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At what voltage do I need to run the FlashGel® Cassettes? Can I speed up or slow down the run?

We recommend that the FlashGel® DNA cassettes be run at 275 V and RNA cassettes at 225 V. We do not recommend running a gel at a higher voltage than 275 V.Runs at lower voltage for longer time periods may improve separation of larger fragments...

What is the FlashGel Mask?

The FlashGel™ Mask (Cat. No. 57020) is a flexible strip that slides under the FlashGel™ Cassette to block light from the second tier of wells when using double-tier cassettes.

Where are Lonzas aortic adventitial fibroblasts (AoAF) isolated from?

Lonza’s aortic adventitial fibroblasts (AoAF) are isolated from the outer connective tissue layer of the aorta. The aortia is divided into three layers. The tunica intima is the inner endothelial layer, the tunica media is the layers of the smooth...

Why do I need to store cell culture media in the dark?

Most tissue culturists will never observe effects on proliferating cells that can be attributed to light exposed media. However, several light-related factors may exert sufficient collective influence to cause the spurious results occasionally...

What is the purpose of the 5-bromo-2'-deoxyuridine (BrdU) when culturing rat cardiac myocytes?

5-bromo-2'-deoxyuridine (BrdU) is very efficient at inhibiting the proliferation of fibroblasts which are natively present at the time of isolation. Without the addition of the BrdU, the fibroblasts would overtake the culture.

Which Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection kit should I use?

There are a few different things you should consider before deciding which endotoxin detection kit to order. First of all, how many samples will you be testing, and how frequently? If you are going to be testing one or two samples, one time only,...

Can an alternate to the 5-bromo-2'-deoxyuridine (BrdU) be used to control the growth of fibroblasts when culturing rat cardiac myocytes?

At this time, we can only recommend the use of BrdU for controlling the growth of fibroblasts.When developing this product, we tried using cytosine beta-arabino furanoside (CAF) to inhibit fibroblast growth. While CAF successfully inhibited the...

What equipment do I need to run my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection assay?

Each endotoxin detection assay requires the use of different equipment. If running one of our PYROGENT™ Gel-Clot assays, a non-circulating waterbath or a dry heat block able to heat at 37°C is required. If running one of our kinetic assays,...

Can an alternate to the nitrocellulose be used as an extracellular matrix when culturing rat cardiac myocytes?

Lonza’s rat cardiac myocytes can also be cultured on fibronectin or laminin. Nitrocellulose was selected as the recommended substrate for its convenience and ease of use.

Can I get a Material Data Safety Sheet (MSDS) for my Lonza endotoxin detection Bacterial Endotoxin Testing (BET) kit, LAL or PyroGene Recombinant Factor C (rFC), and/or my accessory products?

All of our endotoxin detection products have been evaluated and deemed non-hazardous in accordance with 29 CFR 1910.1200, the Hazard Communication Standard, based on the percentage quantities of its constituents. Therefore, none of our endotoxin...
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