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Can I store my cryopreserved primary cell ampule at 80°C?

No, cryopreserved cells should be stored in liquid Nitrogen. Storing the cells at lower temperatures can irreversibly damage the cells.

How do you calculate a population doubling?

A population doubling is the total number of population doublings of a cell type since its initiation in vitro. A population doubling is calculated using the following equations:Cell count at day of passage /Cell count 24 hours after seeding =...

How do you calculate doubling time?

The doubling time is the number of hours it takes for a cell population to double in number. Doubling time is calculated using the following equation:Number of hours from 24 hours after seeding until passage/Population Doublings = Doubling Time...

Can serum be used with Lonza's SAECs?

No. Serum is toxic to the SAECs.

Are ciliated cells present in the SAECs?

There are usually ciliated cells present in the SAEC isolations, which shows that the tissue is good and healthy and that everything went well in the isolation process.  However, the ciliated cells do not persist and are usually gone by the...

Do you also sell the boxes or cases for the Nucleofector Core, X, Y and LV units? 

Yes, see this list of part codes for the different types of transport boxes: Part code: AAK-1001Items: 4D-Nucleofector™ Demo Suitcase (2 units)Suited for: Core + X or Core + Y or X + YPart...

What media should be used for spheroid formation with hepatocytes?

Lonza's QC uses HCM (CC-3198) supplemented with 20% FBS and 25 mM Hepes. No plating media is necessary when seeding for spheroids. 

How do I optimize for the flow rate of my cell type in Quasi Vivo? 

Use the guidelines we have given in the Quasi Vivo™ User Manual to find the range of possible flow rates. Set up the system and compare the viability of cells cultured for 24 hours (or some other appropriate time scale for your cell type) at each...

What is the basic principle of the Nucleofector Technology?

The Nucleofector™ Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell-type...

Why is the Nucleofector Technology ideal for primary cells and difficult-to-transfect cell lines?

There are several reasons to choose the Nucleofector™ Technology for your gene transfer experiments. One is the direct transfer of DNA to the cell nucleus. This makes gene expression independent from cell division. Therefore, the technique is the...
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