Data Type
Cell Information
(858)
Citations
(4969)
FAQ
(515)
Culture Media
(61)
Category
Primary Cells and Media
(200)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin and Pyrogen Testing
(54)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(16)
Cell Lines and Primary Cancer Cells
(10)
3D Cell Culture
(6)
Classical Media and Reagents
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(135)
Cancer Research/Cell Biology
(77)
Immunotherapy / Hematology
(63)
Gene Expression
(57)
Endotoxin Testing
(50)
Uncategorized
(46)
Stem Cells
(40)
Neurobiology
(27)
Cardiovascular
(26)
Respiratory Research
(26)
Molecular Biology
(25)
Toxicology
(22)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(14)
Drug Discovery
(7)
Parasitology
(2)
Gastroenterology
(1)
+ Show All
515 results sorted by
relevance
alphabetical
newest first
oldest first
D
o
I
n
e
e
d
t
o
s
h
a
k
e
t
h
e
p
l
a
t
e
a
f
t
e
r
a
d
d
i
n
g
t
h
e
C
e
l
l
L
y
s
i
s
R
e
a
g
e
n
t
d
u
r
i
n
g
t
h
e
V
i
a
L
i
g
h
t
™
A
s
s
a
y
?
No, this is not recommended.Shaking the plate during this step can - cause frothing which will deflect the light signal away from the detection unit, - reducing the number of RLUs observed - and producing an artificially low result.
W
h
a
t
t
e
m
p
e
r
a
t
u
r
e
s
h
o
u
l
d
t
h
e
T
o
x
i
L
i
g
h
t
™
,
V
i
a
L
i
g
h
t
™
a
n
d
M
y
c
o
A
l
e
r
t
™
A
s
s
a
y
s
b
e
r
u
n
a
t
?
The optimal working temperature for all reagents is 22°C.If reagents have been refrigerated always allow time for them to reach room temperature before use.
A
r
e
L
o
n
z
a
’
s
N
o
r
m
a
l
H
u
m
a
n
O
s
t
e
o
b
l
a
s
t
s
(
N
H
O
s
t
)
c
o
n
t
a
c
t
i
n
h
i
b
i
t
e
d
?
No, Lonza’s Normal Human Osteoblasts (NHOst) are not contact inhibited and tend to grow on top of each other after becoming confluent.
W
h
y
d
o
e
s
L
o
n
z
a
r
e
c
o
m
m
e
n
d
t
w
o
d
i
f
f
e
r
e
n
t
c
o
n
c
e
n
t
r
a
t
i
o
n
s
o
f
t
h
e
B
-
G
l
y
c
e
r
o
p
h
o
s
p
h
a
t
e
i
n
t
h
e
N
o
r
m
a
l
H
u
m
a
n
O
s
t
e
o
b
l
a
s
t
s
(
N
H
O
s
t
)
d
i
f
f
e
r
e
n
t
i
a
t
i
o
n
p
r
o
t
o
c
o
l
?
Lonza R&D has found some NHOst cell strains to be more sensitive to B-Glycerophosphate than others. While it is necessary to differentiate, at too high of concentrations the cells will lift off dish, curl up and die. As such, Lonza recommends...
W
h
a
t
i
s
t
h
e
d
i
f
f
e
r
e
n
c
e
b
e
t
w
e
e
n
t
h
e
E
B
M
™
a
n
d
E
B
M
™
-
2
B
a
s
a
l
M
e
d
i
a
?
C
a
n
t
h
e
E
B
M
™
-
2
B
a
s
a
l
M
e
d
i
a
b
e
s
u
b
s
t
i
t
u
t
e
d
w
i
t
h
t
h
e
E
B
M
™
p
h
e
n
o
l
r
e
d
f
r
e
e
B
a
s
a
l
M
e
d
i
a
?
The EBM™ and EBM™-2 Basal Media are essentially identical. The major difference between the EBM™ and EBM™-2 Basal Media is the concentration of phenol red (the EBM™-2 Basal Media has about 1/10th the amount of phenol red as the EBM™ Basal Media). The...
