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Do Lonza's NK cells proliferate in culture?

Publications indicate that IL-2 and IL-21 can stimulate NK cell proliferation in addition to using a human serum supplemented medium.

How much DNA should I load per well of an agarose gel?

The amount to load per well is variable. What is most important is how much DNA there is in the bands you wish to resolve. The least amount of DNA that can be consistently detected with ethidium bromide is about 10 ng. The most DNA you can have in a...

Are Lonza's Natural Killer (NK) cells adherent in culture?

Resting Natural Killer cells grow in suspension, however, when IL-2 is added to the culture, they will adhere.

How is the B-ALI™ Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.

At what voltage should I run an agarose gel?

We recommend running agarose gels at 4–10 volts/cm (cm is determined by measuring the interelectrode distance, not the gel length) under normal horizontal electrophoretic conditions.If the voltage is too high, band streaking, especially for DNA...

In Air-Liquid-Interface (ALI) Culture, won't the apical surface of B-ALI™ Cells dry out?

No, the cells secrete fluid at the apical side but also moisture is drawn through the cell layer from the basal layer.

What is the difference between NuSieve™ 3:1 and NuSieve™ GTG™ Agaroses?

NuSieve™ 3:1 Agarose is a standard melting temperature agarose. The resolution range for NuSieve™ 3:1 Agarose is 50 bp–1000 bp. NuSieve™ 3:1 Agarose is designed for analytical electrophoresis; its high gel strength also makes it ideal for use in...

What passage are your B-ALI™ (Bronchial Air Liquid Interface) cells when plating on transwells?

The cryopreserved epithelial cells, that are screened and guaranteed for differentiation in B-ALI™ (Bronchial Air Liquid Interface) are in P1, the cells plated in transwells are P2. We do not recommend expansion of the cells before...

What is a GTG™ Agarose?

GTG™ stands for Genetic Technology Grade™. GTG™ Grade Agarose is recommended for preparative DNA electrophoresis, or when further enzymatic manipulation of DNA is required. These agaroses are extensively tested to ensure maximum compatibility with...

How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.

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