Data Type


Category

+ Show All

Research Area

+ Show All
723 results sorted by

How are Lonza's CD14+ monocytes isolated?

Lonza's CD14+ monocytes are isolated first by obtaining mononuclear cells from peripheral blood via density gradient centrifugation. The CD14+ cells are then isolated form the mononuclear cell population using positive immunomagnetic selection....

What cell seeding density should I use with the RAFT System?

Seeding density is cell and experiment dependent, however cell seeding density in 3D cell culture can be important for behavior and differentiation. The RAFT™ 3D Cell Culture has a typical depth of 100-120µm. Therefore there is room for multiple...

What analytical techniques have been used with the RAFT3D Cell Culture System?

A broad range of techniques have been used with RAFT™ Cultures. The list includes imaging, biochemical, and histological techniques. To view technical and application notes on analytical techniques visit www.lonza.com/raft under product knowledge...

Is there a standard protocol available for the RAFT 3D Cell Culture System?

Yes. A short protocol is included with RAFT™ Kits. Detailed instructions can be accessed on www.lonza.com/raft under product knowledge center tab.

What is the recommended media for culturing Lonza's bone marrow stromal cells?

The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.

Can I modify the RAFT Standard Protocol?

Yes. The standard protocol is an excellent starting point for using the RAFT™ System however the system allows the researcher to modify the conditions to better suit their cells and experimental design. For instance the volume of collagen or the...

How can I image cells in a RAFT 3D Cell Culture?

Below is a list of compatible imaging techniques and systems with RAFT™ Cultures, depending on the type of cells being cultured. RAFT™ Culture Types: Cells that tend to maintain a round morphology or that form higher order structures such as...

What cells make up the population of cells in Lonza's bone marrow stromal cells?

The stromal layer is composed of many cell types including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells, and macrophages.

Can I remove the RAFT 3D Cell Cultures from the plate in which they have been made?

This is easily done using a pair of forceps. First, pass the forceps around the edge of the culture to help dislodge it from the bottom of the well, and then pinch the edge of the culture lightly to avoid damaging the culture. When in contact with...

What is the recommended culturing protocol for Lonza's bone marrow stromal cells (2M-302)?

Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm sq in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...
PAGE 11