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What is the maximum height flasks can be for use with Lonza's CytoSMART™ Device?

In general, when using Lonza's CytoSMART™ Device, the flask needs to be below 4.5 cm in height. Most culture flasks (including T-25, T-75, and T-150 flasks) will be acceptable. For larger flasks, such as T-162 flasks, Corning/Costar T-162 Flasks are...

The Instructions for Thawing Poietics™ cells recommends adding DNAse I. Do cells need to be cultured using DNAse I? When can I remove DNAse I?

DNAse I is only added to prevent clumping during the initial thawing and wash steps for Poietics™ Cells. Once the cells receive their final wash and are centrifuged, during the final resuspension, cells can be suspended in their regular culture...

Is the CytoSMART™ Device able to view multiple positions in a multi-well plate?

No, the CytoSMART™ Device has fixed lenses and does not have an automated x,y-state to move a multi-well plate. You can compare culture groups (e.g. control group and treated group) by using multiple CytoSMART™ Systems.

Can I plate cells at a higher initial concentration than the Clonetics™ and Poietics™ plating protocol recommends? What is the minimum dilution of DMSO most cells can tolerate?

Lonza's recommended plating density for primary cells is established for two main reasons: 1. To ensure residual DMSO is sufficiently diluted while cells are initially attaching and 2. to allow a maximum number of population doublings per passage....

How can I disinfect the CytoSMART™ Device?

There are several ways of disinfecting the CytoSMART. The following reagents have been tested without a negative effect on the coating:Ethanol 80%, Bacillol AF, Sterilium Virugard, Isopropanol, Incidin plus (2%), Lysol (5.5% o-Benzyl-p-chlorophenol),...

What is the composition of the L7™ hPSC Matrix?

Lonza’s L7™ hPSC Matrix is a xeno-free and defined recombinant protein. Unfortunately, further details regarding the composition of this reagent are proprietary.

What are the dimensions of a the CytoSMART™ Device?

The CytoSMART is 133mm x 90 mm x 100 mm (LxWxH) or roughly the same footprint as a 96-well plate.

Can I connect multiple CytoSMART™ Units to one tablet?

No. Each CytoSMART™ Unit comes with a dedicated tablet.

For generating iPSCs from PBMCs it is recommended to do a priming step to enrich the erythroblast population. Why not use T-cell or CD34+ population?

It is not ideal to reprogram T-cells because of pre-existing DNA rearrangements at the V(D)J locus. While CD34+ cells work well for generating iPSCs, they are in very low numbers in the PBMC population. Erythroblasts are in relatively high numbers in...

Does Lonza have references for the CytoSMART™ Lux 10X?

Yes. CytoSMART™ Lux 10X is being used by several leading hospitals, universities and biopharmaceutical companies. Please contact your local sales representative for additional information or visit our webpage

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