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679 results sorted by
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D
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F
P
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e
q
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?
Yes. The coding sequence ends with a stop codon (TGA/UGA).
W
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k
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N
A
?
Please use the buffer that is recommended by your siRNA manufacturer.
W
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R
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x
p
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y
p
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F
P
-
R
e
d
?
Most of the commercial antibodies for GFP do not detect GFP expressed from pmaxGFP or pmaxFP-Green vectors. The origin of the protein expressed by the pmaxGFP control plasmid and pmaxFP-Green is from the green fluorescent protein CopGFP, cloned from...
W
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m
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G
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p
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p
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G
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P
™
p
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a
s
m
i
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?
The molecular weight of the protein is about 27kDa.
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A
C
S
a
n
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l
y
s
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s
?
We use a stock concentration of 100 µg/ml in PBS. This is diluted 1:300 – 1:400 into the sample.
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9
6
-
w
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S
h
u
t
t
l
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™
k
i
t
s
?
The shelf life of the 96-well Shuttle™ Kits is 24 months from date of manufacturing. We guarantee a remaining shelf life of at least 6 months after sending them out of the warehouse.
C
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B
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M
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T
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4
°
C
.
I
s
t
h
i
s
n
o
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m
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l
?
Yes, it is normal for component B to become solid at 4°C. The crystals dissolve at room temperature and functionality of component B is not affected.
D
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y
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a
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N
A
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5
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n
M
?
Yes. Please click on the attached Technical Reference Guide: "Designing an RNAi Experiment Using Nucleofection™". On page 3 you can find a table with some examples.
T
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p
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m
e
?
Isolated hepatocytes can be transported in Krebs-Henseleit Buffer or supplemented William's E Medium. Long transport time for isolated hepatocytes, (in excess of one hour) can cause a decrease in efficiency of Nucleofection™, but does not seem to...
H
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D
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N
u
c
l
e
o
f
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c
t
o
r
™
?
We have used up to 6 µg of DNA per 100 µl reaction with no deleterious effect.
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