Data Type

Category

+ Show All

Research Area

+ Show All
515 results sorted by

I thawed and plated my Clonetics™ embryonic rat or mouse neuronal cells and there are a lot of dead cells. What happened?

Cell death will usually be observed during the first few days of growth, resulting in cell debris in the culture – this is normal. Cell cultivation should be continued and surviving cells should start to develop. By day 4, neurite networks will be...

What type of matrix should your Clonetics™ embryonic rat/mouse neural cells be seeded onto?

For optimal results, poly-D-lysine with laminin should be used, especially for the hippocampus cells.Poly-D-lysine without the laminin or poly-L-lysine could also be used for the rat and mouse neuronal cells.

What are some of the things I can customize on a precast agarose gel or molecular biology buffer?

Most aspects of a precast agarose gel can be customized, including: - well number, well spacing, well size/volume, - tier/row number, - gel size, - agarose type, agarose concentration, - stain, buffer, custom chemical additives, - packaging, labels,...

Are the Clonetics™ embryonic rat/mouse neuronal cells from a single donor or pooled?

The cells are pooled from whole litters, including both males and females.

What is the age of the rats and mice the Clonetics™ embryonic neuronal cells are isolated from?

Both are of embryonic origin: the rats are E18 or E19 and the mice are E14 or E15.

What species of rats and mice are your Clonetics™ embryonic neuronal cells isolated from?

The rats are Sprague-Dawley and the mice are CD1 or C57.

What microtiter plates would you recommend for running a MycoAlert® and MycoAlert® Plus Assay on a plate luminometer?

We would recommend either Porvair Sciences 204003 or Corning Costar 3912 plates to use with the MycoAlert® and MycoAlert® Plus Assay. Both of these are solid white 96-well plates and give low background and optimal results when used with the...

What buffer conditions give best resolution for agarose electrophoresis?

For small DNA fragments (<1,000 bp) when recovery is not necessary, we recommend the use of 1X TBE Buffer. Gels made with TBE Buffer give sharper bands than gels made with TAE Buffer. TBE results in better resolution for closely spaced DNA bands....

Do the Clonetics™ rat and mouse embryonic neuronal cells proliferate?

No, the neurons do not proliferate.

What passage number are the Clonetics™ mouse and rat embryonic neuronal cells cryopreserved at?

The cells are cryopreserved at passage 0.
PAGE 15

Privacy | Legal | About Lonza

© Lonza. All rights reserved.