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What donor characteristics are available for the diseased airway cells?

Most Clonetics™ Primary Cells have donor information on the Certificate of Analysis (CoA). This information usually includes age, sex, and race. Sometimes additional information may be obtained by contacting Lonza Scientific Support - if this...


Do I need to use a gelatin, fibronectin, collagen, or Matrigel coating when culturing my Clonetics Cells?

Most Clonetics™ Cells do not require an extracellular matrix when grown on tissue culture treated plastic – flasks, dishes, well plates (with the exception of hepatocytes, and the rat/mouse neuronal and dorsal root ganglion neurons, rat cardiac...


Do the Clonetics Rat and Mouse Astrocytes require an extracellular matrix for plating?

No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware

Your instructions for culturing of Clonetics Cells do not mention centrifuging the cells upon thawing. Won't the DMSO kill the cells?

We have seen on multiple occasions that the effects of centrifugation can be significantly more damaging than the effects of residual DMSO on primary cells. As such, unless specifically noted in the recommended culture protocol, instead of...

What age are the rats that your Clonetics Rat DRGs are isolated from?

The Rat Neonatal Dorsal Root Ganglion (DRG) Neurons (R-DRG-505) are isolated from neonatal, about 2-3 days old.The Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons (R-eDRG-515) are isolated from embryonic rats (E14-15).

I thawed and plated my ampoule of Clonetics Cells yesterday and most of the cells are floating. What happened?

Clonetics™ Cells will not be confluent on day one. After 24 hours, you will see few attached cells per field under the microscope. Aspirate off the media and the floating cells and replace with fresh, pre-warmed media. Those attached cells will begin...

Will the GFAP expression in your Clonetics Normal Human Astrocytes remain the same throughout the culture?

The GFAP expression decreases rapidly with passaging. The results that we give are performed in the 1st passage out of cryopreservation.

I've had my Clonetics Cells in culture for a few months, they're no longer growing and don't look like healthy cells, what's wrong?

All Clonetics™ Cells are primary cells with a finite life span. Most Clonetics™ Cells are guaranteed for 10 – 15 additional population doublings, which equates to about 2 – 3 passages. As cell death begins to occur, morphology and cell performance...
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