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515 results sorted by
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Lonza is offering the following primary cells from single donors and from pooled donors:HUVEC (human umbilical vein endothelial cells)NHEK-neo (human epidermal keratinocytes)HMVEC-dNeo (human microvascular endothelial cells, dermal, neonatal)When...
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Lonza’s Smooth Muscle Cells are synthetic in their proliferating state. As the cells begin to differentiate, they become contractile.
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Once the cells have differentiated and started laying down bone matrix, we recommend assaying the cells immediately. If cells begin to delaminate (peel of the culture plate in layers), this would indicate that the cells are embedded in the calcium...
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Hydrocortisone is used for at least two purposes in Lonza’s primary cell culture media. Hydrocortisone (in combination with low serum concentrations) will inhibit the takeover of the culture by fibroblasts which may be present in small numbers from...
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Yes, insulin can be left out of the SmGM™-2 media for as long as 1-3 passages.Insulin is beneficial to the cells for long term growth (ie. 4 - 5 passages), however smooth muscle cells do survive quite well short term without insulin.Insulin is not a...
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Heparin is added because it creates a better environment for FGF binding. Removing heparin for one passage should not have a noticeable effect, however for successive passaging, effects on proliferation may become more significant. It should be noted...
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MSCs are prone to differentiate down an adipogenic lineage and some may accumulate lipids in the growth media or the differentiation kits other than the adipogenic differentiation media. MSCs are especially prone to spontaneous adipogenic...
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Lonza’s Normal Human Osteoblasts (NHOst) are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.
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For maximum re-differentiation and therefore maximum collagen production, Lonza’s Human Knee Articular Chondrocytes should be studied within the first passage after re-differentiation. Serial expansion in monolayer culture after re-differentiation...
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Lonza recommends the addition of TGF-B3 (PT-4124) to the Chondrogenic Differentiation Media (PT-3003) to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a...
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