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Is the MycoAlert® Assay validated for use in GMP environment?

No. The MycoAlert® Assay is sold for Research Use Only.

Can I also use the Lucetta® 2 Luminometer for other assays besides the MycoAlert® Mycoplasma Detection Assay?

Yes. In addition to the tailor-made MycoAlert® Mode it also has a "Single Read" mode which is suited for unprocessed luminescence readings, e.g. ATP-based cell proliferation or cytotoxicity assays, or Luciferase reporter gene assays.

I thawed and plated my Clonetics embryonic rat or mouse neuronal cells and there are a lot of dead cells. What happened?

Cell death will usually be observed during the first few days of growth, resulting in cell debris in the culture – this is normal. Cell cultivation should be continued and surviving cells should start to develop. By day 4, neurite networks will be...

What type of matrix should your Clonetics embryonic rat/mouse neural cells be seeded onto?

For optimal results, poly-D-lysine with laminin should be used, especially for the hippocampus cells.Poly-D-lysine without the laminin or poly-L-lysine could also be used for the rat and mouse neuronal cells.

Are the Clonetics embryonic rat/mouse neuronal cells from a single donor or pooled?

The cells are pooled from whole litters, including both males and females.

What is the age of the rats and mice the Clonetics embryonic neuronal cells are isolated from?

Both are of embryonic origin: the rats are E18 or E19 and the mice are E14 or E15.


What primary human diseased airway cells does Lonza offer?

Lonza's current portfolio for human diseased airway cells includes:- Bronchial Epithelial Cells- Lung Fibroblasts- Bronchial Smooth Muscle Cells...from asthmatic and COPD donors and if available from donors with Cystic fibrosis.An overview of...

What microtiter plates would you recommend for running a MycoAlert® and MycoAlert® Plus Assay on a plate luminometer?

We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used with the...

How is the disease state for Lonzas diseased airway cells determined?

The tissue received for all of Lonza’s diseased airway cells are obtained from donors which were diagnosed by an accredited physician prior to death. Frequently, Lonza will obtain information on medications taken related to the disease which will...
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