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How should I prepare and handle my product samples for Bacterial Endotoxin Testing (BET) when using Lonza endotoxin detection assays? How do I adjust the pH?

As with handling of the lysate test reagents, the key word is cleanliness. Use aseptic and apyrogenic technique. Check the product for inhibition. Some products, especially members of the small volume parenteral family, may contain substances at...

Are Lonza Bacterial Endotoxin Detection (BET) reagents FDA approved?

All Lonza LAL based products, as well as the facility they are produced in, are FDA licensed.

What is the difference between Lonzas SAGM medium and the Growth Media portion of Lonzas S-ALI BulletKit Medium?

Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s SAGM™ medium just prior to differentiation using the...

Are the horseshoe crabs killed or injured during the harvest process?

Lonza takes great care to not harm the crabs during the harvesting of their blood. During harvesting blood is collected by puncturing the septum on the underside of the animal with a small needle. The animal will only give as much blood as it can...

What are the size of CD34+ Cells?

When resting, they are about 8-10 microns.

Does Lonza have products for oven validation depyrogenation studies?

Lonza offers Endotoxin Challenge Vials with part code 00192568 and High Potency Endotoxin with part code 00193763 for oven validation.

When preforming Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene recombinant Factor C (rFC), endotoxin detection kit to establish the potency of Endotoxin Challenge Vials (ECV) can I use a syringe to reconstitute the ECV and other assay reagents?

We do not recommend that a needle and syringe be used to reconstitute ECV or other LAL reagents. The plungers of plastic syringes are generally coated with a lubricant and tend to interfere with the assay. We recommend the tops be removed and diluent...

What media can the CD14+ Monocytes be cultured in to maintain the monocyte phenotype?

CD14+ Monocytes can be cultured in IMDM + 10% FBS to help maintain the monocyte phenotype. The cells will slowly become adherent but it can take a week to get good adherence. M-CSF (10 ng/ml) will induce a little proliferation and increase adherence....

Why do we recommend that MycoZap Reagent 1 treatment must be conducted in media containing a maximum of 5% (v/v) serum? What happens if by mistake a higher serum percentage was used?

As serum is complexing the MycoZap™ Reagent 1 you would decrease the effective concentration and thus inhibit the elimination reaction. If by mistake the elimination was started with higher serum concentration, no elimination took place. The process...

How many donors are pooled to create the pooled human cells?

Lonza is offering the following primary cells from single donors and from pooled donors:HUVEC (human umbilical vein endothelial cells)NHEK-neo (human epidermal keratinocytes)HMVEC-dNeo (human microvascular endothelial cells, dermal, neonatal)When...
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