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Will Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) express collagen Type X? Are Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) hypertrophic following re-differentiation?

Lonza guarantees that the Normal Human Articular Chondrocytes (NHAC-kn) will express collagen Type II and sulfated proteoglycans (via Alcian Blue) upon re-differentiation. Lonza’s recommended differentiation protocol does induce hypertrophic...

Can Lonza’s PrEC cells be co-cultured with Lonza’s PrSC cells?

While Lonza has never attempted to co-culture Prostate Epithelial Cells (PrEC) and Prostate Stromal Cells (PrSC) together in-house, the recommendation of Lonza Research & Development would be to plate and culture the PrEC and PrSC cells together in...

Where are Lonza’s PrSC cells isolated from? What cells will be in the PrSC population?

Lonza’s Prostate Stromal Cells (PrSC) are isolated from the stroma of the prostate. Lonza’s Prostate Stromal Cells (PrSC) are a mixed population of cells, however, the majority of the cells in this population are fibroblastic in origin. It is not...

Where are Lonza’s PrEC cells isolated from?

Lonza obtains our Lonza’s Prostate Epithelial Cells (PrEC) from the glandular epithelium (parenchymal portion) of the prostate which spans the entire prostate and not from one specific region.

What tips do you have for ensuring my large proteins transfer efficiently during a tank transfer for western blot?

Choosing a gradient gel can be beneficial when transfer of a larger protein is necessary so the acrylamide concentration surrounding the larger protein of interest is lower. Constructing the transfer sandwich with the gel upside down such that the...

Where are Lonza’s PrSMC cells isolated from?

Lonza’s Prostate Smooth Muscle Cells (PrSMC) are isolated from the stroma and the vasculature of the prostate. Unfortunately, there is no way to distinguish between smooth muscle cells from the stroma and smooth muscle cells from the vasculature and...

Where are Lonza's Bronchial Smooth Muscle Cells isolated from?

Lonza’s Bronchial Smooth Muscle Cells (both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia and trachea just off of the bifurcation.

How long can Lonza’s normal human astrocytes survive without fetal bovine serum (FBS)?

Lonza recommends culturing normal human astrocytes (NHA) in Lonza’s AGM™ Astrocyte Growth Medium which contains fetal bovine serum (FBS) at a final concentration of 3.0%. For some applications, researchers may need to serum starve their cells. Under...

Are Lonza’s normal human astrocytes Type I or Type II in origin?

Type I astrocytes are protoplasmic and isolated from gray matter, whereas Type II astrocytes are fibrous and isolated from white matter. As Lonza’s normal human astrocytes (NHA) are primarily isolated from the cerebral cortex (or forebrain from...

When culturing Lonza’s normal human astrocytes, will glial fibrillary acidic protein (GFAP) continue to be expressed throughout serial passages?

Lonza Research & Development and Quality Control have seen that glial fibrillary acidic protein (GFAP) expression decreases rapidly with passaging. Lonza tests GFAP expression in the first passage from cryopreservation and can therefore only...

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