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515 results sorted by
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The maximum volume per well for the standard DNA and RNA cassettes is 5 µl. The recovery cassettes are designed to hold more and can be filled to 12 µl.
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With limited exceptions, Lonza does not test the diseased cells as part of our standard Quality Control testing in a manner which would validate the disease diagnosis. All of Lonza’s diseased airway cells are cultured using the same medium and...
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The amount to load per well is variable. What is most important is how much DNA there is in the bands you wish to resolve. The least amount of DNA that can be consistently detected with ethidium bromide is about 10 ng. The most DNA you can have in a...
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The FlashGel® System is very flexible. It is best to experiment with the system and adjust the levels to optimize your results.Optimal DNA load levels are 5-20 ng per band in a 5 µl load.DNA fragments = 5 ng are visible on the...
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Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s BEGM™ medium just prior to differentiation using the...
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We recommend running agarose gels at 4–10 volts/cm (cm is determined by measuring the interelectrode distance, not the gel length) under normal horizontal electrophoretic conditions.If the voltage is too high, band streaking, especially for DNA...
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We recommend that the FlashGel® DNA cassettes be run at 275 V and RNA cassettes at 225 V. We do not recommend running a gel at a higher voltage than 275 V.Runs at lower voltage for longer time periods may improve separation of larger fragments...
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Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s SAGM™ medium just prior to differentiation using the...
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NuSieve™ 3:1 Agarose is a standard melting temperature agarose. The resolution range for NuSieve™ 3:1 Agarose is 50 bp–1000 bp. NuSieve™ 3:1 Agarose is designed for analytical electrophoresis; its high gel strength also makes it ideal for use in...
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The FlashGel™ Mask (Cat. No. 57020) is a flexible strip that slides under the FlashGel™ Cassette to block light from the second tier of wells when using double-tier cassettes.
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