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515 results sorted by
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While the complete formulation of all of Lonza’s specialty media are proprietary, the EBM™, EBM™-2, and EBM™ phenol red free basal media will all contain D-glucose (dextrose) [CAS number 50-99-7] at exactly 1.0 g/L (5.55 mM). There are no other...
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The bone marrow is collected from the iliac crest.
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Yes, but it depends on the platform. We would strongly recommend using the 4D-Nucleofector® or 96-well Shuttle™ System. The successful use of the 96-well Shuttle® System for transfection of P. falciparum has been published by Caro F, Miller...
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When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...
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Lonza uses sodium heparin as the anticoagulant at a final concentration of 100 units/mL.
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The fresh bone marrow is filtered only if visible clots are present. If the technical assistance notices clots in the marrow, they pour the marrow over a filter which removes the clot from the aspirate. No other manipulation of the product takes...
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Lonza’s aortic adventitial fibroblasts (AoAF) are isolated from the outer connective tissue layer of the aorta. The aortia is divided into three layers. The tunica intima is the inner endothelial layer, the tunica media is the layers of the smooth...
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Lonza’s lung fibroblasts are not taken from any specific area of the lung but instead are taken from an area that appears to be in good shape and is non-hemorrhagic. The pleural membrane is removed and the peripheral tissue is used. Enzymatic...
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Lonza use a Coulter Counter to perform the total cell count on the fresh, unprocessed bone marrow. Lonza does not perform viability testing on this product, but when shipped overnight, viability is typically around 80%.
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Lonza’s offers dermal fibroblasts from both adult and neonatal donors. Lonza’s adult dermal fibroblasts (NHDF-Ad) are usually isolated from tissue obtained from elective cosmetic surgical procedures. Generally, these procedures are abdominoplasties...
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