Incubation is another area where assay performance can be affected. We recommend the use of a dry bath and heat block combination that is capable of maintaining 37 ±1 °C. The temperature should be monitored with a calibrated thermometer and checked before each use of the dry bath. The rapid rate of the LAL reaction requires quick and uniform heat transfer. A warm air incubator is not as efficient at heat transfer as a dry bath. In a microtiter plate, edge effects can cause the different reaction wells to incubate at varying temperatures. These problems can be corrected by using an aluminum block plate holder.