Data Type
Cell Information
(876)
Citations
(5047)
FAQ
(539)
Culture Media
(62)
Category
Primary Cells and Media
(198)
Transfection
(170)
Laboratory Instrumentation
(73)
Endotoxin and Pyrogen Testing
(70)
Bioassay
(44)
Electrophoreses and Analysis
(32)
Mycoplasma Detection and Prevention
(32)
Serum-free and Speciality Media
(25)
Cell Lines and Primary Cancer Cells
(9)
Classical Media and Reagents
(7)
3D Cell Culture
(6)
Cell Services
(3)
Bioprocess Containers
(2)
Informatics for QC Microbiology
(2)
Live Cell Imaging
(2)
Uncategorized
(2)
Protozoa
(1)
+ Show All
Research Area
Basic Research
(139)
Cancer Research/Cell Biology
(80)
Immunotherapy / Hematology
(61)
Gene Expression
(60)
Endotoxin Testing
(49)
Uncategorized
(45)
Stem Cells
(40)
Cardiovascular
(27)
Neurobiology
(27)
Respiratory Research
(27)
Molecular Biology
(26)
Toxicology
(23)
Regenerative medicine
(20)
Dermatology/Tissue Engineering
(15)
Drug Discovery
(8)
Parasitology
(3)
Gastroenterology
(2)
+ Show All
539 results sorted by
relevance
alphabetical
newest first
oldest first
A
c
c
o
r
d
i
n
g
t
o
w
h
i
c
h
s
t
a
n
d
a
r
d
i
s
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
S
y
s
t
e
m
c
e
r
t
i
f
i
e
d
?
The 4D-Nucleofector® Unit was tested by TÜV Rheinland Product Safety GmbH and found to be in compliance with the following standards:• IEC 61010-1The 4D-Nucleofector® Unit was tested by TÜV Rheinland of North America Inc. and found to be in...
H
o
w
l
o
n
g
d
o
e
s
i
t
t
a
k
e
t
o
o
b
t
a
i
n
s
t
a
b
l
e
t
r
a
n
s
f
e
c
t
a
n
t
s
?
Depending on individual cell type and doubling rate, selection of stable transfectants will take between 7 and 28 days. Expansion and characterization of single cell clones will take several weeks in addition.
C
a
n
m
y
c
o
p
l
a
s
m
a
g
r
o
w
i
n
t
h
e
a
b
s
e
n
c
e
o
f
c
e
l
l
s
?
There is evidence to suggest that mycoplasma can survive in the absence of cells, some may even proliferate, but as a rule they are much more viable if cells are present.
I
s
i
t
t
y
p
i
c
a
l
f
o
r
t
h
e
f
l
u
o
r
e
s
c
e
n
c
e
o
b
s
e
r
v
e
d
f
r
o
m
m
y
t
a
g
g
e
d
p
r
o
t
e
i
n
t
o
a
p
p
e
a
r
l
e
s
s
t
h
a
n
t
h
e
e
m
p
t
y
f
l
u
o
r
e
s
c
e
n
t
v
e
c
t
o
r
?
Yes, this is a routine observation.Fluorescent fusion proteins can appear less bright than the fluorescent protein itself. This may be due to differences in protein folding of the fused protein.
I
s
i
t
p
o
s
s
i
b
l
e
t
o
s
t
o
r
e
r
e
c
o
n
s
t
i
t
u
t
e
d
M
y
c
o
A
l
e
r
t
®
/
M
y
c
o
A
l
e
r
t
®
P
L
U
S
R
e
a
g
e
n
t
a
n
d
S
u
b
s
t
r
a
t
e
?
For optimal assay conditions, reconstituted reagent and substrate should be used fresh within 2 hours after reconstitution.During this time they can be kept at room temperature.For the MycoAlert® Kit: Unused, reconstituted components can be aliquoted...
I
n
s
t
a
b
l
e
c
e
l
l
l
i
n
e
g
e
n
e
r
a
t
i
o
n
,
I
h
a
v
e
a
v
e
r
y
h
i
g
h
t
r
a
n
s
f
e
c
t
i
o
n
e
f
f
i
c
i
e
n
c
y
,
b
u
t
m
o
s
t
o
f
m
y
c
e
l
l
s
d
i
e
d
u
r
i
n
g
s
e
l
e
c
t
i
o
n
.
I
s
t
h
i
s
t
o
b
e
e
x
p
e
c
t
e
d
?
This is the normal pattern you should expect to see. Only a small proportion (1/10,000 to 1/100) of all transfected cells will integrate the transfected DNA into their genome and become stable transfectants. The remaining cells lose the transfected...
I
n
m
y
N
u
c
l
e
o
f
e
c
t
i
o
n
®
E
x
p
e
r
i
m
e
n
t
,
h
o
w
c
r
i
t
i
c
a
l
i
s
t
h
e
t
i
m
e
p
o
i
n
t
f
o
r
a
n
a
l
y
s
i
s
p
o
s
t
t
r
a
n
s
f
e
c
t
i
o
n
?
It is very critical. Waiting too long for analysis post transfection can lead to loss of peak expression. The optimal time for analysis is related to two factors: stability of protein being expressed and transfection method. For a short-lived protein...
W
h
a
t
s
e
l
e
c
t
i
o
n
m
a
r
k
e
r
s
c
a
n
I
u
s
e
f
o
r
t
h
e
g
e
n
e
r
a
t
i
o
n
o
f
s
t
a
b
l
e
c
e
l
l
l
i
n
e
s
?
The most commonly used marker is the neomycin phosphotransferase (neo) gene that confers resistance to G418 to eukaryotic cells. Other markers are Puromycin, Hygromycin, Zeocin, or the HPRT gene that can be used in HPRT-deficient cells.
D
o
w
e
s
e
l
l
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
S
o
l
u
t
i
o
n
(
o
r
a
n
y
o
t
h
e
r
c
o
m
p
o
n
e
n
t
o
f
t
h
e
N
u
c
l
e
o
f
e
c
t
o
r
®
K
i
t
)
s
e
p
a
r
a
t
e
l
y
?
We don't sell the individual components of the kits separately because our product line is a kit concept line to ensure the use of certified products with quality controlled lot numbers that guarantee optimal results.
H
o
w
m
u
c
h
s
a
m
p
l
e
d
o
y
o
u
n
e
e
d
f
o
r
a
c
c
u
r
a
t
e
r
e
s
u
l
t
s
w
i
t
h
M
y
c
o
A
l
e
r
t
®
A
s
s
a
y
?
D
o
y
o
u
n
e
e
d
c
e
l
l
s
o
r
j
u
s
t
s
u
p
e
r
n
a
t
a
n
t
?
The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant as sample.Smaller initial aliquots of media may be taken to start with if necessary.
PREVIOUS
PAGE
9
PAGE
10
PAGE
11
PAGE
12
PAGE
13
PAGE
14
PAGE
15
PAGE
16
PAGE
17
NEXT