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Yes. To check the quality of your DNA we strongly recommend determining the A260:A280 ratio. It should be ideally 1.8 - 2.0 for a good DNA preparation, but least 1.6.
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Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells. The MycoZap™ Reagent eliminates...
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The rats are Sprague-Dawley and the mice are CD1 or C57.
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Yes. You should verify the integrity of your DNA on an agarose gel to see if it is degraded. Compare undigested plasmid to plasmid DNA digested with a suitable single cut restriction enzyme to linearize. Supercoiled plasmid will run faster than...
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Yes. Please click on the associated pdf file for more information.
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No, the neurons do not proliferate.
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Yes. There are several publications available showing stable transfection of an shRNA vector: Cai S et al. (2006) Nat Genet 38:1278-88 (D10.G4.1 cells) Guo F et al. (2005) Cancer Res 65:10536-44 (K562) Hideshima T (2006) Blood 107: 4053-62 (U937)...
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Error 8 occurs when there has been an arc within the cuvette during application of the electrical program. The program was begun but was interrupted because too much current was detected. Efficiency may be lower than expected due to the incomplete...
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The cells are cryopreserved at passage 0.
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This message means that the device cut off the pulse before it was completed (usually this is because the conductivity of the solution is at the low end of the normal range). The pulse was applied; it is just going to be a little weaker than...
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