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When storing my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor (rFC), endotoxin detection kit the temperature of my refrigerator/freezer has been out of range. Are my reagents ok to use?

The normal storage temperature for LAL products is 2-8°C. The improper storage of the product for a short period of time should not cause a problem with the performance of the kit as long as the components have not been reconstituted. Lyophilized...

Where is Lonza's fresh bone marrow collected from?

The bone marrow is collected from the iliac crest.

How do I prevent keratinocyte terminal differentiation towards corneocytes? Can Lonzas keratinocytes be cultured without calcium?

Keratinocyte differentiation can be induced by calcium in the culture media. As such, lowering the calcium concentration in a serum free media will greatly lower the chances keratinocytes terminally differentiating towards corneocytes. While Lonza...

What is the difference between each of the types of HUVECs that Lonza offers?

When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...

What are the advantages of the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor (rFC), endotoxin detection assays over the rabbit test?

Lonza LAL and PyroGene™ rFC Endotoxin detection assays are faster, have greater sensitivity, show less variability,, are less expensive, and are an alternative to animal model testing. These assays allow higher throughput and can also be automated on...

How should I properly heat my Lonza QCL-1000 Endpoint LAL Bacterial Endotoxin Test (BET) Assay?

Incubation is another area where assay performance can be affected. We recommend the use of a dry bath and heat block combination that is capable of maintaining 37 ±1 °C. The temperature should be monitored with a calibrated thermometer and checked...

What type of heparin is used with fresh bone marrow?

Lonza uses sodium heparin as the anticoagulant at a final concentration of 100 units/mL.

What are the difference between the KGM®, KGM®-2, KGM-Gold® BulletKit®?

All three of the keratinocyte media that Lonza offers have similar basal media and supplements and all are serum free, however, slight variations and modifications have made the KGM-Gold® BulletKit® Lonza’s preferred keratinocyte...

How should I handle the reconstituted Lonza LAL PYROGENT Gel Clot Bacterial Endotoxin Testing (BET) Reagent?

Rehydrated LAL reagent may be stored for as long as 24 hours, but it must be kept at 2-8 °C. Do not pre-incubate lysate in the LAL test tubes before adding samples to them. This is not necessary and may cause sensitivity loss in the LAL reagent. If...

Can I still use Clonetics®/Poietics® Basal Media if it froze/partially froze during shipping?

Occasionally, though more frequently as outdoor temperatures begin to drop, media that Lonza recommends storing refrigerated will either completely freeze or partially freeze during shipping. While we do not suggest intentionally freezing and storing...
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