Data Type
Cell Information
(858)
Citations
(4983)
FAQ
(523)
Culture Media
(60)
Category
Primary Cells and Media
(211)
Transfection
(169)
Laboratory Instrumentation
(73)
Endotoxin Detection
(54)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(8)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(144)
Cancer Research/Cell Biology
(87)
Immunotherapy / Hematology
(63)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(21)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(13)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
523 results sorted by
relevance
alphabetical
newest first
oldest first
A
r
e
t
h
e
r
e
p
o
s
i
t
i
v
e
c
o
n
t
r
o
l
s
f
o
r
t
h
e
M
y
c
o
A
l
e
r
t
®
A
s
s
a
y
?
The MycoAlert® Assay Control Set (LT07-518) is available separately and includes a lyophilized positive control (1 ml) and assay buffer (2 ml) for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls...
W
h
a
t
p
r
o
t
o
c
o
l
s
h
o
u
l
d
I
u
s
e
f
o
r
N
u
c
l
e
o
f
e
c
t
i
o
n
®
o
f
p
a
t
i
e
n
t
d
e
r
i
v
e
d
b
l
o
o
d
s
a
m
p
l
e
s
,
e
.
g
.
l
e
u
k
e
m
i
a
o
r
l
y
m
p
h
o
m
a
c
e
l
l
s
?
Unfortunately, we do not have a ready-to-use protocol for Chronic lymphocytic leukemia (CLL) or other blood cell derived cancer cells. The cells often have a chromosomal aberration and show the properties of a cell line. Therefore we suggest to...
H
o
w
m
u
c
h
D
N
A
s
h
o
u
l
d
I
u
s
e
i
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
®
R
e
a
c
t
i
o
n
t
o
o
b
t
a
i
n
s
t
a
b
l
e
c
l
o
n
e
s
?
You should use the amount of DNA recommended in the optimized protocol for transient transfection. If obtaining a very high number of clones is important for your experiments, you can try increasing the amount of DNA. For further tips see our...
D
o
e
s
t
h
e
M
y
c
o
A
l
e
r
t
®
A
s
s
a
y
r
e
c
o
g
n
i
z
e
t
h
e
w
h
o
l
e
s
p
e
c
t
r
u
m
o
f
m
y
c
o
p
l
a
s
m
a
?
The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95% of all...
M
y
s
u
s
p
e
n
s
i
o
n
-
a
d
a
p
t
e
d
H
E
K
2
9
3
(
o
r
C
H
O
)
c
e
l
l
s
t
e
n
d
t
o
c
l
u
m
p
i
n
c
u
l
t
u
r
e
.
H
o
w
c
a
n
I
a
v
o
i
d
t
h
i
s
?
Efficient protein production experiments require growth of the host cell in single-cell suspension, something that is sometimes difficult to achieve since most established cell lines retain their adherent growth characteristics. Commercially...
D
o
b
a
c
t
e
r
i
a
l
e
a
d
t
o
f
a
l
s
e
p
o
s
i
t
i
v
e
M
y
c
o
A
l
e
r
t
®
R
e
s
u
l
t
s
?
NO - the lysis reagent in the MycoAlert® Assay is not strong enough to lyse bacteria / yeast. Also, the enzymes utilized are not very common in bacteria or yeast – but are universal among the mollicute family. Bacterial contamination is...
C
a
n
m
y
c
o
p
l
a
s
m
a
g
r
o
w
i
n
t
h
e
a
b
s
e
n
c
e
o
f
c
e
l
l
s
?
There is evidence to suggest that mycoplasma can survive in the absence of cells, some may even proliferate, but as a rule they are much more viable if cells are present.
D
o
e
s
t
h
e
G
F
P
s
e
q
u
e
n
c
e
i
n
t
h
e
p
m
a
x
G
F
P
™
c
o
n
t
r
o
l
p
l
a
s
m
i
d
h
a
s
a
s
t
o
p
c
o
d
o
n
?
Yes. The coding sequence ends with a stop codon (TGA/UGA).
I
s
i
t
p
o
s
s
i
b
l
e
t
o
s
t
o
r
e
r
e
c
o
n
s
t
i
t
u
t
e
d
M
y
c
o
A
l
e
r
t
®
/
M
y
c
o
A
l
e
r
t
®
P
L
U
S
R
e
a
g
e
n
t
a
n
d
S
u
b
s
t
r
a
t
e
?
For optimal assay conditions, reconstituted reagent and substrate should be used fresh within 2 hours after reconstitution.During this time they can be kept at room temperature.For the MycoAlert® Kit: Unused, reconstituted components can be aliquoted...
W
h
a
t
a
n
t
i
b
o
d
i
e
s
c
a
n
b
e
u
s
e
d
a
g
a
i
n
s
t
t
h
e
G
F
P
p
r
o
d
u
c
e
d
b
y
p
m
a
x
G
F
P
™
a
n
d
p
m
a
x
F
P
™
-
G
r
e
e
n
v
e
c
t
o
r
s
?
W
h
a
t
a
n
t
i
b
o
d
i
e
s
c
a
n
b
e
u
s
e
d
t
o
d
e
t
e
c
t
d
e
n
a
t
u
r
e
d
G
F
P
?
W
h
a
t
a
n
t
i
b
o
d
i
e
s
c
a
n
b
e
u
s
e
d
t
o
d
e
t
e
c
t
t
h
e
R
F
P
e
x
p
r
e
s
s
e
d
b
y
p
m
a
x
F
P
-
R
e
d
?
Most of the commercial antibodies for GFP do not detect GFP expressed from pmaxGFP or pmaxFP-Green vectors. The origin of the protein expressed by the pmaxGFP control plasmid and pmaxFP-Green is from the green fluorescent protein CopGFP, cloned from...
PREVIOUS
PAGE
7
PAGE
8
PAGE
9
PAGE
10
PAGE
11
PAGE
12
PAGE
13
PAGE
14
PAGE
15
NEXT