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What microtiter plates would you recommend for running a MycoAlert® and MycoAlert® Plus Assay on a plate luminometer?

We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used with the...

How would you describe the morphology of Lonza’s Normal Human Osteoblasts (NHOst)?

Lonza’s Normal Human Osteoblasts (NHOst) generally appear fibroblastic. They are cuboidal in lower densities and become spindle shaped as they become confluent.

What buffer conditions give best resolution for agarose electrophoresis?

For small DNA fragments (<1,000 bp) when recovery is not necessary, we recommend the use of 1X TBE Buffer. Gels made with TBE Buffer give sharper bands than gels made with TAE Buffer. TBE results in better resolution for closely spaced DNA bands....

What is the percentage of purity of Lonza’s Normal Human Osteoblasts (NHOst) cells?

It is not easy to put an exact number to the purity of the NHOst populations because the tests used are all functional assays: alkaline phosphatase production and bone mineralization, which unless you have synchronized cultures, not all the cells are...

How should I cast my agarose gels to get the best resolution?

We usually cast agarose gels 3 mm to 4 mm thick. The gel volume needed can easily be estimated by measuring the surface area of the casting chamber, then multiplying by the gel thickness. Thinner gels can be cast on GelBond™ Support Film, and/or cast...

Are Lonza’s Normal Human Osteoblasts (NHOst) contact inhibited?

No, Lonza’s Normal Human Osteoblasts (NHOst) are not contact inhibited and tend to grow on top of each other after becoming confluent.

The DNA bands on my agarose gel are sometimes wavy, but usually only in one or two lanes. What causes this?

Dried agarose on the comb teeth is a frequent cause of this problem. Prior to casting your gel, check the comb teeth for residual dried agarose.If not removed, this will attach to the newly cast agarose and fracture the well upon comb removal. This...

What percentage of most microvascular lots are lymphatic derived cells (LEC)? What percentage of most microvascular lots are blood vessel derived cells (BEC)?

Each lot of our Human Microvasculare Endothelial Cells - dermal (CC-2543 and CC-2516) and our Human Microvasculare Endothelial Cells - Lung (CC-2527) is tested for the percentage of LEC (double-positive for CD31+ and Podoplanin) and BEC (positive for...

Why does Lonza recommend two different concentrations of the B-Glycerophosphate in the Normal Human Osteoblasts (NHOst) differentiation protocol?

Lonza R&D has found some NHOst cell strains to be more sensitive to B-Glycerophosphate than others. While it is necessary to differentiate, at too high of concentrations the cells will lift off dish, curl up and die. As such, Lonza recommends...

Where are Lonza’s lung microvascular endothelial cells (HMVEC-L) isolated from?

Lonza’s lung microvascular endothelial cells (HMVEC-L) are isolated from the tissue directly beneath the outer lining of the lung. All vessels isolated are smaller than can be seen with the naked eye.

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