W
h
a
t
a
d
d
i
t
i
o
n
a
l
d
o
n
o
r
i
n
f
o
r
m
a
t
i
o
n
i
s
a
v
a
i
l
a
b
l
e
f
o
r
L
o
n
z
a
’
s
d
i
s
e
a
s
e
d
a
i
r
w
a
y
c
e
l
l
s
?
In addition to the standardly available donor information (age, gender, race), Lonza can provide the following additional information for most lots of our diseased airway cells: - smoking history (including volume and duration), - any medications...
W
h
a
t
i
s
t
h
e
d
i
f
f
e
r
e
n
c
e
b
e
t
w
e
e
n
t
h
e
E
G
M
™
a
n
d
E
G
M
™
-
2
g
r
o
w
t
h
m
e
d
i
a
?
W
h
a
t
i
s
t
h
e
d
i
f
f
e
r
e
n
c
e
b
e
t
w
e
e
n
t
h
e
E
G
M
™
-
M
V
a
n
d
E
G
M
™
-
2
M
V
g
r
o
w
t
h
m
e
d
i
a
?
The EBM™ and EBM™-2 basal media are essentially identical. The difference between the EGM™ and EGM™-2 growth media (or the EGM™-MV and EGM™-2MV growth media) are in the supplements.EGM™ and EGM™-MV growth media will contain the undefined reagent...
W
h
e
n
r
u
n
n
i
n
g
a
L
o
n
z
a
B
a
c
t
e
r
i
a
l
E
n
d
o
t
o
x
i
n
T
e
s
t
(
B
E
T
)
,
L
A
L
o
r
P
y
r
o
G
e
n
e
™
R
e
c
o
m
b
i
n
a
n
t
F
a
c
t
o
r
C
(
r
F
C
)
,
e
n
d
o
t
o
x
i
n
d
e
t
e
c
t
i
o
n
k
i
t
w
h
y
i
s
i
t
i
m
p
o
r
t
a
n
t
t
o
v
o
r
t
e
x
m
y
c
o
n
t
r
o
l
s
t
a
n
d
a
r
d
e
n
d
o
t
o
x
i
n
(
C
S
E
)
d
i
l
u
t
i
o
n
s
?
Endotoxin will adhere to glass surfaces, but this can be counteracted with proper vortexing to ensure that the solution you aliquot into your reaction tubes or microplate has the proper EU/ml concentration. As our package inserts state, the CSE vial...
W
h
a
t
i
s
t
h
e
d
i
f
f
e
r
e
n
c
e
b
e
t
w
e
e
n
t
h
e
E
G
M
™
a
n
d
E
G
M
™
-
M
V
G
r
o
w
t
h
M
e
d
i
a
?
W
h
a
t
i
s
t
h
e
d
i
f
f
e
r
e
n
c
e
b
e
t
w
e
e
n
t
h
e
E
G
M
™
-
2
a
n
d
E
G
M
™
-
2
M
V
G
r
o
w
t
h
M
e
d
i
a
?
Lonza endothelial cell media ending with –MV (such as the EGM™-MV and EGM™-2MV Growth Media) are typically designated for use with microvascular endothelial cells (with some exceptions).Lonza endothelial cell media not ending with –MV (such as the...
W
i
l
l
L
o
n
z
a
’
s
d
i
s
e
a
s
e
d
a
i
r
w
a
y
c
e
l
l
s
r
e
t
a
i
n
t
h
e
i
r
d
i
s
e
a
s
e
d
p
h
e
n
o
t
y
p
e
t
h
r
o
u
g
h
o
u
t
c
u
l
t
u
r
i
n
g
?
With limited exceptions, Lonza does not test the diseased cells as part of our standard Quality Control testing in a manner which would validate the disease diagnosis. All of Lonza’s diseased airway cells are cultured using the same medium and...
PREVIOUS
PAGE
22
PAGE
23
PAGE
24
PAGE
25
PAGE
26
PAGE
27
PAGE
28
PAGE
29
PAGE
30
NEXT