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Human Skeletal Muscle Myoblasts usually begin to form multinucleated myotubes when the culture reaches 50-70% confluence. Myotubes are formed by the fusion of several to many myoblasts. Addition of differentiation medium, when the culture reaches...
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Dried agarose on the comb teeth is a frequent cause of this problem. Prior to casting your gel, check the comb teeth for residual dried agarose.If not removed, this will attach to the newly cast agarose and fracture the well upon comb removal. This...
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Lonza’s R&D estimates that the CASMC’s are around 30 µm in diameter when rounded/in suspension.
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The amount to load per well is variable. What is most important is how much DNA there is in the bands you wish to resolve. The least amount of DNA that can be consistently detected with ethidium bromide is about 10 ng. The most DNA you can have in a...
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While Lonza’s Human Aortic Smooth Muscle Cells (AoSMC) are isolated from either the ascending or descending aorta, it is usually the ascending aorta that is used. If the heart comes with the aorta, which happens most of the time, then the AoSMC are...
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We recommend running agarose gels at 4–10 volts/cm (cm is determined by measuring the interelectrode distance, not the gel length) under normal horizontal electrophoretic conditions.If the voltage is too high, band streaking, especially for DNA...
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There is no specification on whether the tissue for Lonza’s PASMCs comes from the area closest to the heart or the area closest to the lung.Generally, the tissue is pulled from the area branching off the heart so long as Lonza receives a long enough...
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CD14+ Monocytes can be cultured in IMDM + 10% FBS to help maintain the monocyte phenotype. The cells will slowly become adherent but it can take a week to get good adherence. M-CSF (10 ng/ml) will induce a little proliferation and increase adherence....
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NuSieve™ 3:1 Agarose is a standard melting temperature agarose. The resolution range for NuSieve™ 3:1 Agarose is 50 bp–1000 bp. NuSieve™ 3:1 Agarose is designed for analytical electrophoresis; its high gel strength also makes it ideal for use in...
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Lonza’s Smooth Muscle Cells are synthetic in their proliferating state. As the cells begin to differentiate, they become contractile.
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