Data Type
Cell Information
(876)
Citations
(5046)
FAQ
(541)
Culture Media
(62)
Category
Primary Cells and Media
(199)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin and Pyrogen Testing
(70)
Bioassay
(45)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(25)
Cell Lines and Primary Cancer Cells
(10)
Classical Media and Reagents
(7)
3D Cell Culture
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(137)
Cancer Research/Cell Biology
(79)
Immunotherapy / Hematology
(62)
Gene Expression
(57)
Endotoxin Testing
(49)
Uncategorized
(46)
Stem Cells
(41)
Cardiovascular
(27)
Respiratory Research
(27)
Molecular Biology
(26)
Neurobiology
(26)
Toxicology
(23)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(15)
Drug Discovery
(8)
Parasitology
(3)
Gastroenterology
(2)
+ Show All
541 results sorted by
relevance
alphabetical
newest first
oldest first
C
a
n
I
a
p
p
l
y
m
o
r
e
t
h
a
n
o
n
e
p
r
o
g
r
a
m
(
t
r
a
n
s
f
e
c
t
i
o
n
c
o
n
d
i
t
i
o
n
)
p
e
r
p
l
a
t
e
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
S
y
s
t
e
m
?
With the 96-well Shuttle® system, it is possible to apply up to 96 different programs (transfection conditions) per 96-well Nucleocuvette® Plate. Therefore, optimization of Nucleofection® Conditions for a particular cell line can be...
I
w
a
n
t
t
o
s
t
i
m
u
l
a
t
e
h
u
m
a
n
p
r
i
m
a
r
y
T
-
c
e
l
l
s
w
i
t
h
a
n
t
i
-
C
D
3
a
n
d
a
n
t
i
-
C
D
2
8
.
D
o
y
o
u
h
a
v
e
a
d
e
t
a
i
l
e
d
p
r
o
t
o
c
o
l
f
o
r
t
h
i
s
?
Yes, the detailed protocol is part of our 4D-Nucleofector® Protocol for stimulated Human T Cells.For optimal stimulation of the transfected cells we recommend coating 96-wells plates for stimulation [Nunc Immuno™ Plate C96 Maxi Sorp™, Cat. No. 430...
W
h
i
c
h
r
e
p
o
r
t
e
r
g
e
n
e
s
h
o
u
l
d
I
c
h
o
o
s
e
f
o
r
m
y
r
e
p
o
r
t
e
r
g
e
n
e
e
x
p
e
r
i
m
e
n
t
?
In general, all reporter genes can be used.For some applications (e.g. reporter gene experiments in various primary cells, experiments with late analysis time point due to starving and /or stimulation) a reporter gene with a longer half life than...
W
h
a
t
i
s
t
h
e
a
m
o
u
n
t
o
f
D
N
A
I
c
a
n
u
s
e
i
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
®
E
x
p
e
r
i
m
e
n
t
?
This totally depends on the Nucleofector® system you are working with. While in the 100 µL reaction vessels usually 1 - 5 µg DNA is being used, for the 20 µL reaction vessels (e.g. X Unit, 96-well Unit and HT...
W
h
e
n
s
h
o
u
l
d
I
b
e
g
i
n
l
o
o
k
i
n
g
f
o
r
t
h
e
L
u
c
i
f
e
r
a
s
e
s
i
g
n
a
l
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
The time course of plasmid expression after Nucleofection® might be different from the time course seen with other transfection methods. We recommend looking at different time points and start with the first time point as early as 4 to 6 hours post...
W
h
a
t
s
h
o
u
l
d
I
p
a
y
a
t
t
e
n
t
i
o
n
t
o
w
h
e
n
t
r
a
n
s
f
e
r
r
i
n
g
m
y
r
e
p
o
r
t
e
r
g
e
n
e
s
e
t
u
p
f
r
o
m
a
c
e
l
l
l
i
n
e
t
o
a
p
r
i
m
a
r
y
c
e
l
l
?
In general the overall expression level differs between different cell types. Don't expect the same level of gene induction when working with primary cells. You might need to change to other DNA amounts or ratios.
D
o
y
o
u
h
a
v
e
d
a
t
a
a
b
o
u
t
t
h
e
i
n
v
i
v
o
i
m
p
l
a
n
t
a
t
i
o
n
o
f
h
e
p
a
t
o
c
y
t
e
s
t
r
a
n
s
f
e
c
t
e
d
b
y
N
u
c
l
e
o
f
e
c
t
i
o
n
™
?
F
o
r
h
o
w
l
o
n
g
c
o
u
l
d
p
r
o
t
e
i
n
e
x
p
r
e
s
s
i
o
n
b
e
d
e
t
e
c
t
e
d
?
There is a publication reporting on the successful reinjection of modified hepatocytes into mice.Chen et al. were able to monitor the expression over several days. This group worked with a preliminary test solution for mouse hepatocyte as the...
W
h
a
t
i
s
t
h
e
f
u
n
c
t
i
o
n
o
f
r
e
t
i
n
o
i
c
a
c
i
d
i
n
t
h
e
B
E
G
M
®
m
e
d
i
a
?
In vivo, bronchial epithelial cells differentiate along an abnormal squamous pathway under conditions of retinoid deficiency. The squamous phenotype is characterized by the induction of specific markers such as keratin 13, and the enzymes...
A
r
e
r
a
t
a
n
d
m
o
u
s
e
h
e
p
a
t
o
c
y
t
e
s
t
r
a
n
s
f
e
c
t
e
d
b
y
N
u
c
l
e
o
f
e
c
t
i
o
n
®
s
t
i
l
l
f
u
n
c
t
i
o
n
a
l
?
Yes, transfected mouse hepatocytes show albumin synthesis rates comparable to those of untransfected cells. Moreover the morphology and polarization of rat hepatocytes is not affected by Nucleofection®.
W
h
a
t
a
r
e
m
y
c
o
p
l
a
s
m
a
s
?
Mycoplasmas belong to the family Mollicutes, which includes Acholeplasma, Ureaplasma and other species.However the term "Mycoplasma" is most often used as a "cover-all".More than 180 species have been identified of which 20 distinct Mycoplasma...
PREVIOUS
PAGE
5
PAGE
6
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
PAGE
12
PAGE
13
NEXT