Y or X + YPart...
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Lonza's QC uses HCM (CC-3198) supplemented with 20% FBS and 25 mM Hepes. No plating media is necessary when seeding for spheroids.
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Use the guidelines we have given in the Quasi Vivo™ User Manual to find the range of possible flow rates. Set up the system and compare the viability of cells cultured for 24 hours (or some other appropriate time scale for your cell type) at each...
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The Nucleofector™ Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell-type...
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There are several reasons to choose the Nucleofector™ Technology for your gene transfer experiments. One is the direct transfer of DNA to the cell nucleus. This makes gene expression independent from cell division. Therefore, the technique is the...
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