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Lonza's CD14+ monocytes are isolated first by obtaining mononuclear cells from peripheral blood via density gradient centrifugation. The CD14+ cells are then isolated form the mononuclear cell population using positive immunomagnetic selection....
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Seeding density is cell and experiment dependent, however cell seeding density in 3D cell culture can be important for behavior and differentiation. The RAFT™ 3D Cell Culture has a typical depth of 100-120µm. Therefore there is room for multiple...
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A broad range of techniques have been used with RAFT™ Cultures. The list includes imaging, biochemical, and histological techniques. To view technical and application notes on analytical techniques visit www.lonza.com/raft under product knowledge...
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Yes. A short protocol is included with RAFT™ Kits. Detailed instructions can be accessed on www.lonza.com/raft under product knowledge center tab.
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The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.
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Yes. The standard protocol is an excellent starting point for using the RAFT™ System however the system allows the researcher to modify the conditions to better suit their cells and experimental design. For instance the volume of collagen or the...
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Below is a list of compatible imaging techniques and systems with RAFT™ Cultures, depending on the type of cells being cultured. RAFT™ Culture Types: Cells that tend to maintain a round morphology or that form higher order structures such as...
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The stromal layer is composed of many cell types including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells, and macrophages.
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This is easily done using a pair of forceps. First, pass the forceps around the edge of the culture to help dislodge it from the bottom of the well, and then pinch the edge of the culture lightly to avoid damaging the culture. When in contact with...
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Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm sq in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...
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