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How can cells be cured of mycoplasma contamination?

Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells. The MycoZap™ Reagent eliminates...


Do you have any instructions available for using a hemocytometer for cell counting?

Yes. Our technical guideline "Cell Counting and Determination of Viability via Hemocytometer" can be found as associated pdf file in the link below.


When I transfected my cells, error 8 appeared on the display of the Nucleofector® IIN, IIS or 2b. What is the reason?

Error 8 occurs when there has been an arc within the cuvette during application of the electrical program. The pulse application started but was interrupted because too much current was detected. Efficiency may be lower than expected due to the...


What does the message "weak" mean on the display of the Nucleofector® IIN, IIS or 2b after Nucleofection®?

This message means that the system cut off the pulse before it was completed (usually this is because the conductivity of the solution is at the low end of the normal range). The pulse was applied; it is just going to be a little weaker than...

Are there any contaminating glial cells in your Clonetics rat or mouse embryonic neuronal cells?

There will be a small amount of glial cells in the culture, these are not considered a contaminating cell type. The glial cells are necessary to help the neurons form their axons and dendrites, synchronize the activity of the axons, and remove...

Where can I find the meaning of the 4D-Nucleofector® System Error Codes?

In the back of the respective manuals, there is a chart giving a brief description of the error codes. If more information is needed or an error code is recurrent, it is best to contact Scientific Support for advice to determine if there is a...

Do your Clonetics Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.
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