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Can heparin be excluded when culturing endothelial cells? What is the purpose of heparin in the endothelial cell culture media?

Heparin is added because it creates a better environment for FGF binding. Removing heparin for one passage should not have a noticeable effect, however for successive passaging, effects on proliferation may become more significant. It should be noted...

Are the human mesenchymal stem cells more prone to become differentiated down one lineage over the other?

MSCs are prone to differentiate down an adipogenic liniage and some may accumulate lipids in the growth media or the differentiation kits other than the adipogenic differentiation media. MSCs are especially prone to spontaneous adipogenic...

Do I need to shake the plate after adding the Cell Lysis Reagent during the ViaLight Assay?

No. Shaking the plate during this step can cause frothing which will deflect the light signal away from the detection unit, reducing the number of RLUs observed and producing an artificially low result.

What temperature should the ToxiLight, ViaLight and MycoAlert Assays be run at?

The optimal working temperature for all reagents is 22°C. If reagents have been refrigerated always allow time for them to reach room temperature before use.

What is the primary differentiation reagent for chondrogenic differentiation of mesenchymal stem cells?

Lonza recommends the addition of TGF-B3 to the Chondrogenic Differentiation Media to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a chondrogenic liniage.

What is the difference between the EBM and EBM-2 Basal Media? Can the EBM-2 Basal Media be substituted with the EBM phenol red free Basal Media?

The EBM™ and EBM™-2 Basal Media are essentially identical. The major difference between the EBM™ and EBM™-2 Basal Media is the concentration of phenol red (the EBM™-2 Basal Media has about 1/10th the amount of phenol red as the EBM™ Basal Media). The...

What is the difference between the EGM and EGM-2 growth media? What is the difference between the EGM-MV and EGM-2MV growth media?

The EBM™ and EBM™-2 basal media are essentially identical. The difference between the EGM™ and EGM™-2 growth media (or the EGM™-MV and EGM™-2MV growth media) are in the supplements. EGM™ and EGM™-MV growth media will contain the undefined reagent...

What is the difference between the EGM and EGM-MV Growth Media? What is the difference between the EGM-2 and EGM-2MV Growth Media?

Lonza endothelial cell media ending with –MV (such as the EGM™-MV and EGM™-2MV Growth Media) are typically designated for use with microvascular endothelial cells (with some exceptions). Lonza endothelial cell media not ending with –MV (such as the...

What percentage of most microvascular lots are lymphatic derived cells (LEC)? What percentage of most microvascular lots are blood vessel derived cells (BEC)?

Each lot of our Human Microvasculare Endothelial Cells - dermal (CC-2543 and CC-2516) and our Human Microvasculare Endothelial Cells - Lung (CC-2527) is tested for the percentage of LEC (double-positive for CD31+ and Podoplanin) and BEC (positive for...

Which isotype of VEGF is used in the EGM-2 and EGM-2MV Growth Media?

The isotype of VEGF is used in the EGM™-2 and EGM™-2MV Growth Media is isotype 165.
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