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Do mesenchymal stem cells cultured in TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium (MSCGM-CD) require an extracellular matrix/plate coating?

While, cells may be able to be cultured in TheraPEAK™ Chemically Defined Mesenchymal Stem Cell Growth Medium (MSCGM-CD™) without an extracellular matrix/plate coating, for peak performance, a 5ug/mL fibronectin coating should be used. Alternative...

How does the CytoSMART Lux 10X System work?

The CytoSMART™ Lux 10x System consists of three parts: a mini microscope, a tablet and online data storage and analysis (CytoSMART Connect Cloud). The mini microscope is placed inside the CO2 incubator. It is connected, through a cable that goes...

Does the basal media portion of the TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium (MSCGM-CD) contain proteins?

The basal media portion of the TheraPEAK™ Chemically Defined Mesenchymal Stem Cell Growth Medium (MSCGM-CD™), catalog no 00190620, contains Human Recombinant Insulin, Human Albumin, and Human Transferrin.

What are the CytoSmart  System applications?

The CytoSMART™ System can be used for many applications, including: Monitoring growth of adherent cells (stem cells, iPSCs, keratinocytes, etc.)Assays (growth assays, toxicity assays, migration assays, nanoparticle uptake)Troubleshooting (e.g....

What type of microscopy technique doe the CytoSMART System use?

The CytoSMART™ Device uses inverted bright-field microscopy. This means the sample is illuminated from the top and observed from below.

What testing methods are currently used to validate differentiation of Lonza's mesenchymal stem cells?

Lonza currently validates trilineage differentiation of human mesenchymal stem cells using the following quantitative assays: Adipogenic Differentiation: lipid vacuole binding with AdipoRed (catalog no. PT-7009) Osteogenic Differentiation:...

Can I detect fluorescence with the CytoSMART Device?

No. The CytoSMART™ Device is not a fluorescent microscope.

Where are Lonza's human Mesenchymal Stem Cells isolated from?

Lonza's human Mesenchymal Stem Cells (hMSCs) are isolated from bone marrow withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers. Please see the linked document for more information on Lonza's bone marrow donor...

What is the resolution of the CytoSMART Lux 10X?

The resolution is 3.5 µm, so it's perfect for any mammalian cells or any cell type you can see with a standard bright-field inverted microscope. Smaller cell types, e.g. algae, are not visible. The CytoSMART™ has a 1 megapixel CMOS camera....

What is the field of view of the CytoSMART LUX 10x Optics?

The field of view is 2.5 mm x 1.5 mm (3.75mm2)