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Do the Clonetics™ rat and mouse embryonic neuronal cells proliferate?

No, the neurons do not proliferate.

What buffer conditions give best resolution for agarose electrophoresis?

For small DNA fragments (<1,000 bp) when recovery is not necessary, we recommend the use of 1X TBE Buffer. Gels made with TBE Buffer give sharper bands than gels made with TAE Buffer. TBE results in better resolution for closely spaced DNA bands....

How does Lonza test for quality of the diseased airway cells?

Diseased Bronchial Epithelial Cells are characterized by morphology through serial passage and some are tested for differentiation using B-ALI™ Differentiation protocols, these are tested for TEER levels, ciliogenesis, and mucin production. Diseased...

What passage number are the Clonetics™ mouse and rat embryonic neuronal cells cryopreserved at?

The cells are cryopreserved at passage 0.

How should I cast my agarose gels to get the best resolution?

We usually cast agarose gels 3 mm to 4 mm thick. The gel volume needed can easily be estimated by measuring the surface area of the casting chamber, then multiplying by the gel thickness. Thinner gels can be cast on GelBond™ Support Film, and/or cast...

What medium do you recommend for the diseased airway cells?

The recommended medium for diseased cells is the same as for normal cells. BEGM™ for Diseased Bronchial Epithelial Cells, some lots are guaranteed in B-ALI™ Media. SmGM™-2 Medium is recommended for Diseased Bronchial Smooth Muscle Cells. FGM™-2...

Are there any contaminating glial cells in your Clonetics™ rat or mouse embryonic neuronal cells?

There will be a small amount of glial cells in the culture, these are not considered a contaminating cell type. The glial cells are necessary to help the neurons form their axons and dendrites, synchronize the activity of the axons, and remove...

The DNA bands on my agarose gel are sometimes wavy, but usually only in one or two lanes. What causes this?

Dried agarose on the comb teeth is a frequent cause of this problem. Prior to casting your gel, check the comb teeth for residual dried agarose.If not removed, this will attach to the newly cast agarose and fracture the well upon comb removal. This...

What donor characteristics are available for the diseased airway cells?

Most Clonetics™ Primary Cells have donor information on the Certificate of Analysis (CoA). This information usually includes age, sex, and race. Sometimes additional information may be obtained by contacting Lonza Scientific Support - if this...

Do your Clonetics™ Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.

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