Data Type
Cell Information
(857)
Citations
(4990)
FAQ
(515)
Culture Media
(61)
Category
Primary Cells and Media
(200)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin Detection
(54)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(135)
Cancer Research/Cell Biology
(77)
Immunotherapy / Hematology
(63)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(22)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(14)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
515 results sorted by
relevance
alphabetical
newest first
oldest first
C
a
n
I
a
p
p
l
y
m
o
r
e
t
h
a
n
o
n
e
p
r
o
g
r
a
m
(
t
r
a
n
s
f
e
c
t
i
o
n
c
o
n
d
i
t
i
o
n
)
p
e
r
p
l
a
t
e
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
S
y
s
t
e
m
?
With the 96-well Shuttle® system, it is possible to apply up to 96 different programs (transfection conditions) per 96-well Nucleocuvette® Plate. Therefore, optimization of Nucleofection® Conditions for a particular cell line can be...
W
h
e
n
I
t
r
a
n
s
f
e
c
t
e
d
m
y
c
e
l
l
s
,
e
r
r
o
r
8
a
p
p
e
a
r
e
d
o
n
t
h
e
d
i
s
p
l
a
y
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
I
I
N
,
I
I
S
o
r
2
b
.
W
h
a
t
i
s
t
h
e
r
e
a
s
o
n
?
Error 8 occurs when there has been an arc within the cuvette during application of the electrical program. The pulse application started but was interrupted because too much current was detected. Efficiency may be lower than expected due to the...
W
h
a
t
d
o
e
s
t
h
e
m
e
s
s
a
g
e
"
w
e
a
k
"
m
e
a
n
o
n
t
h
e
d
i
s
p
l
a
y
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
I
I
N
,
I
I
S
o
r
2
b
a
f
t
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
?
This message means that the system cut off the pulse before it was completed (usually this is because the conductivity of the solution is at the low end of the normal range). The pulse was applied; it is just going to be a little weaker than...
W
h
a
t
c
e
l
l
n
u
m
b
e
r
d
o
I
n
e
e
d
p
e
r
w
e
l
l
w
h
e
n
u
s
i
n
g
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
D
e
v
i
c
e
o
r
t
h
e
4
D
-
N
u
c
l
e
o
c
u
v
e
t
t
e
®
S
t
r
i
p
e
s
?
The cell number is very much dependent on the cell type you use. For high throughput Nucleofection®, we usually start with one fifth of the optimal amount recommended for the standard Nucleofection® in the 100µl cuvette. Currently the absolute...
W
h
a
t
i
s
t
h
e
a
m
o
u
n
t
o
f
D
N
A
n
e
e
d
e
d
p
e
r
w
e
l
l
w
h
e
n
u
s
i
n
g
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
D
e
v
i
c
e
?
The amount depends on the cell type and on the DNA construct. As a rule of thumb 400 ng of DNA per well should be applied. However, for some cells or constructs even 100 ng may be sufficient.
W
h
e
r
e
c
a
n
I
f
i
n
d
t
h
e
m
e
a
n
i
n
g
o
f
t
h
e
4
D
-
N
u
c
l
e
o
f
e
c
t
o
r
®
S
y
s
t
e
m
E
r
r
o
r
C
o
d
e
s
?
In the back of the respective manuals, there is a chart giving a brief description of the error codes. If more information is needed or an error code is recurrent, it is best to contact Scientific Support for advice to determine if there is a...
W
h
a
t
i
s
t
h
e
a
m
o
u
n
t
o
f
s
i
R
N
A
n
e
e
d
e
d
p
e
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
R
e
a
c
t
i
o
n
?
When performing siRNA-mediated knockdown experiments it is advisable to conduct a dose-response (concentration) analysis to determine the minimum siRNA concentration necessary for sufficient target knockdown on the mRNA, protein, or functional level....
W
h
i
c
h
p
i
p
e
t
t
e
t
i
p
s
a
r
e
c
o
m
p
a
t
i
b
l
e
w
i
t
h
t
h
e
9
6
-
w
e
l
l
S
h
u
t
t
l
e
®
N
u
c
l
e
o
c
u
v
e
t
t
e
®
P
l
a
t
e
s
?
We recommend using special tips, e.g. epT.I.P.S™ (Eppendorf, Hamburg, Germany) or TallTips™ (Matrix Technologies, Hudson, NH, USA). You can contact our Scientific Support Team for a list of compatible tips, including tips for pipetting robots.
A
c
c
o
r
d
i
n
g
t
o
w
h
i
c
h
s
t
a
n
d
a
r
d
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
S
y
s
t
e
m
c
e
r
t
i
f
i
e
d
?
The 4D-Nucleofector® Unit was tested by TÜV Rheinland Product Safety GmbH and found to be in compliance with the following standards:• IEC 61010-1The 4D-Nucleofector® Unit was tested by TÜV Rheinland of North America Inc. and found to be in...
C
a
n
I
f
r
e
e
z
e
t
h
e
9
6
-
w
e
l
l
N
u
c
l
e
o
c
u
v
e
t
t
e
®
P
l
a
t
e
s
?
Yes. Freezing of the plates at -20°C does not alter their properties.
PREVIOUS
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
PAGE
12
PAGE
13
PAGE
14
PAGE
15
NEXT