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Do I need to shake the plate after adding the Cell Lysis Reagent during the ViaLight Assay?

No, this is not recommended.Shaking the plate during this step can - cause frothing which will deflect the light signal away from the detection unit, - reducing the number of RLUs observed - and producing an artificially low result.

What temperature should the ToxiLight, ViaLight and MycoAlert Assays be run at?

The optimal working temperature for all reagents is 22°C.If reagents have been refrigerated always allow time for them to reach room temperature before use.

Are Lonzas Normal Human Osteoblasts (NHOst) contact inhibited?

No, Lonza’s Normal Human Osteoblasts (NHOst) are not contact inhibited and tend to grow on top of each other after becoming confluent.

Why does Lonza recommend two different concentrations of the B-Glycerophosphate in the Normal Human Osteoblasts (NHOst) differentiation protocol?

Lonza R&D has found some NHOst cell strains to be more sensitive to B-Glycerophosphate than others. While it is necessary to differentiate, at too high of concentrations the cells will lift off dish, curl up and die. As such, Lonza recommends...

What is the difference between the EBM and EBM-2 Basal Media? Can the EBM-2 Basal Media be substituted with the EBM phenol red free Basal Media?

The EBM™ and EBM™-2 Basal Media are essentially identical. The major difference between the EBM™ and EBM™-2 Basal Media is the concentration of phenol red (the EBM™-2 Basal Media has about 1/10th the amount of phenol red as the EBM™ Basal Media). The...

What additional donor information is available for Lonzas diseased airway cells?

In addition to the standardly available donor information (age, gender, race), Lonza can provide the following additional information for most lots of our diseased airway cells: - smoking history (including volume and duration), - any medications...

What is the difference between the EGM and EGM-2 growth media? What is the difference between the EGM-MV and EGM-2MV growth media?

The EBM™ and EBM™-2 basal media are essentially identical. The difference between the EGM™ and EGM™-2 growth media (or the EGM™-MV and EGM™-2MV growth media) are in the supplements.EGM™ and EGM™-MV growth media will contain the undefined reagent...

When running a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene Recombinant Factor C (rFC), endotoxin detection kit why is it important to vortex my control standard endotoxin (CSE) dilutions?

Endotoxin will adhere to glass surfaces, but this can be counteracted with proper vortexing to ensure that the solution you aliquot into your reaction tubes or microplate has the proper EU/ml concentration. As our package inserts state, the CSE vial...

What is the difference between the EGM and EGM-MV Growth Media? What is the difference between the EGM-2 and EGM-2MV Growth Media?

Lonza endothelial cell media ending with –MV (such as the EGM™-MV and EGM™-2MV Growth Media) are typically designated for use with microvascular endothelial cells (with some exceptions).Lonza endothelial cell media not ending with –MV (such as the...

Will Lonzas diseased airway cells retain their diseased phenotype throughout culturing?

With limited exceptions, Lonza does not test the diseased cells as part of our standard Quality Control testing in a manner which would validate the disease diagnosis. All of Lonza’s diseased airway cells are cultured using the same medium and...
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