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Do we know the severity of the asthma or the genotype of the cystic fibrosis donors?

Generally, information on the severity of asthma is not available. For our diseased cells from donors with cystic fibrosis, we usually have information on the genotype of the donor. Please contact our scientific support department for further...

How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.

Do I need a special media or protocol for culturing the Clonetics™ and Poietics™ Diseased Cells?

No, all of our diseased cells are isolated, expanded, and tested in the same media and using the same protocol as the normal counterpart cells.

Where are Lonza’s Human Skeletal Muscle Myoblasts (HSMM) cells isolated from?

The Human Skeletal Muscle Myoblasts are sourced from normal post-gestational tissue of cadavers from 1 day to 40 years old. The HSMMs are isolated from the quadriceps or psoas muscle.

How can Lonza’s Human Skeletal Muscle Myoblasts (HSMM) be differentiated to produce desmin?

Human Skeletal Muscle Myoblasts usually begin to form multinucleated myotubes when the culture reaches 50-70% confluence. Myotubes are formed by the fusion of several to many myoblasts. Addition of differentiation medium, when the culture reaches...

Approximately what size are the Coronary Artery Smooth Muscle Cells (CASMCs)?

Lonza’s R&D estimates that the CASMC’s are around 30 µm in diameter when rounded/in suspension.

What are some of the things I can customize on a precast agarose gel or molecular biology buffer?

Most aspects of a precast agarose gel can be customized, including: - well number, well spacing, well size/volume, - tier/row number, - gel size, - agarose type, agarose concentration, - stain, buffer, custom chemical additives, - packaging, labels,...

Where are Lonza’s Human Aortic Smooth Muscle Cells (AoSMC) isolated from?

While Lonza’s Human Aortic Smooth Muscle Cells (AoSMC) are isolated from either the ascending or descending aorta, it is usually the ascending aorta that is used. If the heart comes with the aorta, which happens most of the time, then the AoSMC are...

What media can the CD14+ Monocytes be cultured in to maintain the monocyte phenotype?

CD14+ Monocytes can be cultured in IMDM + 10% FBS to help maintain the monocyte phenotype. The cells will slowly become adherent but it can take a week to get good adherence. M-CSF (10 ng/ml) will induce a little proliferation and increase adherence....

Where are Lonza’s Pulmonary Artery Smooth Muscle Cells (PASMC) cells isolated from?

There is no specification on whether the tissue for Lonza’s PASMCs comes from the area closest to the heart or the area closest to the lung.Generally, the tissue is pulled from the area branching off the heart so long as Lonza receives a long enough...

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