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How are Pancreatic Islets Shipped?

Iselts are shipped ambient, overnight delivery and will arrive to the customer 24-48 hours after isolation. Product sizes of 100 K, 20 K, and 10 K IEQ are shipped in a Wilson Wolf G-Rex100 gas permeable cell culture device containing 500 ml of media...

How do I handle my Lonza Control Standard Endotoxin (CSE) for Bacterial Endotoxin Testing (BET)?

Lyophilized E. coli endotoxin should be stored at 2-8°C. Endotoxin solutions are generally very stable upon storage except at more dilute concentrations. Rehydrated E. coli endotoxin should be stored refrigerated only (at 2-8°C and not frozen. The...

Where can Lonza ship the Pancreatic Islet?

Due to time restraints in custom, Lonza Pancreatic Islets are currently only available to North America and Europe.

When performing the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene recombinant Factor C (rFC), endotoxin detection kit how do I know my pipettes are working adequately?

Check your pipetting accuracy. This is important in every phase of LAL testing. Make sure you are adding the correct amounts of material. Good quality graduated pipettes are already calibrated by the manufacturer, but it is up to the user to use...

How long is the media that Lonza ships with the Pancreatic Islets guaranteed?

The media that Lonza ships with the Pancreatic Islets is guaranteed for one week from the date of arrival when appropriately stored/used.

When performing the Lonza Bacterial Endotoxin Test (BET), LAL gel clot endotoxin detection kit how do I know if I have a positive result for gel clot?

A positive result is a firm gel which maintains its integrity through inversion to 180°. Resist the temptation to look at any of the test tubes before the hour is up. When reading your test results after the one-hour incubation gently withdraw each...

How is the Islet cell identity (IEQ) for Lonza Pancreatic Islets determined?

Diphenylthiocarbazone (DTZ) is utilized for discrimination of islets from non-islet tissue and for islet enumeration. DTZ selectively binds to the zinc-insulin complex in islet beta cell granules, and results in a red staining of the islets. The...

When performing the Lonza LAL endpoint chromogenic QCL-1000 Bacterial Endotxoin Test (BET) how can I spike my sample for positive product control?

A spiked aliquot of the test sample (or dilution) may be prepared as follows: Method #1: Transfer 10 ml of a 2.0 EU/ml solution into each of the appropriate PPC wells in the 96-well plate or reaction tubes. Each well will now contain 0.02 EU/well....

Is there a difference between the media Lonza Pancreatic Islets are shipped in and the additional media provided in the 500 ml bottle?

No, the shipping and the culture media for Lonza Pancreatic Islets are exactly the same.

How should I prepare and handle my product samples for Bacterial Endotoxin Testing (BET) when using Lonza endotoxin detection assays?

As with handling of the lysate test reagents, the key word is cleanliness. Use aseptic and apyrogenic technique. Check the product for inhibition. Some products, especially members of the small volume parenteral family, may contain substances at...