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I thawed and plated my Clonetics embryonic rat or mouse neuronal cells and there are a lot of dead cells. What happened?

Cell death will usually be observed during the first few days of growth, resulting in cell debris in the culture – this is normal. Cell cultivation should be continued and surviving cells should start to develop. By day 4, neurite networks will be...

When I transfected my cells, error 8 appeared on the display of the Nucleofector® IIN, IIS or 2b. What is the reason?

Error 8 occurs when there has been an arc within the cuvette during application of the electrical program. The pulse application started but was interrupted because too much current was detected. Efficiency may be lower than expected due to the...

What are SingleQuots Kits?

A SingleQuots™ Supplements and Growth Factor Kit contains several vials that may include growth factors, FBS, and antibiotics in pre-measured aliquots - designed to be added to a bottle of basal medium. The concentrations of these factors are...

What type of matrix should your Clonetics embryonic rat/mouse neural cells be seeded onto?

For optimal results, poly-D-lysine with laminin should be used, especially for the hippocampus cells.Poly-D-lysine without the laminin or poly-L-lysine could also be used for the rat and mouse neuronal cells.

Do I need to add antibiotics to your Clonetics Media? What is GA-1000?

For most Clonetics™ Media, gentamicin-amphotericin-B mixture is provided at a final concentration of 30 µg/ml gentamicin and 15 ng/ml amphotericin-B. This antibiotic mixture is provided in a red-capped vial labeled GA-1000.

Are the Clonetics embryonic rat/mouse neuronal cells from a single donor or pooled?

The cells are pooled from whole litters, including both males and females.

Can I use a medium other than the one you offer to culture Clonetics® Cells?

We have an optimized medium for each Clonetics® Cell type. The cells are grown and tested in their specific medium system. Lonza guarantees the performance of Clonetics® cells only if appropriate Clonetics® media and reagents are used...

What is the age of the rats and mice the Clonetics embryonic neuronal cells are isolated from?

Both are of embryonic origin: the rats are E18 or E19 and the mice are E14 or E15.

What kit sizes are available for the 4D-Nucleofector® X Unit?

We offer two different kit sizes for the 100 µl Nucleocuvette® Format (12 and 24 reactions) and one size for the 20 µl 16-well Nucleocuvette® Strip Format (32 reactions). 

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