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What cell number do I need per well when using the 96-well Shuttle Device or the 4D-Nucleocuvette Stripes?

The cell number is very much dependent on the cell type you use. For high throughput Nucleofection™, we usually start with one fifth of the optimal amount recommended for the standard Nucleofection™ in the 100µl cuvette. Currently the absolute cell...

How many cortical neurons can I expect from a single pup?

According to QBM Cell Sciences, you can expect 12 million cortical neurons from an average E18-E19 rat pup. You can expect 4 million cortical neurons from an average mouse pup.

What is the amount of DNA needed per well when using the 96-well Shuttle Device?

The amount depends on the cell type and on the DNA construct. As a rule of thumb 400 ng of DNA per well should be applied. However, for some cells or constructs even 100 ng may be sufficient.

What is the amount of siRNA needed per Nucleofection Reaction?

When performing siRNA-mediated knockdown experiments it is advisable to conduct a dose-response (concentration) analysis to determine the minimum siRNA concentration necessary for sufficient target knockdown on the mRNA, protein, or functional level....

Does the 96-well Shuttle recognize if the cuvette plate is in backwards?

No, it does not recognize the orientation of the plate. Please make sure that well A1 is positioned in the upper left corner of the retainer.

Which pipette tips are compatible with the 96-well Shuttle Nucleocuvette Plates?

We recommend using special tips, e.g. epT.I.P.S™ (Eppendorf, Hamburg, Germany) or TallTips™ (Matrix Technologies, Hudson, NH, USA). You can contact our Scientific Support Team for a list of compatible tips, including tips for pipetting robots.

Do any of the three instruments used in the 96-well Shuttle System "go to sleep" or power off automatically?

The energy options of the laptop are set as such that only the screensaver is activated, the harddisk won't turn down and hibernation is disabled. One can change these settings via the system control. However this is not recommended because by...

Can I freeze the 96-well Nucleocuvette Plates?

Yes. Freezing of the plates at -20°C does not alter their properties.

Can I integrate the 96-well Shuttle Device into a liquid handling system?

Yes. The 96-well Shuttle™ Device is designed in such a way that it is addressable by robot grippers. Furthermore, the software contains the required interface to be connected with the LHS software. However, the practical implementation for each...

Can I use the Nucleofector 2b Solutions and Optimized Protocols with the 4D-Nucleofector X-Unit or the 96-well Shuttle Device?

No. The 4D-Nucleofector and 96-well Shuttle™ System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle™ Solutions are available for primary cells. For cell lines, three different 96-well...
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