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What species of rat are the Clonetics™ Rat Astrocytes isolated from?

The rats are Sprague-Dawley.

Does the GFP sequence in the pmaxGFP™ control plasmid has a stop codon?

Yes. The coding sequence ends with a stop codon (TGA/UGA).

When can I start induction studies on Human Hepatocytes transfected with the Nucleofector®?

We recommend allowing the transfected hepatocytes to recover in Plating medium for 72 hours post-Nucleofection®.For induction studies (e.g. CYP3A4), the Plating medium is replaced with maintenance medium (William's E without FCS and EGF) and...

How long will your Clonetics™ rat DRG's (dorsal root ganglion cells) remain viable in culture?

The cells can remain viable in culture for about 28 days.

What is the lowest amount of Human Hepatocytes that can be used with the 96-well Shuttle® System or the 4D-Nucleofector® X-Unit?

Human hepatocytes do not like to be cultured and transfected at low densities. Therefore, using less than 100,000 cells per Nucleofection® is not recommended.

What antibodies can be used against the GFP produced by pmaxGFP™ and pmaxFP™-Green vectors? What antibodies can be used to detect denatured GFP? What antibodies can be used to detect the RFP expressed by pmaxFP-Red?

Most of the commercial antibodies for GFP do not detect GFP expressed from pmaxGFP or pmaxFP-Green vectors. The origin of the protein expressed by the pmaxGFP control plasmid and pmaxFP-Green is from the green fluorescent protein CopGFP, cloned from...

Do the Clonetics™ Rat and Mouse Astrocytes require an extracellular matrix for plating?

No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware

What kind of effects could a mycoplasma contamination have on my cells?

Mycoplasma contamination can: > Inhibit cell metabolism > Cause cell death > Induce morphological alterations > Alter DNA, RNA, and protein synthesis > Induce chromosomal aberrations > Cause increased sensitivity to inducers of...

What age are the rats that your Clonetics™ Rat DRGs are isolated from?

The Rat Neonatal Dorsal Root Ganglion (DRG) Neurons (R-DRG-505) are isolated from neonatal, about 2-3 days old.The Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons (R-eDRG-515) are isolated from embryonic rats (E14-15).

How can I detect mycoplasma contamination of my cells?

We recommend using the 20-minute MycoAlert® Mycoplasma Detection Kit from Lonza. The simple four-step MycoAlert® Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid...

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