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What medium and which cytokines are recommended for Natural Killer (NK) cell culture?

For the cultivation of Natural Killer (NK) cells we recommend using the X-VIVO 15 medium.In regard to cytokines, this varies depending upon the application, but many cited publications indicate using IL-2 for activation and longer term culture.

Do Lonza's NK cells proliferate in culture?

Publications indicate that IL-2 and IL-21 can stimulate NK cell proliferation in addition to using a human serum supplemented medium.

Are Lonza's Natural Killer (NK) cells adherent in culture?

Resting Natural Killer cells grow in suspension, however, when IL-2 is added to the culture, they will adhere.

How is the B-ALI™ Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.

Where are Lonza’s Bronchial/Tracheal Epithelial cells isolated from?

Lonza’s Bronchial/Tracheal Epithelial Cells (both with and without Retinoic Acid; both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia and trachea...

Where are Lonza’s Small Airway Epithelial cells isolated from?

Lonza’s Small Airway Epithelial cells (both normal and diseased) are isolated from the distal portion of the lung in the 1 mm bronchiole area. These cells are isolated from airway tubules 1mm in diameter and smaller. Anything with a diameter greater...

In Air-Liquid-Interface (ALI) Culture, won't the apical surface of B-ALI™ Cells dry out?

No, the cells secrete fluid at the apical side but also moisture is drawn through the cell layer from the basal layer.

What passage are your B-ALI™ (Bronchial Air Liquid Interface) cells when plating on transwells?

The cryopreserved epithelial cells, that are screened and guaranteed for differentiation in B-ALI™ (Bronchial Air Liquid Interface) are in P1, the cells plated in transwells are P2. We do not recommend expansion of the cells before...

How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.

What is the difference between Lonza’s ALI screened airway epithelial cells and the non-screened cells?

All of Lonza’s airway epithelial cells receive standard quality control testing in monolayer culture. After cells have passed Lonza’s initial monolayer quality control testing, a few lots receive additional quality control testing to monitor...
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