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729 results sorted by
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M
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We recommend using negative selection (depletion) because your purified cells are â??untouchedâ?, not influenced, and strictly not activated. Positive selection may cause increasing amounts of dead cells and could also lead to activation of the...
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The duration time of a program is dependent on the number of the program. The duration is also dependent on how long the Nucleofector™ Device has been turned on as well as the number of Nucleofections you have done in the last couple of minutes. When...
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Yes, this is a routine observation. Fluorescent fusion proteins can appear less bright than the fluorescent protein itself. This may be due to differences in protein folding of the fused protein.
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The recommended amount of DNA per 100 µl reaction is 0.5µg-5 µg. No carrier is needed.
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K
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No, it is not. The PEG purification step removes additional endotoxin from the DNA preparation. The pmaxGFP reporter plasmid supplied in each kit is highly purified and endotoxin levels are <0.01E.U./µg DNA (which means <1pg endotoxin/µg DNA).
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It is very critical. Waiting too long for analysis post transfection can lead to loss of peak expression. The optimal time for analysis is related to two factors: stability of protein being expressed and transfection method. For a short-lived...
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?
No. The electrical parameters provided by the Nucleofector™ Device are optimized for the cuvettes contained in the Nucleofector™ Kits. The cuvettes are single-use only. Using the cuvettes more than once will result in higher cell death and lower...
D
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?
Yes. To check the quality of your DNA we strongly recommend determining the A260:A280 ratio. It should be ideally 1.8 - 2.0 for a good DNA preparation, but least 1.6.
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?
We don't sell the individual components of the kits separately because our product line is a kit concept line to ensure the use of Amaxa™ certified products with quality controlled lot numbers that guarantee optimal results.
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?
Yes. You should verify the integrity of your DNA on an agarose gel to see if it is degraded. Compare undigested plasmid to plasmid DNA digested with a suitable single cut restriction enzyme to linearize. Supercoiled plasmid will run faster than...
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