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528 results sorted by
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This message means that the device cut off the pulse before it was completed (usually this is because the conductivity of the solution is at the low end of the normal range). The pulse was applied; it is just going to be a little weaker than...
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There is a publication reporting on the successful reinjection of modified hepatocytes into mice.Chen et al. were able to monitor the expression over several days. This group worked with a preliminary test solution for mouse hepatocyte as the...
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In the back of the respective manuals, there is a chart giving a brief description of the error codes. If more information is needed or an error code is recurrent, it is best to contact Scientific Support for advice to determine if there is a...
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Yes, transfected mouse hepatocytes show albumin synthesis rates comparable to those of untransfected cells. Moreover the morphology and polarization of rat hepatocytes is not affected by Nucleofection™.
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The Nucleofector® II was tested by TÜV Rheinland product safety GmbH and found to be in compliance with the following standards: IEC 61010-1:2001 (2nd edition) and EN 61010-1:2001 (2nd edition). TÜV Rheinland of North America Inc. tested the...
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Yes, this is a routine observation.Fluorescent fusion proteins can appear less bright than the fluorescent protein itself. This may be due to differences in protein folding of the fused protein.
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It is very critical. Waiting too long for analysis post transfection can lead to loss of peak expression. The optimal time for analysis is related to two factors: stability of protein being expressed and transfection method. For a short-lived protein...
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We don't sell the individual components of the kits separately because our product line is a kit concept line to ensure the use of certified products with quality controlled lot numbers that guarantee optimal results.
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One reason could be that your gene product is toxic to the cells. We recommend testing the construct for expression of the gene of interest by transient Nucleofection® of your cells. If the proportion of expressing cells drops between 4 and 48 hours...
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In general, all reporter genes can be used.For some applications (e.g. reporter gene experiments in various primary cells, experiments with late analysis time point due to starving and /or stimulation) a reporter gene with a longer half life than...
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